Long-read sequencing uncovers key regulatory genes involved in the differentiation of preadipocytes of Chinese red steppe cattle by Wenwen Fang, Shaoying Yang, Xuanyu Li, Asadollahpour Nanaei, Yu Liu, Yang Cao, Cheng Xiao, Xin Li, Haiguo Jin, Yumin Zhao, Yang Cao

“…At the same time, based on diverse expression patterns in 3 periods (early, middle and late), we identified 754 significantly differentially expressed genes (DEGs) and 932 significantly differentially expressed transcripts (DETs) in the first contrast group (0d_vs_4d), 197 significantly DEGs and 297 significantly DETs in the second contrast group (0d_vs_8d), 465 and 555 significantly DEGs and DETs in the third contrast group (4d_vs_8d). …”
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Dissemination and phenotypic characterization of ESBL-producing Escherichia coli in Indonesia by Tati Ariyanti, Suhaemi Suhaemi, Sri Mulyati, Sukatma Sukatma, Sumirah Sumirah, Susan Maphilindawati Noor, Faidah Rachmawati, Prima Mei Widiyanti, Eddy Sukmawinata, Andriani Andriani, Eni Kusumaningtyas, Aswin Rafif Khairullah

“…Unveiling this problematic bacteria's prevalence and resistance patterns in animals is crucial for formulating effective control strategies and safeguarding public health. …”
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Lipidomic analyses reveal the dysregulation of oxidized fatty acids (OxFAs) and acyl-carnitines (CARs) in major depressive disorder: a case-control study by Lei He, Na Duan, Chong Wang, Ru Shan, Jing Li, Lin Wang, Qiuli Liu, Junwei Tao, Li Liu, Xiaoliang Ma, Bing Cao

“…Additionally, we found that 8 types of acyl-carnitines (CARs) decreased, primarily comprising singular carbon chain structures, while 3 types of CARs increased, all with numerical carbon chain patterns. Lipid profiles yield a high area under the receiver operating characteristic (ROC) curve for differentiating MDD, with the most prominent ROC ranking being mostly attributed to OxFAs. …”
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Multi-omics analysis of methylmalonic acidemia caused by a non-coding region variant in MMAA gene combined with uniparental disomy by HUO Xiaoyan, LUO Xiaomei, YE Xiantao, SUN Yu, YU Yongguo, LIANG Lili, FAN Yanjie

“…Subsequently, RNA sequencing (RNA-seq) and whole genome sequencing (WGS) were conducted to comprehensively analyze the child's genetic variants, their origins and potential inheritance patterns.Results·No pathogenic variants associated with the patient's phenotype were identified through the MMA Panel or standard WES analysis. …”
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Ventilator-associated nosocomial pneumonia in intensive care units in Malaysia by Supaletchimi Gopal Katherason, Lin Naing, Kamarudin Jaalam, Kamarul Imran Musa, Nik Abdullah Nik Mohamad, Subramaniar Aiyar, Kavita Bhojani, Najah Harussani, Aisai Abdul Rahman, Asma Ismail

“…We conducted a three-year prospective study on the incidences of ventilator-associated pneumonia (VAP), risk factors, and patterns of the microorganisms isolated in three ICUs. …”
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Circulating tumor DNA monitoring in advanced mutated melanoma (LIQUID-MEL) by Martines Gianmarco, Palazzi Carolina, Monica Gregorio, Verzè Michela, Pluchino Monica, Giudice Giulia Claire, Maffezzoli Michele, Mazzaschi Giulia, Manuguerra Roberta, Azzoni Cinzia, Bottarelli Lorena, Tiseo Marcello, Perrone Fabiana, Minari Roberta

“…The longitudinal analysis revealed intriguing patterns of ctDNA shedding in individual patients. …”
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Case Report: Acute polymorphic psychosis and NMDA-R IgG antibodies in serum: a follow-up case study by Katharina von Zedtwitz, Judith Weiser, Raphael J. Dressle, Simon J. Maier, Bernd Feige, Kathrin Nickel, Nils Venhoff, Katharina Domschke, Katharina Domschke, Joachim Brumberg, Sebastian Rauer, Ludger Tebartz van Elst, Luciana Hannibal, Harald Prüss, Harald Prüss, Alexander Rau, Dominique Endres

“…Serum NMDA-R antibodies were again detected with a titer of max. 1:320 using fixed-cell-based assays, but conventional magnetic resonance imaging (MRI), electroencephalography (EEG), and CSF findings were largely normal. …”
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Identification of WRKY gene family and analysis of drought stress expression in Poncirus polyandra by ZHANG Shanshan, RUAN Sunmei, YANG Wenzhong

“…Then, we screened out nine homologous drought resistance candidate genes of P. polyandra which had a high homology with P. trifoliata, such as PpWRKY8, PpWRKY12, PpWRKY13, PpWRKY19, PpWRKY23, PpWRKY27, PpWRKY28, PpWRKY30 and PpWRKY41. Their expression patterns under drought stress were detected by real-time fluorescence quantification, and the results revealed that all nine genes were significantly up-regulated under drought stress. …”
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Prevalence and antimicrobial resistance of Salmonella spp. isolated from swine and poultry farms in Cambodia by Bunna Chea, Sokom Kong, Sokha Thim, Naiheak Ban, Rithy Chrun, Vutey Venn, Cherry Fernandez-Colorado, Kroesna Kang

“…Multidrug resistance (MDR) patterns were identified in 91.30% (21/23) of poultry isolates and 52.63% (10/19) of swine isolates, with resistance spanning three to five antimicrobial classes. …”
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Protocol for Rhapsody: a longitudinal observational study examining the feasibility of speech phenotyping for remote assessment of neurodegenerative and psychiatric disorders by Allan H Young, Rebecca Strawbridge, Ammar Al-Chalabi, Dag Aarsland, Aleksandra Podlewska, Ray Chaudhuri, Elliot Hampsey, Marton Meszaros, Caroline Skirrow, Rosie H Taylor, Lazarus Chok, Jack Weston, Emil Fristed, Olabisi Awogbemila

“…New, remotely delivered diagnostic assessments that can detect early stage NPDs by profiling speech could enable earlier intervention and fewer missed diagnoses. …”
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Resting-State Brain Network Characteristics Related to Mild Cognitive Impairment: A Preliminary fNIRS Proof-of-Concept Study by Guohui Yang, Chenyu Fan, Haozheng Li, Yu Tong, Shuang Lin, Yashuo Feng, Fengzhi Liu, Chunrong Bao, Hongyu Xie, Yi Wu

“…Background: This study investigates the reliability of functional near-infrared spectroscopy (fNIRS) in detecting resting-state brain network characteristics in patients with mild cognitive impairment (MCI), focusing on static resting-state functional connectivity (sRSFC) and dynamic resting-state functional connectivity (dRSFC) patterns in MCI patients and healthy controls (HCs) without cognitive impairment. …”
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Impact of alcohol consumption on outcomes and potential of immune biomarkers for postoperative complications in trauma patients by Mohammad Majd Hammour, Yelda Anuk, Regina Breinbauer, Romina H. Aspera-Werz, Yuxuan Xin, Guanqiao Chen, Tina Histing, Sabrina Ehnert, Andreas K. Nüssler, Stefan Döbele

“…Acute systemic alcohol levels at the time of injury were not measured or considered in this study, as the focus was on chronic consumption patterns. Routine blood screening data were analyzed.ResultsExcept for CRP, blood values were comparable between patients with risky alcohol consumption and controls. …”
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Exploring the interaction effects of subclinical hypothyroidism and major depressive disorder on brain networks by Shuai Zhao, Jindan Wu, Xiaomei Liu, Yishan Du, Xiaoqin Wang, Yi Xia, Hao Sun, Haowen Zou, Xumiao Wang, Zhilu Chen, Rui Yan, Hao Tang, Qing Lu, Zhijian Yao

“…This study aimed was to investigate the neurological mechanisms underlying this co-occurrence using a data-driven approach to analyze brain activity patterns. Methods Twenty-nine patients diagnosed with MDD without any comorbid conditions (nSHypo-MDD) were included in the study, along with 29 MDD patients who also had SHypo (SHypo-MDD), 26 patients with SHypo only, and 29 healthy individuals as controls (HCs). …”
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Evaluation of Multidrug Resistance of <i>Salmonella</i> Isolated from Pork Meat Obtained from Traditional Slaughter Systems in Romania by Alexandra Tăbăran, Sorin Danel Dan, Liora Mihaela Colobaţiu, Marian Mihaiu, Sergiu Condor, Rodica Mărgăoan, Oana Lucia Crişan-Reget

“…This study investigates the prevalence and antibiotic resistance patterns of <i>Salmonella</i> serovars isolated from traditionally sourced pork meat in Romania. …”
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Antibody Preparation, Protein Expression, and Function Analysis of Cyp19a1b in Ovarian Differentiation in a Natural Triploid Teleost Qi River Crucian Carp (<i>Carassius auratus</i>... by Yongjing Li, Yujiao Jin, Limin Wu, Xuejun Li

“…In our previous study, we cloned the full length of the <i>cyp19a1b</i> gene from a natural triploid teleost Qi River crucian carp (<i>Carassius auratus</i>), andthe spatial and temporal expression patterns of <i>cyp19a1b</i> mRNA were detected. To further clarify the roles of <i>cyp19a1b</i> in the ovarian differentiation of Qi River crucian carp, we produced a polyclonal antibody of Cyp19a1b in this study. …”
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Examining the Eastern European extreme summer temperatures of 2023 from a long-term perspective: the role of natural variability vs. anthropogenic factors by M. Ionita, M. Ionita, P. Vaideanu, P. Vaideanu, B. Antonescu, B. Antonescu, B. Antonescu, C. Roibu, Q. Ma, V. Nagavciuc, V. Nagavciuc

“…Moreover, we highlight the intertwined impacts of climate change and multidecadal internal variability on HW patterns, with evidence suggesting that both contribute to the increasing frequency and intensity of these extreme events. …”
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Investigation of corrosion void formation in a Ni-based alloy under simulated MSW incineration condition by Xinxin Wu, Terrence Wenga, Wenchao Ma

“…The alloying elements had varied distribution patterns across the voids with Ni and W elements being immobile and mainly occurring in the base substrate while Cr and Al undergo selective removal and diffuse to the surface, leading to the formation of a porous Ni/W-enriched layer. …”
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Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy by Gregory B. Lesinski, Chrystal M. Paulos, Maggie Phillips, Jacklyn Hammons, Isaac Karpovsky, Zaid Mahdi, Natalie K. Horvat, Megan M. Wyatt, Margaret A. Hall, Cameron J. Herting, Richard A. Moffitt

“…In contrast, far more host immune cells infiltrate the KPC-Luc tumors, which have less stroma, although CD4+ and CD8+ T cells were similarly detected in the MT-5 tumors compared with KPC-GEMM in mice. …”
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Effects of Self-Assisted Manual Therapy Combined with a High-Intensity Walking Program on Musculoskeletal Pain, Functionality, and Posture in Older Adults: A Multicentre Randomized... by Gemma Victoria Espí-López, Laura Fuentes-Aparicio, Rocío Cogollos-de-la-Peña, Lucas Monzani, Elena Marques-Sule, Dagmar Pavlu, Anna Arnal-Gómez

“…These patterns of results remained stable at T2. The 5-times sit-to-stand test (<i>p</i> < 0.01) and the acromion position (<i>p</i> < 0.05) improved at T1 for the MTWG but not at T2. …”
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The interplay between brain and behavior during development: A multisite effort to generate and share simulated datasets by Neda Sadeghi, Isabelle F. van der Velpen, Bradley T. Baker, Ishaan Batta, Kyle J. Cahill, Sarah Genon, Ethan McCormick, Léa C. Michel, Dustin Moraczewski, Masoud Seraji, Philip Shaw, Rogers F. Silva, Najme Soleimani, Emma Sprooten, Øystein Sørensen, Adam G. Thomas, Audrey Thurm, Zi-Xuan Zhou, Vince D. Calhoun, Rogier Kievit, Anna Plachti, Xi-Nian Zuo, Tonya White

“…These datasets and code that were used to generate the datasets can be downloaded and used by the research community to apply different longitudinal models to determine the underlying patterns and assumptions where the ground truth is known.…”
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