Bio-induced overproduction of heterocycloanthracin-like bacteriocin in Lysinibacillus macroides by Aspergillus austroafricanus: optimization of medium conditions and evaluation of... by Philomena Edet, Maurice Ekpenyong, Atim Asitok, David Ubi, Cecilia Echa, Uwamere Edeghor, Sylvester Antai

“…The RSM model predictions were optimized, validated, and scaled up in a 5-L bioreactor via submerged batch fermentation. The BAC was extracted with ethyl acetate, purified via silica gel column chromatography, and identified via semipreparative high-performance liquid chromatography using bioactive standards with known retention times. …”
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Impact of adding eicosapentaenoic and docosahexaenoic acid-rich fish oil in sow and piglet diets on blood oxylipins and immune indicators of weaned piglets by E. Llauradó-Calero, I. Badiola, I. Samarra, R. Lizardo, D. Torrallardona, E. Esteve-Garcia, N. Tous

“…At the end of the trial, blood was collected from piglets in the first batch (n = 48). Serum fatty acids (FAs) were quantified by GC, plasma oxylipins by ultra-HPLC-MS, and plasma immune indicators by ELISA. …”
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Rapid Fine-Grained Damage Assessment of Buildings on a Large Scale: A Case Study of the February 2023 Earthquake in Turkey by Zhonghua Hong, Hongyang Zhang, Xiaohua Tong, Shijie Liu, Ruyan Zhou, Haiyan Pan, Yun Zhang, Yanling Han, Jing Wang, Shuhu Yang

“…Subsequently, we deploy an automated workflow for batch processing and registration of open-source building footprints, enabling the accurate extraction of building height changes from dual-time DSMs. …”
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Organoid in droplet: Production of uniform pancreatic cancer organoids from single cells by Haitao Liu, Tingting Tao, Zhongqiao Gan, Yingying Xie, Yaqing Wang, Yizhao Yang, Xu Zhang, Xianliang Li, Jianhua Qin

“…This high-throughput method can produce thousands of organoids in a single batch. The droplets can serve as templates for synthesizing defined microgels with proper stiffness similar to that of native tumors, facilitating functional expressions of PCOs. …”
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Breaking Digital Health Barriers Through a Large Language Model–Based Tool for Automated Observational Medical Outcomes Partnership Mapping: Development and Validation Study by Meredith CB Adams, Matthew L Perkins, Cody Hudson, Vithal Madhira, Oguz Akbilgic, Da Ma, Robert W Hurley, Umit Topaloglu

“…The implementation successfully processes diverse data types through both individual term mapping and batch processing capabilities. ConclusionsOur validated large language model–based tool effectively automates the transformation of clinical data into the OMOP format while maintaining high accuracy. …”
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Microchip gas chromatographic columns dedicated for space exploration: Stationary phase coating, setup optimization and evaluation of column performances by Arnaud Philippart, Valérie Peulon-Agasse, Malak Rizk-Bigourd, Audrey Boco-Simon, Gabin Bergerot, Guillaume Rioland, Arnaud Buch, Cyril Szopa, Pascal Cardinael

“…Stationary phase films of different thicknesses, from 0.032 to 0.260 µm, were coated on different microchips of the same production batch to assess the influence of film thickness on the chromatographic performances. …”
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Amniotic fluid-derived stem cells: potential factories of natural and mimetic strategies for congenital malformations by Cristiane S. R. Fonteles, Julia Enterria-Rosales, Ying Lin, John W. Steele, Ramiro A. Villarreal-Leal, Jing Xiao, Daniel I. Idowu, Beck Burgelin, Bogdan J. Wlodarczyk, Richard H. Finnell, Bruna Corradetti

“…A physicochemical, structural, and molecular comparison was conducted with exosomes (EXOs) released from the same batch of cells. Additionally, their distribution patterns in female mice were evaluated following in vivo administration, along with an assessment of their safety profile throughout gestation in a mouse strain predisposed to neural tube defects (NTDs). …”
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Simultaneous adsorptive removal of Pb2+, Cd2+, Cu2+, and Zn2+ using raw Norway Spruce biomass: a low-cost and eco-friendly solution for wastewater treatment by Ibrahim G. Al-Labadi, Márk Horváth, Ayah T. Alkilani, Alaa M. Al-Ma'abreh, Mohammed J. K. Bashir, Basem E. Keshta, Basem E. Keshta, Ghadir Hanbali, Wail Al Zoubi, Mostafa R. Abukhadra, Mostafa R. Abukhadra, Haifa A. Alqhtani, Mohamed Hamdy Eid, Mohamed Hamdy Eid

“…This study evaluated the unmodified Norway Spruce Wood Residue (NSWR), an abundant lignocellulosic biomass, for the simultaneous removal of Pb2+, Cd2+, Zn2+, and Cu2+ from a quaternary aqueous system. A series of batch adsorption experiments were performed to assess the influence of key operational parameters (pH, contact time, adsorbent dose, temperature, particle size, initial concentration), with equilibrium data subsequently fitted to Langmuir and Freundlich isotherm models, and the NSWR characterized using FTIR and EDS analyses. …”
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Solid-State Kinetic Modeling and Experimental Validation of Cu-Fe Bimetallic Catalyst Synthesis and Its Application to Furfural Hydrogenation by Bárbara Jazmín Lino Galarza, Javier Rivera De la Rosa, Eduardo Maximino Sánchez Cervantes, Carlos J. Lucio-Ortiz, Marco Antonio Garza-Navarro, Carolina Solís Maldonado, Ramón Moreno-Tost, Juan Antonio Cecilia-Buenestado, Antonia Infantes Molina

“…This interconnects with the initial section of the study, in which we found that active Cu₄Fe sites have superior performance in the CuFe/Al₂O₃ catalyst in the hydrogenation batch test.…”
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Sesbania grandiflora activated carbon as a high-efficiency stable and reusable adsorbent for sustainable fluoride removal by D. Sivakumar, M. Perarul Selvan, J. Nouri, M. Juhi Jabin, S. Priyanka, G. Madhubala

“…This study evaluates Sesbania grandiflora activated carbon as a stable, reusable fluoride adsorbent, focusing on its characterization, optimal adsorption conditions, and reusability.METHODS: Sesbania grandiflora activated carbon was synthesized and characterized using fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive x-ray spectroscopy, and x-ray diffraction to assess structural integrity and surface interactions. Batch adsorption experiments were conducted by varying particle size (up to 2.360 millimeter), dosage (2–10 grams), agitation speed (100–500 revolution per minutes), and contact time (up to 140 minutes) to determine the optimal conditions for fluoride removal. …”
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Effects of cloprostenol sodium and carbetocin on synchronous parturition and colostrum composition in large white sows by Hongmei Gao, Miaolian Peng, Rongzhi Zhong, Zhenhua Xue, Zhenqiang Liu, Shiqiao Weng, Longchao Zhang, Dong Wang, Yan Liu, Jianhui Tian, Lixian Wang

“…Abstract Background Synchronized parturition is essential for optimizing batch production and implementing effective "all-in/all-out" management strategies. …”
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Recent progress on functional hydrogels as biomaterial for biomedicine by Hepeng Yang, Taotao Zeng, Tao Jiang, Qin Wei, Seyed Dariush Taherzade, Xiaolan Peng, Junping Zhu, Zeyan Zhou

“…Although natural polymer hydrogels (such as collagen and Matrigel) have good biological activity, they have problems of batch differences and insufficient mechanical properties; synthetic polymer hydrogels (such as polyethylene glycol) can achieve controllable performance through chemical modification, but their biological inertia needs to be optimized through compound strategies. …”
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Development of a cELISA for effective detection of the antibody against H7 subtype of avian influenza virus by Cong-cong WANG, Si-wen WANG, Ying ZHANG, Jian-zhong SHI, Xin YIN, Cheng-jun LI, Xiu-rong WANG

“…And the repeatability experiment revealed that the coefficients of variation (CV) of intra- and inter-batch repetition were all less than 12%. The data indicated that the cELISA antibody-detection method established in this study provided a simple and accurate technical support for the detection of a large number of antibody samples of H7-AIV.…”
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Safety Profile of Intravenous Ferulic Acid Nanoparticles: Acute Toxicity and Neurological Effects in Sprague-Dawley Rats by Huang H, Xuan Y, Ma ZC

“…Acute toxicity, pharmacokinetics, and network pharmacology analysis were conducted to assess safety profiles and potential mechanisms.Results: FA-LNPs were successfully prepared using an optimized active loading method, achieving high drug loading (≥ 4 mg/mL), superior encapsulation efficiency (EE%) ≥ 80%, and uniform particle size distribution (< 200 nm, PDI=0.053), zeta potential of +5.97 mV (Quality Factor = 1.701), excellent storage stability over two weeks, and was scaled up for batch production. The Morris water maze test revealed an effective FA concentration of 50 mg/kg, with FA-LNPs achieving 46.5 mg/kg through active loading method. …”
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In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adu... by Evangelia N. Kerezoudi, Marigoula Vlassopoulou, Evdokia K. Mitsou, Georgia Saxami, Georgios Koutrotsios, Ioanna Taflampa, Konstantinos C. Mountzouris, Ignacio Rangel, Robert J. Brummer, Georgios I. Zervakis, Vasiliki Pletsa, Panagiotis Georgiadis, Adamantini Kyriacou

“…This study assessed in vitro the prebiotic-like effects of multiple forms of this mushroom. An in vitro static batch fermentation was performed for 24 h with faecal inocula from five apparently healthy older adults in the presence of the following PE forms: whole food matrix (PEWS), in vitro digested (PEWSD) and rich in β-glucans extract (PEWSE). …”
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Modelling of acid brown 14 and acid yellow 36 dyes adsorption from water by self-nitrogen-doped activated carbon by Mohamed A. El-Nemr, Mohamed A. Hassaan, Murat Yılmaz, Ahmed El Nemr

“…The AC7-800 adsorbent was successfully tested and approved to eliminate colours from water in batch trials. The AC7-800 samples were analyzed using BET, SEM, EDX, XRD, FTIR, TGA, and DTA techniques. …”
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Similarities between Ixodes ricinus and Ixodes inopinatus genomes and horizontal gene transfer from their endosymbionts by Valérie O. Baede, Oumayma Jlassi, Paulina M. Lesiczka, Hend Younsi, Hans J. Jansen, Khalil Dachraoui, Jane Segobola, Mourad Ben Said, Wouter J. Veneman, Ron P. Dirks, Hein Sprong, Elyes Zhioua

“…Subtraction of the genome assemblies identified the presence of some unique sequences, which could not be confirmed when screening a larger batch of I. ricinus and I. inopinatus ticks using PCR. …”
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Alternative splicing for tuneable expression of protein subunits at desired ratios by Christel Aebischer-Gumy, Pierre Moretti, Timothee Brunstein Laplace, Jana Frank, Ysaline Grand, Farid Mosbaoui, Emilie Hily, Anna Galea, Megane Peltret, Carole Estoppey, Daniel Ayoub, Roberto Giovannini, Martin Bertschinger

“…To illustrate this approach, we developed constructs expressing varying ratios of GFP and dsRED and extended their application to multimeric proteins such as monoclonal antibodies, achieving industrially relevant expression levels (>1 g/L) in a 14-day fed-batch process. The stability of the splice ratio was confirmed by droplet digital PCR in a stable pool cultivated over a 28-day period, while product quality was assessed via intact mass analysis, demonstrating absence of product-related impurities resulting from undesired splice events. …”
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Bioinspired Spider Silk Fiber of MOF‐Based Zwitterionic Hydrogel for Low‐Humidity Atmospheric Water Harvesting by Hengyu Pan, Lingmei Zhu, Huijie Wei, Chang Gao, Maolin Zhou, Tiance Zhang, Qiang Luo, Boyang Tian, Jianhua Wang, Yongping Hou, Yongmei Zheng

“…The temperature of a single PCLC knot can rise to 46.4 °C for ≈3600 s, and the temperature of a batch of PCLC spider knot fibers can rise to 112.1 °C for ≈3600 s (under ≈1 sun illumination), which has excellent photothermal effect. …”
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Synergistic transcriptomic and metabolomic analyses in Zi geese ovaries with different clutch lengths by Shengjun Liu, Jiaxin Yin, Kexin Cong, Shan Yue, Yuanliang Zhang, Jinyan Sun, Xiaofang Ren, Ke Jiang, Yunuo Liu, Xiuhua Zhao

“…In the first experiment, 200 Zi geese were selected, all 230 days old, were selected from the same batch and raised individually in the same environment. …”
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