A CRISPR/Cas9-based system using dual-sgRNAs for efficient gene deletion in Mycobacterium abscessus by Linai Li, Yuxiang Hu, Dan Wang, Xin Li, Shengjuan Bao, Taibing Deng, Qinglan Wang

“…Furthermore, we systematically optimized the modular arrangement of genetic components in Cas9/dual-sgRNA expression plasmids and refined their construction workflow, achieving a significant reduction in cassette loss rates while enabling single-step plasmid assembly. …”
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Modern solutions for maintaining of skin pH with dermatocosmetic care products by N. V. Zilberberg, A. I. Polishchuk

“…Results of assessment of morphofunctional and clinical state of skin (hydration level, dryness, transepidermal water loss), demonstrate the beneficial effects of using the line of specialized cosmetic products with pH 5.0 to restore the protective function of skin and maintain an optimal level of skin hydration.…”
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Hospital length of stay and its predictors among surgical patients at public hospitals in Addis Ababa, Ethiopia by Samuel Dessu Sifer, Abduselam Ahmed Abdela, Milkiyas Solomon Getachew, Redait Awoke Assefa, Abatalem Minlargeh Abere

“…Comorbidity (AHR: 3.32; 95% CI: 1.01, 10.85), infection (AHR: 3.14; 95% CI: 1.77, 12.72), weight loss (AHR: 1.62; 95% CI: 1.03, 2.56), change in dietary pattern (AHR: 1.52; 95% CI; 1.98, 2.35) and change in functional capacity (AHR: 1.53; 95% CI: 1.12, 2.13) were independent predictors of length of hospital stay.Conclusion and recommendationsOur findings indicate that prolonged hospitalization is often a consequence of delayed recovery from surgery, particularly among patients with complicating conditions such as comorbidities, infections, poor nutritional status, and limited functional capacity. …”
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Wild ActionFormer: Enhancing wildlife action recognition for 11 endangered species in Wolong by Dan Liu, Qianqian Sun, Jin Hou, Bochuan Zheng, Jindong Zhang, Desheng Li, Tomas Norton, Jifeng Ning

“…The introduction of the Focal Loss reweighting strategy optimizes the loss for long-tail classes, mitigating the bias towards head data. …”
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ScarNet: Development and Validation of a Novel Deep CNN Model for Acne Scar Classification With a New Dataset by Masum Shah Junayed, Md Baharul Islam, Afsana Ahsan Jeny, Arezoo Sadeghzadeh, Topu Biswas, A. F. M. Shahen Shah

“…The pre-processed input images are fed into our proposed model, namely <italic>ScarNet</italic>, for deep feature map extraction. The optimizer, loss function, activation functions, filter and kernel sizes, regularization methods, and the batch size of the proposed architecture are tuned so that the classification performance is maximized while minimizing the computational cost. …”
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Case Report: Diagnostic assessment, developmental trajectory and treatment approaches in a case of a complex neurodevelopmental syndrome associated with non- synonymous variants in... by V. Napoli, V. Napoli, S. Guerrera, F. Demaria, G. Piccolo, G. Piccolo, A. Cianfa, S. Passarini, S. Passarini, M. G. Logrieco, G. Zanni, G. Valeri, S. Vicari, S. Vicari

“…Although Rett-like features are mainly due to MECP2 loss-of-function, MECP2 deficiency disrupts GABAergic signaling, making GABA modulators potential therapeutic targets. …”
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Evaluation of anhydrous processing and storage methods of the temperate bacteriophage ɸV10 for integration into foodborne pathogen detection methodologies. by Andrew Kanach, Theresa Bottorff, Min Zhao, Jun Wang, George T C Chiu, Bruce Applegate

“…Applying phage to dissolvable paper yielded key attributes to consider when addressing phage viability, however, optimized methodology still resulted in an approximate five-log reduction in titer of viable phage. …”
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Assessing the Influence of Selected Permeabilization Methods on Lymphocyte Single-Cell Multi-Omics by Shifan Ding, Na Lu, Hassan Abolhassani

“…If the measurement of combined surface and intracellular markers is required to be achieved, the modified fixation and permeabilization method is recommended because of a lower transcriptomic loss and more precise proteomic fingerprint detected. (4) Conclusions: The findings of this study support the potential possibility for integrating intracellular proteomics, which needs to be optimized and tested with newly designed oligonucleotide-tagged antibodies targeting intracellular proteins.…”
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Fabrication of a poly(m‑aminophenol)/3-aminopropyl triethoxysilane/graphene oxide ternary nanocomposite for removal of Cu(II) from aqueous solution by Abeer S. Elsherbiny, Mohamed E. Elhalwagy, Ali H. Gemeay

“…The adsorption of Cu(II) on this composite was optimized under various experimental conditions. Furthermore, the isotherm data of the uptake of Cu(II) on PmAP/APTES/GO(6.6) were found to agree with the Freundlich and Langmuir model’s linear and nonlinear forms. …”
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Threshold Effects, Sacrifice Ratio and Government Debt The Smooth Transition Regression Approach by sara ghobadi

“…This ratio is calculated as follows: SR=S=0T∂yt+s∂εm1∂t+T∂εmt                                                                                                      (2) Itt : Inflation targeting is defined as an optimal monetary policy. The optimal monetary policy is obtained by minimizing the social loss function according to the constraints of the monetary transmission mechanism. …”
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Comparison of surgical outcomes and postoperative nutritional parameters between subtotal and proximal gastrectomy in patients with proximal early gastric cancer by Wataru Soneda, Masanori Terashima, Yusuke Koseki, Kenichiro Furukawa, Keiichi Fujiya, Yutaka Tanizawa, Hiroya Takeuchi, Etsuro Bando

“…Conclusion sTG is the preferred function‐preserving gastrectomy procedure for early gastric cancer in the upper third of the stomach because it is associated with less postoperative body weight loss and psoas mass index loss.…”
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Pneumothorax detection and segmentation from chest X-ray radiographs using a patch-based fully convolutional encoder-decoder network by Jakov Ivan S. Dumbrique, Jakov Ivan S. Dumbrique, Reynan B. Hernandez, Reynan B. Hernandez, Juan Miguel L. Cruz, Ryan M. Pagdanganan, Prospero C. Naval

“…Ablation studies further reveal that using a mixed Tversky and Focal loss function significantly improves performance compared to using solely the Tversky loss. …”
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Research Progress of Solvent-free Lipase Catalysis and Its Application in the Food Field by Jiaying XIN, Qi SONG, Jingyi XU, Weidan ZHANG, Jiao HE, Chungu XIA

“…Lipase is one of the important hydrolase in the food industry, which is widely used in many fields such as production of flavor ester, development of functional ester and the modification of oil and fat. …”
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Lightweight coal mine conveyor belt foreign object detection based on improved Yolov8n by Jierui Ling, Zhibo Fu, Xinpeng Yuan

“…Lastly, the original CIoU loss function in Yolov8n is replaced with MPDIoU, which allows the model to more accurately predict the position and shape of bounding boxes in the object detection task, thereby further reducing accuracy loss. …”
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Downscaling, bias correction, and spatial adjustment of extreme tropical cyclone rainfall in ERA5 using deep learning by Guido Ascenso, Andrea Ficchì, Matteo Giuliani, Enrico Scoccimarro, Andrea Castelletti

“…The key novelty of RA-Ucmpd is its loss function, the compound loss, which optimizes both a pixel-wise bias metric (the MSE) and a spatial verification metric (a modified version of the Fractions Skill Score). …”
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YOLO-RDM: Innovative Detection Methods for Eggplants and Stems in Complex Natural Environment by Qin Liu, Zhibin Zhou, Lu Xiong, Meilian Lu, Jingwen Ouyang

“…Next, the DD-Head structure reparameterization detection head module is integrated, effectively merging deep and shallow information while retaining small-scale target feature information to improve detection accuracy. Finally, the loss function is substituted with the MPDIoU loss, optimizing the bounding box regression algorithm and enhancing convergence speed and regression accuracy. …”
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Multi-Scale Spatial Perception Attention Network for Few-Shot Hyperspectral Image Classification by Yang Li, Jian Luo, Haoyu Long, Qianqian Jin

“…In the decoder, adaptive residual aggregation (ARA) is proposed to embed high-level semantic information into low-level features using a residual structure, thereby enhancing the perception of contextual information. A weighted CL-MixedLoss function (CL-MixedLoss) is proposed to solve the problem of imbalanced heterogeneous pixels in HSIs. …”
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A Deep Learning Framework for Chronic Kidney Disease stage classification by Gayathri Hegde M, P Deepa Shenoy, Venugopal KR, Arvind Canchi

“…The models were assessed using performance metrics such as accuracy, precision, recall, F1-Score, Loss, Validation Loss and computation time. The CNN model outperformed others, achieving an accuracy between 98%-99.5% for all FS methods. …”
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Microsurgery Techniques for Patients with Traumatic Thumb Amputation by M. M. Valeev, I. Z. Garapov, E. M. Biktasheva

“…The reconstruction of functional abilities of the hand with traumatic thumb amputation must be carried out taking into account the available methods of surgical correction and which one of these would be optimal, the evaluation of the patient’s mental status and his or her drive for the fastest possible recovery of the shape and function of the hand. …”
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Advancing carbon capture with bio-inspired membrane materials: A review by W. Rahmah, K. Khoiruddin, I.G. Wenten, S. Kawi

“…These challenges underscore the need for continued research to optimize membrane design and functionality, ensuring their viability for large-scale implementation. …”
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