Construction of a reading frame–independent yeast two-hybrid vector system for site-specific recombinational cloning and protein interaction screening by Richard H. Maier, Christina J. Brandner, Helmut Hintner, Johann W. Bauer, Kamil Önder

“…Since the Y2H system per se is a positive selection system, only pairs of bait and prey genes harboring the correct reading frames will emerge. We tested the new vectors within the Y2H system and demonstrated full functionality without any undesired effects on the Y2H system itself. …”
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Microglia in the aged brain develop a hypoactive molecular phenotype after surgery by Zhuoran Yin, Anna K. Leonard, Carl M. Porto, Zhongcong Xie, Sebastian Silveira, Deborah J. Culley, Oleg Butovsky, Gregory Crosby

“…However, they can also contribute to neurodegeneration through their pro-inflammatory properties and phagocytic functions. Acute post-operative cognitive deficits have been associated with inflammation, and microglia have been implicated primarily based on morphological changes. …”
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The impact of health care financing on the economic growth: EU countries analysis by Anastasiia Samoilikova, Rosen Kunev

“…The nature of the analysed indices distribution was estimated based on results of Shapiro-Wilk test. So, Pearson or Spearman correlation coefficient was chosen. …”
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Effects of Potassium Supply in Nutrient Solution on Water and Nutrient Absorption of Substrate-Grown Tomato Plants by Jinxiu Song, Rong Zhang, Bingyan Fu, He Chen, Xiaoming Song, Gaoqiang Lv, Rongqiang Zhang

“…Potassium (K⁺) functions as a critical “regulator” and “quality element” in plants, with its physiological roles varying across developmental stages. …”
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The impact of urbanization on land use land cover change using geographic information system and remote sensing: a case of Mizan Aman City Southwest Ethiopia by Addis Bikis, Muluye Engdaw, Digvijay Pandey, Binay Kumar Pandey

“…Deforestation for urban functions, including timber production, construction materials, and firewood, adversely affects the environment. …”
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Knockdown of nrf2 Exacerbates TNF-α-Induced Proliferation and Invasion of Rheumatoid Arthritis Fibroblast-Like Synoviocytes through Activating JNK Pathway by Yu Du, Qian Wang, Na Tian, Meng Lu, Xian-Long Zhang, Sheng-Ming Dai

“…In this study, we aimed to explore the expression and upstream regulatory factors of nrf2 and revealed its functions in modulating the proliferation and invasion in RA-FLS. …”
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Effects of hepatitis B virus infection and antiviral therapy on the clinical prognosis of nasopharyngeal carcinoma by Jing‐Jin Weng, Jia‐Zhang Wei, Min Li, Jin‐Long Lu, Yang‐Da Qin, He Jiang, Shen‐Hong Qu

“…Conclusions Hepatitis B virus infection is an independent risk factor for early stage NPC, which may be associated with its reduced immune functions compared to the HBsAg(−) group. Anti‐HBV treatment may improve the prognosis of HBV‐infected NPC patients.…”
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Puma algorithm for environmental emissions and generation costs minimization dispatch in power systems by Badr Al Faiya, Ghareeb Moustafa, Hashim Alnami, Ahmed R. Ginidi, Abdullah M. Shaheen

“…This study adopts a more complex approach by formulating the fuel cost and produced emission objectives as cubic polynomial functions. Also, this paper proposes a novel Puma Optimization Operator (POO), inspired by the hunting behavior of pumas for the optimal allocation of power generation across various units, considering both economic and environmental factors. …”
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Arsenic Trioxide Enhances the Efficacy of PD‐1 Inhibitors in Hepatocellular Carcinoma by Inducing Immunogenic Cell Death via the ROS/ERS Pathway by Xionghui Wang, Simo Cheng, Yannan Xu, Tianxiao Zheng, Changquan Ling, Juan Du

“…ATO‐treated HCC cells exhibited enhanced immunogenicity, functioning as prophylactic and therapeutic vaccines to stimulate antitumor immunity. …”
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Necrotic and Inflammatory Changes on Acute Studies of Siam Leaf (Chromolaena Odorata) Alcoholic Extract on Lead Induced Toxicity of Cardio-Pulmonary Morphology of Adult Albino Wist... by Ilegbedion, I.G.1*, Beredugo, S.,2 Aturaka, O.S.3, Tabowei, W.T.4.

“…Long-term exposure of adults can result in decreased performance in some tests of cognitive performance that measure functions of the nervous Cardio-pulmonary system.  …”
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MASP1 in stomach adenocarcinoma: linking diagnosis, prognosis, and tumor immunity by Chuyu Xiao, Fuyang Hong, Guanzi Chen, Wenli Xu, Yusheng Jie

“…Enrichment analyses were conducted to elucidate the functions and pathways associated with MASP1. The relationship between MASP1 expression and tumor immune infiltration was analyzed using single-sample Gene Set Enrichment Analysis (ssGSEA), Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT), Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE), and Spearman correlation methods. …”
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Transcriptomic analysis of non-leukemic cell subsets in azacytidine-responsive AML highlights pathways associated with adhesion, platelet aggregation, and angiogenesis in mice and... by Nancy D. Ebelt, Suvithanandhini Loganathan, Lara C. Avsharian, Edwin R. Manuel

“…Transcriptional analysis of AZA-treated samples revealed decreased cell type scores for suppressive subsets and increased pathway scores for T and B cell functions. Comparisons of gene ontology annotations enriched from genes differentially regulated by AZA in human and mouse blood samples revealed overlap in numerous biological pathways including adhesion, thrombosis, and angiogenesis. …”
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Morphometrics and Growth Patterns of Squids in the North and South Coasts of Java, Indonesia by Ayu Ervinia, Charles P H Simanjuntak, Sulistiono Sulistiono, Dwi Yuni Wulandari, Zairion Zairion

“…Abstract Squids play vital ecological and economic functions as keystone species in marine food webs and integral components of global capture fisheries. …”
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Co-Designed Mobile-Based Cognitive Training for Older Chinese Americans: Protocol for a Pilot Randomized Controlled Trial Assessing Feasibility and Acceptability by Tingzhong Xue, Aybey Amy Wei, Bei Wu, Camilla Sanders, Eleanor Schildwachter McConnell, Hanzhang Xu

“…Global cognition, mental health, physical functioning, and quality of life will be assessed at baseline, 8, and 12 weeks. …”
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Metabolomic-Based Assessment of Earthworm (<i>Eisenia fetida</i>) Exposure to Different Petroleum Fractions in Soils by Meiyu Liu, Mutian Wang, Xiaowen Fu, Fanyong Song, Fangyuan Zhou, Tianyuan Li, Jianing Wang

“…However, the contributions of other fractions to overall toxicity should not be ignored, as each fraction uniquely affects key metabolic pathways and biological functions. These findings emphasize the importance of monitoring metabolic perturbations caused by petroleum components in non-target organisms such as earthworms. …”
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Genetic determinants of circulating sphingolipid concentrations in European populations. by Andrew A Hicks, Peter P Pramstaller, Asa Johansson, Veronique Vitart, Igor Rudan, Peter Ugocsai, Yurii Aulchenko, Christopher S Franklin, Gerhard Liebisch, Jeanette Erdmann, Inger Jonasson, Irina V Zorkoltseva, Cristian Pattaro, Caroline Hayward, Aaron Isaacs, Christian Hengstenberg, Susan Campbell, Carsten Gnewuch, A Cecilej W Janssens, Anatoly V Kirichenko, Inke R König, Fabio Marroni, Ozren Polasek, Ayse Demirkan, Ivana Kolcic, Christine Schwienbacher, Wilmar Igl, Zrinka Biloglav, Jacqueline C M Witteman, Irene Pichler, Ghazal Zaboli, Tatiana I Axenovich, Annette Peters, Stefan Schreiber, H-Erich Wichmann, Heribert Schunkert, Nick Hastie, Ben A Oostra, Sarah H Wild, Thomas Meitinger, Ulf Gyllensten, Cornelia M van Duijn, James F Wilson, Alan Wright, Gerd Schmitz, Harry Campbell

“…The strongest associations were observed in or near 7 genes functionally involved in ceramide biosynthesis and trafficking: SPTLC3, LASS4, SGPP1, ATP10D, and FADS1-3. …”
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Transcranial Doppler sonography follow-up study in mild vascular cognitive impairment. by Mariagiovanna Cantone, Manuela Pennisi, Giuseppe Lanza, Raffaele Ferri, Francesco Fisicaro, Francesco Cappellani, Emanuele David, Vito Nicosia, Klizia Cortese, Giovanni Pennisi, Valentina Puglisi, Rita Bella

“…Namely, the Montreal Cognitive Assessment (MoCA), the 17-items Hamilton Depression Rating Scale (HDRS), and the Stroop Color-Word Interference Test (StroopT) were administered to screen for global cognitive status, to quantify depressive symptoms, and to explore executive functions, respectively. …”
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Effectiveness and cost-effectiveness of a web-based routine assessment with integrated recommendations for action for depression and anxiety (RehaCAT+): protocol for a cluster rand... by Reinhold Kilian, Harald Baumeister, Yannik Terhorst, Johannes Knauer, Paula Philippi, Selina Kallinger, Sandro Eiler, Tamara Waldmann, Morten Moshagen, Martina Bader

“…Secondary outcomes are depression at discharge (T1) and 6 months follow-up (T2) as well as anxiety, satisfaction with participation in social roles and activities, pain impairment, fatigue, sleep, health-related quality of life, self-efficacy, physical functioning, alcohol, personality and health economic-specific general quality of life and socioeconomic cost and benefits at T1-3. …”
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Danggui Shaoyao San Alleviates Early Cognitive Impairment in Alzheimer's Disease Mice Through IRS1/GSK3β/Wnt3a‐β‐Catenin Pathway by Kai‐Xin Zhang, Ning Sheng, Peng‐Li Ding, Ji‐Wei Zhang, Xiang‐Qing Xu, Ya‐Han Wang

“…We explored the potential mechanism by which Danggui Shaoyao San (DSS) modulates central glucose metabolism via the insulin receptor substrate 1 (IRS1)/glycogen synthase kinase‐3β (GSK3β)/Wnt3a‐β‐catenin pathway, thereby exerting protective effects on cognitive functions. Methods In vitro, HT22 cells were induced with streptozotocin (STZ) to investigate the impact of GSK3β on pathway transduction. …”
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Genetic model of motor neuron disease in B6SJL-Tg mice: new data on the dynamics of motor symptoms and immunohistochemical manifestations of the neurodegenerative process by А. V. Stavrovskaya, D. N. Voronkov, E. A. Artyomova, B. V. Belugin, М. М. Shmarov, N. G. Yamshchikova, А. S. Gushchina, А. S. Olshansky, B. S. Naroditskiy, S. N. Illarioshkin

“…Aim of study. To assess motor functions, dynamics of survival, and morphological changes in the spinal cord of transgenic B6SJL-Tg (SOD1*G93A) mice. …”
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