Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region by Kai Schlepckow, Kathryn M Monroe, Gernot Kleinberger, Ludovico Cantuti‐Castelvetri, Samira Parhizkar, Dan Xia, Michael Willem, Georg Werner, Nadine Pettkus, Bettina Brunner, Alice Sülzen, Brigitte Nuscher, Heike Hampel, Xianyuan Xiang, Regina Feederle, Sabina Tahirovic, Joshua I Park, Rachel Prorok, Cathal Mahon, Chun‐Chi Liang, Ju Shi, Do Jin Kim, Hanna Sabelström, Fen Huang, Gilbert Di Paolo, Mikael Simons, Joseph W Lewcock, Christian Haass

“…Moreover, in a mouse model for Alzheimer's disease‐related pathology, 4D9 reduced amyloidogenesis, enhanced microglial TREM2 expression, and reduced a homeostatic marker, suggesting a protective function by driving microglia toward a disease‐associated state.…”
Get full text

Analysis of the prognostic efficacy of syndecan-1 for patients with ACLF and its functional role in liver regeneration by Yingli He, Xiaoli Zhang, Yichen Yao, Juan Li, Shan Fu, Yali Feng, Tianzhi Ni, Ruojing Wang, Qiao Zhang, Yushan Liu, Zhijun Liu, Jinfeng Liu, Yuan Yang, Yingren Zhao, Yalei Zhao

Get full text

DNMT1-mediated regulation of somatostatin-positive interneuron migration impacts cortical architecture and function by Julia Reichard, Philip Wolff, Song Xie, Ke Zuo, Camila L. Fullio, Jian Du, Severin Graff, Jenice Linde, Can Bora Yildiz, Georg Pitschelatow, Gerion Nabbefeld, Lilli Dorp, Johanna Vollmer, Linda Biemans, Shirley Kempf, Minali Singh, K. Naga Mohan, Chao-Chung Kuo, Tanja Vogel, Paolo Carloni, Simon Musall, Geraldine Zimmer-Bensch

“…Abstract The coordinated development of cortical circuits composed of excitatory and inhibitory neurons is critical for proper brain function, and disruptions are linked to a spectrum of neuropsychiatric disorders. …”
Get full text

Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages. by Bartłomiej Taciak, Maciej Białasek, Agata Braniewska, Zuzanna Sas, Paulina Sawicka, Łukasz Kiraga, Tomasz Rygiel, Magdalena Król

“…We found out that the phenotype (expression of particular macrophage-characteristic genes and surface markers) and functional characteristics (phagocytosis and NO production) of RAW 264.7 cell line remains stable through passages: from passage no. 10 up to passage no. 30. …”
Get full text

March5-mediated Trim28 degradation preserves islet β-cell function in mice by Yangshan Chen, Wei Pang, Guixing Ma, Yongcong Yan, Zhiyu Xiao, Yong Chen, Zhen Ding, Litong Chen, Xiaoting Hou, Huiling Cao

“…However, the regulatory pathways underlying β-cell function remain incompletely understood. Here, we identify that March5 and Trim28 as key modulators of β-cell function. …”
Get full text

Structural and Functional Reorganization of the Sensorimotor Cortex During Ligation of the Common Carotid Arteries (Experimental Study) by L. M. Makarieva, V. A. Akulinin, M. S. Korzhuk, S. S. Stepanov, A. Y. Shoronova, D. B. Avdeev, I. G. Tsuskman

“…To explore the structural and functional changes of neurons, glial cells, and synaptic terminals in layers I, III, and V of the sensorimotor cortex (SMC) of the rat brain after bilateral common carotid artery ligation (CCAL).Material and methods. …”
Get full text

Integration and functionality of human iPSC-derived microglia in a chimeric mouse retinal model by Chun Tang, Qi-Qi Zhou, Xiu-Feng Huang, Ya-Yi Ju, Bi-Lin Rao, Zhi-Cong Liu, Yi-An Jia, Zhan-Pei Bai, Qing-Yang Lin, Lin Liu, Jia Qu, Jun Zhang, Mei-Ling Gao

“…Our approach achieved over 86% integration of human microglia, which maintained key functions including proliferation, immune responsiveness, and synaptic pruning over a 14-day culture period. scRNA-seq of xMG revealed a shift in microglial signatures compared to monoculture iMG, indicating a transition to a more in vivo-like phenotype. …”
Get full text

Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth by Gaoxiang Zhao, Shudi Luo, Hong Zhao, Qingxia Ma, Hongfei Jiang, Lin Wang, Juanjuan Liu, Dong Guo, Runze Wang, Qianqian Xu, Jie Lun, Ranran Xie, Yixin Duan, Leina Ma, Wensheng Qiu, Jing Fang, Zhimin Lu

“…Importantly, nuclear GCK interacts with the transcriptional coactivator with PDZ-binding motif (TAZ) and functions as a protein kinase that phosphorylates TAZ T346. …”
Get full text

Enhancing the reverse transcriptase function in Taq polymerase via AI-driven multiparametric rational design by Yulia E. Tomilova, Nikolay E. Russkikh, Igor M. Yi, Elizaveta V. Shaburova, Viktor N. Tomilov, Galina B. Pyrinova, Svetlana O. Brezhneva, Olga S. Tikhonyuk, Nadezhda S. Gololobova, Dmitriy V. Popichenko, Maxim O. Arkhipov, Leonid O. Bryzgalov, Evgeniy V. Brenner, Anastasia A. Artyukh, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Denis V. Antonets, Mikhail K. Ivanov, Mikhail K. Ivanov

“…Several enzymes validated via this procedure were effective in single-enzyme real-time reverse-transcription PCR setups, implying their utility for the development of new tools for real-time reverse-transcription PCR technologies, such as pathogen RNA detection and gene expression analysis. This study illustrates how AI can be effectively integrated with experimental bioengineering to enhance enzyme functionality systematically. …”
Get full text

SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions by Qiwei Jiang, Nana Zheng, Lang Bu, Xiaomei Zhang, Xiaoling Zhang, Yuanzhong Wu, Yaqing Su, Lei Wang, Xiaomin Zhang, Shancheng Ren, Xiangpeng Dai, Depei Wu, Wei Xie, Wenyi Wei, Yasheng Zhu, Jianping Guo

“…Conclusions Our results not only reveal a physiological regulation of PDK1 by E3 ligase SPOP, but also highlight the oncogenic roles of loss-of-function mutations of SPOP or gain-of-function mutations of PDK1 in tumorigenesis through activating the AKT kinase.…”
Get full text

Plasma Extracellular Vesicles Biomarkers Linked to Lower Muscle Mass, Function and Physical Performance in Sarcopenia by Ji Yeon Kim, Tae‐Hwan Gil, Hyo Gyeong Lee, Ji‐Won Shin, Dong‐Hyun Jang, Hyeon Soo Kim, Seung Shin Park, Sang Wan Kim, Chan Soo Shin, Sung Hye Kong, Ok Hee Jeon

“…Hand grip strength was measured using a hydraulic hand dynamometer for muscle function and physical performance based on the Short Physical Performance Battery (SPPB), walking speed and the five‐time‐sit‐to‐stand test. …”
Get full text

Unveiling the Impact of COVID-19 on Ovarian Function and Premature Ovarian Insufficiency: A Systematic Review by Charalampos Voros, Despoina Mavrogianni, Aspasia Minaoglou, Anthi-Maria Papahliou, Vasileios Topalis, Antonia Varthaliti, Dimitris Mathiopoulos, Panagiota Kondili, Menelaos Darlas, Agni Pantou, Sophia Sina, Antonia Athanasiou, Diamantis Athanasiou, Dimitrios Loutradis, Georgios Daskalakis

“…It is characterized by decreased ovarian function, elevated gonadotropin levels, and decreased estradiol. …”
Get full text

Comparison of the effects of metformin and empagliflozin on cardiac function in heart failure with preserved ejection fraction mice by Xiehong Liu, Xiehong Liu, Xiehong Liu, Xiehong Liu, Huiqi Zhao, Huiqi Zhao, Sisi Liu, Sisi Liu, Siao Wen, Siao Wen, Wenjuan Fan, Wenjuan Fan, Qiong Xie, Qiong Xie, Bo Cui, Bo Cui, Lin Zhou, Jianqiang Peng, Jianqiang Peng, Hongwei Pan, Hongwei Pan, Zhaofen Zheng, Zhaofen Zheng, Qinghai Zhang, Qinghai Zhang

“…EMPA downregulated HSP90 mRNA and protein expression; both drugs downregulated TGFβ.ConclusionMET and EMPA improve cardiac fibrosis, diastolic function, and pulmonary congestion in HFpEF mice. …”
Get full text

Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation by Yujuan Qin, Yujuan Qin, Xi Wang, Xi Wang, Xiamin Zhang, Xiamin Zhang, Lianting Nong, Lianting Nong, Qiyan Hou, Yuhong Chen, Yuhong Chen, Yuting Li, Yuting Li, Wenxian Lin, Xiuli Mao, Kezhao Wu, Wenqian Nong, Tonghua Wang, Lingzhang Meng, Lingzhang Meng, Jian Song, Jian Song, Jian Song

“…This study aims to elucidate the role of RA in modulating macrophage function, distribution, and immune responses during bacterial infections.MethodsA murine model of peritonitis was established using Escherichia coli expressing a tdTomato fluorescence marker. …”
Get full text

Iron modulates barrier integrity and stem cell function of small intestine during experimental colitis by Shubin Wang, Xiangjie Yang, Xiangjun Liu, Qin Wen, Lu Xu, Mei Feng, Mei Feng, Jinyi Lang, Dengqun Liu, Dengqun Liu

“…The influence of iron on the barrier integrity and stem cell function was further determined by histology, IEC-6 cell, and enteroid culture. …”
Get full text

A systematic review and functional in-silico analysis of genes and variants associated with amyotrophic lateral sclerosis by Carlos A. Arreola-Aldape, Carlos A. Arreola-Aldape, Carlos A. Arreola-Aldape, Jose A. Moran-Guerrero, Jose A. Moran-Guerrero, Guillermo K. Pons-Monnier, Guillermo K. Pons-Monnier, Rogelio E. Flores-Salcido, Rogelio E. Flores-Salcido, Emmanuel Martinez-Ledesma, Emmanuel Martinez-Ledesma, Luis M. Ruiz-Manriquez, Luis M. Ruiz-Manriquez, K. Rebeca Razo-Alvarez, K. Rebeca Razo-Alvarez, Daniela Mares-Custodio, Daniela Mares-Custodio, Pablo J. Avalos-Montes, Pablo J. Avalos-Montes, Jose A. Figueroa-Sanchez, Jose A. Figueroa-Sanchez, Rocio Ortiz-Lopez, Rocio Ortiz-Lopez, Rocio Ortiz-Lopez, Hector R. Martínez, Hector R. Martínez, Raquel Cuevas-Diaz Duran, Raquel Cuevas-Diaz Duran

Get full text

When polymers meet metals: The changing shape of magnetostrictive polymer composite by A. Pegoretti

Get full text

Electrospun organically modified sepiolite/PVDF coating on polypropylene separator to improve electrochemical performance of lithium-ion battery by Xianli Sun, Jiahao Xu, Xiaoke Zhi, Jingpeng Zhang, Kangwei Hou, Yuhan Bian, Xiaolin Li, Li Wang, Guangchuan Liang

Get full text

Exploring the functional variations of key candidate genes affecting egg production by hypothalamic-pituitary-ovarian axis in chickens by Dandan Wang, Yiqian Zhu, Jiajia Dong, Yihao Zhi, Guanghui Wei, Xiangtao Kang, Xiaojun Liu

“…Association analysis confirmed the significant association of chr3:79510218A>T with egg number from 21 to 43 week of age in Gushi chicken (P = 0.0022) and Guangxi Yao chicken (P = 0.0388), as well as with ovarian PHIP expression levels (P = 0.0010). Functional analysis indicated that the T allele of chr3:79510218A>T enhanced the transcriptional activity and upregulated PHIP expression in vitro by binding transcription factor forkhead box I1 (FOXI1). …”
Get full text

From indicating similarity to establishing online alignment: interactional functions of ye “also” in Mandarin Chinese by Lu Xue, Shuangyun Yao

“…Although the lexical meaning and syntactic properties of ye have been widely discussed, there are few analyses of ye expressions in interactional discourse, let alone analyses examining its interactional functions. …”
Get full text