Harnessing flavonoids to control probiotic function: in situ application of a naringenin-responsive genetic circuit by Brenno Wendler Miranda, Lucas Henrique Junges, Emanuel Maltempi de Souza, Paula Santana Lunardi, Marcelo Müller-Santos

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Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress by Jia-Jun Ren, Xiu-Wen Yuan, Yu-Hao Zhang, Zi-Long Meng, Xing-Wei Liang, Nam-Hyung Kim, Yong-Nan Xu, Ying-Hua Li

“…Furthermore, DIOS treatment upregulated the expression of relevant genes compared to the aged group. …”
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The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem by Fatimah Aljedaani, Yinghui Luo, Yanming Deng, Wouter Smet, Zeeshan Nasim, Xinjing Xu, Umar F. Shahul Hameed, Ting Ting Xiao, Jose Kenyi Gonzalez-Kise, Stefan Arold, Ikram Blilou

“…However, the molecular mechanisms through which it functions in the root meristem remain largely unclear. …”
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Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits by Yan Liu, Chaoyi Song, Qingwen Cui, Hongluan Sun, Chanjuan Jiang, Ruofei Guo, Ruoting He, Zhen Li, Ji Luan, Hailong Wang

“…The truncated mRNA translation rescue strategy will facilitate engineering of multi-domain proteins to enhance their functions.…”
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Evaluation of the functional activity of CD39 ectonucleotidase in regulatory T cells in children with inflammatory bowel diseases by T. V. Radygina, E. G. Sorokina, S. V. Petrichuk, D. G. Kuptsova, O. V. Kurbatova, A. S. Potapov, S. A. Afanasyeva

“…Ectonucleotidase CD39 catalyzes the dephosphorylation of ATP to AMP, followed by conversion to adenosine by CD73. CD39 is expressed in various cell types, including Treg. Aim – evaluate the functional activity of CD39+ in Treg in children with IBD using the luciferin-luciferase method.68 children with IBD were examined. …”
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Functional characterization of COMT genes in Chinese white pear (Pyrus bretschneideri) and their role in lignin synthesis by Xiaofeng Feng, Xiaofeng Feng, Xianang Wu, Xianang Wu, Jiayi Hong, Jiayi Hong, Zihui Guo, Zihui Guo, Yasmin Khan, Yasmin Khan, Dongyi Wei, Dongyi Wei, Honghong Fan, Honghong Fan, Yongping Cai, Yongping Cai

“…Although COMT has been extensively studied in model organisms, its function in pears remains less explored.ResultsIn this study, we identified 29 COMT genes in the pear variety ‘Dangshan Su’, classified into five subfamilies. …”
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IAPP blocks anti-breast cancer function of CD8+T cells via targeting cuproptosis by Dandan Guo, Dandan Guo, Zhijian Huang, Qianqian Wang, Qianqian Wang, Wei Chen, Wei Chen, Yu Huang, Yu Huang, Xinhao Sun, Jian Chen, Shuying Feng, Shuying Feng

“…Thereby downregulation of IAPP reduced cuprotosis of CD8+T or Her2-CAR-T cells to promote the anti-BRCA function both in vitro and in vivo.ConclusionOur research had clarified the function and mechanism of IAPP in CD8+T cells, providing new ideas for improving the diagnosis and treatment of BRCA.…”
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Real-time functional proteomics enhances therapeutic targeting in precision oncology molecular tumor boards by Allison L. Hunt, Jamie Randall, Mahesh M. Mansukhani, Kara Nyberg, Aratara Nutcharoen, Justin Davis, Brian Corgiat, Claudius Mueller, Savannah Melvin, Meenakshi Sharma, Laura Johnston, Whitney Swain, Tamara Abulez, Nicholas W. Bateman, G. Larry Maxwell, John Deeken, Amin Benyounes, Emanuel F. Petricoin, Timothy L. Cannon, Thomas P. Conrads

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Metabolomic Profiling and Functional Characterization of Biochar from Vine Pruning Residues for Applications in Animal Feed by Serena Reggi, Sara Frazzini, Maria Claudia Torresani, Marianna Guagliano, Cinzia Cristiani, Salvatore Roberto Pilu, Martina Ghidoli, Luciana Rossi

“…Biochar has gained interest as a feed ingredient in livestock nutrition due to its functional properties, circularity, potential to reduce environmental impact, and alignment with sustainable agro-zootechnical practices. …”
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Effect of SkQ1 Antioxidant on Structural and Functional Conditions of The Brain in PostResuscitation Period by M. L. Lovat, M. Sh. Avrushchenko, O. A. Averina, V. V. Pavshintsev, I. V. Ostrova, Y. V. Zarzhetsky, V. V. Moroz, M. V. Egorov

“…The aim was to assess the efficacy of mitochondriatargeted antioxidant SkQ1 in prevention of structural and functional abnormalities of brain postresuscitation after cardiac arrest.Materials and methods. …”
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Long-term tuina can inhibit the occurrence of gastroparesis by protecting gastrointestinal function in diabetic rats by Jian-zhen Jiang, Wan-qiu Li, Kun-lin Kuang, Yu-qing Jiang, Zhao-xuan He, Li-juan Zhang, Jing-ya Cao, Dan Wang, Xin-yue Zhang, Zi-lei Tian, Jun Zhu, De-zhong Peng

“…Activities of Ca2+-Mg2+-ATPase and CS enzymes in ileum tissue were also elevated, indicating enhanced GI function. Further analysis showed increased mRNA expressions of Piezo2 and 5-HT4R, and protein expressions of Piezo2, 5-HT, and 5-HT4R in the ileum tissues of the DM + TT group. …”
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Genomic and functional characterization of a Butyricicoccus porcorum strain isolated from human gut microbiota by Ni Zhao, Peiling Geng, Alejandro Gaher Perez, Anakha Chandramana Maya, Brijesh Yadav, Yuxuan Du, Yong Ge

“…ABSTRACT The gut is the most complex microbial ecosystem in the body that greatly influences human immune and metabolic health. However, the functional understanding of gut microbiome is hampered by our limited ability to obtain bacterial cultures for experimental validation, particularly low-abundant species that may carry specific functions but are often overlooked by population-based analyses. …”
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Identification of a distal enhancer of Ucp1 essential for thermogenesis and mitochondrial function in brown fat by Duo Su, Tingting Jiang, Yulong Song, Die Li, Siyuan Zhan, Tao Zhong, Jiazhong Guo, Li Li, Hongping Zhang, Linjie Wang

“…In vivo, lentivirus-mediated repression of Ucp1-En4 was injected into iBAT, resulting in impacted iBAT thermogenic capacity and impaired iBAT mitochondrial function under cold acclimation conditions. Studying the functional enhancers regulating Ucp1 expression in iBAT will provide important insights into the regulatory mechanisms of BAT activity.…”
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Osteogenic and Antibacterial Response of Levofloxacin-Loaded Mesoporous Nanoparticles Functionalized with <i>N</i>-Acetylcysteine by Alberto Polo-Montalvo, Natividad Gómez-Cerezo, Mónica Cicuéndez, Blanca González, Isabel Izquierdo-Barba, Daniel Arcos

“…Herein we propose a nanosystem composed of mesoporous bioactive glass nanoparticles (MBGN) loaded with levofloxacin and functionalized with N-acetylcysteine (NAC), aiming to offer an alternative to current treatments. …”
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LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease by Mi Bai, Mengqiu Wu, Mingzhu Jiang, Jia He, Xu Deng, Shuang Xu, Jiaojiao Fan, Mengqiu Miao, Ting Wang, Yuting Li, Xiaowen Yu, Lin Wang, Yue Zhang, Songming Huang, Li Yang, Zhanjun Jia, Aihua Zhang

“…Mechanistically, LONP1 downregulation caused mitochondrial accumulation of the LONP1 substrate, 3‐hydroxy‐3‐methylglutaryl‐CoA synthase 2 (HMGCS2), which disrupted mitochondrial function and further accelerated CKD progression. Finally, computer‐aided virtual screening was performed, which identified a novel LONP1 activator. …”
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Non-immune targeting of CXCR3 compromises mitochondrial function and suppresses tumor growth in glioblastoma by Travis Yui Hei Chan, Bo Chen, Wanjun Tang, Henry Hei Chan, Yogesh K. H. Wong, Ethan C. L. Wong, Junbo Liao, Anson Cho-Kiu Ng, Jenny Sum Yee Wong, Gilberto Ka-Kit Leung, Karrie M. Kiang

“…However, emerging evidence suggests that its functions may extend beyond the immune system, particularly in cancer, where its roles remain to be elucidated. …”
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Physicochemical and techno-functional characterization of soluble proteins extracted by ultrasound from the cricket Acheta domesticus by Salvador O. Cruz-López, Héctor B. Escalona-Buendía, Isadora Martinez-Arellano, Julieta Domínguez-Soberanes, Yenizey M. Alvarez-Cisneros

“…The objective of this work was to evaluate the physicochemical and techno-functional properties of the different soluble protein fractions of the cricket Acheta domesticus using various methods: grinding (CF), defatting (DCF), alkalinization (SPA), and ultrasound-assisted extraction (SPS). …”
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Functional characterization of seven terpene synthases from Ophiopogon japonicus via engineered Escherichia coli by Wen Xu, Qing Ma, Tong Chen, Haiyan Zhang, Guanglei Ma, Sibongile Mafu, Juan Guo, Xiaohui Fan, Guanghong Cui, Baolong Jin

“…The candidate terpene synthase genes were identified from the root and leaf transcriptome data of O. japonicus in Zhejiang and the functions of these enzymes were identified using engineered Escherichia coli. …”
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Functional characterization of antennae-enriched chemosensory protein 4 in emerald ash borer, Agrilus planipennis by Ren Li, Zehua Wang, Fan Yang, Guanghang Qiao, Jingjing Tu, Ang Sun, Shanning Wang

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Major nuclear locales define nuclear genome organization and function beyond A and B compartments by Omid Gholamalamdari, Tom van Schaik, Yuchuan Wang, Pradeep Kumar, Liguo Zhang, Yang Zhang, Gabriela A Hernandez Gonzalez, Athanasios E Vouzas, Peiyao A Zhao, David M Gilbert, Jian Ma, Bas van Steensel, Andrew S Belmont

“…Together, our results underscore the importance of considering genome organization relative to nuclear locales for a more complete understanding of the spatial and functional organization of the human genome.…”
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