allograft inflammatory factor 1 functions as a pro-inflammatory cytokine in the oyster, Crassostrea ariakensis. by Ting Xu, Jiasong Xie, Baojian Zhu, Xiao Liu, Xinzhong Wu

“…Therefore we investigated the functions of C. ariakensis AIF-1 (Ca-AIF1) and its antibody (anti-CaAIF1) in oyster RLO/LPS-induced disease and inflammation. …”
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Selective amino acid formulation enhances anion secretion and restores function in cystic fibrosis mutations by Astrid Grosche, Anusree Sasidharan, Matthias Salathe, Nathalie Baumlin, Damiano Angoli, Sreekala Prabhakaran, Xiaodong Xu, Sadasivan Vidyasagar

“…IntroductionIn cystic fibrosis (CF), most CFTR mutations cause partial (Class II) or complete (Class I) loss of function. Modulators (VX) can improve CFTR function in Class II mutations but are ineffective for Class I mutations and may cause side effects, resulting in tolerability issues with concerns about long-term safety. …”
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Effect of Aerobic Treadmill Exercise Regulating Glucose Transporter 9 on Renal Function in Hyperuricemia Rats by LIANG Jiarong, YANG Huan, CUN Yongdan, WANG Lihuan, LI Yijie, YE Bin

“…Hematoxylin-eosin (HE) was used to stain the renal histopathological section, and the microscope (×400) was used to observe the structural changes of renal tubules and glomerulus; the peroxidase method was used to detect the renal function indexes such as uric acid (UA), creatinine (Cre) and urea nitrogen (BUN); enzyme-linked immunosorbent assay (ELISA) method was used to determine the contents of interleukin-1β (IL-1β) in serum and kidney tissues; Western blot was used to detect the expressions of Caspase-1, IL-1β and glucose transporter 9 (GLUT-9) in the kidney tissues.Results① Pathological examination results: there was not obvious pathological changes were observed in the kidney tissues of the Control group; in the Model group, the renal tissue capsule was incomplete, the glomerulus was deformed and atrophic, and the renal tubules were disordered; in the Exercise group, the number of inflammatory cell infiltration was significantly decreased, the renal tubular structure was relatively intact, and the pathological changes such as cytoplasmic vacuolation of renal tubular epithelial cells were significantly decreased. ② UA, Cre and BUN content of serum: compared with the Control group, UA, BUN and Cre content in serum of the Model group and Exercise group was significantly increased (<italic>P</italic>&lt;0.05); compared with the Model group, UA, BUN and Cre content in serum of the Exercise group were significantly decreased (<italic>P</italic>&lt;0.05). ③ Serum and kidney IL-1β content: compared with the Control group, the expression of IL-1β in serum and kidney of the Model group and the Exercise group were significantly increased (<italic>P</italic>&lt;0.05); compared with the Model group, the expression of IL-1β in serum and kidney of the Exercise group was significantly decreased (<italic>P</italic>&lt;0.05). ④ Expressions of Caspase-1, IL-1β and GLUT-9 protein: compared with the Control group, the expressions of Caspase-1, IL-1β and GLUT-9 protein in kidney tissues of the Model group and the Exercise group were significantly increased (<italic>P</italic>&lt;0.05); compared with the Model group, the expressions of Caspase-1, IL-1β, and GLUT-9 protein in kidney tissues of the Exercise group were significantly decreased (<italic>P</italic>&lt;0.05).ConclusionAerobic treadmill exercise can effectively reduce the level of uric acid and inflammatory response of hyperuricemia rats, and improve renal function, which may be related to the regulation of GLUT-9 protein expression.…”
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Identification of key genes and pathways in the following pancreatic ductal adenocarcinoma (PDAC) using integrated bioinformatics analysis by Manish Shukla, Jai Chand Patel, Meenakshi Shukla

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Exploring hypoxia-related genes in spinal cord injury: a pathway to new therapeutic targets by Shihuan Cheng, Le Li, Mengmeng Xu, Ningyi Ma, Yinhua Zheng

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Joint analysis of whole-genome methylation and transcriptome in avian pullorum disease and validation of gene function by Jiongwen Wu, Weiming Liang, Aijun Liu, Xiaomeng Wang, Zhexia Fan, Xuerong Ma, Shuya Chen, Cheng Fang, Xiquan Zhang, Qingbin Luo

“…The function of the key gene MSX2 was verified by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay. …”
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Condensed tannin from Caragana korshinskii extraction and protection effects on intestinal barrier function in mice by Xiaoyu Niu, Wei Qu, Zhiyu Chen, Hui Li, Peinan Liu, Mei Sun, Jing Yang, Yuanyuan Xing, Dabiao Li

“…Caragana korshinskii tannins (CKT) were extracted by response surface methodology and the protection effect of CKT on the jejunal mucosal barrier function of mice was investigated. Firstly, this work presents the extraction, purification and characterization of CKT. …”
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SPOCK2 controls the proliferation and function of immature pancreatic β-cells through MMP2 by Katarzyna Blaszczyk, Anna P. Jedrzejak, Natalia Ziojla, Ekaterina Shcheglova, Karolina Szarafin, Artur Jankowski, Christine A. Beamish, Jolanta Chmielowiec, Omaima M. Sabek, Ashok Balasubramanyam, Sanjeet Patel, Malgorzata Borowiak

“…In contrast, newly formed β-cells, including SC-β-cells, display higher proliferative capacity and distinct transcriptional and functional profiles. Through bidirectional expression modulation and single-cell RNA-seq, we identified SPOCK2, an ECM protein, as an inhibitor of immature β-cell proliferation. …”
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Effects of Shikonin on the Functions of Myeloid Dendritic Cells in a Mouse Model of Severe Aplastic Anemia by Mengying Zheng, Bingnan Liu, Yuanyuan Shao, Luogang Hua, Rong Fu, Huaquan Wang, Ting Wang, Weiwei Qi, Zonghong Shao, Chunyan Liu

“…This study is aimed at investigating the effects of shikonin, a pyruvate kinase M2 (PKM2) inhibitor, on the functions of myeloid dendritic cells (mDCs) in a mouse model of severe aplastic anemia (AA) generated by total body irradiation and lymphocyte infusion. …”
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Functional link between mitochondria and Rnr3, the minor catalytic subunit of yeast ribonucleotide reductase by Isaac Corcoles-Saez, Jean-Luc Ferat, Michael Costanzo, Charles M. Boone, Rita S. Cha

“…While Rnr1 is required for DNA replication and DNA damage repair, the function(s) of Rnr3 is unknown. Here, we show that carbon source, an essential nutrient, impacts Rnr1 and Rnr3 abundance: Non-fermentable carbon sources or limiting concentrations of glucose down regulate Rnr1 and induce Rnr3 expression. …”
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In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains by Chun-Yan Yeung, Jen-Shiu Chiang Chiau, Wai-Tao Chan, Chun-Bin Jiang, Mei-Lien Cheng, Hsuan-Liang Liu, Hung-Chang Lee

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Analysis of 17,576 potentially functional SNPs in three case-control studies of myocardial infarction. by Dov Shiffman, John P Kane, Judy Z Louie, Andre R Arellano, David A Ross, Joseph J Catanese, Mary J Malloy, Stephen G Ellis, James J Devlin

“…In the current study we tested 17,576 SNPs that could affect gene function or expression. In order to use genotyping resources efficiently, we staged the testing of these SNPs in three case-control studies of MI. …”
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Impaired cognitive function and decreased monoamine neurotransmitters in the DNAJC12 gene knockout mouse model by Shunan Wang, Ming Shen, Bo Pang, Bo Zhou, Yuan Yuan, Mei Lu, Xiangling Deng, Min Yang, Shufang Liu, Qiong Wang, Mei Xue, Qisheng Xia, Zhixin Zhang

“…The knockout mice exhibit hyperphenylalaninemia, impaired cognitive function, and decreased monoamine neurotransmitters. …”
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Form follows function: Morphological and immunohistological insights into epithelial–mesenchymal transition characteristics of tumor buds by Kathrin Enderle-Ammour, Moritz Bader, Theresa Dorothee Ahrens, Kai Franke, Sylvia Timme, Agnes Csanadi, Jens Hoeppner, Birte Kulemann, Jochen Maurer, Philip Reiss, Oliver Schilling, Tobias Keck, Thomas Brabletz, Elmar Stickeler, Martin Werner, Ulrich Friedrich Wellner, Peter Bronsert

“…In cancer biology, the architectural concept “form follows function” is reflected by cell morphology, migration, and epithelial–mesenchymal transition protein pattern. …”
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CIRBP Enhances the Function of Yak Cumulus Cells by Activating AMPK/mTOR-Mediated Mitophagy by Rui Zhang, Yan Cui, Yangyang Pan, Meng Wang, Sijiu Yu, Ruihua Xu, Wenbin Ma, Junqian Wang, Donglan Zhong, Zhengxing Jiao

“…In this study, we found that CIRBP induced mitophagy through the AMPK/mTOR pathway to improve the function of yak cumulus cells (YCCs). We observed that low temperatures (32 °C) activated autophagy, increased E2 and P4 secretion, and up-regulated CIRBP expression. …”
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Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry by Parichart Tesena, Ruethaiwan Vinijkumthorn, Amornthep Kingkaw, Petchpailin Yanyongsirikarn, Khajornpol Phasuk, Sekkarin Ploypetch, Narumon Phaonakrop, Sittiruk Roytrakul, Wanwipa Vongsangnak, Nawarus Prapaiwan

“…Immunohistochemical staining (IHC) towards immunolocalizing Wnt signature protein, particularly the Wnt2B functional signal, further verified its higher expression in early EMN compared to other groups. …”
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Identification of key interactive genes and their potential biological functions in type 2 diabetes and Sjögren’s syndrome by Yibing Wang, Huilin Zeng, Xiangjie Hu, Guangzhao Zhang, Liangliang Zhou, Xingyuan Yu, Yuchun Zhang

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Biological functions of extracellular vesicle double C2-like domain beta in cervical cancer by Sangavi Eswaran, Samatha Bhat, Dinesh Upadhya, Roshan Mascarenhas, Shama Prasada Kabekkodu

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The biological function and prognostic significance of ferroptosis-related genes in clear cell renal cell carcinoma by Huizhen Ye, Xiwen Wu, Gehao Liang

“…Furthermore, high-risk individuals demonstrated elevated expression of immune checkpoint genes and enhanced antitumor immunity, suggesting that these patients may benefit from immunotherapy.ConclusionFerroptosis-related genes play a critical role in the biological functions of ccRCC cells. …”
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Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content by Fabio Papes, Antonio P. Camargo, Janaina S. de Souza, Vinicius M. A. Carvalho, Ryan A. Szeto, Erin LaMontagne, José R. Teixeira, Simoni H. Avansini, Sandra M. Sánchez-Sánchez, Thiago S. Nakahara, Carolina N. Santo, Wei Wu, Hang Yao, Barbara M. P. Araújo, Paulo E. N. F. Velho, Gabriel G. Haddad, Alysson R. Muotri

“…We identify a mechanism through which TCF4 loss-of-function leads to decreased Wnt signaling and then to diminished expression of SOX genes, culminating in reduced progenitor proliferation in vitro. …”
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