Reclassification of Two MLH1 Variants of Uncertain Significance Utilizing Clinical and Functional Data by Jane Hübertz Frederiksen, Ulf Birkedal, Sarah Bachmann, Elisabeth Victoria Eliesen, Lene Juel Rasmussen, Katja Venborg Pedersen, Lana Al‐Zehhawi, Susanne E. Boonen, Lotte Krogh, Karina Rønlund, Lise Graversen, Jannie Assenholt, Kjeld Schmiegelow, Karin Wadt, Anne‐Marie Gerdes, Thomas v. O. Hansen

“…Conclusion By applying all collected data and functional results we propose to reclassify the c.696_698del, p.…”
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Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired by Yiqiao Chen, Chiara Tontini, Isabella Tosi, Jonathan N. Barker, Paola Di Meglio, Christopher E. M. Griffiths, Christopher E. M. Griffiths, Rajia Bahri, Silvia Bulfone-Paus

“…Thus, this indicates that psoriasis impacts the functionality of circulating CD8 T cells.…”
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Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein by Maliki Ankavay, Nathalie Da Silva, Angela Pollán, Noémie Oechslin, Katja Dinkelborg, Patrick Behrendt, Darius Moradpour, Jérôme Gouttenoire

“…Methods: We exploited transposon-mediated random insertion and selection of viable clones to identify sites in the HEV open reading frame 2 (ORF2) protein, corresponding to the viral capsid, allowing for the insertion of reporter sequences in a functional context. Results: Short sequence insertions (5 amino acids) were tolerated at four distinct sites in the C-terminal region of the ORF2 protein, without significantly affecting viral capsid expression and subcellular localization as well as virus production. …”
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Identification and functional characterisation of DNA methylation differences between East- and West-originating Finns by Joanna Ciantar, Saara Marttila, Sonja Rajić, Daria Kostiniuk, Pashupati P Mishra, Leo-Pekka Lyytikäinen, Nina Mononen, Marcus E Kleber, Winfried März, Mika Kähönen, Olli Raitakari, Terho Lehtimäki, Emma Raitoharju

“…Our results indicate that DNA methylation differences between East and West Finns may have a functional role in mediating the cardiometabolic disease discrepancy between the sub-populations.…”
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Linalool exhibit antimicrobial ability against Elizabethkingia miricola by disrupting cellular and metabolic functions by Mingwang He, Weiming Zhong, Rongsi Dai, Su Long, Ying Zhou, Tongping Zhang, Boyang Zhou, Tao Tang, Linlin Yang, Sifan Jiang, Wenbin Xiao, YanJiao Fu, Jiajing Guo, Zhipeng Gao

“…Linalool disrupted cell division and RNA function in E. miricola, and the cells responded to linalool-induced oxidative damage by up-regulating the expression of msrB and katG genes. …”
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METTL9 sustains vertebrate neural development primarily via non-catalytic functions by Azzurra Codino, Luca Spagnoletti, Claudia Olobardi, Alessandro Cuomo, Helena Santos-Rosa, Martina Palomba, Natasha Margaroli, Stefania Girotto, Rita Scarpelli, Shi-Lu Luan, Eleonora Crocco, Paolo Bianchini, Andrew J. Bannister, Stefano Gustincich, Tony Kouzarides, Riccardo Rizzo, Isaia Barbieri, Federico Cremisi, Robert Vignali, Luca Pandolfini

“…Overall, we demonstrate a developmental function of Mettl9 and link it to a 1MH-independent pathway, namely, the maintenance of the secretory system, which is essential throughout neural development.…”
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Common Neural Correlates for Subjective and Objective Sleepiness Indices: A Functional Connectivity Study by Motomura Y, Kitamura S, Oba K, Katsunuma R, Terasawa Y, Hida A, Moriguchi Y, Mishima K

“…The results of this study provide valuable insights into the effects of sleep deprivation and total sleep deprivation experienced in daily life on the brain and offer a new perspective on the expression of sleepiness in the brain.Keywords: functional connectivity, sleepiness, psychomotor vigilance task, default mode network, middle temporal cortex, thalamus…”
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Structural and functional characteristics of the T helpers 17 subpopulation in allergic diseases of the respiratory organs in children by E. V. Prosekova, N. G. Plekhova, A. I. Turyanskaya, V. A. Sabynych

“…A diagnostically significant increase in the absolute and relative amounts of T helper 17 type with detectable changes in their functional characteristics, i.e., by the CCR6 chemokine receptor (CD196) expression levels, and presence of IL17A interleukin in children with atopic asthma and allergic rhinitis. …”
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Mettl3/Eed/Ythdc1 regulatory axis controls endometrial receptivity and function by Xiujuan Hu, Jincheng Li, Chenyue Ding, Jing Jiang, Wenjuan Xia, Jiafeng Lu, Huapeng Su, Ming Zhang, Hong Li, Ji-long Liu, Yuan Lin, Qingxia Meng, Boxian Huang

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Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1 by Xinxin Li, Jinghua Cui, Zanbo Ding, Ziyan Tian, Yiming Kong, Linghai Li, Yang Liu, Wen Zhao, Xueying Chen, Han Guo, Zhengshuo Cui, Xinwei Li, Jing Yuan, Huina Zhang

“…Bacterial extracellular vesicles (BEVs) released by Gram-negative bacteria modulate host cell functions by delivering bacterial components to host cells. …”
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Chaperone-mediated autophagy in fish: A key function amid a changing environment by Simon Schnebert, Emilio J Vélez, Maxime Goguet, Karine Dias, Vincent Véron, Isabel García-Pérez, Lisa M Radler, Emilie Cardona, Stéphanie Fontagné-Dicharry, Pierre Van Delft, Franziska Dittrich-Domergue, Amélie Bernard, Florian Beaumatin, Amaury Herpin, Beth Cleveland, Iban Seiliez

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Lipid metabolism of apoptotic vesicles accelerates cutaneous wound healing by modulating macrophage function by Yuan Qin, Xin Chen, Lili Bao, Lili Ren, Geng Dou, Jianing Lian, Shujuan Xing, Zihan Li, Feng Ding, Wen Qin, Xulin Liu, Bin Zhu, Shiyu Liu, Zuolin Jin, Xiaoshan Yang

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Natural killer cells in the human lung tumor microenvironment display immune inhibitory functions by Diane Damotte, Laurence Zitvogel, Marco Alifano, Marie-Caroline Dieu-Nosjean, Gabriela Bindea, Jeremy Goc, Jules Russick, Pierre-Emmanuel Joubert, Mélanie Gillard-Bocquet, Carine Torset, Maxime Meylan, Florent Petitprez, Marie-Agnes Dragon-Durey, Solenne Marmier, Aditi Varthaman, Nathalie Josseaume, Claire Germain, Pierre Validire, Ludovic Fournel, Audrey Lupo, Isabelle Cremer

“…Emerging data reveal NK cell dysfunction within the tumor microenvironment (TME) through checkpoint inhibitory molecules associated with a regulatory phenotype.Objective We aimed at analyzing the gene expression profile of intratumoral NK cells compared with non-tumorous NK cells, and to characterize their inhibitory function in the TME.Methods NK cells were sorted from human lung tumor tissue and compared with non- tumoral distant lungs.Results In the current study, we identify a unique gene signature of NK cell dysfunction in human non-small cell lung carcinoma (NSCLC). …”
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Nuclear receptor corepressor 1 controls regulatory T cell subset differentiation and effector function by Valentina Stolz, Rafael de Freitas e Silva, Ramona Rica, Ci Zhu, Teresa Preglej, Patricia Hamminger, Daniela Hainberger, Marlis Alteneder, Lena Müller, Monika Waldherr, Darina Waltenberger, Anastasiya Hladik, Benedikt Agerer, Michael Schuster, Tobias Frey, Thomas Krausgruber, Sylvia Knapp, Clarissa Campbell, Klaus Schmetterer, Michael Trauner, Andreas Bergthaler, Christoph Bock, Nicole Boucheron, Wilfried Ellmeier

“…We also reveal a critical role for this epigenetic regulator in supporting the suppressive functions of Treg cells in vivo.…”
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Discovery of functional PRRs for the fungal elicitor Xyn11/eix in Prunus fruit trees by Andree Alvarez, Uri Aceituno-Valenzuela, Meirav Leibman-Markus, Daniela Muñoz, Carlos Rubilar, Franco Figueroa, Manuel Pinto, Mauricio Latorre, Claudia Stange, Adi Avni, Maya Bar, Lorena Pizarro

“…Additionally, the research identified functional orthologues of LeEIX2, the receptor for Xyn11/eix in Solanum lycopersicum, within both Prunes genomes. …”
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An in vivo systemic massively parallel platform for deciphering animal tissue-specific regulatory function by Ashley R. Brown, Ashley R. Brown, Grant A. Fox, Grant A. Fox, Irene M. Kaplow, Irene M. Kaplow, Irene M. Kaplow, Alyssa J. Lawler, Alyssa J. Lawler, BaDoi N. Phan, BaDoi N. Phan, BaDoi N. Phan, Lahari Gadey, Lahari Gadey, Morgan E. Wirthlin, Morgan E. Wirthlin, Easwaran Ramamurthy, Easwaran Ramamurthy, Gemma E. May, Ziheng Chen, Qiao Su, Qiao Su, C. Joel McManus, Robert van de Weerd, Robert van de Weerd, Andreas R. Pfenning, Andreas R. Pfenning, Andreas R. Pfenning

“…While genome-wide association studies are swiftly identifying non-coding human disease-associated loci, decoding regulatory mechanisms is challenging due to causal variant ambiguity and their specific tissue impacts.Methods: Massively parallel reporter assays (MPRAs) are widely used in cell culture to study the non-coding enhancer regions, linking genome sequence differences to tissue-specific regulatory function. However, widespread use in animals encounters significant challenges, including insufficient viral library delivery and library quantification, irregular viral transduction rates, and injection site inflammation disrupting gene expression. …”
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Genetic Polymorphisms Affect Mouse and Human Trace Amine-Associated Receptor 1 Function. by Xiao Shi, Nicole A R Walter, John H Harkness, Kim A Neve, Robert W Williams, Lu Lu, John K Belknap, Amy J Eshleman, Tamara J Phillips, Aaron Janowsky

“…Finally, we demonstrate that SNPs in human TAAR1 alter its function, resulting in expressed, but functional, sub-functional and non-functional receptors. …”
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VERBS MEANING NEGATIVE ATTENDING CIRCUMSTANCES IN THE MODERN RUSSIAN LANGUAGE: SYSTEMATIC AND FUNCTIONAL ASPECTS by Van Cyan

“…The article studies functioning of these verbs in ictional texts, contextual and situational conditions of their realization are analyzed as well. …”
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INFLUENCE OF IMMUNOMODULATING AGENTS IMMUNOVAK VP-4 AND PROFETAL ON FUNCTIONAL ACTIVITY OF MONONUCLEAR LEUKOCYTES by E. A. Lebedinskaya, N. K. Akhmatova, O. V. Lebedinskaya, V. A. Chereshnev, S. U. Rodionov, M. V. Kiselevsky

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The extended functional neuroanatomy of emotional processing biases for masked faces in major depressive disorder. by Teresa A Victor, Maura L Furey, Stephen J Fromm, Patrick S F Bellgowan, Arne Öhman, Wayne C Drevets

“…<h4>Method</h4>Unmedicated-depressed participants with MDD (n=22) and healthy controls (HC; n=25) underwent functional MRI as they viewed face stimuli showing sad, happy or neutral face expressions, presented using a backward masking design. …”
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