Clinical phenotype and functional influence of GRIN2A variants in epilepsy‐aphasia syndrome by Lu Zhang, Yiran Duan, Rui Ma, Jiaqi Han, Na Pan, Lehong Gao, Yuping Wang

“…We assess the variants' harmfulness through a variety of functional experiments, including evaluating the expression level of the mutated protein and the resulting changes in electrophysiological activities. …”
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The incoherent feed‐forward loop can generate non‐monotonic input functions for genes by Shai Kaplan, Anat Bren, Erez Dekel, Uri Alon

“…This motif was shown to act as a pulse generator and response accelerator of gene expression. Here we consider an additional function of this motif: the I1‐FFL can generate a non‐monotonic dependence of gene expression on the input signal. …”
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Identification and functional analysis of novel SPTB and ANK1 mutations in hereditary spherocytosis patients by Charuwan Panarach, Chaiwat Netsawang, Issarang Nuchprayoon, Kamonlak Leecharoenkiat

“…To investigate the functional consequences of these mutations, we studied their pathogenicity using in vitro culture erythroblast derived from CD34 + stem cells. …”
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The application of Gene Function Markers for identification of high quality of Vietnamese traditional rice varieties by Ky Huynh, Manh Nguyen Van, An Tinh Pham, Nguyen Pham, Nhu Dien Huynh, Thanh Le

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HP1 loses its chromatin clustering and phase separation function across evolution by Sanâa Bensaha, Dominika Lewandowska, Fernando Muzzopappa, Stephanie Hutin, Mark D. Tully, Michela Anfossi, Florence M. Cammas, Christophe Normand, Fabian Erdel

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ENL mutation and AML: a new model that reveals oncogenic condensate's function in leukemogenesis by Zhong Fan, Yanan Jiang, Xiaotian Zhang

“…Precise regulation of gene expression is essential for proper development and the maintenance of homeostasis in organisms. …”
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The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster. by Shuwa Xu, Nathaniel Hafer, Blessing Agunwamba, Paul Schedl

“…By contrast, orb2 is broadly expressed in the soma; and previous studies have revealed important functions in asymmetric cell division, viability, motor function, learning, and memory. …”
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Investigation on the Role of Medical Recombinant Human-Derived Collagen Functional Dressings in Wound Healing by Xiaoxiao GAI, Xiaoxia SUN, Wenqian MA, Zhenhua LIN, Xinyuan LI, Chenghu LIU

“…After animal surgery, the surgical wound was taken at 1 week, 4 weeks and 13 weeks, respectively, for hematoxylin eosin (HE) staining and immunohistochemistry to observe the inflammatory response and CD31 expression of the wound. Results Medical recombinant human-derived collagen functional dressing promotes cell proliferation and migration, enhances wound angiogenesis by upregulating the expression of VEGF, FGF, and CD31 in human dermal vascular endothelial cells (HDVEC) and human vascular endothelial cells (HVEC), thereby improving local blood supply to the wound, regulating the inflammatory response of the wound, and accelerating wound healing. …”
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The application of Gene Function Markers for identification of high quality of Vietnamese traditional rice varieties by Ky Huynh, Manh Nguyen Van, An Tinh Pham, Nguyen Pham, Nhu Dien Huynh, Thanh Le

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ACSL4 predicts rapid kidney function decline in patients with diabetic kidney disease by Rui Shen, Xin Yu, Caifeng Shi, Yi Fang, Chunsun Dai, Yang Zhou

“…The associations between the ACSL4 level and clinical characteristics as well as rapid kidney function decline defined as an estimated glomerular filtration rate (eGFR) slope ≤ -5 ml/min/1.73m2/year were analyzed.ResultsACSL4 was mainly expressed in tubular epithelial cells. …”
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The PINK1-Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells. by Aaron V Bradshaw, Philip Campbell, Anthony H V Schapira, Huw R Morris, Jan-Willem Taanman

“…Our findings suggest that the PINK1 -Parkin mitophagy signalling pathway is not functional in PBMCs. Therefore, PBMCs are not a suitable biosample for analysis of mitophagy function in Parkinson disease patients.…”
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The paradox of hnRNPK: both absence and excess impair skeletal muscle function in mice by Yongjie Xu, Yuxi Wang, Xiaofang Cheng, Mengjia Zhang, Nuo Chen, Jiahua Guo, Yueru Huang, Quanxi Li, Tianyu Li, Tiantian Meng, Cencen Li, Pengpeng Zhang, Haixia Xu

“…In adult mice, both the loss and gain of hnRNPK function led to reduced muscle mass, decreased fiber size, and compromised skeletal muscle homeostasis. …”
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Bioinformatics-based prognostic value and in vitro functional validation of PTK6 in cutaneous melanoma by Yanyan Niu, Yanyan Niu, Xiaoyu Liu, Aixiu Shi, Danli Tang, Xiaodong Yao, Yan Lu

“…Bioinformatics analysis evaluated PTK6 expression and its impact on prognosis. GO and KEGG analyses explored biological functions of PTK6-related differentially expressed genes (DEGs). …”
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Single-cell transcriptomics reveals the heterogeneity and function of mast cells in human ccRCC by Xiyu Song, Xiyu Song, Xiyu Song, Jianhua Jiao, Jianhua Jiao, Jiayang Qin, Wei Zhang, Weijun Qin, Weijun Qin, Shuaijun Ma, Shuaijun Ma

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Molecular cloning and functional identification of an apple flagellin receptor MdFLS2 gene by Chen-hui QI, Xian-yan ZHAO, Han JIANG, Hai-tao LIU, Yong-xu WANG, Da-gang HU, Yu-jin HAO

“…Furthermore, the ectopic expression of MdFLS2 complemented the function of the Arabidopsis fls2 mutant and conferred enhanced flg22 tolerance to the transgenic Arabidopsis, suggesting that MdFLS2 acts as a positive regulator in the response to pathogens in apple.…”
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Klf9 Loss of Function Protects Against Glucocorticoids Induced Skeletal Muscle Wasting by Yujie Zhang, Jingran Hao, Yueyao Feng, Tongtong Qiu, Jinjin Wu, Xuenan Zhou, Heng Fan, Yongsheng Chang

“…We performed Klf9 gain‐ or loss‐of‐function studies in differentiated C2C12 myotubes using lentiviruses encoding Klf9 or the shRNA specific to Klf9 in vitro. …”
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HCF-1 as a key modulator of OGT function and O-GlcNAcylation in the liver by Vaibhav Kapuria, Ayushma, Winship Herr, Shilpi Minocha

“…Abstract Host cell factor-1 (HCF-1) is a transcriptional coregulator essential for maintaining liver function and cellular metabolism. O-linked N-acetylglucosamine transferase (OGT) is a key nutrient-sensing enzyme that catalyzes protein O-GlcNAcylation, a critical post-translational modification regulating metabolic pathways. …”
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Aquaporin 5 polymorphisms and rate of lung function decline in chronic obstructive pulmonary disease. by Nadia N Hansel, Venkataramana Sidhaye, Nicholas M Rafaels, Li Gao, Peisong Gao, Renaldo Williams, John E Connett, Terri H Beaty, Rasika A Mathias, Robert A Wise, Landon S King, Kathleen C Barnes

“…<h4>Conclusions</h4>Polymorphisms in AQP5 were associated with rate of lung function decline in continuous smokers with COPD. A missense mutation modulates AQP-5 expression in response to cigarette smoke extract and shear stress. …”
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Investigating the protective effect of hydroxylated fullerenes on cognitive function in rats with temporal lobe epilepsy by Xiaoqing Wang, Shuang Tian, Zhenzhen Qu, Ran Meng, Guangxiao Ni, Min Liu, Huifang Cao

“…Abstract The objective of this study was to explore the protective effects of hydroxy fullerenes (HFs) on cognitive function in rats with temporal lobe epilepsy (TLE) and to elucidate the underlying mechanisms. …”
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Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cells by Yue He, Jian-Qing Xu, Jing-Jing Zhang, Zhen-You Liu, Chen Ji, Yang Liu, Yun-Fan Wang, Ming Wang, Yu-Mei Wu, Yan Wang

“…The downstream mechanism of KCP-mediated resistance to paclitaxel was then examined using human gene chip detection and IPA bioinformatics analysis, and qPCR analysis was used to validate its downstream genes.Results①Functional studies of SiHa cells showed that KCP knockout (sgRNA) inhibited colony formation and proliferation of SiHa cells in the presence of paclitaxel (p&lt;0.05). ②Using a whole human genome microarray, a total of 491 differentially expressed genes were identified in KCP knockout versus the NC SiHa cells. …”
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