Siglec-15 antibody–GM-CSF chimera suppresses tumor progression via reprogramming tumor-associated macrophages by Jun Li, Hongyan Li, Zemeng Ma, Xiaoyao Hao, Wenwen Dai, Jinyu Liu, Mingjiu Chen, Shuang Qu, Quanli Zhang, Jiajing Luo, Shouyong Gu, Dihan Zhu, Ke Zen

“…Background Sialic acid-binding immunoglobulin-like lectin (Siglec)-15-expressing tumor-associated macrophages (TAMs) drive immunosuppression in the tumor microenvironment (TME), promoting CD8+ T cell exhaustion and limiting immunotherapy efficacy. …”
Get full text

PRRG4 Brain-Specific Conditional Knockout Mice Display Autism Spectrum Disorder-Like Behaviors by Luxi Shen, Lan Chen, Yuping Tang, Yeyao Yan, Ting Xiong, Yong Liu, Hongzhi Li, Haihua Gu

“…These findings provide evidence for a comprehensive understanding of PRRG4 function in vivo and support the association between PRRG4 loss and ASD phenotypes observed in WAGR syndrome.…”
Get full text

From spheroids to organoids: next-generation models for CAR-T cell therapy research in solid tumors by Mégane Jassin, Alix Block, Alix Block, Laury Désiront, Louise Vrancken, Louise Vrancken, Céline Grégoire, Frédéric Baron, Frédéric Baron, Grégory Ehx, Grégory Ehx, Thi Tham Nguyen, Jo Caers, Jo Caers

“…Chimeric Antigen Receptor T-cell (CAR-T) therapy is a revolutionary immunotherapy involving the genetic modification of T cells to express chimeric receptors targeting specific tumor antigens. …”
Get full text

Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia by Manuel Varas-Godoy, Stephanie Acuña-Gallardo, Sebastian Venegas-Duarte, Charlotte Hill, Albano Caceres-Verschae, Ornella Realini, Lara J. Monteiro, Gabriela Zavala, Maroun Khoury, Roberto Romero, Gregory Rice, Sebastian E. Illanes

“…Although its precise etiology is still unknown, there is evidence suggesting that it may be a consequence of impaired decidual and stromal cell function. Recently, a stem cell population derived from endometrial tissue and isolated from menstrual effluent called menstrual stem cells (MenSCs) has been identified. …”
Get full text

Mechanistic Exploration of Yiqi Zengmian in Regulating the Microenvironment as an Immunopotentiator with the Beijing Bio-Institute of Biological Products Coronavirus Vaccine Based... by Zeyue Yu, Yudong Wang, Jianhui Sun, Xiaotong Zheng, Liyu Hao, Yurong Deng, Jianliang Li, Zongyuan Li, Zhongchao Shan, Weidong Li, Yuling Qiao, Ruili Huo, Yibai Xiong, Hairu Huo, Hui Li, Longfei Lin, Hanhui Huang, Guimin Liu, Aoao Wang, Hongmei Li, Luqi Huang

“…<b>Background</b>: Yiqi Zengmian (YQZM) functions as an immunopotentiator by enhancing both cellular and humoral immunity. …”
Get full text

The divergent outcome of IL-4Rα signalling on Foxp3 T regulatory cells in listeriosis and tuberculosis by Julius E. Chia, Julius E. Chia, Robert P. Rousseau, Robert P. Rousseau, Mumin Ozturk, Mumin Ozturk, Sibongiseni K. L. Poswayo, Sibongiseni K. L. Poswayo, Rodney Lucas, Frank Brombacher, Frank Brombacher, Frank Brombacher, Suraj P. Parihar, Suraj P. Parihar, Suraj P. Parihar, Suraj P. Parihar

“…In contrast to L. monocytogenes, Foxp3creIL-4Rα-/lox mice displayed similar bacterial burdens, lung pathology and survival during Mycobacterium tuberculosis (M. tuberculosis) infection, despite increased T cell numbers and IFN-γ, TNF and IL-17 production.ConclusionOur results demonstrated that the diminished IL-4Rα signalling on Foxp3+ T regulatory cells resulted in a loss of their functionality, leading to survival benefits in listeriosis but not in tuberculosis.…”
Get full text

Integration of transcriptome and metabolome reveals tolerance mechanism to high salinity stress in Crassostrea hongkongensis by Yixin Zhang, Qiwen Liang, Feifei Yu, Jiexiong Zhu, Jianfang Gui, Jinji Lin, Yishan Lu, Haiyuan Tao

“…A total of 232 differentially expressed genes (DEGs) and 968 DEGs were identified after salinity stress for 6 h and 48 h, respectively. …”
Get full text

Robust in vitro assay for analyzing the neutralization activity of serum specimens against hepatitis B virus by Ya-Li Zhang, Ying Gao, Jia-Li Cao, Jing-Hua Zhao, Tian-Ying Zhang, Chuan-Lai Yang, Hua-Long Xiong, Ying-Bin Wang, Shan-Hai Ou, Tong Cheng, Chang-Rong Chen, Quan Yuan, Ning-Shao Xia

“…However, little is known about the association between the qAnti-HBs determined by immunoassays and the neutralization activity (NAT) derived from functional assays. We developed an in vitro assay for direct measurement of the NAT of human sera. …”
Get full text

Deciphering the mechanisms for preferential tolerance of Escherichia coli BL21 to Cd(II) over Cu(II) and Ni(II): A combined physiological, biochemical, and multiomics perspective by Jackson Nkoh Nkoh, Ting Ye, Chenjing Shang, Chunyuan Li, Jianguang Tu, Sihui Li, Zuping Wu, Pengyu Chen, Quaid Hussain, Seraphine Nkie Esemu

“…Environmental pollution severely affects ecological functions/health, and nondegradable pollutants such as heavy metals (HMs) cause significant damage to living organisms. …”
Get full text

Monocyte-derived APCs are central to the response of PD1 checkpoint blockade and provide a therapeutic target for combination therapy by Louis Boon, Matheus H W Crommentuijn, Sjoerd T T Schetters, Laura J W Kruijssen, Yvette van Kooyk, Ernesto Rodriguez, Jan Van den Bossche, Joke M M Den Haan

“…It is still unclear how these APCs develop and function before and during αPD1 ICB or how they are associated with tumor rejection.Methods Here, we used B16 mouse melanoma and MC38 colorectal carcinoma tumor models, which show differential responses to αPD1 ICB. …”
Get full text

Beta-hydroxy-beta-methylbutyrate (HMB) improves daily activity and whole-body protein metabolism in Duchenne muscular dystrophy dogs: a pilot study by Peter P. Nghiem, Alexis M. Rutledge, Kyle Tehas, Corine Kaderli, Meredith Poling, Sidney Arnim, Vitaliy Dernov, Celine van Sas, Macie L. Mackey, Gabriella A. M. ten Have, Mariëlle P. K. J. Engelen, Nicolaas E. P. Deutz

“…We tracked daily activity with an activity monitoring device and measured plasma creatine kinase and standard clinical biochemistry panels to monitor muscle and organ function. To calculate whole body and intracellular amino acid production, we administered in the postabsorptive state an IV stable isotope solution containing 20 amino acid tracers. …”
Get full text

Intestinal TLR4 deletion exacerbates acute pancreatitis through gut microbiota dysbiosis and Paneth cells deficiency by Mei Qi-Xiang, Fu Yang, Huang Ze-Hua, Yin Nuo-Ming, Wang Rui-Long, Xu Bin-Qiang, Fan Jun-Jie, Huang Chun-Lan, Zeng Yue

“…Pancreatic injury and intestinal barrier function were evaluated in wild-type and intestinal epithelial TLR4 knockout (TLR4ΔIEC) mice. …”
Get full text

THE EFFICACY OF THERAPEUTIC MEASURES IN PATIENTS WITH GENERALIZED PERIODONTITIS ON THE BACKGROUND OF TYPE 1 DIABETES MELLITUS WITH CARDIOMYOPATHY ON SEPARATE PARAMETERS OF THE ORAL... by T. D. Zabolotnyj, O. I. Kordiyak, R. Y. Shkrebnyuk

“…The study proved the adequacy of the proposed treatment and preventive scheme in patients of the main group, which is confirmed by the improvement of endothelial function of the vessels of the periodontium, which is expressed by increasing the concentrations of nitrogen metabolites on the background of decrease of endothelin-1 in the oral liquid in this group of patients, in the absence of positive dynamics in the control group, where treatment was performed according to standard methods. …”
Get full text

Selenomethionine alleviates OTA-induced kidney injury in broilers by modulating ferroptosis by Wenqi Tian, Ruiwen Fan, Shuqin Zhou, Tao Liu, Yongdong Wang, Yuhang Sun, Miao Long, Shuhua Yang, Peng Li

“…Utilizing transmission electron microscopy, qRT-PCR, and immunoblotting techniques, the research will analyze pathological changes in broiler kidneys, examine ultrastructural alterations, and evaluate gene/protein expressions of renal function indicators, ferroptosis biomarkers, inflammatory-related parameters, and Nrf2-GPX4 signaling pathway components. …”
Get full text

Features of liver damage in patients with coronavirus disease (COVID-19) with pneumonia in relation to indicators of inflammation taking into account oxygen dependence by O. V. Riabokon, I. O. Kuliesh

“…The purpose of the work is to analyze the biochemical indicators of liver function in relation to indicators of inflammation in patients with coronavirus disease (COVID-19) with pneumonia, taking into account the development of oxygen dependence. …”
Get full text

Diagnostic value of serum levels of interleukin-9,interleukin-18 and interleukin-32 in patients with multiple myeloma by Zhuang Ni-la, Zhan Wen-li, Mo Tai-jin, Li Xiao-gan, Zeng Xiang-xing, Wang Qiu-xiang

“…Objective To explore the serum expressions of interleukin-9（IL-9）,interleukin-18（IL-18）and interleukin-32（IL-32）in patients with multiple myeloma（MM）and examine their values in predicting renal injury.Methods Seventy-eight MM patients from January 2015 to December 2019 were divided into phase I（n=15）,phase II（n=26）and phase III（n=37）according to the International Staging System.Based upon renal function, they were divided into renal injury group（n=28）and renal non-injury group（n=50）.Another 50 normal subjects in the same period were selected as control group.The serum levels of IL-9,IL-18,IL-32 and β₂-microglobulin（β₂-MG）were detected in each group.Receiver operating characteristic（ROC）curve was utilized for examining the values of serum levels of IL-9,IL-18,IL-32 and β₂-MG in the diagnosis of renal injury.Pearson’s correlation analysis was utilized for examining the correlation between serum levels of IL-9,IL-18,IL-32 and β₂-MG.Results The serum levels of IL-9[（63.15±18.36）ng/L vs（22.78±7.15）ng/L],IL-18[（580.64±70.35）ng/L vs（174.30±26.82）ng/L],IL-32[（817.62±112.36）ng/L vs（260.85±34.70）ng/L]and β₂-MG]（6.50±2.52）mg/L vs（1.66±0.58）mg/L]were significantly higher in MM group than those in control group（P<0.001）.With the advancing of ISS stage, the serum levels of IL-9,IL-18,IL-32 and β₂-MG spiked in MM patients（P<0.001）.The serum levels of IL-9[（91.26±19.42）ng/L vs（45.30±14.20）ng/L],IL-18[（812.46±95.2）ng/L vs（407.28±53.60）ng/L],IL-32[（1130.85±154.70）ng/L vs（604.20±71.38） ng/L]and β₂-MG[（10.18±4.75）mg/L vs（3.15±1.06） mg/L]were significantly higher in renal injury group than those in renal non-injury group（P<0.001）.ROC curve analysis revealed that the area under the curve（0.963,95%CI:0.905-0.997）of IL-9,IL-18,IL-32 and β₂-MG combined diagnosis of renal injury in MM was the largest and its sensitivity and specificity were 98.6% and 81.2%.Correlation analysis showed that serum levels of IL-9,IL-18,IL-32 and β₂-MG were positively correlated in MM patients（P<0.001）.Conclusion The serum levels of IL-9,IL-18 and IL-32 are significantly elevated in MM patients.Correlated with disease stage and renal injury, combine detection of β₂-MG has an excellent value in the diagnosis of renal injury in MM patients.…”
Get full text

Ginkgo Biloba Extract Reduces Cardiac and Brain Inflammation in Rats Fed a HFD and Exposed to Chronic Mental Stress through NF-κB Inhibition by Lijun Zhang, Guo Li, Shuhui Tao, Pengyan Xia, Naveed Chaudhry, Shawn Kaura, Sara Saymuah Stone, Meiyan Liu

“…Sucrose preference was established for the assessment of depressive behaviors. The heart function was evaluated by echocardiography. The rats were terminated at the end of the 10th or 13th week. …”
Get full text

Research on knowledge, attitude and practice (KAP) regarding dengue infection: a community-based study in rural Cox's Bazar by Saima Bibi

“…Descriptive statistics were expressed as frequency (percentage) and mean (±standard deviation; SD) for categorical and continuous data, respectively. …”
Get full text

Water use and crop coefficient of subsurface drip-irrigated lettuce in Central Arizona Demanda hídrica e coeficiente de cultura da alface irrigada por gotejamento sub-superficial n... by Aureo S. de Oliveira, Edward C. Martin, Donald C. Slack, Edward J. Pegelow, Allen D. Folta

“…The lysimeters were weighed periodically and the computation of the water balance revealed an average water use of 117 mm. Basal crop Kc was expressed as a function of cumulative growing degree days following a multiple linear regression procedure in which the data were fitted with a Fourier sine series model with up to six coefficients. …”
Get full text

OXA β-lactamases from Acinetobacter spp. are membrane bound and secreted into outer membrane vesicles by Lucia Capodimonte, Fernando Teixeira Pinto Meireles, Guillermo Bahr, Robert A. Bonomo, Matteo Dal Peraro, Carolina López, Alejandro J. Vila

“…Outer membrane vesicles (OMVs) from A. baumannii cells expressing OXA-23 and OXA-24/40 contain these enzymes in their active form. …”
Get full text