Functional architecture of pancreatic islets identifies a population of first responder cells that drive the first-phase calcium response. by Vira Kravets, JaeAnn M Dwulet, Wolfgang E Schleicher, David J Hodson, Anna M Davis, Laura Pyle, Robert A Piscopio, Maura Sticco-Ivins, Richard K P Benninger

“…Insulin-secreting β-cells are functionally heterogeneous. Whether there exist cells driving the first-phase calcium response in individual islets, has not been examined. …”
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Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-α: Crosstalk with p38 Signaling. by Alejandro P Adam, Anthony M Lowery, Nina Martino, Hiba Alsaffar, Peter A Vincent

“…TNF-α-induced p38 signaling was required for the synergistic effect on barrier function, and activation of the p38 MAPK alone was also able to induce changes in permeability only in monolayers with active SFKs. …”
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EVALUATION OF SUBSOIL MASSIF STABILITY TAKING INTO ACCOUNT THE IRREGULARITY OF CONSTITUENT ROCKS ON THE BASIS OF A RANDOM SIZE AND FUNCTION MODEL by M. O. Allaev

“…The cost of activities and the likelihood of loss are functional stability factors. The arrived-at expression determines the optimal massif stability coefficient value, which will correspond with the minimum total cost of the exercise and with additional losses in the event of a landslide. …”
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A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis. by Ewa Bielczyk-Maczyńska, Jovana Serbanovic-Canic, Lauren Ferreira, Nicole Soranzo, Derek L Stemple, Willem H Ouwehand, Ana Cvejic

“…Only a quarter of those genes, however, are known regulators of hematopoiesis. To determine function of the remaining genes we performed a medium-throughput genetic screen in zebrafish using antisense morpholino oligonucleotides (MOs) to knock down protein expression, followed by histological analysis of selected genes using a wide panel of different hematopoietic markers. …”
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Extraction of coronary thrombus-derived exosomes from patients with acute myocardial infarction and its effect on the function of adventitial cells. by Youfu He, Bo Wang, Yu Qian, Debin Liu, Qiang Wu

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Histone Deacetylase Inhibitors Enhance CD4 T Cell Susceptibility to NK Cell Killing but Reduce NK Cell Function. by Matthew Pace, James Williams, Ayako Kurioka, Andrew B Gerry, Bent Jakobsen, Paul Klenerman, Nneka Nwokolo, Julie Fox, Sarah Fidler, John Frater, CHERUB Investigators

“…We found HDACi down-regulated HLA class I expression independently of HIV-1 Nef which, without significantly compromising CTL function, led to enhanced targeting by NK cells. …”
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Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis by Peiyu Zhang, Guoning Zhu, Chunjiao Zhang, Hongliang Zhu

“…The family member SlMAP65-1, which had the highest expression, was knocked out by CRISPR/Cas9. The tomato fruit of slmap65-1 loss of function lines showed an elongated morphology, and the data indicated that SlMAP65-1 is involved in fruit morphogenesis at the early fruit development stage. …”
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Deciphering the multifaceted role of double-stranded RNA sensor protein kinase R: pathophysiological function beyond the antiviral response by Jiyoon Chung, Yerim Lee, Jimin Yoon, Yoosik Kim

“…This review explores PKR function in various pathological and physiological contexts in the absence of viral stimuli. …”
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Transcriptional and Post-transcriptional Mechanisms Limit Heading Date 1 (Hd1) Function to Adapt Rice to High Latitudes. by Daniela Goretti, Damiano Martignago, Martina Landini, Vittoria Brambilla, Jorge Gómez-Ariza, Nerina Gnesutta, Francesca Galbiati, Silvio Collani, Hiroki Takagi, Ryohei Terauchi, Roberto Mantovani, Fabio Fornara

“…We mined genetic variation in a collection of rice varieties highly adapted to European regions and isolated distinct variants of the long day repressor HEADING DATE 1 (Hd1) that perturb its expression or protein function. Specific variants allowed us to define novel features of the photoperiodic flowering pathway. …”
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Possible regulatory roles of promoter g-quadruplexes in cardiac function-related genes - human TnIc as a model. by Wenhua Zhou, Kogularamanan Suntharalingam, Nigel J Brand, Paul J R Barton, Ramon Vilar, Liming Ying

“…Although the anti-cancer potential of G4s in oncogene promoters has been thoroughly investigated, the functions of promoter G4s in non-cancer-related genes are not well understood. …”
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Chemosensory protein 16 has an immune function and participates in host-pathogen interaction in Galleria mellonella infected with Pseudomonas entomophila by Jakub Kordaczuk, Michał Sułek, Paweł Mak, Alicja Frączek, Iwona Wojda

“…Taking into account its immune properties and putative ability to bind bacteria-derived compounds, the possible function of CSP16 in the host-pathogen interaction is discussed.…”
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Effects of Sildenafil on Cognitive Function Recovery and Neuronal Cell Death Protection after Transient Global Cerebral Ischemia in Gerbils by Yeon Hee Yu, Gun Woo Kim, Yu Ran Lee, Dae-Kyoon Park, Beomjong Song, Duk-Soo Kim

“…Additionally, we performed various behavioral tests, including the open-field test, novel object recognition, Barnes maze, Y-maze, and passive avoidance tests, to evaluate sildenafil’s effect on cognitive function impaired by ischemia. Overall, the results suggest that sildenafil may serve as a neuroprotective agent, potentially alleviating delayed neuronal cell death and improving cognitive function impaired by ischemia.…”
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Assessing individual head and neck squamous cell carcinoma patient response to therapy through integration of functional and genomic data by Daniel Bottomly, Chase Mathieson, Myles Vigoda, Sophia Jeng, Nathaniel Evans, Ashley Anderson, Aurora Blucher, Aletha Lesch, Christina Zheng, Ted Laderas, James Jacobs, Molly Kulesz-Martin, Shannon McWeeney

“…Taking advantage of a high throughput inhibitor assay and computational tools originally showing success in leukemia, we designed and employed HNSCC-specific inhibitor panels that capture the diversity of aberrational pathways in HNSCC to test viable cells derived from patients’ HNSCC tumors. This provides a functional context to the multi-omic readouts conducted on these samples (mutations, protein expression and copy number alterations). …”
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Mice Lacking beta2-Integrin Function Remain Glucose Tolerant in Spite of Insulin Resistance, Neutrophil Infiltration and Inflammation. by Paul J Meakin, Vicky L Morrison, Claire C Sneddon, Terhi Savinko, Liisa Uotila, Susan M Jalicy, Jennie L Gabriel, Li Kang, Michael L J Ashford, Susanna C Fagerholm

“…Beta2-integrins are important in leukocyte trafficking and function, and are regulated through the binding of cytoplasmic proteins, such as kindlin-3, to their intracellular domain. …”
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Extrafollicular CD4 T cell-derived IL-10 functions rapidly and transiently to support anti-Plasmodium humoral immunity. by Fionna A Surette, Jenna J Guthmiller, Lei Li, Alexandria J Sturtz, Rahul Vijay, Rosemary L Pope, Brandon L McClellan, Angela D Pack, Ryan A Zander, Peng Shao, Linda Yu-Ling Lan, Daniel Fernandez-Ruiz, William R Heath, Patrick C Wilson, Noah S Butler

“…Distinct from reports of virus infection, we found that IL-10 was expressed by conventional, Foxp3-negative effector CD4 T cells and functioned in a B cell-intrinsic manner only during the first 96 hours of Plasmodium infection to support humoral immunity. …”
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Attrition of T-cell functions and simultaneous upregulation of inhibitory markers correspond with the waning of BCG-induced protection against tuberculosis in mice. by Subhadra Nandakumar, Sunil Kannanganat, James E Posey, Rama Rao Amara, Suraj B Sable

“…The BCG vaccination-induced CD4(+) and CD8(+) T cells exhibited comparable response kinetics but distinct functional attributes in-terms of IFN-γ, IL-2 and TNF-α co-production and CD62L memory marker expression. …”
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Restoration of a functional antiviral immune response to chronic HBV infection by reducing viral antigen load: if not sufficient, is it necessary? by Sisi Yang, Wanjia Zeng, Jiming Zhang, Fengmin Lu, Jinhong Chang, Ju-Tao Guo

“…Although it is clear that removal of antigen stimulation alone is not sufficient to induce the functional recovery of exhausted T and B cells, accumulating evidence suggests that the reduction of viral antigen load appears to facilitate the therapeutic activation of functional antiviral immunity in chronic HBV carriers. …”
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Structural and functional study of YER067W, a new protein involved in yeast metabolism control and drug resistance. by Tatiana Domitrovic, Guennadi Kozlov, João Claudio Gonçalves Freire, Claudio Akio Masuda, Marcius da Silva Almeida, Mónica Montero-Lomeli, Georgia Correa Atella, Edna Matta-Camacho, Kalle Gehring, Eleonora Kurtenbach

“…As the majority of these ORFs have no homologs with characterized sequence or protein structure, traditional sequence-based approaches cannot be applied to deduce their biological function. Here, we characterize YER067W, a conserved gene of unknown function that is strongly induced in response to many stress conditions and repressed in drug resistant yeast strains. …”
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ANALYSIS OF THE FUNCTIONING OF A COMBINED ACTIVE FILTER OF A SEQUENTIAL TYPE WITH SELECTIVE LINKS FOR DIRECT CURRENT TRACTION SUBSTATION by Ya. V. Shcherbak, Yu. A. Semenenko

“…As a result of analytical studies the expression of the transfer function of the control system of the active filter of the series type with selective link has been obtained. …”
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Integrative multi-omics and machine learning reveal critical functions of proliferating cells in prognosis and personalized treatment of lung adenocarcinoma by Shun Wang, Dingtao Hu, Ruohuang Wang, Jie Huang, Baoqing Wang

“…High- and low-SPRS groups exhibited different biological functions and immune cell infiltration in the TIME. …”
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