An integrated physiology and proteomics analysis reveals the response of wheat grain to low temperature stress during booting by Anmin Zhang, Zihong Li, Qirui Zhou, Jiawen Zhao, Yan Zhao, Mengting Zhao, Shangyu Ma, Yonghui Fan, Zhenglai Huang, Wenjing Zhang

“…A total of 509 differentially expressed proteins (DEPs) were identified by proteomics analysis. …”
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Transcriptome analysis of insecticide resistance mechanisms in field populations of the bean flower thrips, Megalurothrips usitatus (Bagnall) by Hongyi Cao, Jiangjiang Yuan, Yanran Wan, Yingxi Tang, Xiaobin Zheng, Jing Wang, Kanghua Qian, Jiuming Feng, Sirui Chen, Youjun Zhang, Qingjun Wu

“…RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA) techniques validated key gene functions. …”
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Proteomic analysis reveals candidate molecules to mediate cortical pathology and identify possible biomarkers in an animal model of multiple sclerosis by Berenice Anabel Silva, María Celeste Leal, María Isabel Farias, Agustín Nava, Agustín Nava, Daniela Inés Galván, Elmer Fernandez, Elmer Fernandez, Fernando Juan Pitossi, Carina Cintia Ferrari

“…IntroductionMultiple Sclerosis (MS) is a complex neurodegenerative disease marked by recurring inflammatory episodes, demyelination, axonal damage, and subsequent loss of function. MS presents a wide range of clinical courses, with the progressive forms leading to irreversible neurological disability. …”
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Impaired peroxisomal beta-oxidation in microglia triggers oxidative stress and impacts neurons and oligodendrocytes by Ali Tawbeh, Catherine Gondcaille, Fatima-Ezzahra Saih, Quentin Raas, Damien Loichot, Yannick Hamon, Céline Keime, Alexandre Benani, Francesca Di Cara, Mustapha Cherkaoui-Malki, Pierre Andreoletti, Stéphane Savary

“…These findings demonstrate that peroxisomal impairments in microglia exacerbate inflammatory response and ROS/NO production, affecting the survival of neurons and oligodendrocytes, as well as neuronal morphology and function. This dysfunction might contribute to the early neurodegenerative events in X-ALD by triggering and sustaining neuroinflammatory cascades. …”
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Role of LncRNA in Trauma Susceptibility and Resilience to Post-Traumatic Stress Disorder (PTSD): A Pilot Study in the African American Population by Sadhukhan T, Rai N, Misiak-Christian M, Hipolito MMS, Sadhukhan S, Shelby M, Mejía Mondragón CI, Idowu A, Gondringer A, Kalejaiye A, Li J, Islam MN, Ressom HW, Nwulia EA

“…RNA sequencing was performed to facilitate lncRNA-based analysis, exploring differentially expressed lncRNAs among the specified groups.Results: Two lncRNAs, CYP1B1-AS1 and SLC7A11-AS1, known for their neuroprotective and anti-inflammatory functions, were significantly elevated in the PTSD group compared to the non-trauma-exposed controls. …”
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An “EVs-in-ECM” mimicking system orchestrates transcription and translation of RUNX1 for in-situ cartilage regeneration by Qi Cheng, Qianping Guo, Xiaoyu Zhang, Yuanchen Zhu, Chengyuan Liu, Huan Wang, Caihong Zhu, Li Ni, Bin Li, Huilin Yang

“…When GelMA loaded with KGN and TD liposomes, the hydrogel (KGN + TD@ GelMA) showed biological functions by the continuously controlled release of KGN and TD while maintaining its porous structure and mechanical strength, which enhanced the chondrogenesis of MSCs in one system. …”
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Isobavachalcone ameliorates Alzheimer disease pathology by autophagy-mediated clearance of amyloid beta and inhibition of NLRP3 inflammasome in primary astrocytes and 5x-FAD mice by Dilpreet Kour, Dilpreet Kour, Parul Khajuria, Parul Khajuria, Kuhu Sharma, Kuhu Sharma, Alpa Sharma, Alpa Sharma, Ankita Sharma, Ankita Sharma, Syed Mudassir Ali, Priya Wazir, P. Ramajayan, P. Ramajayan, Sanghapal D. Sawant, Sanghapal D. Sawant, Sanghapal D. Sawant, Utpal Nandi, Utpal Nandi, Zabeer Ahmed, Zabeer Ahmed, Ajay Kumar, Ajay Kumar

“…Two months of treatment of 5x-FAD mice with IBC at 25 and 50 mg/kg significantly improved cognitive functions, as evidenced by enhanced memory and motor performance in behavioral tests. …”
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IgG4-mediated M2 macrophage polarization in tertiary lymphoid structures of esophageal cancer: implications for immunosuppression by Hui Wang, Hui Wang, Jirui Li, Yinghai Wang, Yang Chen, Yang Chen, Weifeng Zhang, Xinyan Pan, Xinyan Pan, Chanjuan Su, Ziteng Li, Li Wang, Li Wang, Jiang Gu, Jiang Gu

“…Tertiary lymphoid structures (TLS) in the TME have important immune-related functions. This study aims to analyze the distribution characteristics of IgG4-expressing cells, regulatory T cells (Tregs), and M2-type macrophages as well as to elucidate the relationship between IgG4 and the polarization of M2 macrophages within TLS in esophageal cancer.ObjectTo elucidate the distribution of IgG4, Treg cells, and M2 macrophages in TLS and to assess the impact of IgG4 on macrophage polarization.MethodsEsophageal cancer tissue were analyzed with multiplex immunofluorescence to determine the spatial distribution and density of B cells, T cells, and their subtypes. …”
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1,4‐Dioxane Induces Epithelial‐Mesenchymal Transition and Carcinogenesis in an Nrf2‐Dependent Manner by Ziwei Wang, Chitra Thakur, Zhuoyue Bi, Yiran Qiu, Wenxuan Zhang, Haoyan Ji, Arjun K. Venkatesan, Sashank Cherukuri, Ke Jian Liu, John D. Haley, Xinwei Mao, Jaymie Meliker, Fei Chen

“…Collectively, our findings suggest that 1,4‐D is a human carcinogen, with its carcinogenicity largely dependent on Nrf2 activation, which orchestrates the biogenesis of EVs with EMT‐promoting functions.…”
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Integration of Untargeted Metabolomics, Network Pharmacology, Single-Cell RNA Sequencing, and Molecular Dynamics Simulation Reveals GOT1, CYP1A2, and CA2 as Potential Targets of Hu... by Tiegang Li, Zheng Yan, Mingxuan Zhou, Wenyi Zhao, Fang Zhang, Silin Lv, Yufang Hou, Zifan Zeng, Liu Yang, Yixin Zhou, Zengni Zhu, Xinyi Ren, Min Yang

“…ScRNA-seq analysis indicated that HQD may enhance the functionality of cytotoxic T cells, thereby reversing the immunosuppressive microenvironment. …”
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Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer. by Minsung Kim, Suhkneung Pyo, Chung Hyo Kang, Chong Ock Lee, Heung Kyoung Lee, Sang Un Choi, Chi Hoon Park

“…Folate receptor 1 (FOLR1) is overexpressed on the cell surface in over one-third of gastric cancer patients, but rarely is expressed in normal tissue. This makes FOLR1 a potential target for chimeric antigen receptor (CAR) T cell immunotherapy, although the function of FOLR1 has not been elucidated. …”
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DIPLOMATIC LANGUAGE EVOLUTION IN THE TEXTS OF INTERNATIONAL AGREEMENTS (based on Karabakh conflict settlement documents) by Shafag F. Ahmedova., Nazim S. Ibadov.

“…An indicative trend is the weakening of the semantics of obligation and the strengthening of semantically neutral forms, expressed in the practical rejection of deontic modality use and the infinitive in the function of the imperative mood, as well as in the increasing activity of performative verbs use in the passive voice and verbal nouns. …”
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Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation by Marilu Mestanza, Marilu Mestanza, Angel David Hernández-Amasifuen, Alexandra Jherina Pineda-Lázaro, Dennis Eriksson, Juan Carlos Guerrero-Abad, Juan Carlos Guerrero-Abad

“…This approach allows precise mutation on site-specific gene at the DNA level, obtaining desirable traits when its function is altered. The CRISPR/Cas system could be used as a transgene-free genome editing tool when the ribonucleoprotein (RNP) acts as a carrier to delivered the CRISPR/Cas components into the plant cell protoplasts, or when the tRNA-like sequence (TLS) motifs are fused to single-guide RNA (sgRNA) and Cas mRNA sequence and expressed in transgenic plants rootstock to produce “mobile” CRISPR/Cas components to upper tissue (scion). …”
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Effects of West Nile virus on behavioral and cognitive performance, cortical Aβ pathology, viral loads, and immune measures of middle-aged NL-G-F/E3 and NL-G-F/E4 mice by Abigail O’Niel, Christopher J. Parkins, Alexandra Pederson, Elizabeth Saltonstall, Emily Bunnell, Ria Aggarwal, Phoebe Sandholm, Kat Kessler, Henry F. Harrison, Jessica L. Smith, Alec J. Hirsch, Jacob Raber, Jacob Raber, Jacob Raber, Jacob Raber

“…Compared to E3, E4 is an AD risk factor.MethodsWe crossed knock-in (KI) mice expressing human amyloid precursor protein (APP) containing the dominant NL-G-F mutations with human apoE targeted replacement (TR) mice and used middle-aged NL-G-F/E3 and NL-G-F/E4 mice to assess the role of prior WNV (subtype Kunjin virus) (KUNV) exposure on hAPP/Aβ-induced behavioral alterations, cognitive injury, circadian body temperatures, viral loads, neuropathology, and transcript levels of four immune measures important in the detrimental effects of KUNV on brain function.ResultsKUNV affected physiological, behavioral, cognitive, amyloid pathology, viral load, and immune measures in middle aged NL-G-F mice in an apoE isoform-dependent fashion. …”
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Monoclonal antibody and B-cell epitope mapping of the VP7 protein in bluetongue virus by Xin-Bing Hu, Jing Xu, Yu Zhang, Fan-Hua Meng, Zhan-Cheng Tian, Gui-Quan Guan, Jian-Xun Luo, Hong Yin, Jun-Zheng Du

“…These specific mAbs and identified B-cell epitopes provided key insights into the structure and function of VP7, while facilitating the development of BTV diagnostics and the design of epitope-based vaccines.…”
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How Does Nature Create the Painting “Gradient Coloration of ‘Manicure Finger’ Grape”? Integrated Omics Unveil the Pigments Basis and Metabolism Networks of Its Formation by Shihan Bao, Jianing Li, Jiaqi Wang, Tian Lan, Mengyuan Wei, Xiangyu Sun, Yulin Fang, Tingting Ma

“…In ‘Manicure Finger’, eight anthocyanins including malvidin‐3‐O‐glucoside, peonidin‐3‐O‐glucoside, and cyanidin‐3‐O‐glucoside were identified as color‐presenting substances, while flavones and flavonols, especially kaempferol‐3‐O‐rutinoside and quercetin‐3‐O‐glucoside, functioned as co‐pigments. Spatial differences in their accumulation were substances basis for gradient coloration. …”
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Exosomal lncRNA Mir100hg from lung cancer stem cells activates H3K14 lactylation to enhance metastatic activity in non-stem lung cancer cells by Lei Shi, Bowen Li, Jiyu Tan, Ling Zhu, Sicheng Zhang, Yuhan Zhang, Meng Xiang, Jie Li, Yan Chen, Xue Han, Jiacheng Xie, Yao Tang, H. Rosie Xing, Jingyu Li, Jianyu Wang

“…Previous studies on the role of EVs in metastasis have been primarily focused on cancer cell-derived EVs in modulating the functions of stromal cells. However, whether cancer stem cells (CSCs) can alter the metastatic properties of non-CSC cells, and whether EV crosstalk can mediate such interaction, have not been demonstrated prior to this report. …”
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Phosphoglucomutase A-mediated metabolic adaptation is essential for antibiotic and disease persistence in Mycobacterium tuberculosis by Taruna Sharma, Shaifali Tyagi, Rahul Pal, Jayendrajyoti Kundu, Sonu Kumar Gupta, Vishawjeet Barik, Vaibhav Kumar Nain, Manitosh Pandey, Prabhanjan Dwivedi, Bhishma Narayan Panda, Yashwant Kumar, Ranjan Kumar Nanda, Samrat Chatterjee, Amit Kumar Pandey

“…These insights are crucial for developing novel, shorter, and more effective anti-tuberculosis strategies.IMPORTANCEThis study for the first time investigated the role of metabolic enzyme phosphoglucomutase A (pgmA) in Mycobacterium tuberculosis (Mtb), revealing its crucial functions as a toggle switch between biosynthesis and growth. …”
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Diabetes is causally associated with increased breast cancer mortality by inducing FIBCD1 to activate MCM5-mediated cell cycle arrest via modulating H3K27ac by Binbin Tan, Yang Liu, Qianqian Chen, Weijie Yang, Wenhan Yang, Kaiping Gao, Li Fu, Tiantian Zhang, Penglong Chen, Yongyi Huang, Yuting Wang, Guoqiang Zhang, Juan Xiong, Rihong Zhai

“…Functionally, upregulation of FIBCD1 promoted proliferation, migration, and invasion capacities of BC cells in a glucose level-dependent manner. …”
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Single-Cell RNA Transcriptomics and Multi-omics Analyses Reveal the Clinical Effects of Acupuncture on Methadone Reduction by Yiming Chen, Baochao Fan, Jingchun Zeng, Yutian Zou, Chenyang Tao, Chen Chen, Peiming Zhang, Jian Liang, Fangfang Qi, Hailin Tang, Liming Lu

“…Our findings revealed that acupuncture may activate the defense response to viruses, with altered immune cell functions in classical monocytes correlating with clinical responses to reduced methadone doses. …”
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