Addressing the pathophysiology of venous thrombosis and chronic kidney disease in sickle cell trait using a mouse model by Fatima Trebak, Mohammad O. Sako, Steven P. Grover, Karnsasin Seanoon, Izabela Pawlinski, Chatphatai Moonla, Michael W. Henderson, Vimal K. Derebail, Dougald M. Monroe, Patrick Ellsworth, Rafal Pawlinski, Malgorzata Kasztan, Nigel S. Key

“…We demonstrated that the harsh environment within the inner medulla induces RBC sickling in vitro and in vivo and is associated with kidney-related pathologies, including impaired urinary concentration, albuminuria, and declining renal function, closely mimicking those seen in human SCT. …”
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Machine Learning‐Based Radiomics in Malignancy Prediction of Pancreatic Cystic Lesions: Evidence from Cyst Fluid Multi‐Omics by Sihang Cheng, Ge Hu, Shenbo Zhang, Rui Lv, Limeng Sun, Zhe Zhang, Zhengyu Jin, Yanyan Wu, Chen Huang, Lu Ye, Yunlu Feng, Zhe‐Sheng Chen, Zhiwei Wang, Huadan Xue, Aiming Yang

“…This models can effectively and accurately predict the malignant potential of PCLs, with multi‐omics evidence suggesting the biological mechanisms involving secretion function and lipid metabolism underlying clinical‐radiomic models.…”
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Lack of the human choline transporter‐like protein SLC44A2 causes hearing impairment and a rare red blood phenotype by Bérengère Koehl, Cédric Vrignaud, Mahmoud Mikdar, Thankam S Nair, Lucy Yang, Seyve Landry, Guy Laiguillon, Claudine Giroux‐Lathuile, Sophie Anselme‐Martin, Hanane El Kenz, Olivier Hermine, Narla Mohandas, Jean Pierre Cartron, Yves Colin, Olivier Detante, Raphaël Marlu, Caroline Le Van Kim, Thomas E Carey, Slim Azouzi, Thierry Peyrard

“…Overall, our findings confirm the function of SLC44A2 in hearing preservation and provide new insights into the possible role of this protein in maintaining cerebrovascular homeostasis.…”
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Human saliva exerts strong type-dependent effects on adenovirus infectivity by Erwan Sallard, Wenli Zhang, Nikita Chilakamarri, Setareh Farzanehkari, Setareh Farzanehkari, Inga Marte Charlott Seuthe, Anja Ehrhardt, Malik Aydin, Malik Aydin

“…Loss-of-function experiments were conducted by immunoprecipitation or enzymatic digestion of specific saliva components to decipher related mechanisms.ResultsTemporal and inter-individual variability in saliva samples were observed, validating the use of a saliva pool to represent the population. …”
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CD47 antibody-armed oncolytic adenovirus promotes chimeric antigen receptor macrophage phagocytosis and antitumor immunity by Zhongbing Qi, Shichuan Hu, Jing Zhao, Xianglin Xu, Anliang Huang, Yu Qin, Yao Zhang, Qingzhe Yang, Jianchuan Hu, Chao Su, Ping Cheng

“…Macrophages, being a potentially effective therapeutic target, have led to the development of several therapeutic strategies due to their unique phagocytic function. This study aimed to develop an effective solid tumor immunotherapy strategy by combining CAR macrophages (CAR-Ms) targeting PD-L1 with CD47 antibody-armed oncolytic adenovirus (oAd-CD47). …”
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Celastrol reduces cisplatin-induced nephrotoxicity by downregulating SNORD3A level in kidney organoids derived from human iPSCs by Chongayng Shen, Qizheng Wang, Xun Ye, Yi Zhou, Huayang Xing, Chengjie Pan, Heying Li, Chunjie Wu, Mingliang You

“…In addition, RNA-seq analyses were performed to determine the mechanisms of celastrol function in the kidney.ResultsKidney organoids exposed to 50 µM cisplatin showed significantly increased cell death (only 0.37% cells with normal cell structure), whereas celastrol under 5 µM (56% cells with normal cell structure) showed significantly less nephrotoxicity than cisplatin. …”
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Archives and Health program for dementia: A pilot study of a non-pharmacological creative arts-based intervention protocol by Sara Uboldi, Lorenza Iannacci, Vanda Menon, Alessandro Bortolotti, Giulia Candeloro, Alessandro Crociata, Valeria Pica, Angelo Romagnoli, Maria Tartari, Andrea Fabbo, Alessandra Marasco, Pier Luigi Sacco

“…Introduction: The global rise in dementia prevalence necessitates innovative non-pharmacological interventions to enhance cognitive function and the quality of life of affected individuals. …”
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Physical Activity after Cardiac EventS (PACES): a group education programme with subsequent text message support designed to increase physical activity in individuals with diagnose... by Melanie J Davies, Kamlesh Khunti, Laura J Gray, Thomas Yates, Helen Dallosso, Samuel Seidu, Alex V Rowlands, Patrick J Highton, Louisa Y Herring, Sally Schreder, Emily J Smith, Ghazala Waheed, Ian Hudson

“…Secondary outcomes included anthropometric, physical function, cardiovascular, biochemical and patient-reported outcome measures. …”
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O2-self-supplying and glutathione-depleting scaffold for synergistic cancer therapy by Jiajia Zeng, Chongxian He, Xinna Bai, Cijun Shuai, Hao Pan

“…In summary, introducing the Ti3C2 nanosystem loaded with nanozyme into PLLA scaffold is a promising strategy to develop bone scaffold with multimodal anti-tumor function.…”
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Mass cytometry analysis reveals a cross-tissue immune landscape in Actinobacillus pleuropneumoniae-induced pneumonia by Yanyan Tian, Xuan Jiang, Chuntong Bao, Tamin Abdelaal, Dexi Chen, Wenjing Wang, Fengyang Li, Liancheng Lei, Na Li

“…Meanwhile, the specific functions of APP infection-related immune subsets were explored. …”
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Allergen-specific circulating CLA+ memory T cells stratify IL-22 response in atopic dermatitis skin by Irene García-Jiménez, Irene García-Jiménez, Lídia Sans-de San Nicolàs, Sandra Díez-Ribas, Laia Curto-Barredo, Marta Bertolín-Colilla, Ana Vivancos-Melenchón, Ignasi Figueras-Nart, Montserrat Bonfill-Ortí, Anna Ryzhkova, Marta Ferran, Tali Czarnowicki, Ramon M. Pujol, Luis F. Santamaria-Babí

“…IL22P showed an increased degree of epidermal thickness, overexpression of IL22 in lesional skin areas, elevated specific IgE levels against HDM and SEB in plasma, and a higher proinflammatory profile compared to IL22NP.ConclusionsThis is the first report showing that allergen-specific CLA+ T-cell-mediated IL-22 in vitro response functionally distinguish moderate-to-severe adult AD patients with specific clinical features and activated IL-22 pathway in their lesional skin, paving the way for the selection of patients that may benefit from IL-22-directed therapies.…”
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Disease-derived circulating extracellular vesicle preconditioning: A promising strategy for precision mesenchymal stem cell therapy by Ke Lv, Tian Wu, Shuyun Liu, Peng Lou, Pingya Zhou, Yizhuo Wang, Xiyue Zhou, Shu Zhang, Dan Du, Yanrong Lu, Meihua Wan, Jingping Liu

“…Circulating extracellular vesicles (EVs) from diseased states carry diverse pathological information and affect the functions of recipient cells. Based on this unique property, we report that disease-derived circulating EV (disease-EV) preconditioning is a potent strategy for precisely enhancing the tissue repair potency of MSCs in diverse disease models. …”
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Ephrin-B2 deletion in GABAergic neurons induces cognitive deficits associated with single-nucleus transcriptomic differences in the prefrontal cortex by Peijun Ju, Yu Fang, Siying Xiang, Weidi Wang, Ying Sun, Wei Wang, Yanli Ding, Chao Luo, Ping Jiang, Jianhua Chen

“…Comprehensive identification of cell-type-specific transcriptomic changes in EB2 knockout mice is expected to shed light on potential mechanisms associated with EB2 signaling in cognitive functions. Results Our study captures changes in cell populations in response to EB2 manipulation and reveals previously uncharacterized cell types (CPA6 + inhibitory neurons) in the mPFC. …”
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RsWRKY40 coordinates the cold stress response by integrating RsSPS1-mediated sucrose accumulation and the CBF-dependent pathway in radish (Raphanus sativus L.) by Sen Chen, Liang Xu, Yan Wang, Baozhen Mao, Xiaoli Zhang, Qiyu Song, Feng Cui, Yingbo Ma, Junhui Dong, Kai Wang, Hongyu Bi, Liwang Liu

“…Moreover, RsWRKY40 was identified as the upstream transcription activator of RsSPS1 by binding to its promoter. RsWRKY40 functioned in cambium activity and cold tolerance by modulating RsSPS1-mediated sucrose accumulation. …”
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Interplay between the cyclophilin homology domain of RANBP2 and MX2 regulates HIV-1 capsid dependencies on nucleoporins by Haley Flick, Ananya Venbakkam, Parmit K. Singh, Bailey Layish, Szu-Wei Huang, Rajalingam Radhakrishnan, Mamuka Kvaratskhelia, Alan N. Engelman, Melissa Kane

“…Nups also mediate the function of the antiviral protein myxovirus resistance 2 (MX2); however, determining the precise role of Nups in HIV infection has proved challenging due to the complex nature of the nuclear pore complex (NPC) and significant pleiotropic effects elicited by Nup depletion. …”
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Deciphering the role of metal ion transport-related genes in T2D pathogenesis and immune cell infiltration via scRNA-seq and machine learning by Zuhui Pu, Zuhui Pu, Tony Bowei Wang, Ying Lu, Ying Lu, Zijing Wu, Zijing Wu, Yuxian Chen, Ziqi Luo, Xinyu Wang, Lisha Mou, Lisha Mou

“…Twelve protein structures were predicted using AlphaFold 3, revealing potential functional conformations. We observed a strong correlation between hub RMITRGs and immune cells, and PPI network analysis revealed key interactions. …”
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Non-coding RNA: A key regulator in the Glutathione-GPX4 pathway of ferroptosis by Sadique Hussain, Gaurav Gupta, Moyad Shahwan, Pooja Bansal, Harpreet Kaur, Mahamedha Deorari, Kumud Pant, Haider Ali, Sachin Kumar Singh, Venkata Sita Rama Raju Allam, Keshav Raj Paudel, Kamal Dua, Vinoth Kumarasamy, Vetriselvan Subramaniyan

“…Specifically, we delve into the functions of miRNAs, lncRNAs, and circRNAs in regulating GPX4 expression and impacting cellular susceptibility to ferroptosis. …”
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Neuroprotective effects of essential oils in animal models of Alzheimer’s and Parkinson’s disease: a systematic review by Adrielle do Espírito Santos Macedo, Thayná Moraes Ferreira, Lane Viana Krejcová, Fernando Allan de Farias Rocha, Joyce Kelly R. da Silva, Laís Resque Russo Pedrosa, Bruno Duarte Gomes

“…Behavioral assessments showed consistent enhancements in memory, learning, and motor functions across studies. The systematic analysis provides compelling evidence for EOs’ neuroprotective efficacy, particularly in early-stage intervention. …”
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Distinct bone metabolic networks identified in Phospho1−/− mice vs. wild type mice using [18F]FDG total-body PET by Abigail F. Hellman, Paul S. Clegg, Colin Farquharson, José Luis Millán, Carlos J. Alcaide-Corral, Carlos J. Alcaide-Corral, Karla J. Suchacki, Karla J. Suchacki, Adriana A. S. Tavares, Adriana A. S. Tavares

“…The skeletal system provides a good model for applying network analysis to total-body PET, as bone serves many classical whole-body functions as well as being an endocrine regulator of metabolism. …”
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Illuminating the “black box” of complex suicide prevention interventions: towards a theory of implementation using the normalisation process theory by Sadhvi Krishnamoorthy, Gregory Armstrong, Sharna Mathieu, Victoria Ross, Kairi Kõlves

“…The Normalisation Process Theory translational coding manual was used to map data related to the primary and secondary constructs defined in the theory and its extensions.ResultsThree domains pertaining to implementation context, mechanisms, and outcomes (CMO) were explored. Participants expressed their agency by: using contextual influences to modify the design and plan for delivery; making adaptations to the form and function of interventions and characteristics of the implementation environment to suit intervention needs; and, leveraging the intervention environment to integrate the intervention into existing systems and practices. …”
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