Molecular analysis highlights TREM2 as a discriminating biomarker for patients suffering from pancreatic ductal adenocarcinoma by Dimitrios Papakonstantinou, Haiding Wang, Mohamed-Amine Bani, Kevin Mulder, Garett Dunsmore, Alice Boilève, Gérôme Jules-Clément, Leonardo Panunzi, Leslie Robert de Sousa, Carlos de la Calle Fabregat, Marc Deloger, Nicolas Signolle, Grégoire Gessain, Sergey I Nikolaev, Michel Ducreux, Antoine Hollebecque, Florent Ginhoux, Camille Blériot

“…This work highlights the critical role of tumor-associated macrophages in pancreatic cancer progression and lays the groundwork for further functional and translational studies.…”
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The lamprey habenula provides an extreme example for the temporal regulation of asymmetric development by Lucile Guichard, Ronan Lagadec, Léo Michel, Hélène Mayeur, Michaël Fuentès, Jordan Pain, Noah Heier, Quentin Rougemont, Maria Celina Rodicio, Antón Barreiro-Iglesias, Antón Barreiro-Iglesias, Patrick Blader, Michael Schubert, Sylvie Mazan

“…These data confirm the importance of lampreys to unravel the developmental logic underlying the recurrence and variation of habenular asymmetries in vertebrates and pave the way for future functional analyses.…”
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Polypyrrole-ferric phosphate-methotrexate nanoparticles enhance apoptosis/ferroptosis of M1 macrophages via autophagy blockage for rheumatoid arthritis treatment by Hui Liu, Haoyu Wan, Anbiao Zhang, Yi Ouyang, Xinya Lu, Mengyuan Wu, Ning Hu, Jianying Pan, Dong Guo, Zhong Alan Li, Denghui Xie

“…Furthermore, PO4 3− disrupts the normal function of lysosomes by pH disturbance, disabling the cytoprotective autophagy of M1 macrophages for enhanced anti-RA effects. …”
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Fat storage-inducing transmembrane (FIT or FITM) proteins are related to lipid phosphatase/phosphotransferase enzymes by Matthew J Hayes, Vineet Choudhary, Namrata Ojha, John JH Shin, Gil-Soo Han, George M. Carman, Christopher JR Loewen, William A Prinz, Timothy P Levine

“…At the molecular level, the sole relevant interaction is that FITMs directly bind to triacyglycerol and diacylglycerol, but how they function at the molecular level is not known. Saccharomyces cerevisiae has two FITM homologues: Scs3p and Yft2p. …”
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The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence by Bin XU, Zhe MA, Hong ZHOU, Hui-xing LIN, Hong-jie FAN

“…M35246 showed a deletion of 25 contiguous genes as well as a loss-of-function mutation in covS. Subsequently, a 25-gene-deleted strain (ΔPI), a covS-mutant strain (McovS), and relevant complementary strains were constructed and investigated. …”
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MOTIVATIONAL COMPONENT AS A SUCCESS TO ORGANIZE AN INDEPENDENT WORK OF STUDENTS AT THE DEPATMENT OF THERAPEUTIC DENTISTRY by Yu. V. Sidash

“…The above-mentioned conditions for the development of independence in the conditions of a credit-module system together with pedagogical means, forms and methods of teaching provide a developing personality function during educational activities. …”
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Modeling Interference for Diffusive Molecular Communication Under Different Biological Deployment by Suman Yadav, S. Pratap Singh, Bharat Gupta

“…In particular, analytical expressions for Probability Density Function (PDF) of the received power spectral density (PSD) under CBD and SBD are derived. …”
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Effects of sinomenine on PI3K/mTOR signaling pathway and mesangial hyperplasia in rats with adriamycin-induced nephropathy by Liu Yue, Li Peng-yuan, Zhang Qing-hua, Yan Dan

“…Objective To explore the effect of sinomenine on the signal pathway of phosphatidyl-3 kinase（PI3 K）/mammalian target of rapamycin（mTOR）and mesangial hyperplasia in renal tissue of rats with adriamycin-induced nephropathy and elucidate its protective mechanism.Methods A total of 50 SD rats were selected, 10 rats were randomly injected with normal saline via caudal vein as control group, the other 40 rats were injected with adriamycin 5 mg/kg via caudal vein for 7 days to prepare nephrotic syndrome（NS）model.After successful modeling, the rats were randomly divided into the groups of model（NS）,sinomenine low-dose（25 mg/kg）,medium-dose（50 mg/kg）and high-dose（100 mg/kg）（n=10 each）.Control and NS groups received normal saline through abdominal cavity and sinomenine groups were given corresponding doses of drugs once daily for 28 days.At 24 hours after the last administration, blood and urine samples of rats in each group were collected and rats sacrificed, renal tissue samples harvested, serum creatinine（Scr）and urea nitrogen（BUN）were measured by enzyme-linked immunosorbent assay（ELISA）;24-hour urine protein was detected by Coomassie brilliant blue staining; hematoxylin-eosin staining（HE）was utilized for analyzing the pathological changes of kidney and mesangial matrix index（M/G）;transmission electron microscopy for observing the thickening of glomerular basement membrane of kidney; Western blot for detecting the expressions of p-PI3 K/PI3 K,p-mTOR/mTOR,fibronectin（FN）and type IV collagen（COL4）in renal tissue.Results As compared with control group, there were pathological damages such as the increase of glomerular mesangial matrix, the expansion of renal tubule lumen and severe thickening of glomerular basement membrane.M/G and renal function indicators Scr, BUN,24-hour urine protein and renal tissue were elevated.The protein expressions of p-PI3 K,p-mTOR,FN and COL4 rose markedly in NS group（P<0.05）.As compared with NS group, sinomenine high/medium/low dose groups alleviated pathological damage such as glomerular basement membrane thickening, M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,expressions of mTOR,FN and COL4 proteins declined markedly in sinomenine low/medium/high-dose groups（P<0.05）.As compared with low-dose sinomenine group, glomerular basement membrane thickening was the least pathologically damaged and M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,p-mTOR,FN and COL4 declined markedly in sinomenine medium/high-dose group（P<0.05）.Conclusions Sinomenine may inhibit the activation of PI3 K/mTOR signaling pathway, reduce the proliferation of glomerular mesangial cells and delay the process of nephrotic syndrome.…”
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NUMERICAL ANALYSIS OF THE CONDITIONS FOR THE OCCURRENCE OF FREE CONVECTION OF DRYAIR IN THE ATMOSPHERE by J. L. Smerek, I. S. Afanasief, R. G. Zakinyan, A. A. Krupkin

“…In this paper, using the mathematical package Maple 2021, a general solution of the problem of determining the current function describing the motion of an air particle when a pressure disturbance occurs in the atmosphere, as well as the components of the speed of movement in the vertical plane, is obtained. …”
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Nose-to-brain delivery of targeted lipid nanoparticles as two-pronged β-amyloid nanoscavenger for Alzheimer's disease therapy by Yanyan Xu, Xiangtong Ye, Yanfeng Du, Wenqin Yang, Fan Tong, Wei Li, Qianqian Huang, Yongke Chen, Hanmei Li, Huile Gao, Weiwei Zhang

“…Herein, we present an innovative nose-to-brain delivery platform utilizing lactoferrin (Lf)-functionalized lipid nanoparticles (LNPs) co-encapsulating α-mangostin (α-M) and β-site APP cleaving enzyme 1 (BACE1) siRNA (siB). …”
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LncRNA-HMG incites colorectal cancer cells to chemoresistance via repressing p53-mediated ferroptosis by Zechang Xin, Chenyu Hu, Chunfeng Zhang, Ming Liu, Juan Li, Xiaoyan Sun, Yang Hu, Xiaofeng Liu, Kun Wang

“…Nevertheless, the precise functions of long non-coding RNAs (lncRNAs) in ROS metabolism and tumor drug-resistance remain elusive. …”
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Reversible immortalisation enables genetic correction of human muscle progenitors and engineering of next‐generation human artificial chromosomes for Duchenne muscular dystrophy by Sara Benedetti, Narumi Uno, Hidetoshi Hoshiya, Martina Ragazzi, Giulia Ferrari, Yasuhiro Kazuki, Louise Anne Moyle, Rossana Tonlorenzi, Angelo Lombardo, Soraya Chaouch, Vincent Mouly, Marc Moore, Linda Popplewell, Kanako Kazuki, Motonobu Katoh, Luigi Naldini, George Dickson, Graziella Messina, Mitsuo Oshimura, Giulio Cossu, Francesco Saverio Tedesco

“…Finally, we combined the aforementioned functions into a next‐generation HAC capable of delivering reversible immortalisation, complete genetic correction, additional dystrophin expression, inducible differentiation and controllable cell death. …”
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MCL‑1 safeguards activated hair follicle stem cells to enable adult hair regeneration by Hui San Chin, Jinming Cheng, Shih Han Hsu, Guo Guang Lum, Maria TK Zaldivia, Sarmilla Nelameham, Fusheng Guo, Keerthana Mallavarapu, Felicity C. Jackling, Jicheng Yang, Jonathan S. L. Tan, Prabha Sampath, Nick Barker, Gordon K. Smyth, Geoffrey J. Lindeman, Andreas Strasser, Jane E. Visvader, Yunshun Chen, Ting Chen, Nai Yang Fu

“…To investigate the role of MCL‑1, a BCL‑2 family protein with anti‑apoptotic and apoptosis‑unrelated functions, we delete Mcl‑1 within the skin epithelium using constitutive and inducible systems. …”
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Metformin inhibits the growth of SCLC cells by inducing autophagy and apoptosis via the suppression of EGFR and AKT signalling by Hong Xia, Xue-jiao Tai, Wang Cheng, Yi Wu, Dan He, Li-feng Wang, Hao Liu, Shen-yi Zhang, Yu-ting Sun, Hang-zhi Liu, Dan-dan Liu, Hu-zi Zhao, Fu-yun Ji, Xi-hua Li

“…CCK-8, EdU, colony formation, flow cytometry, immunofluorescence, molecular docking, western blotting, nude mouse transplanted tumour model, and immunohistochemistry experiments were conducted to analyse gene functions and the underlying mechanism involved. In vitro experiments demonstrated that metformin inhibited the growth of SCLC cells (H446, H526, H446/DDP and H526/DDP), which was confirmed in xenograft mouse models in vivo. …”
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Microfluidics-enabled polydopamine-coated selenium nanoparticles in hyaluronic hydrogel microspheres for targeted antioxidant and immunomodulatory therapy of ulcerative colitis by Ying Fang, Hongwei Yu, Xiaochen Feng, Haike Xia, Yunze Xu, Ranjith Kumar Kankala, Aizheng Chen, Chaoping Fu

“…Overall, this microfluidics-enabled, colon-targeted delivery system with integrated antioxidative and immunomodulatory functions offers a promising strategy for the non-invasive treatment of UC and other inflammation-associated gastrointestinal disorders.…”
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PpHSP20-26, a small heat shock protein, confers enhanced autotoxicity stress tolerance in peach by Wanqi Shen, Chunfa Zeng, Jingxian Sun, Jian Meng, Ping Yuan, Fanwen Bu, Kaijie Zhu, Junwei Liu, Guohuai Li

“…Nevertheless, little is known about the functions and the underlying physiological mechanisms of sHSPs under autotoxicity stress. …”
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Deubiquitylase USP52 Promotes Bladder Cancer Progression by Modulating Ferroptosis through Stabilizing SLC7A11/xCT by Jianmin Liu, Yongwen Luo, Siming Chen, Gang Wang, Wan Jin, Wenyu Jiang, Mingxing Li, Yejinpeng Wang, Jingtian Yu, Houyi Wei, Renjie Zhang, Fenfang Zhou, Lingao Ju, Yi Zhang, Yu Xiao, Kaiyu Qian, Xinghuan Wang

“…USP52/PAN2 is identified as a key regulator of ferroptosis in BLCA through an unbiased siRNA screen targeting 96 deubiquitylases (DUBs). Functionally, USP52 depletion impedes glutathione (GSH) synthesis by promoting xCT protein degradation, increasing lipid peroxidation and ferroptosis susceptibility, thus suppressing BLCA progression. …”
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Synergistic transcriptomic and metabolomic analyses in Zi geese ovaries with different clutch lengths by Shengjun Liu, Jiaxin Yin, Kexin Cong, Shan Yue, Yuanliang Zhang, Jinyan Sun, Xiaofang Ren, Ke Jiang, Yunuo Liu, Xiuhua Zhao

“…Transcriptomic analysis identified 424 differentially expressed genes (DEGs). Functional enrichment analysis revealed that these DEGs were mainly involved in neuroactive ligand-receptor interactions, ovarian steroidogenesis, and calcium signaling pathways. …”
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Generation of non-genetically modified, CAR-like, NK cells by Martine Pugnière, Bruno Robert, Pierre Martineau, Loïs Coënon, Jessy Présumey, Emilie Rigal, Hortense Courot, Caroline Multrier, Sara Zemiti, Jennifer Lambour, Marion de Toledo, Guillaume Bossis, Guillaume Cartron, Bénédicte Fauvel, Martin Villalba

“…We took advantage of the natural expression of the IgG Fc receptor CD16a (FcγRIIIa) to induce strong antigen-specific effector functions through antibody-dependent cell-mediated cytotoxicity (ADCC). …”
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m6A-methylated TAL1 exacerbates lipid accumulation in ethylene bisdithiocarbamate metabolite–induced anorectal malformations in rat fetuses via miR-205/LCOR signaling by Yifan Yao, Shuo Yang, Yuzuo Bai, Zhengwei Yuan, Zhonghua Yang

“…Western blotting and qRT-PCR were used to explore the biological functions of the indicators in HEK 293 T cells and samples from ETU-induced ARM fetal rat models. …”
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