Neuroprotective effects of intraperitoneally injected Mg alloy extracts on middle cerebral artery occluded mouse with reperfusion injury by Lanjing Wang, Yue Hu, Hongkang Zhang, Miaowen Jiang, Yang Zhang, Yumin Luo, Xunming Ji, Yufeng Zheng, Sijie Li, Ming Li

“…We explored the underlying mechanisms and demonstrated that the Mg2+ and H2 from WE43 extracts could perform anti-inflammatory and anti-oxidative function after MCAO in mice, by increasing the expressions of IL-4, TGF-β1, SOD1 and decreasing the expressions of IL-1β, CXCR2, MDA. …”
Get full text

VPAC1 and VPAC2 Receptor Heterozygosity Confers Distinct Biological Properties to BV2 Microglial Cells by Xin Ying Rachel Song, Margo Iris Jansen, Rubina Marzagalli, Giuseppe Musumeci, Velia D’Agata, Alessandro Castorina

“…Dysregulation of microglial function is implicated in the pathogenesis of various neurodegenerative diseases. …”
Get full text

Dual targeting of BCMA and SLAMF7 with the CARtein system: chimeric antigen receptors with intein-mediated splicing elicit specific T cell activation against multiple myeloma by Noelia Moares, Noelia Moares, Pablo Gonzalez-Garcia, Pablo Gonzalez-Garcia, Wenjie Yi-He, Wenjie Yi-He, Juan P. Muñoz-Miranda, Juan P. Muñoz-Miranda, Antonio Gabucio, Antonio Gabucio, Rosa Luna-Espejo, Javier Ocaña-Cuesta, Ricardo Fernandez-Cisnal, Cecilia M. Fernandez-Ponce, Cecilia M. Fernandez-Ponce, Francisco Garcia-Cozar, Francisco Garcia-Cozar

“…This strategy maintains an almost seamless CAR structure, preserving its overall integrity and functionality. The design of the intein-spliced CAR system (termed "CARtein") was further optimized through advanced protein structure prediction software.ResultsCells expressing the spliced CARtein constructs, engineered to target BCMA, SLAMF7, or both antigens simultaneously, demonstrated robust and highly specific activation in response to their respective antigens.DiscussionThese results suggest that the CARtein platform is a promising, versatile, and highly specific approach for the modular design and engineering of CARs, enabling multi-antigen targeting while maintaining structural and functional integrity. …”
Get full text

Evaluation of the Immunostimulant Effect of Microvesicles of <i>Lactobacillus acidophilus</i> Isolated from Wild Rats by Pamela I. Pérez-Martínez, Viridiana Gutiérrez-Espinosa, Christian Ávalos-Gómez, Mireya De la Garza-Amaya, Alejandro Vargas-Ruíz, Rosa I. Higuera-Piedrahita, Ernesto Marín-Flamand, Cristal D. Lonngi-Sosa, Francisco R. González-Díaz, Hugo Ramírez-Álvarez, Cynthia González-Ruíz

“…It is capable of releasing membrane microvesicles (MVs), whose primary functions include carrying and transmitting antigens to host tissues and modulating host defense responses. …”
Get full text

Dual stimuli-responsive prodrug co-delivery nanosystem of salicylic acid and bioavailable silicon for long-term immunity in plant by You Liang, Yuehong Du, Yuchen Song, Sijin Wang, Can Zhao, Zhiming Feng, Shimin Zuo, Fengping Yang, Ke Xu, Zhongyang Huo

“…In this study, a novel amidase- and redox-responsive codelivery nanosystem was developed by covalently linking salicylic acid (SA) to functionalized disulfide-doped mesoporous silica nanoparticles (MSNs-ss-NH2) for the efficient delivery of SA and bioavailable silicon concurrently. …”
Get full text

Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery by Yingying Wang, Yingying Wang, Baiyan Wang, Yangfan Xiao, Qingchun Cai, Junyue Xing, Hao Tang, Ruiqin Li, Hongtao Zhang, Hongtao Zhang, Hongtao Zhang, Hongtao Zhang

“…This work provides a practical method to construct a charge-regulated, hydrophobic-modified, and functionally modified polyethylenimine (PEI) with effective gene delivery and perfect transfection performance through a condensation reaction, named BA-PEI. …”
Get full text

The polyamine transporter ATP13A3 mediates difluoromethylornithine‐induced polyamine uptake in neuroblastoma by Mujahid Azfar, Weiman Gao, Chris Van den Haute, Lin Xiao, Mawar Karsa, Ruby Pandher, Ayu Karsa, Dayna Spurling, Emma Ronca, Angelika Bongers, Xinyi Guo, Chelsea Mayoh, Youri Fayt, Arthur Schoofs, Mark R. Burns, Steven H. L. Verhelst, Murray D. Norris, Michelle Haber, Peter Vangheluwe, Klaartje Somers

“…High‐risk neuroblastomas, often associated with MYCN protooncogene amplification, are addicted to polyamines, small polycations vital for cellular functioning. We have previously shown that neuroblastoma cells increase polyamine uptake when exposed to the polyamine biosynthesis inhibitor difluoromethylornithine (DFMO), and this mechanism is thought to limit the efficacy of the drug in clinical trials. …”
Get full text

Epigenetic memory of radiotherapy in dermal fibroblasts impairs wound repair capacity in cancer survivors by Xiaowei Bian, Minna Piipponen, Zhuang Liu, Lihua Luo, Jennifer Geara, Yongjian Chen, Traimate Sangsuwan, Monica Maselli, Candice Diaz, Connor A. Bain, Evelien Eenjes, Maria Genander, Michael Crichton, Jenna L. Cash, Louis Archambault, Siamak Haghdoost, Julie Fradette, Pehr Sommar, Martin Halle, Ning Xu Landén

“…Here we show that the RT+ skin has compromised healing capacity and fibroblast functions. Using ATAC-seq, we discover altered chromatin landscapes in RT+ fibroblasts, with THBS1 identified as a crucial epigenetically primed wound repair-related gene. …”
Get full text

TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology by Athanasia Stoupa, Frédéric Adam, Dulanjalee Kariyawasam, Catherine Strassel, Sanjay Gawade, Gabor Szinnai, Alexandre Kauskot, Dominique Lasne, Carsten Janke, Kathiresan Natarajan, Alain Schmitt, Christine Bole‐Feysot, Patrick Nitschke, Juliane Léger, Fabienne Jabot‐Hanin, Frédéric Tores, Anita Michel, Arnold Munnich, Claude Besmond, Raphaël Scharfmann, François Lanza, Delphine Borgel, Michel Polak, Aurore Carré

“…All three TUBB1 mutations lead to non‐functional α/β‐tubulin dimers that cannot be incorporated into microtubules. …”
Get full text

Beyond structural domains: the emerging roles of PDLIM2 in cellular signaling and cancer progression by Yulian Shi, Yulian Shi, Yulian Shi, Yulian Shi, Tingyu Fan, Tingyu Fan, Tingyu Fan, Tingyu Fan, Yuchao Yang, Yuchao Yang, Yuchao Yang, Yuchao Yang, Jiaxuan Liu, Jiaxuan Liu, Jiaxuan Liu, Jiaxuan Liu, Jun Ouyang, Jun Ouyang, Jun Ouyang, Jun Ouyang, Jingxing Dai, Jingxing Dai, Jingxing Dai, Jingxing Dai

“…PDLIM2, also known as mystique or SLIM, is localized in the nucleus and cytoplasm and functions as a cytoskeleton-associated protein that facilitates binding of other proteins to the cytoskeleton. …”
Get full text

miRNA-34a-5p inhibits chicken myoblasts proliferation and differentiation via NOTCH1 inhibition by Xuanze Ling, Qifan Wang, Jin Zhang, Honghong Zhang, Liqi Zhang, Chuwen Zhuo, Tao Zhang, Genxi Zhang

“…Characteristic analysis revealed that the structures and functions of miR-34a-5p from 14 species were similar. …”
Get full text

Eimeria tenella aspartyl protease is identified as a potential TLR15 ligand and activates macrophages and dendritic cells in chickens by Chen Chen, Zhichao Men, Xianglin Pu, Mingmin Lu, Lixin Xu, Ruofeng Yan, Xiangrui Li, Xiaokai Song

“…However, the avian-specific TLR15 has unclear immunological functions, including its specific ligands and role in anti-coccidia immunity. …”
Get full text

Inositol polyphosphate multikinase regulates Th1 and Th17 cell differentiation by controlling Akt-mTOR signaling by Chae Min Yuk, Sehoon Hong, Dongeon Kim, Mingyo Kim, Hyun-Woo Jeong, Seung Ju Park, Hyungyu Min, Wooseob Kim, Jongbu Lim, Hyo Dam Kim, Sang-Gyu Kim, Rho Hyun Seong, Seyun Kim, Seung-Hyo Lee

“…Mechanistically, IPMK functions as a phosphatidylinositol 3-kinase to regulate phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3) production, promoting T cell activation and effector functions. …”
Get full text

Development of PVTX-405 as a potent and highly selective molecular glue degrader of IKZF2 for cancer immunotherapy by Zhixiang Chen, Harshil Dhruv, Xuqing Zhang, Rohan Kalyan Rej, Longchuan Bai, Donna McEachern, Paul Kirchhoff, Rakesh Nagilla, Larry J. Jolivette, Cory T. Rice, Peter Orth, Corey O. Strickland, E. Scott Priestley, Helai P. Mohammad, Meilin Wang, Bo Wen, Duxin Sun, Zhihua Sui, Shaomeng Wang

“…Abstract IKZF2 (Helios) is a transcription factor that is selectively expressed by Tregs and is essential for preserving the function and stability of Tregs in the tumor microenvironment (TME), where it suppresses the anti-tumor immune response. …”
Get full text

In-Depth Phenotyping of <i>PIGW</i>-Related Disease and Its Role in 17q12 Genomic Disorder by Agnese Feresin, Mathilde Lefebvre, Emilie Sjøstrøm, Caterina Zanus, Elisa Paccagnella, Irene Bruno, Erica Valencic, Anna Morgan, Alberto Tommasini, Christel Thauvin, Allan Bayat, Giorgia Girotto, Luciana Musante

“…We identify two novel genotypes and the first frameshift variant, supporting a loss-of-function pathogenic mechanism. By merging our cohort with patients documented in the literature, we deeply analyzed the clinical and genetic features of 16 patients with <i>PIGW</i>-related disorder, revealing a severe multisystemic condition deserving complex management and with uncertain long-term prognosis. …”
Get full text

Cloned airway basal progenitor cells to repair fibrotic lung through re-epithelialization by Yu Zhao, Yueqing Zhou, Weipan Zhang, Mingzhe Liu, Jun Duan, Xiaopeng Zhang, Qiwang Ma, Yujia Wang, Yuzhen Zhang, Zhongliang Guo, Ting Zhang, Wei Zuo

“…Mechanistically, the engrafted human progenitor cells do not function by differentiating into mature alveolar cells in mouse lung; instead, they differentiate into saccular cells expressing multiple tight junction proteins such as CLDN4, which help the lung to re-establish epithelial barriers. …”
Get full text

Perfluorinated compounds linked to central precocious puberty in girls during COVID-19: an untargeted metabolomics study by Haidan Li, Manfang Xie, Hailing Luo, Yuhua Cai, Li Liu, Hongai Li, Yuanping Hai, Yi Ren, Jing Xue, Xiaojie He, Xiaoyan Huang, Wei Xiang

“…KEGG pathway enrichment analysis suggested the involvement of multiple pathways in the CPP process regulated by these compounds.ConclusionsPerfluorinated compounds may promote CPP in girls by interfering with various pathways and affecting the hypothalamic-pituitary-gonadal axis function. This study highlighted the need for further research and public health measures to address environmental endocrine disruptors.…”
Get full text

A novel method for calculating number buildup factor in gamma-ray transmission measurements using narrow beam geometry by Chuong Huynh Dinh, Trang Le Thi Ngoc, Linh Nguyen Thi Truc, Nguyen Vo Hoang, Thanh Tran Thien

“…Furthermore, the slope of these linear relationships can be expressed as a product of mass density and a cubic polynomial function of the atomic number. …”
Get full text

Older surgical patients’ preferences for follow-up care after hospital discharge: A multi-method qualitative study into their underlying needs by Aafke J de Groot, Marike E de Boer, Elizabeth M Wattel, Cees MPM Hertogh, Marja FIA Depla

“…Background: Almost 30 % of older patients suffer from functional decline during hospital stay which often makes follow-up care decisions necessary. …”
Get full text

Markers of Inflammation and Hypofibrinolysis Are Associated with Cognitive Dysfunction and Motor Performances in Atrial Fibrillation Patients on Oral Anticoagulant Therapy: Insight... by Francesco Alfano, Francesca Cesari, Anna Maria Gori, Martina Berteotti, Emilia Salvadori, Betti Giusti, Alessia Bertelli, Filippo Fratini, Angela Rogolino, Benedetta Formelli, Francesca Pescini, Enrico Fainardi, Eleonora Barucci, Giulia Salti, Arianna Cavaliere, Andrea Ginestroni, Rossella Marcucci, Anna Poggesi

“…Results refer to 180 subjects who underwent a complete clinical, biohumoral, cognitive, and functional evaluation. <b>Results:</b> At multivariate logistic regression, Clot Lysis Time (CLT) and circulating levels of von Willebrand Factor (vWF) remained significantly associated with pathological performances at the Stroop test (expressed as execution time) [OR 95% CI 1.54 (1.02–2.35), <i>p</i> = 0.042 and 1.75 (1.08–2.82), <i>p</i> = 0.023, respectively]. …”
Get full text