A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, <i>Metopolophium dirhodum</i> (Walker) by Atsbha Gebreslasie Gebrekidan, Yong Zhang, Julian Chen

“…However, to verify the expression of these secretory genes and characterize the biological function of salivary proteins further investigation should be geared towards gene expression and functional analysis.…”
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CENPF overexpression in bladder cancer cells enhances proliferation, migration, invasion, and apoptosis by Ruixiang Song, Xin Chen, Zhensheng Zhang, Huiqing Wang, Jinshan Xu, Shuxiong Zeng, Wentao Zhang, Zhaoyang Sheng, Xudong Yao

“…This suggests that CENPF might be highly expressed in aggressive and invasive tumor cells. Subsequently, in vitro functional experiments also demonstrated that the siRNA interference of CENPF expression significantly weakened the proliferation, migration, invasion and apoptosis of BC cells, and the cells were arrested in the G2/S phase in Cell cycle. …”
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Mapping the human phosphatome on growth pathways by Francesca Sacco, Pier Federico Gherardini, Serena Paoluzi, Julio Saez‐Rodriguez, Manuela Helmer‐Citterich, Antonella Ragnini‐Wilson, Luisa Castagnoli, Gianni Cesareni

“…According to this strategy, genes are associated with a function of interest if the alteration of their expression perturbs the phenotypic readouts. …”
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Alterations to water quality and quantity elicit similar stream fish functional trait responses in three North American rivers by N.S. Santee, K.W. Conway, W.H. Nowlin, D. Smith, J.S. Perkin

“…The goal of this study was to conduct a multi-system assessment of fish functional trait responses to anthropogenic alteration of rivers. …”
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Systematic pan-cancer analysis identifies PKNOX1 as a potential prognostic and immunological biomarker and its functional validation by Kan Liu, Yongkang Xu, Ye Mao, Shumin Fu, Shenglan Huang, Jianbing Wu

“…In addition, the differential expression and function of PKNOX1 in HCC and breast cancer were explored via Western blotting and proliferation and migration-related experiments.ResultsPKNOX1 is significantly highly expressed in most tumor types and is significantly associated with poor patient prognosis and increased clinicopathological stage. …”
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miR-223-3p recover cognitive function in rats with hypertensive intracerebral hemorrhage by targeting NLRP3 by YU Mengqing, WU Jinbo, LAI Zhiyong, CHEN Zhongqiang

“…Methods The rat model of HICH was constructed to analyze changes in miR-223-3p expression and cognitive function. Subsequently， miR-223-3p was overexpressed or knocked down to investigate its effects on the expression of the inflammasome NLRP3 and cognitive function in rats. …”
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ESCRT may function as a tumor biomarker, transitioning from pan-cancer analysis to validation within breast cancer by Xiao-rui Chen, Xiao-rui Chen, Xue-ying Tan, Zong-liang Zhang, Jiang-shui Yuan, Wei-qing Song, Wei-qing Song

“…BRCA was used to explore its effects on tumor prognosis and therapy.MethodsThe data sets of Cancer Genome Atlas (TCGA), Genome-Tissue Expression Plan (GTEX), Human Protein Mapping (HPA), Gene Expression Omnibus (GEO), Clinical Proteomic Tumor Analysis Consortium (CPTAC), R software package, and bioinformatics methods were used to mine the potential carcinogenic effects of ESCRT, including the expression level, prognostic value, and immune value of ESCRT in various types of tumor tissues, and the potential function of ESCRT family genes was further verified in breast cancer.ResultsESCRT showed significant differential expression in various cancers, such as bladder urothelial carcinoma and liver, cervical, renal, esophageal, head, and neck cancers (P &lt;0.05). …”
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A variant form of receptor activator of nuclear factor-κB functions as an osteoprotegerin mimic in bone and cardiac development by Riko Kitazawa, Ryuma Haraguchi, Sohei Kitazawa

“…This study examined the regulatory mechanisms of vRANK expression in vitro and its physiological role in vivo using transgenic mice with forced vRANK expression in the monocyte-macrophage lineage and in a systemic manner. …”
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Nurr1 deficiency impairs autophagy-lysosomal function through GBA-dependent transcriptional regulation in Parkinson’s disease pathogenesis by Cheng Cheng, Cheng Cheng, Huijia Yang, Lulu Tian, Yang Ni, Congcong Jia, Weidong Le, Qingshan Wang

“…GBA was transcriptionally downregulated by Nurr1 and Nurr1 deficiency-triggered lysosomal dysfunction were attenuated by GBA overexpression in the Nurr1 knockdown cells.DiscussionOur study provided the first experimental evidence that Nurr1 deficiency induced lysosomal dysfunction by alkalizing the lumen of lysosomes and downregulating the key lysosomal protein (Lamp1, CTSD, and Lamp2) expression in vivo and in vitro. Defective lysosomal function compromised lysosomal mediated autophagic vesicle clearance. …”
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Integrated multi-omics analysis reveals the functional and prognostic significance of lactylation-related gene PRDX1 in breast cancer by Qinqing Wu, Qinqing Wu, Heng Cao, Jiangdong Jin, Dongxu Ma, Yixiao Niu, Yixiao Niu, Yanping Yu, Yanping Yu, Xiang Wang, Yiqin Xia

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Genome-wide analysis of the SPL gene family in Platanus × acerifolia and functional characterization of PaSPL8a in flowering by Xiyan Chen, Gehui Shi, Xu Yan, Jinghong Wu, Ting Peng, Manzhu Bao, Jiaqi Zhang

“…Among them, PaSPL8a, a Group III member, was differentially expressed in flowerless germplasm resources. Functional characterization demonstrated that PaSPL8a overexpression in Arabidopsis thaliana accelerated flowering and upregulated key flowering regulators. …”
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Functional analysis of conserved non-coding regions around the short stature hox gene (shox) in whole zebrafish embryos. by Emma J Kenyon, Gayle K McEwen, Heather Callaway, Greg Elgar

“…s CNEs were assayed for regulatory function in whole zebrafish embryos resulting in the identification of additional tissues under the regulatory control of these CNEs.…”
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Functional Analysis of <i>NdBCO-like4</i> Gene in Pigmentation of <i>Neocaridina denticulata sinensis</i> by Zhipeng Huo, Haifan Li, Guodong Wang, Tanjun Zhao

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Chromatin structure and 3D architecture define the differential functions of PU.1 regulatory elements in blood cell lineages by Kevin Qiu, Duc C. Vu, Leran Wang, Nicholas N. Nguyen, Anna K. Bookstaver, Katia Sol-Church, Hui Li, Thang N. Dinh, Adam N. Goldfarb, Daniel G. Tenen, Bon Q. Trinh

“…By correlating chromatin structure and 3D architecture with PU.1 expression in various lineages, we were able to attribute enhancer versus silencer functions to individual elements. …”
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The compensatory enrichment of sphingosine-1-phosphate on HDL in FSGS enhances the protective function of glomerular endothelial cells compared to MCD by Yunfeng Xiong, Qiuping Ye, Lifang Liu, Wanjun Lin, Yonggen Liao, Ruiyu Gao, Jiaming Xu, Xinyu Zhang, Ruoyan Chen, Sihui Chen, Qiaoling Chen, Lixin Wei

“…Incorporating S1P into HDL enhances glomerular endothelial cell function, suggesting that the S1P/S1PR pathway might offer a promising therapeutic avenue for FSGS.…”
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Effect of low-energy extracorporeal shock wave on vascular regeneration after spinal cord injury and the recovery of motor function by Wang L, Jiang Y, Jiang Z, Han L

“…The mRNA levels of VEGF and Flt-1 and VEGF expression at 7 days after SCI in Group D were significantly higher than those in Group C (P&amp;lt;0.05).Conclusion: Low-energy ESWT promotes expression of VEGF, decreases secondary damage of nerve tissue, and improves recovery of motor function. …”
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Rapid transient production in plants by replicating and non-replicating vectors yields high quality functional anti-HIV antibody. by Frank Sainsbury, Markus Sack, Johannes Stadlmann, Heribert Quendler, Rainer Fischer, George P Lomonossoff

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Functional analysis of a novel homozygous missense IVD gene variant: a case report with dual genetic diagnoses by Yuying Zhu, Ke Wu, Hanying Wen

“…Our in vitro functional and computer simulation findings revealed that this variant was associated with haploinsufficiency, which resulted in dramatically reducing the formation of IVD protein due to unstable mutant protein and not a lack of mRNA expression.ConclusionThe boy was diagnosed with the dual genetic disorders of Prader–Willi syndrome and isovaleric acidemia. …”
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Exploring the functional and immune landscape of E-β thalassemia patients through RNA sequencing of peripheral blood mononuclear cells by Nibedita Mitra, Prosanto Chowdhury, Anupam Basu

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The Aryl Hydrocarbon Receptor (AhR) Is a Novel Gene Involved in Proper Physiological Functions of Pancreatic β-Cells by Shuhd Bin Eshaq, Jalal Taneera, Shabana Anjum, Abdul Khader Mohammed, Mohammad H. Semreen, Karem H. Alzoubi, Mohamed Eladl, Yasser Bustanji, Eman Abu-Gharbieh, Waseem El-Huneidi

“…Furthermore, AHR expression showed an inverse correlation with the expression of key β-cell functional genes, including insulin, PDX-1, MAFA, KCNJ11, and GCK. …”
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