Characterization of CsWRKY29 and CsWRKY37 transcription factors and their functional roles in cold tolerance of tea plant by Huijuan Zhao, Ali Inayat Mallano, Fangdong Li, Penghui Li, Qiong Wu, Yanli Wang, Yunze Li, Naveed Ahmad, Wei Tong, Yeyun Li, Zhengzhu Zhang, Enhua Xia

“…Additionally, the functional roles of two genes, CsWRKY29 and CsWRKY37, were examined under cold stress; and the silencing of these genes resulted in tea plant phenotypes susceptible to cold stress. …”
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The Synthetic Cannabinoids ADB-FUBINACA Modulate Mitochondrial Function and Dynamics at Biologically Relevant Concentrations During In Vitro Neurodifferentiation by R.F. Malheiro, H. Carmo, F. Carvalho, J.P. Silva

“…Thus, here we aimed to evaluate the effects of this SC on mitochondrial function and dynamics during in vitro neurodifferentiation. …”
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Effect of streptococcal arginine deiminase on the function of CD4⁺ and CD8⁺T lymphocytes by E. A. Starikova, J. T. Mammedova, A. Ozhiganova, A. M. Lebedeva, T. A. Leveshko, L. A. Burova, I. V. Kudryavtsev

“…The data were analyzed using Kruskal–Wallis criterion, followed by Mann–Whitney criterion for pairwise comparisons and expressed as median and interquartile ranges (Q0.25-Q0.75).When comparing the effects of supernatants from the parental and mutant streptococcal strains, which differed in expression of arginine deiminase gene, we have shown that the bacterial enzyme had no effect on the functions of inactive lymphocytes. …”
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Functional characterization of transcriptional enhancers in an Anopheles genetic locus controlling natural resistance to the malaria parasite, Plasmodium falciparum by Natalia Marta Zmarlak-Feher, Kathryn S. Taquet, Renée Zakhia, Adrien Pain, Emma Brito-Fravallo, Cameron E. Anderson, Kenneth D. Vernick, Christian Mitri, Michelle M. Riehle

“…Abstract Background Anopheles mosquitoes and the malaria parasites they transmit remain a significant global health problem. Most genomic and functional genomic studies of mosquitoes have focused on the protein-coding genome, and comparatively little is known about the importance of noncoding transcriptional enhancers in controlling their gene expression and phenotypic variation. …”
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Pubertal low dose exposure to benzophenone-3 (BP-3) alters murine mammary stem cell functions by Hui Gao, Xintong Yang, Pengge Pan, Xueli Liu, Yan Ma, Yadan Chen, Yunxin Liu, Yaqi Sun, Sinan Cao, Yuan Tian, Yanzhou Yang

“…Taken together, our data suggest that pubertal BP-3 exposure decreases the function of basal mammary stem cells such that they induce the abnormal development of mammary glands.…”
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Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L. by Yuanyuan Liu, Xingzhi Wei, Yiwei Liu, Yunshan Tang, Shulin Shen, Jie Xu, Lulu Chen, Cunmin Qu, Huiyan Zhao, Hai Du, Huafang Wan, Nengwen Yin, Ti Zhang

“…This study provides the first experimental validation of the catalytic functions of BAHD enzymes in <i>B. napus</i>, establishing a theoretical foundation for leveraging this gene family in genetic improvement to develop novel rapeseed cultivars with enhanced stress tolerance and yield.…”
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Ice recrystallization inhibitors enable efficient cryopreservation of induced pluripotent stem cells: A functional and transcriptomic analysis by Kathleen Mommaerts, Satoshi Okawa, Margaux Schmitt, Olga Kofanova, Tracey R. Turner, Robert N. Ben, Antonio Del Sol, William Mathieson, Jens C. Schwamborn, Jason P. Acker, Fay Betsou

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Functional Trait Responses of <i>Brasenia schreberi</i> to Water and Soil Conditions Reveal Its Endangered Status by Jingyu Yao, Zhenya Liu, Junbao Yu, Yun Zhang, Rui Xu, Jiahua Li, Yang Xu, Mei Sun

“…[Results] (1) The growth status of <i>B. schreberi</i> affects the expression of its functional traits. Compared with sporadic distribution, <i>B. schreberi</i> in continuous patches have significantly higher stomatal conductance, intercellular CO₂ concentration, transpiration rate, and vein density, while these plants have significantly smaller leaf area and perimeter. (2) Good water quality directly promotes photosynthetic, morphological, and structural traits. …”
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Designer polyQ fusion proteins sequester USP7/HDM2 for modulating P53 functionality by Xiang-Le Zhang, Hong-Wei Yue, Ya-Jun Liu, Jian-Yang Wang, Heng-Tong Duan, Yin-Hu Liu, Lei-Lei Jiang, Hong-Yu Hu

“…Direct sequestration of HDM2 by Atx793Q-N172-PMI (PMI: TSFAEYWNLLSP) remarkably reduces the protein level of soluble HDM2 and hence increases the P53 level, which consequently up-regulates expression of the downstream genes. The polyQ-fusion strategy is feasible to modulate the P53 stability and functionality, furnishing a therapeutic potential for cancers.…”
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The Molecular Cloning and Functional Analysis of the <i>FAD2</i> Gene in <i>Hippophe rhamnoids</i> L. by Di Cong, Chang Ni, Luwen Han, Jianlin Cheng, Wei An, Siyu An, Hongzhang Liu, Huijing Liu, Dan Yao, Yuqin Fu, Shuying Liu, Guoshuang Chen

“…Accordingly, two <i>FAD2</i> genes have been identified in seabuckthorn, which are classified differently and perform distinct functions. Both genes are located in the endoplasmic reticulum. …”
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UHT Cow’s Milk Supplementation Affects Cell Niches and Functions of the Gut–Brain Axis in BALB/c Mice by Felipe S. Lemos, Caio A. Prins, Ana M. B. Martinez, Raul Carpi-Santos, Arthur S. Neumann, Nathalia Meireles-da-Costa, Roberto Luisetto, Valeria de Mello-Coelho, Felipe L. Oliveira

“…Here, we investigate whether cow’s milk consumption regulates gut–brain axis functions and affects patterns of behaviors in BALB/c mice, previously described by present low sociability, significant stereotypes, and restricted interest features. …”
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Phenotypic and Functional Characterization of Mesenchymal Stem/Multipotent Stromal Cells from Decidua Basalis of Human Term Placenta by F. M. Abomaray, M. A. Al Jumah, K. O. Alsaad, D. Jawdat, A. Al Khaldi, A. S. AlAskar, S. Al Harthy, A. M. Al Subayyil, T. Khatlani, A. O. Alawad, A. Alkushi, B. Kalionis, M. H. Abumaree

“…DBMSCs were negative for hematopoietic and endothelial markers, costimulatory molecules, and HLA-DR. Functionally, DBMSCs differentiated into three mesenchymal lineages, proliferated, and migrated in response to a number of stimuli. …”
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Soluble Siglec-9 Improves Intestinal Barrier Function in a Mouse Model of Metabolic Dysfunction-Associated Steatohepatitis by Hisanori Muto, Fumitaka Mizuno, Takashi Honda, Shinya Yokoyama, Taku Tanaka, Kenta Yamamoto, Takanori Ito, Norihiro Imai, Yoji Ishizu, Kiyoshi Sakai, Hideharu Hibi, Masatoshi Ishigami, Hiroki Kawashima

“…<b>Methods</b>: We evaluated sSiglec-9 effects on intestinal barrier function using in vitro Caco-2 cell monolayers injured by TNF-α and IFN-γ. …”
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Emission pattern and anti-insect function of indole from tea plant (Camellia sinensis) attacked by tea geometrids by Jianlong Li, Guotai Jian, Jiajia Qian, Jinghua Xue, Chengshun Liu, Yongxia Jia, Bo Zhou, Jinchi Tang, Jie Yang, Lanting Zeng

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Relationship of handgrip strength, asymmetries, and calf circumference with functional capacity in individuals with intellectual disabilities: an age group analysis by Justine Mendoza-Puelma, Julio B. Melo, Gerson Ferrari, Paloma Ferrero-Hernández, Alexis Espinoza-Salinas, Pedro Valdivia-Moral, Antonio Castillo-Paredes, Emilio Jofré-Saldía, Claudio Farías-Valenzuela

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Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality by Marianna Zahurak, Ravi Varadhan, Ilias Christodoulou, Ruyan Rahnama, Jun Choe, Huilin Yang, Monika Kizerwetter, Christian Guaraca, Natalie Jordan Holl, Stamatia C Vorri, Megan Zinsky, Danielle G Jones, Nikol Garcia Espinoza, Yun-Huai Kuo, Jamie B. Spangler, Challice L. Bonifant

“…Here, we study the functional consequences of CAR binding affinity and targeted epitope on CAR-NK cell activation, cytolytic synapse formation, and antitumor activity.Methods We characterized NK-92 and primary NK cell populations expressing variant affinity AML-specific CARs containing single-chain variable fragments (scFvs, 26292 or 7G3) targeting two epitopes on CD123. 26292 affinity variants were discovered through directed evolution of an error-prone mutagenic library, while 7G3 affinity variants were previously reported. …”
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ClBBM and ClPLT2 function redundantly during both male and female gametophytes development in watermelon by Qiyan Liu, Dongfen Han, Jinfan Chen, Jie Wang, Denghu Cheng, Xiner Chen, Jiao Jiang, Shujuan Tian, Jiafa Wang, Man Liu, Haizheng Xiong, Li Yuan

“…Collectively, ClBBM and ClPLT2 were functionally redundant in regulating gametophyte development in watermelon, and their functions differ from their homologous genes in model plant Arabidopsis.…”
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Genome-wide joint meta-analysis of SNP and SNP-by-smoking interaction identifies novel loci for pulmonary function. by Dana B Hancock, María Soler Artigas, Sina A Gharib, Amanda Henry, Ani Manichaikul, Adaikalavan Ramasamy, Daan W Loth, Medea Imboden, Beate Koch, Wendy L McArdle, Albert V Smith, Joanna Smolonska, Akshay Sood, Wenbo Tang, Jemma B Wilk, Guangju Zhai, Jing Hua Zhao, Hugues Aschard, Kristin M Burkart, Ivan Curjuric, Mark Eijgelsheim, Paul Elliott, Xiangjun Gu, Tamara B Harris, Christer Janson, Georg Homuth, Pirro G Hysi, Jason Z Liu, Laura R Loehr, Kurt Lohman, Ruth J F Loos, Alisa K Manning, Kristin D Marciante, Ma'en Obeidat, Dirkje S Postma, Melinda C Aldrich, Guy G Brusselle, Ting-hsu Chen, Gudny Eiriksdottir, Nora Franceschini, Joachim Heinrich, Jerome I Rotter, Cisca Wijmenga, O Dale Williams, Amy R Bentley, Albert Hofman, Cathy C Laurie, Thomas Lumley, Alanna C Morrison, Bonnie R Joubert, Fernando Rivadeneira, David J Couper, Stephen B Kritchevsky, Yongmei Liu, Matthias Wjst, Louise V Wain, Judith M Vonk, André G Uitterlinden, Thierry Rochat, Stephen S Rich, Bruce M Psaty, George T O'Connor, Kari E North, Daniel B Mirel, Bernd Meibohm, Lenore J Launer, Kay-Tee Khaw, Anna-Liisa Hartikainen, Christopher J Hammond, Sven Gläser, Jonathan Marchini, Peter Kraft, Nicholas J Wareham, Henry Völzke, Bruno H C Stricker, Timothy D Spector, Nicole M Probst-Hensch, Deborah Jarvis, Marjo-Riitta Jarvelin, Susan R Heckbert, Vilmundur Gudnason, H Marike Boezen, R Graham Barr, Patricia A Cassano, David P Strachan, Myriam Fornage, Ian P Hall, Josée Dupuis, Martin D Tobin, Stephanie J London

“…Genome-wide association studies have identified numerous genetic loci for spirometic measures of pulmonary function, forced expiratory volume in one second (FEV(1)), and its ratio to forced vital capacity (FEV(1)/FVC). …”
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Combinatorial mutagenesis and fermentation optimization biotechnologies synergistically enhance monacolin K content in functional red yeast rice by Zhengyan Wu, Chan Zhang, Qing Liu, Song Zhang, Qi Yang, Jun Liu, Dong Lu, Xiang Zhou

“…IntroductionRed yeast rice (RYR) is produced through solid-state fermentation by Monascus genus. Its functional component, Monacolin K (MK), has the same structure as lovastatin and can effectively inhibit HMG-CoA reductase, thereby reducing serum cholesterol.MethodsA combinatorial mutagenesis strategy integrating atmospheric room-temperature plasma and heavy-ion radiation was employed to generate mutant strains. …”
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Single-cell analysis of peripheral blood and pleural effusion reveals functional diversity of γδ T cells in tuberculosis infection by Yanjun Feng, Yu Chen, Wanying Zhang, Xinghua Shen, Jinyu Yan, Lin Yao, Lijuan Zhang, Yayan Niu, Jianping Zhang, Peijun Tang, Chunhua Ling

“…Through in-depth analysis, we identified a group of Vδ2 cells exhibiting strong effector function and high expression of FCGR3A.DiscussionTherefore, exploring the mechanism of interaction between Vδ2 cells and Mtb, as well as understanding host immune regulation during Mtb infection, can not only enhance the understanding of the immune mechanism underlying TB but also provide new theoretical ideas. …”
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