Phenotypic and functional profiling of CD4 T cell compartment in distinct populations of healthy adults with different antigenic exposure. by Sophie Roetynck, Ally Olotu, Joan Simam, Kevin Marsh, Brigitta Stockinger, Britta Urban, Jean Langhorne

“…<h4>Background</h4>Multiparameter flow cytometry has revealed extensive phenotypic and functional heterogeneity of CD4 T cell responses in mice and humans, emphasizing the importance of assessing multiple aspects of the immune response in correlation with infection or vaccination outcome. …”
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HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation by Giuliana Mastropietro, Inés Tiscornia, Karen Perelmuter, Soledad Astrada, Mariela Bollati-Fogolín

“…Here, we describe the generation, characterization, and validation of human epithelial reporter cell lines for functional studies of NF-κB activation by different pro- and anti-inflammatory agents. …”
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Amplified Electrochemical Aptasensor for Sialic Acid Based on Carbon‐Cloth‐Supported Gold Nanodendrites and Functionalized Gold Nanoparticles by Yubing Lv, Prof. Yanli Zhou, Dr. Hui Dong, Lantao Liu, Prof. Guoliang Mao, Dr. Yintang Zhang, Prof. Maotian Xu

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Synaptogenic gene therapy with FGF22 improves circuit plasticity and functional recovery following spinal cord injury by Almir Aljović, Anne Jacobi, Maite Marcantoni, Fritz Kagerer, Kristina Loy, Arek Kendirli, Jonas Bräutigam, Luca Fabbio, Valérie Van Steenbergen, Katarzyna Pleśniar, Martin Kerschensteiner, Florence M Bareyre

“…Here, we now explore whether and how targeted overexpression of FGF22 can be used to mitigate the severe functional consequences of SCI. By targeting FGF22 expression to either long propriospinal neurons, excitatory interneurons, or a broader population of interneurons, we establish that FGF22 can enhance neuronal rewiring both in a circuit‐specific and comprehensive way. …”
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Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels by Jose G. Munguia-Lopez, Sangeeth Pillai, Yuli Zhang, Amatzia Gantz, Dimitria B. Camasao, Showan N. Nazhat, Joseph M. Kinsella, Simon D. Tran

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Sporadic ALS induced pluripotent stem cell derived neurons reveal hallmarks of TDP-43 loss of function by Jeffrey D. Rothstein, Olivia Keeley, Caroline Warlick, Timothy M. Miller, Cindy V. Ly, Jonathan D. Glass, Alyssa N. Coyne

“…While studies using artificial TDP-43 depletion in neurons have revealed changes in gene expression and splicing, their relevance to actual patients remained unclear. …”
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PKM2 Facilitates Classical Swine Fever Virus Replication by Enhancing NS5B Polymerase Function by Mengzhao Song, Shanchuan Liu, Yan Luo, Tiantian Ji, Yanming Zhang, Wen Deng

“…This study investigates the role of pyruvate kinase M2 (PKM2), a glycolytic enzyme with non-canonical functions, in the replication of classical swine fever virus (CSFV). …”
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The Rhizophagus irregularis permease RiFTR1 functions without a ferroxidase partner for reductive iron transport by Elisabeth Tamayo, Víctor Manuel López-Lorca, Chaeeun Shim, Olga López-Castillo, Araceli G. Castillo, Natalia Requena, J. Philipp Benz, Nuria Ferrol

“…Yeast complementation assays demonstrated that RiMCO1 and RiMCO3 can function as ferroxidases, suggesting their involvement in the reductive Fe uptake pathway. …”
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Mitochondrial (Dys) Function in Inflammaging: Do MitomiRs Influence the Energetic, Oxidative, and Inflammatory Status of Senescent Cells? by Angelica Giuliani, Francesco Prattichizzo, Luigina Micolucci, Antonio Ceriello, Antonio Domenico Procopio, Maria Rita Rippo

“…Cellular senescence is also accompanied by a deep reshaping of microRNA expression and by the modulation of mitochondria activity, both master regulators of the SASP. …”
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Maps of open chromatin guide the functional follow-up of genome-wide association signals: application to hematological traits. by Dirk S Paul, James P Nisbet, Tsun-Po Yang, Stuart Meacham, Augusto Rendon, Katta Hautaviita, Jonna Tallila, Jacqui White, Marloes R Tijssen, Suthesh Sivapalaratnam, Hanneke Basart, Mieke D Trip, Cardiogenics Consortium, MuTHER Consortium, Berthold Göttgens, Nicole Soranzo, Willem H Ouwehand, Panos Deloukas

“…We showed that the two cell types exhibit distinct patterns of open chromatin and that cell-specific open chromatin can guide the finding of functional variants. We identified an open chromatin region at chromosome 7q22.3 in megakaryocytes but not erythroblasts, which harbors the common non-coding sequence variant rs342293 known to be associated with platelet volume and function. …”
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δ-Catenin is genetically and biologically associated with cortical cataract and future Alzheimer-related structural and functional brain changes. by Gyungah Jun, Juliet A Moncaster, Carolina Koutras, Sudha Seshadri, Jacqueline Buros, Ann C McKee, Georges Levesque, Philip A Wolf, Peter St George-Hyslop, Lee E Goldstein, Lindsay A Farrer

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Th17 Cells Exhibit Antitumor Effects in MDS Possibly through Augmenting Functions of CD8+ T Cells by Jing Li, Lanzhu Yue, Huaquan Wang, Chunyan Liu, Hui Liu, Jinglian Tao, Weiwei Qi, Yihao Wang, Wei Zhang, Rong Fu, Zonghong Shao

“…Here, we showed that both the percentage and the function of Th17 cells were elevated in low-risk MDS while being decreased in high-risk MDS. …”
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Structural, Functional and Inhibitory Annotations of Lumpy Skin Disease Virus Hypothetical Protein LSDV004: An In‐Silico Study by F. M. Yasir Hasib, Md. Shafiqul Islam

“…The tertiary structure predicted and refined using SWISS‐MODEL and GalaxyRefine respectively, > 90% of residues in the favoured region of the Ramachandran plot, according to PROCHECK validation. Functional prediction highlights structural similarities with the Swinepox virus C2 and suggests potential involvement in pathways such as negative regulation of gene expression, as indicated by gene ontology (GO) analysis using I‐TASSER. …”
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Not Just an Alternative Energy Source: Diverse Biological Functions of Ketone Bodies and Relevance of HMGCS2 to Health and Disease by Varshini V. Suresh, Sathish Sivaprakasam, Yangzom D. Bhutia, Puttur D. Prasad, Muthusamy Thangaraju, Vadivel Ganapathy

“…Loss-of-function mutations occur in HMGCS2 in humans, resulting in a severe metabolic disease. …”
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Adaptive genetics reveals constraints on protein structure/function by evolving E. coli under constant nutrient limitation by Katja Schwartz, Margie Kinnersley, Charles Ross Lindsey, Gavin Sherlock, Frank Rosenzweig

“…Whole-genome whole-population sequencing makes it possible to identify mutations arising in such populations, to use them to discern functional modules where adaptation occurs, and then map gene structure–function relationships. …”
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Mitochondrial subtypes in renal ischemia reperfusion injury guide delayed graft function and Long-Term graft prediction by Danni Hu, Zheng Wang

“…Abstract Background Ischemia reperfusion injury (IRI) in kidney transplantation (KTx) is closely associated with acute rejection, delayed graft function (DGF), and graft failure. Evidence highlights the significant correlation between mitochondrial dysfunction and IRI. …”
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Quercetin and Tranylcypromine Improve Memory, Behavioral Performance, and Cholinergic Function in Male Rats Subjected to Chronic Restraint Stress by Vitor Bastianello Mostardeiro, Charles Elias Assmann, Adriel Antonio Schirmann, Marcylene Vieira da Silveira, Bianca Vedoin Copês Rambo, Mairin Schott, Aline da Silva Pereira, Vanessa Valéria Miron, Heloiza Winck Soares, Larissa Varotto Dambrós, Sabrina Fontana Belinazo, Thamires Gelatti Vidal, Margarete Dulce Bagatini, Maria Rosa Chitolina Schetinger, Vera Maria Melchiors Morsch

“…Behavioral tasks were conducted to assess locomotor functions, memory, anhedonia, depression-like behaviors, and anxiety-like behaviors. …”
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Tumor cell‐specific inhibition of MYC function using small molecule inhibitors of the HUWE1 ubiquitin ligase by Stefanie Peter, Jennyfer Bultinck, Kevin Myant, Laura A Jaenicke, Susanne Walz, Judith Müller, Michael Gmachl, Matthias Treu, Guido Boehmelt, Carsten P Ade, Werner Schmitz, Armin Wiegering, Christoph Otto, Nikita Popov, Owen Sansom, Norbert Kraut, Martin Eilers

“…Abstract Deregulated expression of MYC is a driver of colorectal carcinogenesis, necessitating novel strategies to inhibit MYC function. …”
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Functional Analysis of Ectodysplasin-A Mutations in X-Linked Nonsyndromic Hypodontia and Possible Involvement of X-Chromosome Inactivation by Yuhua Pan, Ting Lu, Ling Peng, Qi Zeng, Xiangyu Huang, Xinchen Yao, Buling Wu, Fu Xiong

“…Candidate gene sequences were obtained by PCR amplification and Sanger sequencing. Functional analysis was conducted, and the pathogenesis associated with EDA mutations in hDPSCs was investigated to explore the impact of the identified mutation on the phenotype. …”
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Compensatory enhancement of orexinergic system functionality induced by amyloid-β protein: a neuroprotective response in Alzheimer’s disease by Chenyu Zhuang, Hengyu Yan, Jiayu Lu, Yifan Zhou, Yanqing Liu, Yanqing Liu, Guoshan Shi, Yan Li, Yan Li, Yan Li, Yan Li

“…Additionally, the excitability of orexinergic neurons was assessed by IHC targeting c-Fos expression. These methodologies evaluated the Aβ-induced neuroinflammation, orexinergic system functionality, and dexamethasone’s (Dex) effects on these processes.ResultsInjection of Aβ1-42 oligomer resulted in elevated levels of IL-6, TNF-α, and orexin-A in the hippocampus, as well as increased excitability of orexinergic neurons in the lateral hypothalamus (LH). …”
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