Evolution and Functional Diversity of GATA Transcription Factors in Filamentous Fungi: Structural Characteristics, Metabolic Regulation and Environmental Response by Danni Hu, Ruoyu Zhao, Yingxu Lin, Chunmiao Jiang

“…In addition, we explore their functions in the synthesis of secondary metabolites such as antibiotics (e.g., cephalosporins, penicillins) and organic acids (e.g., ganoderic acid, fumaric acid) in filamentous fungi. …”
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In Vivo Transplantation of Enteric Neural Crest Cells into Mouse Gut; Engraftment, Functional Integration and Long-Term Safety. by Julie E Cooper, Conor J McCann, Dipa Natarajan, Shanas Choudhury, Werend Boesmans, Jean-Marie Delalande, Pieter Vanden Berghe, Alan J Burns, Nikhil Thapar

“…Intestines were then assessed for ENCC integration and differentiation using immunohistochemistry, cell function using calcium imaging, and long-term safety using PCR to detect off-target YFP expression.…”
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Unveiling the Spatial Variation in Ecosystem Services Interactions and Their Drivers Within the National Key Ecological Function Zones, China by Tingjing Zhang, Quanqin Shao, Haibo Huang

“…We assessed and validated five key ES in the National Key Ecological Function Zones (NKEFZs) of China—net primary productivity (NPP), soil conservation (SC), sandstorm prevention (SP), water retention (WR), and biodiversity maintenance (BM). …”
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Nose-to-brain delivery of human muse cells enhances structural and functional recovery in the murine ischemic stroke model by Shusuke Yamamoto, Keitaro Shiraishi, Yoshihiro Kushida, Yo Oguma, Shohei Wakao, Mari Dezawa, Satoshi Kuroda

“…They can selectively home to the post-infarct area, replenish apoptotic neural cells by phagocytosis-induced differentiation, and enhance functional recovery. The effect of nose-to-brain delivery of Muse cells on cerebral infarct was examined. …”
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Monitoring of in vivo function of superparamagnetic iron oxide labelled murine dendritic cells during anti-tumour vaccination. by Richard Tavaré, Pervinder Sagoo, Gopal Varama, Yakup Tanriver, Alice Warely, Sandra S Diebold, Richard Southworth, Tobias Schaeffter, Robert I Lechler, Reza Razavi, Giovanna Lombardi, Gregory E D Mullen

“…Previous reports of superparamagnetic iron oxides (SPIOs) labelling of different cell types, including DCs, have indicated varying detrimental effects on cell viability, migration, differentiation and immune function. Here we describe an optimised labelling procedure using a short incubation time and low concentration of clinically used SPIO Endorem to successfully track murine DC migration in vivo using MRI in a mouse tumour model. …”
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Functional Analysis of Ectodysplasin-A Mutations in X-Linked Nonsyndromic Hypodontia and Possible Involvement of X-Chromosome Inactivation by Yuhua Pan, Ting Lu, Ling Peng, Qi Zeng, Xiangyu Huang, Xinchen Yao, Buling Wu, Fu Xiong

“…Candidate gene sequences were obtained by PCR amplification and Sanger sequencing. Functional analysis was conducted, and the pathogenesis associated with EDA mutations in hDPSCs was investigated to explore the impact of the identified mutation on the phenotype. …”
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A comparison of three methods in categorizing functional status to predict hospital readmission across post-acute care. by Chih-Ying Li, Amol Karmarkar, Yong-Fang Kuo, Hemalkumar B Mehta, Trudy Mallinson, Allen Haas, Amit Kumar, Kenneth J Ottenbacher

“…We used six differentiation and classification statistics to determine the optimal method of generating functional categories.…”
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Evenly Distributed Microporous Structure and E7 Peptide Functionalization Synergistically Accelerate Osteogenesis and Angiogenesis in Engineered Periosteum by Qihong Li, Chen Li, Jun Yan, Chunli Zhang, Yu Jiang, Xiantong Hu, Liwei Han, Li Li, Peng Wang, Lingzhou Zhao, Yantao Zhao

“…The E7 peptide (EPLQLKM), which exhibits a specific affinity for mesenchymal stem cells (MSCs), is beneficial for modulating cellular functions. In this study, a unique microporous structured carboxymethyl chitosan/sodium alginate membrane with a proper mass ratio is developed by the addition of Poloxam 407 （P407）, which is then functionalized with the E7 affinitive peptide. …”
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Functionalized biomimetic nanoparticles loaded with salvianolic acid B for synergistic targeted triple-negative breast cancer treatment by Nuo Cheng, Qianqian Zhou, Zongfang Jia, Yang Mu, Sheng Zhang, Lei Wang, Yunna Chen

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Generation and functional characterization of tuft cells in non-human primate pancreatic ducts through organoid culture systems by Kosuke Sakaguchi, Chiemi Kimura-Nakajima, Akihiko Inaba, Yoshiko Hatano, Hanako Ogawa, Yuichi Koshiishi, Keisuke Tanaka, Tatsuya Kometani, Makoto Ohmoto, Koji Sato, Hiroo Imai, Ken Iwatsuki

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Biomimetic integration of functionally controlled modular tissue building blocks for engineering 3D vascularized adipose tissue by Sangmin Lee, Jeongbok Lee, Hyunseok Kwon, Heungsoo Shin

“…Current efforts to engineer 3D vascularized adipose tissue in vitro typically involve co-culturing adipocytes and endothelial cells, but adipogenic differentiation often suppresses endothelial function. …”
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Changes in Ecological–Production–Social Functions in Karst Areas: Insight from Guizhou Province, South China Karst by Rong Zhao, Kangning Xiong, Anjun Lan, Qiwei Chen, Zhaojun Liu, Fangli Feng, Nana Yu

“…Therefore, this paper utilizes comprehensive evaluation methods, standard deviation classification, and coordination models to analyze the spatiotemporal changes in these functions of karst areas from 2000 to 2020. The results indicate that over the 20-year period, the ecological function, production function, and social function in karst areas have shown an annual growth trend with noticeable spatiotemporal differentiation. …”
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Functional aligned mesenchymal stem cell sheets fabricated using micropatterned thermo-responsive cell culture surfaces by Kenichi Nagase, Hasumi Kuramochi, David W. Grainger, Hironobu Takahashi

“…In addition, aligned MSC sheets maintained enhanced cell multi-potent differentiation capabilities. New, aligned MSC sheets might exhibit improved functional properties for cell sheet transplant therapies.…”
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Toll-like receptor 4 signaling activation domains promote CAR T cell function against solid tumors by Veronika Mikolič, Jelica Pantović-Žalig, Špela Malenšek, Matjaž Sever, Duško Lainšček, Roman Jerala

“…While the incorporation of CD28 or 4-1BB costimulatory signaling domains into CARs revolutionized immune responses, there is an exciting prospect of further enhancing CAR functionality. Here, we investigated the design of CD19 CARs enriched with distinct Toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88), or Toll/IL-1 domain-containing adaptor-inducing interferon (IFN)-β (TRIF) costimulatory domains. …”
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Integrated bioinformatics and functional studies identify CDK9 as a potential prognostic biomarker and therapeutic target in AML by Zhibin Xie, Yang Xia, Zhongyu Li, Mengmeng Zhang, Yuanyuan Tan, Yuqing Han, Meng Wang, Pingping Zhang, Jiajia Li

“…Results Bioinformatics analysis revealed significantly elevated CDK9 expression in AML samples, which correlated with poor patient prognosis. Functional enrichment analysis indicated that CDK9 is involved in key pathways related to cell proliferation, differentiation, and the tumor microenvironment. …”
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Engineered human B cells targeting tumor-associated antigens exhibit antigen presentation and antibody-mediated functions by Alexander Boucher, Courtney Anderson, Rochelle Hinman, Molly Kindschuh, Jeremy Fung, Tiansu Wang, Isabella Klooster, Elise Kim, Caroline Roth, Michael Vander Oever, Bakhmala Khan, Natalie Zelikson, Yaron Vagima, Huseyin Saribasak, Lisa Santry, Leah Natasha Klapper, Shmuel Hess, Jill Mooney, Débora Rosa Bublik, Haley Laken, Adi Barzel, Philip Borden, Cherylene Plewa, Ana Maria Chadbourne, Devin Bridgen, Alessio D. Nahmad, Alessio D. Nahmad

“…B cell engineering represents a promising therapeutic strategy that recapitulates adaptive immune functions, such as memory retention, antibody secretion and affinity maturation in murine models of viral infection. …”
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The Synthetic Cannabinoids ADB-FUBINACA Modulate Mitochondrial Function and Dynamics at Biologically Relevant Concentrations During In Vitro Neurodifferentiation by R.F. Malheiro, H. Carmo, F. Carvalho, J.P. Silva

“…Thus, here we aimed to evaluate the effects of this SC on mitochondrial function and dynamics during in vitro neurodifferentiation. …”
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Unique Function in Cancer Stemness and Prognostic Significance of <i>EMX2</i> in Esophageal Squamous Cell Carcinoma by Shoichi Fumoto, Keiji Tanimoto, Takuya Noguchi, Jun Hihara, Eiso Hiyama, Keiko Otani, Megu Ohtaki, Yutaka Shimada, Masahiko Nishiyama, Keiko Hiyama

“…Dysregulation of EMX2 expression has been implicated in various pathological conditions, including cancer, but the precise molecular mechanisms underlying EMX2 functions in cancer remain incompletely understood. …”
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Pubertal low dose exposure to benzophenone-3 (BP-3) alters murine mammary stem cell functions by Hui Gao, Xintong Yang, Pengge Pan, Xueli Liu, Yan Ma, Yadan Chen, Yunxin Liu, Yaqi Sun, Sinan Cao, Yuan Tian, Yanzhou Yang

“…Taken together, our data suggest that pubertal BP-3 exposure decreases the function of basal mammary stem cells such that they induce the abnormal development of mammary glands.…”
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Bioactive MXene hydrogel promotes structural and functional regeneration of skeletal muscle through improving autophagy and muscle innervation by Li Zhou, Haixia Zhuang, Xinyu Ye, Wei Yuan, Kai Wang, Donghan Hu, Xiangya Luo, Qiuyu Zhang

“…Importantly, FPGM could efficiently restore muscle function by improving muscle innervation, tibialis anterior compound muscle action potential amplitude and neuromuscular conduction. …”
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