Proteomic analysis identified proteins that are differentially expressed in the flavonoid and carotenoid biosynthetic pathways of Camellia Nitidissima flowers by Xing-Wen Zhou, Xiao-Xia Ye, Bao-Jian Ye, Shi-Hong Yan, Hai-Bin Hu, Qiu-Yuan Xu, Xiong Yao, He-Xia Liu, Bo Li, Yi-Qing Xie, Zhong-Jian Liu

“…Phytoene synthase and chalcone synthase exhibited extensive interactions with numerous other proteins and displayed high connectivity within the PPI networks, suggesting their pivotal biological functions in flavonoid and carotenoid biosynthesis. …”
Get full text

Explore the potential mechanism of Huachansu injection against osteosarcoma via metabolomics, network pharmacology and bioinformatics by Jingjing Meng, Xiangqi Zhang, Danfeng Xiang, Hanlu Liang, Shuai Zhao, Lingyan Xu, Jiao Yang, JunJun Chen, Jingxian Zhang, Yonglong Han

“…Abstract Aim Huachansu injection (HCSI) shows effective medicinal functions against osteosarcoma. This study aimed to reveal the underlying mechanisms of HCSI against osteosarcoma by integrating metabolomics, network pharmacology and bioinformatics. …”
Get full text

Lymphocyte activation gene 3 served as a potential prognostic and immunological biomarker across various cancer types: a clinical and pan‐cancer analysis by Yifan Liu, Yuntao Yao, Xinyue Yang, Maodong Wei, Bingnan Lu, Keqing Dong, Donghao Lyu, Yuanan Li, Wenbin Guan, Runzhi Huang, Guofeng Xu, Xiuwu Pan

“…Conclusion High LAG3 gene expression was an independent risk factor in kidney neoplasms. It also functioned as a biomarker for prognosis, TIME and immunotherapy efficacy in the pan‐cancer dimension.…”
Get full text

Arginine-Mediated Liver Immune Regulation and Antioxidant Defense in Largemouth Bass (<i>Micropterus salmoides</i>): Multi-Omics Insights into Metabolic Remodeling During <i>Nocard... by Yu-Long Sun, Shuai-Liang Zhang, Feng-Feng Zhou, Yuan-Xin Qian, Yang He, Run-Zhe Zhang, Fen Dong, Qiang Chen, Han-Ying Xu, Ji-Teng Wang, Yu-Ting Deng, Tao Han

“…The liver of fish is an essential metabolic organ that also serves an immune regulatory role. In this study, we constructed a model of largemouth bass (<i>Micropterus salmoides</i>) infected with <i>Nocardia seriolae</i> by injection to explore the immune and antioxidant functions of the liver. …”
Get full text

Barriers and facilitators to maternal death surveillance and response at a busy urban National Referral Hospital in Uganda [version 2; peer review: 2 approved] by Jolly Beyeza-Kashesya, Dan K.Kaye, Joseph Rujumba, Noah Kiwanuka, Moses Mukuru, Imelda Namagembe, Annettee Nakimuli, Josaphat Byamugisha, Ashley Moffett

“…Major facilitators were involving all health workers in the MDSR process, eliminate blame, strengthen leadership, implement recommendations from MDSR and functionalize lower health facilities (especially Health Centre -IVs). …”
Get full text

Enhancing neurological disease diagnostics: fusion of deep transfer learning with optimization algorithm for acute brain stroke prediction using facial images by Fadwa Alrowais, Mohammed Alqahtani, Jahangir Khan, Achraf Ben Miled, Da’ad Albalawneh, Abdulwhab Alkharashi, Samah Al Zanin, Radwa Marzouk

“…It contains a nervous illness, which can result from haemorrhage or ischemia of the brain veins, and regular mains to assorted motor and cognitive damages that cooperate with functionality. Screening for stroke comprises physical examination, history taking, and valuation of risk features like age or certain cardiovascular illnesses. …”
Get full text

Real‐Climatic Microcontroller‐in‐the‐Loop (RCMIL) Framework: A Novel, Rapid, and Cost‐Effective Approach for Verifying Photovoltaic Control Systems by Ambe Harrison, Idriss Dagal, Wulfran Fendzi Mbasso, Fritz Nguemo Kemdoum, Reagan Jean Jacques Molu, Hamid Belghiti, Pradeep Jangir

“…By harnessing real‐climatic functionalities with Microcontroller‐in‐the‐Loop (MIL) execution, the RCMIL provides a practical‐like environment, allowing the implementation of PV control systems in real‐world situations, mimicking the nonlinear and stochastic character of weather patterns in any geographic region. …”
Get full text

GPR65 is a novel immune biomarker and regulates the immune microenvironment in lung adenocarcinoma by Hanxu Zhou, Zhi Chen, Shuang Gao, Chaoqun Lian, Junjie Hu, Jin Lu, Lei Zhang

“…GPR65 is an extracellular pH-sensing G protein-coupled receptor and a glycosphingolipid receptor, which is engaged in the functions of regulating tumor immunity. However, the prognostic value of GPR65 and its relevance to immune infiltration in LUAD are unknown.MethodsThe proportion of immune, stromal and tumor cells in LUAD samples was assessed by ESTIMATE algorithm scores with RNA sequence data and clinical information from LUAD patients downloaded from The Cancer Genome Atlas (TCGA) database. …”
Get full text

Potential of a CO2-Responsive supramolecular drug-carrier system as a safer and more effective treatment for cancer by Enyew Alemayehu Bayle, Fasih Bintang Ilhami, Jem-Kun Chen, Chih-Chia Cheng

“…We combined carbon dioxide (CO2)-responsive cytosine-containing rhodamine 6G (Cy-R6G) as a hydrophobic anticancer agent with hydrogen-bonded cytosine-functionalized polyethylene glycol (Cy-PEG) as a hydrophilic supramolecular carrier to construct a CO2-responsive drug delivery system, with the aim of enhancing the responsiveness of the system to the tumor microenvironment and thus the overall effectiveness of anticancer therapy. …”
Get full text

Identification and prognostic analysis of propionate metabolism-related genes in head and neck squamous cell carcinoma by Shitong Zhou, Shitong Zhou, Yu Jiang, Panhui Xiong, Zhongwan Li, Lifeng Jia, Wei Yuan, Xiufu Liao, Xiang An, Jie Hu, Rui Luo, Hailan Mo, Hongyan Fang, Yucheng Yang

“…Recent studies have suggested that propionate metabolism-related genes (PMRGs) may play key roles in tumor progression and immune regulation, yet their functions in HNSCC remain unclear.MethodsTranscriptomic data from 502 HNSCC tumor samples and 44 normal tissue samples were obtained from the UCSC Xena database as the training set. …”
Get full text

Proteomic profiling of saliva reveals association of complement system with primary Sjögren's syndrome by Mingde Li, Yajun Qi, Guizhen Wang, Su Bu, Ming Chen, Jiahui Yu, Tianyang Luo, Lulu Meng, Anran Dai, Yong Zhou, Shuai Liu, Xingxing Huo

“…Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to functionally annotate the DEPs. The protein–protein interaction (PPI) network was constructed and the core proteins were identified with the STRING website and Cytoscape software. …”
Get full text

Single-cell RNA sequencing reveals the heterogeneity and regulatory modules of cell-specific RNA-binding proteins in patients with systemic lupus erythematosus by Sheng-Yan Lin, Yikai Yu, Daan Nie, Lili Yang, Yuxue Chen, Yu Chen, Cheng Wen, Zhipeng Zeng

“…Network modeling further uncovered coordinated interactions between JUN, HLA-A, and RAS events in immune genes, implicating their synergistic roles in modulating T cell effector functions. Conclusion: Our multi-omics integration identifies JUN and HLA-A as central coordinators of alternative splicing programs in SLE T cells. …”
Get full text

Interleukin-21 engineering enhances CD19-specific CAR-NK cell activity against B-cell lymphoma via enriched metabolic pathways by Bailin He, Hong Chen, Jiaxu Wu, Shiqiu Qiu, Qiusui Mai, Qing Zeng, Cong Wang, Shikai Deng, Zihong Cai, Xiaoli Liu, Li Xuan, Chengyao Li, Hongsheng Zhou, Qifa Liu, Na Xu

“…The phenotypic profile, transcriptomic characteristics, functionality and anti-tumor activity of CAR-21 NK cells and CAR-15 NK cells were compared. …”
Get full text

How Does Nature Create the Painting “Gradient Coloration of ‘Manicure Finger’ Grape”? Integrated Omics Unveil the Pigments Basis and Metabolism Networks of Its Formation by Shihan Bao, Jianing Li, Jiaqi Wang, Tian Lan, Mengyuan Wei, Xiangyu Sun, Yulin Fang, Tingting Ma

“…In ‘Manicure Finger’, eight anthocyanins including malvidin‐3‐O‐glucoside, peonidin‐3‐O‐glucoside, and cyanidin‐3‐O‐glucoside were identified as color‐presenting substances, while flavones and flavonols, especially kaempferol‐3‐O‐rutinoside and quercetin‐3‐O‐glucoside, functioned as co‐pigments. Spatial differences in their accumulation were substances basis for gradient coloration. …”
Get full text

Virtual human retina: Simulating neural signalling, degeneration, and responses to electrical stimulation by Keith Ly, Michael L. Italiano, Mohit N. Shivdasani, David Tsai, Jia-Yi Zhang, Chunhui Jiang, Nigel H. Lovell, Socrates Dokos, Tianruo Guo

“…Our study additionally highlights the importance of controlling inhibitory circuits in the retinal network to induce functionally relevant retinal activity. Conclusion: This study demonstrates the potential of this software package and highlights its utility as a valuable tool for engineers, scientists, and clinicians in the design and optimization of retinal stimulation devices for both research and educational applications.…”
Get full text

PediaTrac V.3.0 protocol: a prospective, longitudinal study of the development and validation of a web-based tool to measure and track infant and toddler development from birth thr... by H Gerry Taylor, Renee Lajiness-O'Neill, Seth Warschausky, Alissa Huth-Bocks, Judith Brooks, Angela Lukomski, Trivellore Eachambadi Raghunathan, Patricia Berglund, Angela D Staples, Laszlo Erdodi, Stephen Schilling

“…Participants also will complete legacy (ie, established) measures of development and caregiver health and functioning, used to provide evidence for construct (discriminant) validity. …”
Get full text

Investigating broadband filtering power amplifier using multi-mode resonator-based bandpass filter by Mohamed Boumalkha, Ahmed Gamal Abdellatif, Ahmed S.I. Amar, Mohammed Lahsaini, Amir Almslmany, Shuja Ansari, Mohammed Alammar, Mahmoud A. Shawky

“…By covering these widely adopted communication bands, the proposed FPA addresses the increasing demand for wideband, multifunctional devices in next-generation wireless networks, offering an effective balance among enhanced power efficiency, broadband functionality, high-quality filtering performance, and high selectivity.…”
Get full text

Development and test of digital twin simulation platform of “support-surrounding rock” based on dip effect by Panshi XIE, Yingyi ZHANG, Yongping WU, Yuqian DU, Hang YANG, Baofa HUANG, Weidian LIN, Leilei YI, Hui XU, Chun FENG

“…The integrated “support-surrounding rock” system comprising roof strata, supports, coal walls, and floor strata functions as a coordinated load-bearing structure, where variations in any component compromise overall stability and thereby exacerbate failures in other constituent elements. …”
Get full text

FMO2+ cancer-associated fibroblasts sensitize anti-PD-1 therapy in patients with hepatocellular carcinoma by Hui Li, Wenxin Xu, Qinghai Ye, Qiang Yu, Jialei Weng, Shaoqing Liu, Minghao Xu, Yinghao Shen, Hui-Chuan Sun, Yufei Zhao, Peiyi Xie, Mincheng Yu, Bugang Liang, Junbo Chen

“…Mouse orthotopic HCC models and a coculture system were constructed, and cytometry by time-of-flight analysis was performed to investigate the functions and mechanisms of specific CAFs in the immune context of HCC.Results We identified a distinct flavin-containing monooxygenase 2 (FMO2)+ CAF subset associated with a favorable response to anti-PD-1 therapy and better clinical outcomes. …”
Get full text

Identification of prognostic biomarkers related to epithelial-mesenchymal transition and anoikis in hepatocellular carcinoma using transcriptomics and single-cell sequencing by Maobing Wang, Lu Cheng, Lu Cheng, Lu Cheng, Lu Cheng, Kuo Qi, Kuo Qi, Kuo Qi, Kuo Qi, Haiping Wang, Haiping Wang, Haiping Wang, Haiping Wang, Xun Li, Xun Li, Xun Li, Xun Li

“…The risk model and nomogram were constructed based on biomarkers. Subsequently, the potential functions of biomarkers were explored through methods such as enrichment analysis and immune microenvironment analysis. …”
Get full text