Hepatocellular Metabolic Profile: Understanding Post-Thawing Metabolic Shift in Primary Hepatocytes In Vitro by Salvator Palmisano, Joshua D. Breidenbach, Brett R. Blackwell, Tara Harvey, Kes A. Luchini, M. Grace Thornhill, Erick S. LeBrun, Phillip Mach, Trevor Glaros, Emilio S. Rivera

“…Primary human hepatocytes (PHHs) are widely used as in vitro models for liver function and drug metabolism studies, yet their metabolic stability post-thawing remains an open question. …”
Get full text

Homeobox protein B6 and homeobox protein B8 control immune-cancer cell interactions in pancreatic cancer by Ludivine Bertonnier-Brouty, Sara Bsharat, Kavya Achanta, Jonas Andersson, Thanya Pranomphon, Tania Singh, Tuomas Kaprio, Jaana Hagström, Caj Haglund, Hanna Seppänen, Rashmi B. Prasad, Isabella Artner

“…Moreover, reduced HOXB6 and HOXB8 expression in pancreatic and lung adenocarcinoma cell lines affected transcription of immune response pathways which resulted in an increased sensitivity of cancer cells to anti-tumorigenic activities of macrophages suggesting that the HOXB6 and HOXB8 immune regulatory function is conserved in different cancer types. Additionally, naïve M0 macrophages exposed to HOXB8 deficient PDAC cells were unable to differentiate into tumor-associated macrophages, suggesting that HOXB8 promotes the transition of initial anti-tumor macrophage to a tumor-promoting macrophage phenotype in pancreatic cancer. …”
Get full text

Long-term mulched drip irrigation enhances cotton yield by improving soil quality, microbial community structure, and N conversion by Wenhao Li, Shuanglong Gao, Wenying Qu, Junfeng Li, Tehseen Javed, Shih-Hsin Ho, Zhenhua Wang

Get full text

Control of Clostridioides difficile virulence and physiology by the flagellin homeostasis checkpoint FliC-FliW-CsrA in the absence of motility by Duolong Zhu, Katherine J. Wozniak, Firas Midani, Shaohui Wang, Xingmin Sun, Robert A. Britton

“…Here, we show that clinically important, non-motile C. difficile strains have conserved FliC and regulatory partners FliW and CsrA, despite lacking the rest of the machinery to produce functional flagella. …”
Get full text

Methylation of p53-responsive oncosuppressive microRNA genes in hemoblastosis by E. N. Voropaeva, T. I. Pospelova, O. V. Berezina, M. I. Churkina, A. A. Gurazheva, V. N. Maksimov

“…The review examines the molecular and genetic characteristics of oncosuppressive micrornas functioning in normal hematopoiesis, the violation of expression of which is shown in the development of hemoblastoses, namely: miR-34a, miR-34b/c, miR-145, miR-143 and miR-203. …”
Get full text

The Impact of Flow Channel Structural Parameters on Both the Hydraulic Performance and Anticlogging Abilities of Variable Flow Emitters by Peining Niu, Yan Mo, Baolin Yao, Zongze Yang, Yanqun Zhang, Dequan Zhang

Get full text

Interferon-β and interleukin-6 exert opposing effects on Foxp3 acetylation to control regulatory T cell induction by Mehek Ningoo, Mehek Ningoo, Francisco Fueyo-González, Cayetana Gisbert-Vilanova, Cayetana Gisbert-Vilanova, Laura Espinar-Barranco, Laura Espinar-Barranco, Nada Marjanovic, Miguel Fribourg, Miguel Fribourg

“…Mechanistically, we demonstrate that IFNβ and IL-6 signal independently to promote opposing effects on the acetylation of Foxp3, an essential transcription factor governing Treg differentiation, stability, and function. We further show that this mechanism is conserved in both murine and human T cells, highlighting the broad relevance of this finding in immune regulation. …”
Get full text

Crystallographic and NMR Study of <i>Streptococcus pneumonia</i> LCP Protein Psr_Sp Indicate the Importance of Dynamics in Four Long Loops for Ligand Specificity by Tatyana Sandalova, Benedetta Maria Sala, Martin Moche, Hans-Gustaf Ljunggren, Evren Alici, Birgitta Henriques-Normark, Tatiana Agback, Dmitry Lesovoy, Peter Agback, Adnane Achour

“…Despite the low sequence homology with other LCP proteins, the Psr_Sp maintains the fold of the LCP domain, and the positions of the residues suggested to participate in the transferase function are conserved. The tunnel found in the Psr_Sp between the central β-sheet and three α-helices is wide enough to accommodate polyisoprenyl-teichoic acid. …”
Get full text

Structural basis for the human SENP5’s SUMO isoform discrimination by Lucía Sánchez-Alba, Li Ying, Matthew D. Maletic, Anna De Bolòs, Helena Borràs-Gas, Bing Liu, Nathalia Varejão, Virginia Amador, Monique P. C. Mulder, David Reverter

“…Abstract Post-translational SUMO modification is a widespread mechanism for regulating protein function within cells. In humans, SUMO-conjugated proteins are partially regulated by the deconjugating activity of six SENP family members. …”
Get full text

The Expression and Molecular Roles of MAMDC2 in MSS Colorectal Cancer with a High Tumor Stromal Ratio by Yiling Liu, Shengnan Qian, Jia Wei, Jianting He, Minghui Li, Xiaobing Gao, Hong Cai, Yiqing Wang, Yue Han, Tianyuan Tan, Minhui Yang

“…<b>Conclusions:</b> Our findings suggest MAMDC2 may function as a stromal-associated regulator in MSS colorectal cancer with a high tumor stromal ratio (TSR).…”
Get full text

Efficacy and safety of endoscopic ultrasonography‐guided radiofrequency ablation of small pancreatic neuroendocrine neoplasms: A prospective, pilot study by Kazuyuki Matsumoto, Daisuke Uchida, Yasuto Takeuchi, Hironari Kato, Yuki Fujii, Kei Harada, Nao Hattori, Ryosuke Sato, Taisuke Obata, Akihiro Matsumi, Kazuya Miyamoto, Shigeru Horiguchi, Koichiro Tsutsumi, Kazuya Yasui, Ryo Harada, Masakuni Fujii, Motoyuki Otsuka

“…Results Five patients with non‐functional PNENs (median age: 64 years; median tumor size: 10 mm) were treated. …”
Get full text

A fish-specific antimicrobial peptide MsPiscidin2 inactivates MSRV and confers protection in largemouth bass by Chenjie Fei, Chenjie Fei, Chenjie Fei, Ziwen Wang, Ziwen Wang, Yang Hu, Yang Hu, Yang Hu, Li Nie, Li Nie, Li Nie, Jiong Chen, Jiong Chen, Jiong Chen

“…Antimicrobial peptides (AMPs) represent an evolutionarily conserved component of innate immunity with broad-spectrum antimicrobial and antiviral activities. …”
Get full text

Responsiveness of the mini-balance evaluation systems test, dynamic gait index, Berg balance scale, and performance-oriented mobility assessment in parkinson’s disease by Maryam Mehdizadeh, Seyed-Mohammad Fereshtehnejad, Merrill R. Landers, Parvaneh Taghavi Azar Sharabiani, Mohsen Shati, Seyede Salehe Mortazavi, Sayed Amir Hasan Habibi, Korosh Mansoori, Mahsa Meimandi, Zahra Nodehi, Zakieh Sadat Saberi, Ghorban Taghizadeh

“…The intervention consisted of task-oriented exercises focused on motor re-learning to improve functional balance. Anchor and distribution methods were used to determine the MID (the receiver-operating characteristic(ROC)-based (MIDROC), the predictive modeling method (MIDpred), and the MIDpred-based method adjusted by the rate of improvement (MIDadj)) and MDC. …”
Get full text

Exosome Therapy in Stress Urinary Incontinence: A Comprehensive Literature Review by Manouchehr Nasrollahzadeh Saravi, Mahdi Mohseni, Iman Menbari Oskouie, Jafar Razavi, Ernesto Delgado Cidranes, Masoumeh Majidi Zolbin

“…Exosome therapy has the potential to enhance tissue regeneration, restore urethral function, and repair nerve and muscle damage, thereby reducing symptom burden and improving patients’ quality of life. …”
Get full text

Genome-wide identification of pyrabactin resistance 1-like (PYL) gene family under phytohormones and drought stresses in alfalfa (Medicago sativa) by Kun Wang, Jiao Cheng, Jing-Ru Chen, Yan-Yan Luo, Yu-Heng Yao, Li-Li Nan

“…MsPYL genes contains cis-acting elements that has functions in plant growth and development, hormone regulation, and stress response. …”
Get full text

miR-184, a downregulated ovary-elevated miRNA transcriptionally activated by SREBF2, exerts anti-apoptotic properties in ovarian granulosa cells through inducing SMAD3 expression by Baosen Shan, Yangan Huo, Zhennan Guo, Qiqi Li, Zengxiang Pan, Qifa Li, Xing Du

“…Moreover, the expression pattern, function, target, and regulatory mechanism of miR-184 among mammals are highly conserved and universal. …”
Get full text

The death of a neurotrauma trial lessons learned from the prematurely halted randomized evaluation of surgery in elderly with traumatic acute subdural hematoma (RESET-ASDH) trial by R.D. Singh, J.T.J.M. van Dijck, T.A. van Essen, H.P. Nix, R.J.G. Vreeburg, H.F. den Boogert, G.C.W. de Ruiter, B. Depreitere, W.C. Peul

“…Materials and methods: The RESET-ASDH was a multicenter randomized controlled trial (RCT) comparing functional outcome at 1 year after early surgery or an initial conservative treatment in elderly patients (≥65 years) with a traumatic ASDH. …”
Get full text

mgr-mir-228-regulated transthyretin-like protein in Meloidogyne graminicola suppresses ROS generation and enhances parasitism by Zhongling Tian, Yixiao Cai, Mingming Zhu, Liang Wang, Qiannan Liu, Qinghong Li, Xiaoyi Gao, Jingwu Zheng, Borong Lin, Kan Zhuo, Shaojie Han

“…These findings suggest that MgTTL1, specifically regulated by the newly identified miRNA mgr-mir-228, functions as a negative regulator of ROS-mediated plant immunity and plays a pivotal role in modulating plant susceptibility to nematode infections.…”
Get full text

Genome-Wide Analysis of the EIN3/EIL Transcription Factors in <i>Osmanthus fragrans</i> and Their Stress Response to Azacytidine (AZA) and Ethylene (ETH) Treatment by Dou Pan, Chun Xu, Wanlu Ma, Xinyi Zhu, Qiangjun Yu, Yingting Zhang, Jie Yang, Xiangling Zeng, Xuan Cai, Jingjing Zou

“…Strikingly, <i>OfEIL7</i> exhibited dual regulatory roles, correlating strongly with natural flowering progression, AZA-induced demethylation, and ETH responses. Functional divergence was observed in petal senescence, with <i>OfEIL2–5</i> and <i>OfEIL7–9</i> showing stage-specific and tissue-specific expression patterns. …”
Get full text

Shared transcriptional regulators and network rewiring identify therapeutic targets linking type 2 diabetes mellitus and hypertension by Claudia Desireé Norzagaray-Valenzuela, Claudia Desireé Norzagaray-Valenzuela, Marco Antonio Valdez-Flores, Josue Camberos-Barraza, Josue Camberos-Barraza, Alberto Kousuke De la Herrán-Arita, Juan Fidel Osuna-Ramos, Javier Magaña-Gómez, Carla Angulo-Rojo, Alma Marlene Guadrón-Llanos, Katia Aviña-Padilla, Loranda Calderón-Zamora

“…This study aimed to uncover shared transcriptional programs and disease-specific regulatory networks contributing to cardiometabolic dysfunction.MethodsWe systematically selected transcriptomic datasets and employed an integrative systems biology approach that combined differential gene expression analysis, co-expression network construction, protein-protein interaction mapping, transcription factor activity inference, and network rewiring analysis. Functional enrichment analyses were conducted to elucidate biological processes associated with disease-specific modules.ResultsWe identified distinct regulatory modules: ME3 in T2DM, enriched in metabolic stress response, intracellular trafficking, and inflammation, and ME7 in HTN, enriched in immune response and vascular remodeling. …”
Get full text