The effect of a complex of melatonin, aluminum oxide and polymethylsiloxane on the cellular composition of the mice spleen kept in round-the-clock lighting conditions by A. V. Shurlygina, S. V. Michurina, L. N. Rachkovskaya, A. E. Serykh, S. M. Miroshnichenko, E. E. Rachkovsky, M. A. Korolev, A. Yu. Letyagin

“…In this regard, it is relevant to search for opportunities to restore disturbed circadian rhythms and, especially, to correct immune dysfunctions that occur in these situations.The aim of this study was to examine the effect of a complex of melatonin, aluminum oxide and polymethylsiloxane on the lymphocytes of the spleen of mice kept under round-the-clock lighting.Materials and methods. Mice of the C57Bl/6J line were kept under round-the-clock lighting for 14 days, against which they were intragastrically injected with distilled water, aluminum oxide with polydimethylsiloxane, melatonin and a complex of melatonin, aluminum oxide and polymethylsiloxane (a new drug developed by the Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics SB RAS; Patent of Russian Federation No. 2577580, 2016), represented by a complex of porous material (aluminum oxide with polydimethylsiloxane) and melatonin, immobilized in the pores, from which it is gradually released in a liquid medium. …”
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Proteomic Characterization of Extracellular Vesicles from Human Neural Precursor Cells: A Promising Advanced Therapy for Neurodegenerative Diseases by Stricker PEF, de Oliveira NB, Mogharbel BF, Irioda AC, da Rosa NN, Lührs L, Saçaki CS, Munhoz da Rocha I, Alves LR, Poubel SB, Cardoso da Silva J, Carvalho PC, Fischer JSG, de Carvalho KAT

“…One such component released is extracellular vesicles containing proteins and genetic materials with immunomodulatory functions and facilitating cell-to-cell communication.Purpose: The study’s main objective was to characterize extracellular vesicles (EVs) from Human Neural Precursor Cells (hNPCs).Methods: Wharton’s Jelly mesenchymal stem cells (WJ-MSCs) were isolated by explant technique and characterized by flow cytometry and trilineage differentiation. …”
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KRT23 as a Potential Target for Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD): Evidence From Bioinformatics Analysis, Human Gene Polymorphism and Animal Experiments by Hao Y, Zhang T, Tuerxunmaimaiti S, Tian Y, Wang X, Li Z, Zhao L, Bai L, Chen Q, Li C, Abulitipu A, Wang R, Jiang S, Du G

“…Keratin 23 (KRT23), a liver-enriched protein linked to metabolic regulation, remains understudied in MAFLD genetics.Objective: To investigate associations between KRT23 gene polymorphisms, expression, and MAFLD in Xinjiang.Methods: This study enrolled 1,795 MAFLD patients diagnosed via ultrasonography and metabolic criteria. …”
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A Cross-Sectional Evaluation of Opioid Dispensing Competencies in Final-Year Pharm-D Students: A Multicenter Study from Punjab, Pakistan by Mubarak N, Rahman Rana F, Zahid T, Ijaz UEB, Shabbir A, Ghulam Bari A, Niaz B, Kanwal S, Saif-Ur-Rahman N, Iqbal Z, Khan AM, Zin CS, Mahmood K, Elnaem MH, Tariq S

“…Opioid dispensing competencies are critical for pharmacy graduates to promote the rational use of opioids.Purpose: To evaluate the opioids dispensing competencies among the final year Pharm-D students in Punjab, Pakistan.Design: A multicenter cross-sectional study.Methods: A validated survey was used to evaluate 11 competencies related to opioid use from the final year Pharm-D students from diversely located accredited institutes. …”
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Characteristics of positive and negative effects on the quality of life of breast cancer patients by Jin Zhao, De-Shuang Yang, Yi-Qi Liu, Yan-Kun Wu, Chao Chen, Ji-Tao Li, Ren-Gang Wu

“…However, the effects of specific emotions on patient prognosis and the relationships between them are still unclear. Methods A case–control study was conducted to investigate the characteristics of PA and NA and their effects on Quality of Life (QoL) in breast cancer patients. 442 postoperative breast cancer patients and 444 healthy women were recruited from November to December 2021. …”
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Scopoletin and Scoparone from Viola philippica Attenuate the Virulence of Pseudomonas aeruginosa by Inhibiting Quorum Sensing-Related Genes by Luo Y, Zhang S, Ou S, Shen C, Xiao Q, Chen W, Pu J, Zeng J, Zheng D, Huang B, Chen C

“…We aimed to reveal the effects of scopoletin and scoparone from V. philippica Cav. extract (VPE) on QS-related virulence phenotypes and genes in P. aeruginosa PAO1.Materials and Methods: Compounds in VPE were identified using ultrahigh-performance liquid chromatography coupled with Q-exactive hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q Exactive-Orbitrap MS). …”
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M-CSF Protects Against Ulcerative Colitis via Aconitate: Mendelian Randomization and Experimental Evidence by Zhang Y, Huang L, Yue N, Mai Z, Kong C, Tian C, Wei DR, Yao J, Wang L, Li D

“…Yuan Zhang,1,2,* Longbin Huang,3,* Ningning Yue,3,* Zhiliang Mai,3,* Chen Kong,3 Chengmei Tian,4 Dao-ru Wei,5 Jun Yao,1 Lisheng Wang,1 Defeng Li1 1Department of Gastroenterology, Shenzhen People’s Hospital (the Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China; 2Department of Medical Administration, Huizhou Institute for Occupational Health, Huizhou, Guangdong, People’s Republic of China; 3Department of Gastroenterology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University), Shenzhen, Guangdong, People’s Republic of China; 4Department of Emergency, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China; 5Department of Rehabilitation, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; the First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lisheng Wang, Department of Gastroenterology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; the First Affiliated Hospital, Southern University of Science and Technology), No. 1017, Dongmen North Road, Luohu District, Shenzhen, 518020, People’s Republic of China, Tel +86 755 25533018, Email wanglsszrmyy@163.com Defeng Li, Department of Gastroenterology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; the First Affiliated Hospital, Southern University of Science and Technology), No. 1017, Dongmen North Road, Luohu District, Shenzhen, 518020, People’s Republic of China, Tel +86 755 25533018, Email ldf830712@163.comBackground: Although cytokines have been implicated in the development of ulcerative colitis (UC), the potential mediating role of metabolite levels in this association remains unclear.Methods: Utilizing data from genome-wide association studies (GWAS) encompassing 91 circulating cytokines, 1400 blood metabolites, and 178,689 UC cases, we performed a two-sample Mendelian randomization (MR) analysis to investigate the effect of metabolites mediated cytokines on the development of UC. …”
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Association of Admission HbA1c Levels and Clinical Outcomes in Patients with Large Vessel Occlusion Following Endovascular Treatment: A Secondary Analysis of RESCUE BT Trial by Gao F, Tan X, Li L, Zhou C, Guo C, Yang J, Liu G, Wang L, Shi X, Yang S, Ma J, Xu X, Yang D, Sun W, Yu N, Fan S, Xu R, Song J, Zi W, Shang Z

“…Fei Gao,1,* Xiaolin Tan,2,* Linyu Li,1,3,* Chao Zhou,4 Changwei Guo,1 Jie Yang,1 Guojian Liu,1 Lilan Wang,1 Xiaolei Shi,1 Shihai Yang,1 Jinfu Ma,1 Xu Xu,1 Dahong Yang,1 Wenzhe Sun,1 Nizhen Yu,1 Shitao Fan,1 Rui Xu,1 Jiaxing Song,3 Wenjie Zi,1 Zhenying Shang5 1Department of Neurology, Xinqiao Hospital and The Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, People’s Republic of China; 2Department of Neurology, Meishan Second People’s Hospital, Sichuan, People’s Republic of China; 3Department of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 4Department of Neurology, Drum Tower Hospital, Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Jiangsu, People’s Republic of China; 5Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wenjie Zi, Department of Neurology, Xinqiao Hospital and The Second Affiliated Hospital, Army Medical University (Third Military Medical University), No. 183 Xinqiao Main Street, Shapingba District, Chongqing, 400037, People’s Republic of China, Email ziwenjie1981@163.com Zhenying Shang, Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Jiangsu, 221002, People’s Republic of China, Email drshang01@163.comBackground: This study aimed to analyze the association of HbA1c levels and outcomes in patients with large vessel occlusion (LVO) undergoing endovascular treatment (EVT).Methods: Patients with recorded HbA1c values were enrolled from The Endovascular Treatment With vs Without Tirofiban for Patients with Large Vessel Occlusion Stroke (RESCUE BT) trial. …”
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Genomic and Transcriptomic Profiles in Smokers and Never-Smokers Lung Squamous Cell Carcinoma Patients by Canale M, Virga A, Angeli D, Fonzi E, Gnetti L, Dubini A, Tedaldi G, Urbini M, Bocchialini G, Petracci E, Verlicchi A, Cravero P, Citarella F, Bennati C, Andrikou K, Delmonte A, Crinò L, Carbognani P, Ulivi P, Ampollini L

“…Molecular profiling of SCC in never-smokers could unveil tumor vulnerabilities and new treatment strategies.Patients and Methods: We considered a patient cohort of 17 former or current smokers (51.5%) and 16 never-smoker SCC patients (48.5%). …”
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Development and external validation of a nomogram prediction model based on quantitative coronary angiography for predicting ischemic lesions: a multi-centre study by Shuai Yang, Shuai Yang, Shuang Leng, Shuang Leng, Zhouchi Wang, Jiang Ming Fam, Jiang Ming Fam, Adrian Fatt Hoe Low, Adrian Fatt Hoe Low, Ru-San Tan, Ru-San Tan, Ping Chai, Ping Chai, Lynette Teo, Lynette Teo, Chee Yang Chin, Chee Yang Chin, John C. Allen, Mark Yan-Yee Chan, Mark Yan-Yee Chan, Khung Keong Yeo, Khung Keong Yeo, Aaron Sung Lung Wong, Aaron Sung Lung Wong, Soo Teik Lim, Soo Teik Lim, Qinghua Wu, Liang Zhong, Liang Zhong, Liang Zhong

“…This study aimed to construct and validate a QCA-based prediction model, represented as a nomogram, for predicting ischemic lesions defined by invasive fractional flow reserve (FFR) ≤ 0.80.MethodsIn this multi-centre study, we enrolled 220 patients with 303 interrogated vessels who underwent FFR measurements during clinically indicated invasive coronary angiography. …”
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Adolescents and adults with FOXP1 syndrome show high rates of anxiety and externalizing behaviors but not psychiatric decompensation or skill loss by Tess Levy, Tess Levy, Hailey Silver, Hailey Silver, Renee Soufer, Renee Soufer, Audrey Rouhandeh, Audrey Rouhandeh, Audrey Rouhandeh, Alexander Kolevzon, Alexander Kolevzon, Alexander Kolevzon, Alexander Kolevzon, Joseph D. Buxbaum, Joseph D. Buxbaum, Joseph D. Buxbaum, Joseph D. Buxbaum, Joseph D. Buxbaum, Joseph D. Buxbaum, Paige M. Siper, Paige M. Siper, Paige M. Siper

“…To date, much of the literature focuses on childhood symptoms and little is known about the FOXP1 syndrome phenotype in adolescence or adulthood.MethodsA series of caregiver interviews and standardized questionnaires assessed psychiatric and behavioral features of 20 adolescents and adults with FOXP1 syndrome. …”
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Three-Dimensional-Printed Isoniazid Chewable Gels for On-Demand Latent Tuberculosis Treatment in Children by Amanda de O. E. Moreira, Lêda Maria S. Azevedo Neta, Márcia Pietroluongo, Ana Paula dos S. Matos, Beatriz B. Correa, Beatriz H. Ortiz, André da S. Guimarães, Marcio Nele, Carollyne M. Santos, Ana Elizabeth C. Fai, Maria Helena Gonçalves, Flávio M. Shimizu, Monique S. Dos Santos, Rosemberg B. Moure, Diogo D. Nascimento, André Luis de A. Guimarães, Saint Clair dos S. G. Junior, Alessandra L. Vicosa, Lucio M. Cabral

“…This study aims to develop innovative 3D-printed chewable gels containing INH for LTBI treatment. <b>Methods</b>: The gels were formulated using gelatin and carrageenan gum, sugar-free sweeteners, and flavoring. …”
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Multimodal abnormalities of brain function in chronic low back pain: a systematic review and meta-analysis of neuroimaging studies by Xingyao Chen, Xingyao Chen, Xingyao Chen, Nuo Chen, Nuo Chen, Nuo Chen, Peng Lai, Peng Lai, Yiqi Sun, Jie Yu, Ming Xin, Deliang Zhu, Fanrong Liang, Fanrong Liang, Fanrong Liang, Qian Song, Shirui Cheng, Shirui Cheng, Shirui Cheng, Zhengjie Li, Zhengjie Li, Zhengjie Li, Zhengjie Li

“…In this meta-analysis, we integrated the existing data, delineating a pattern of coherent results from the encompassed studies.MethodsA systematic search of neuroimaging studies investigating the brain activity differences between CLBP and Healthy controls (HCs) was conducted in seven databases up to December 22, 2024. …”
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Greater neural pattern similarity to the native language is associated with better novel word learning by Yuan Feng, Yuan Feng, Yuan Feng, Yuan Feng, Aqian Li, Aqian Li, Aqian Li, Aqian Li, Jing Qu, Jing Qu, Jing Qu, Jing Qu, Huiling Li, Huiling Li, Huiling Li, Huiling Li, Xiaoyu Liu, Xiaoyu Liu, Xiaoyu Liu, Xiaoyu Liu, Jingxian Zhang, Jingxian Zhang, Jingxian Zhang, Jingxian Zhang, Jiayi Yang, Jiayi Yang, Jiayi Yang, Jiayi Yang, Leilei Mei, Leilei Mei, Leilei Mei, Leilei Mei

“…Nevertheless, it is still unclear how the utilization of the L1 neural strategies affects visual word learning in a new language.MethodsTo address this question, the present study scanned native Chinese speakers while performing implicit reading tasks before 9-day form-meaning learning in Experiment 1 and before 12-day comprehensive word learning in Experiment 2. …”
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Squalomix: shark and ray genome analysis consortium and its data sharing platform [version 2; peer review: 2 approved] by John Rozewicki, Kazuaki Yamaguchi, Osamu Nishimura, Ryo Misawa, Takashi Asahida, Mitsumasa Koyanagi, Akihisa Terakita, Filipe Castro, André Machado, Milton Tan, Salvatore D'Aniello, Shotaro Hirase, Akane Kawaguchi, Chiharu Tanegashima, Akinori Teramura, Masaru Yagura, Taiki Niwa, Kaori Tatsumi, Yuta Ohishi, Susumu Hyodo, Mitsutaka Kadota, Itsuki Kiyatake, Keiichi Sato, Kazuyuki Yamada, Kiyonori Nishida, Yasuko Semba, Shinya Yamauchi, Sho Tanaka, Tatsuya Sakamoto, Norio Miyamoto, Atsushi Toyoda, Rui Matsumoto, Iker Irisarri, Atsuko Yamaguchi, Tazro Ohta, Kiyomi Murakumo, Junna Kawasaki, Masayuki Horie, Yoshinobu Uno, Shigehiro Kuraku

“…Their peculiar body fluid composition has also hindered the establishment of methods to perform routine cell culturing required for their karyotyping. …”
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Distinct immunity dynamics of natural killer cells in mild and moderate COVID-19 cases during the Omicron variant phase by Yukari Nishikawa, Kosuke Yamaguchi, Ma’arif Athok Shofiudin, Momone Mimura, Miyako Takata, Shu Mihara, Takeru Kawakami, Ayumu Doi, Risa Matsuda, Hiroyuki Kato, Ryo Okamoto, Kengo Mukuda, Naoki Kinoshita, Kensaku Okada, Tsuyoshi Kitaura, Masaki Nakamoto, Hisashi Noma, Yusuke Endo, Akira Yamasaki, Hiroki Chikumi

“…This study analyzed alterations in natural killer (NK) cell-associated immunity dynamics in mild and moderate COVID-19 cases during the Omicron phase of the COVID-19 pandemic.MethodsWe conducted a retrospective observational cohort study of patients aged ≥16 with confirmed SARS-CoV-2 infection who were hospitalized at Tottori University Hospital between January 2022 and May 2022. …”
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Patient-reported outcome tools of acupuncture clinical trials in mainland China: a cross-sectional study by Feng Cao, Feng Cao, Yue Dong, Zhi-Qiang Li, Zhi-Qiang Li, Xue-Feng Wang, Xue-Feng Wang, Cheng-Yuan Su, Cheng-Yuan Su, Jia-Xuan Lv, Zhong-Yu Shi, Ming-Hong Du, Xin-Yue Zhang, Hong-Guo Rong, Hong-Guo Rong, Hong-Guo Rong, Yu-Tong Fei, Yu-Tong Fei, Yu-Tong Fei

“…This study aims to evaluate the characteristics and application of PRO measurements in acupuncture clinical trials in mainland China, further exploring and developing patient-reported outcomes (PROs) that are in line with the characteristics of acupuncture treatment.MethodsThis cross-sectional study analyzed acupuncture clinical trials in mainland China (2010–2022). …”
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Elevated septal native T1 time in cardiac magnetic resonance imaging suggesting myocardial fibrosis in young kidney transplant recipients by Tim Alexander Ubenauf, Jeannine von der Born, Rizky I. Sugianto, Carl Grabitz, Elena Lehmann, Nima Memaran, Nele Kanzelmeyer, Jan Falk, Nigar Babazade, Samir Sarikouch, Diane Miriam Renz, Bernhard Magnus Wilhelm Schmidt, Anette Melk

“…This study aimed to explore cardiac function and structure with focus on myocardial fibrosis and associated risk factors in KTx recipients. Methods: Forty-six KTx recipients (mean age 16.0 ± 3.5 years) and 46 age- and sex-matched healthy controls were examined with non-contrast CMR imaging. …”
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Engineering and Evaluation of a Live-Attenuated Vaccine Candidate with Enhanced Type 1 Fimbriae Expression to Optimize Protection Against <i>Salmonella</i> Typhimurium by Patricia García, Arianna Rodríguez-Coello, Andrea García-Pose, María Del Carmen Fernández-López, Andrea Muras, Miriam Moscoso, Alejandro Beceiro, Germán Bou

“…This study aimed to improve the protective immunity of a previously developed growth-deficient strain—a double auxotroph for D-glutamate and D-alanine—by engineering the inducible expression of type 1 fimbriae. <b>Methods</b>: P<i>_tetA</i>-driven expression of the <i>fim</i> operon was achieved by λ-Red mutagenesis. …”
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Fatal risk factors and the efficacy of glucocorticoid therapy in severe fever with thrombocytopenia syndrome: a multicenter retrospective cohort study by Fang Zhong, Shiyu Zhang, Chengxi Zheng, Dilihumaer Zhayier, Shuting Liu, Qinyu You, Hongming Huang, Bin Zhu, Jin Tian, Zhiliang Hu, Zhiliang Hu, Zhiliang Hu, Xin Zheng, Baoju Wang, Zhihang Peng, Zhihang Peng, Zhihang Peng

“…Glucocorticoids (GCs) can be used as anti-inflammatory agents for SFTS, but no standardized protocols have been proposed.MethodsA total of 901 patients with SFTS diagnosed at two hospitals between July 2017 and October 2023 were included in this retrospective cohort study. …”
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