Targeted Isolation of ω-3 Polyunsaturated Fatty Acids from the Marine Dinoflagellate <i>Prorocentrum lima</i> Using DeepSAT and LC-MS/MS and Their High Activity in Promoting Microg... by Chang-Rong Lai, Meng-Xing Jiang, Dan-Mei Tian, Wei Lu, Bin Wu, Jin-Shan Tang, Yi Zou, Song-Hui Lv, Xin-Sheng Yao

“…Through this approach, four new (<b>1</b>–<b>4</b>) and nine known (<b>5</b>–<b>13</b>) PUFA analogues were obtained from large-scale cultures of the marine dinoflagellate <i>Prorocentrum lima</i>, with lipidomic profiling identifying FA18:5 (<b>5</b>), FA18:4 (<b>7</b>), FA22:6 (<b>8</b>), and FA22:6 methyl ester (<b>11</b>) as major constituents of the algal oil extract. Structural elucidation was achieved through integrated spectroscopic analyses of IR, 1D and 2D NMR, and HR-ESI-MS data. …”
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Implementation of a Patient Navigation Program to Support Representative Participation in Cancer Clinical Trials by Sukh Makhnoon, Fabian Robles, Jessica L. Lee, D'Angelo Grant, Marisol Rojas, Ang Gao, Carla Pezzia, Venkata Eluri, Navid Sadeghi, Song Zhang, Thomas Hulsey, Rebecca Renn, Erin L. Williams, Heather Kitzman, David E. Gerber

“…Evaluation was guided by the Reach, Effectiveness, Adoption, Implementation, and Maintenance framework and incorporated programmatic data, structured surveys of patients and staff, and qualitative patient interviews. …”
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E2F1-induced autocrine IL-6 inflammatory loop mediates cancer-immune crosstalk that predicts T cell phenotype switching and therapeutic responsiveness by Alf Spitschak, Prabir Dhar, Krishna P. Singh, Rosaely Casalegno Garduño, Shailendra K. Gupta, Shailendra K. Gupta, Julio Vera, Luca Musella, Nico Murr, Anja Stoll, Brigitte M. Pützer, Brigitte M. Pützer

“…To explore E2F1’s role in immune regulation in presence of aggressive melanoma cells, we established a coculture system and utilized transcriptome and cytokine arrays combined with bioinformatics and structural modeling. We identified an E2F1-dependent gene regulatory network with IL6 as a central hub. …”
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Australian treatment outcome study: protocol for the 18–20-year follow-up of a prospective longitudinal cohort examining the natural history of heroin dependence and associated mor... by Maree Teesson, Paul S Haber, Christina Marel, Katherine Mills, Rachel Visontay, Jack Wilson, Shane Darke, Joanne Ross, Tim Slade, Katherine Haasnoot, Madeleine Keaveny, Chris Tremonti

“…The 18–20-year follow-up will consist of: (1) a structured interview; (2) physical health assessment; and (3) data linkage. …”
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Prevalence of tick-borne pathogens in feeding and questing Ixodes ricinus ticks from Southern Sweden by Chiara Cialini, Alessandra Cafiso, Mattias Waldeck, Åsa Lundgren, Johan Fält, Bo Settergren, Phimphanit Choklikitumnuey, Giulia Chiappa, Eleonora Rosso, Laura Roveri, Elisa Fesce, Nicola Ferrari, Per-Eric Lindgren, Chiara Bazzocchi, Giulio Grandi

“…This study highlights the presence of several zoonotic TBPs in Skåne, emphasizing the need for a structured monitoring plan and preventive strategies within a One Health framework.…”
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Patient participation in free cataract surgery: a cross-sectional study of the low-income elderly in urban China by Bo Qu, Hui Chen, Xuhua Tan, Xiaohang Wu, Haotian Lin, Zhenzhen Liu, Lixia Luo, Bo Zhang, Erping Long, Duoru Lin, Weirong Chen, Yizhi Liu, Haofeng Jiang, Jingjing Chen, Zhuoling Lin, Xiaoyan Li, Jinzhu Tang, Yingfen Lin

“…In this study, participants who underwent free cataract surgery between January and August 2014 received a telephone interview based on a structured questionnaire. Data were collected on patient demographics, resources, health conditions, reasons for undergoing the free surgery and overall evaluation of the free cataract surgery programme.Results Among the 833 participants, the mean surgical age was 76.85±7.46 years (95% CI 76.34 to 77.36), and the male to female ratio was 385:448. …”
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Clinical genome sequencing in patients with hereditary breast and ovarian cancer: Concept, implementation and benefits by Dennis Witt, Marc Sturm, Antje Stäbler, Benita Menden, Lisa Ruisinger, Kristin Bosse, Ines Gruber, Andreas Hartkopf, Silja Gauß, German Demidov, Nicolas Casadei, Elena Buena Atienza, Kira Mehnert, Janna Witt, Caspar Gross, Leon Schütz, Christopher Schroeder, Stephan Ossowski, Andreas Dufke, Tobias B. Haack, Olaf Riess, Ulrike Faust

“…GS offers a wealth of information, including the improved detection of structural variants, copy number variants, and parallel detection of complex genetic markers. …”
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The antioxidant property of CAPE depends on TRPV1 channel activation in microvascular endothelial cells by Miltha Hidalgo, Bárbara Railef, Vania Rodríguez, Carolina Navarro, Vanessa Rubio, Jorge Meneses-Pacheco, Sandra Soto-Alarcón, Christine Kreindl, Carolina Añazco, Leandro Zuñiga, Omar Porras

“…Herein, we hypothesized that a subfamily of TRP ion channels works as the molecular entity that recognizes CAPE at the plasma membrane and allows a fast shift in the antioxidant capacity of intact endothelial cells (EC).By monitoring cytoplasmic Ca2+ in a microvascular EC model, we compared the calcium responses evoked by three structurally related compounds: caffeic acid phenethyl ester, neochlorogenic acid and caffeic acid. …”
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MERS-CoV seroconversion amongst Malaysian Hajj pilgrims returning from the Middle East, 2016–2018: results from the MERCURIAL multiyear prospective cohort study by Jefree Johari, Robert D. Hontz, Brian L. Pike, Tupur Husain, Norhayati Rusli, Rozainanee Mohd-Zain, Vunjia Tiong, Hai-Yen Lee, Boon-Teong Teoh, Sing-Sin Sam, Chee-Sieng Khor, Shih-Keng Loong, Juraina Abd-Jamil, Siti-Sarah Nor'e, Hasmawati Yahaya, Naim Che-Kamaruddin, Jose A. Garcia-Rivera, Sazaly AbuBakar

“…ELISAs and micro-neutralization assays were performed to detect the presence of MERS-CoV IgG antibodies. Sociodemographic data, symptoms experienced during Hajj, and history of exposure to camels or camel products were recorded using structured pre- and post-Hajj questionnaires. …”
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Traditional uses and importance of Strychnos L. (Loganiaceae): A cross-cultural and spatial variation study in Benin by Donald Djidohokpin, Gbèwonmèdéa Hospice Dassou, Gnimansou Abraham Favi, Octave Mathieu Zountangni, Jéronime Marie-Ange Sènami Ouachinou, Judicael Makponsè, Ghyslain Kpetikou, Gbèkponhami Monique Tossou, Hounnankpon Yédomonhan, Aristide Cossi Adomou

“…Detailed information about uses and homemade herbal remedies regarding the medicinal category was collected. Data were analyzed through the citation frequencies and homemade remedy report (HR) using Excel. …”
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Optical genome mapping: Unraveling complex variations and enabling precise diagnosis in dystrophinopathy by Jiahui Mai, Jing Duan, Xiaoyu Chen, Liqin Liu, Dachao Liang, Tao Fu, Gang Lu, Wai Yee Chan, Xufeng Luo, Feiqiu Wen, Jianxiang Liao, Zhuo Li, Xinguo Lu

“…We used the optical genome mapping (OGM) technology to detect and analyze uncommon mutations or structural variations (SVs) within the DMD gene, thus contributing to more precise clinical diagnoses. …”
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Audio and linguistic prediction of objective and subjective cognition in older adults: what is the role of different prompts? by Varsha D. Badal, Varsha D. Badal, Caitlyn Tran, Haze Brown, Danielle K. Glorioso, Danielle K. Glorioso, Rebecca Daly, Rebecca Daly, Anthony J. A. Molina, Anthony J. A. Molina, Alison A. Moore, Alison A. Moore, Erhan Bilal, Ellen E. Lee, Ellen E. Lee, Ellen E. Lee, Colin A. Depp, Colin A. Depp, Colin A. Depp

“…BackgroundPsycho-linguistic and audio data derived from speech may be useful in screening and monitoring cognitive aging. …”
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Assessment of usage, reuse and disposal of thermoplastic masks among radiotherapy technologists in India: A nationwide perspective by M. Nagesh, Rohit Vadgaonkar, K.K. Sreelakshmi, Raghavendra Hajare, Pritam Parab, Subhabrat Dash, Ramesh Reddy, Vaishali Shende, Ankita Nawar, Rahul Biswas, Sandeep Ratna Bula, Shubham Gagare, Darshani Belekar, Raviteja Miriyala, Umesh Mahantshetty

“…Materials and Methods: A cross-sectional survey was conducted among RTTs working in different healthcare settings. A structured questionnaire designed by a team of RTTs and radiation oncologists was used to collect responses. …”
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CTV-MIND: A cortical thickness-volume integrated individualized morphological network model to explore disease progression in temporal lobe epilepsy by Xinyan Liu, Jiaqi Han, Xiating Zhang, Boxuan Wei, Lu Xu, Qilin Zhou, Yuping Wang, Yicong Lin, Jicong Zhang

“…This study aimed to investigate progressive changes in TLE by constructing a novel individualized morphological brain network based on T1-weighted structural magnetic resonance imaging (MRI). MRI data were collected from 34 postoperative seizure-free TLE patients and 28 age- and sex-matched healthy controls (HC), with patients divided into LONG-TERM and SHORT-TERM groups. …”
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Prevalence and risk factors of Helicobacter pylori infection in urban children aged 6–13 years in Fuzhou, China: a cross-sectional study in a high gastric cancer incidence region by Youtao Chen, Youtao Chen, Youtao Chen, Xiaoting Pan, Xiaoting Pan, Xiaoting Pan, Yanyan Wang, Yanyan Wang, Longzhen Lin, Xiaoyan Chen, Xiaoyan Chen, Yan Chen, Yan Chen, Hong Ye, Hong Ye, Hong Ye

“…BackgroundThe burden of Helicobacter pylori infection remains a global public health concern, particularly due to its lifelong risk of gastric cancer starting from childhood. Comprehensive data on the prevalence of H. pylori infection and associated risk factors among children are limited. …”
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Safety of mycophenolate mofetil in systemic lupus erythematosus maintenance therapy: insights from the LUNA registry in a nationwide prospective cohort study by Ken-ei Sada, Shuhei Yoshida, Kenji Saito, Yuya Fujita, Eisuke Inoue, Kiyoshi Migita, Naoki Matsuoka, Hiroshi Kajiyama, Ryusuke Yoshimi, Kunihiro Ichinose, Yoshia Miyawaki, Shigeru Ohno, Nobuyuki Yajima, Yasuhiro Shimojima, Shuzo Sato, Michio Fujiwara, Keisuke Nishimura, Takashi Kida, Yuya Sumichika, Haruki Matsumoto, Jumpei Temmoku, Tomoyuki Asano, Shotaro Ogawa, Jumpei Fukita, Yusuke Matsuo, Takahisa Onishi

“…Statistical analyses employed marginal structural models with stabilised inverse probability of treatment weighting to adjust for confounders.Results The analysis included 1004 patients; the incidence of severe infections was 6.5% in the MMF group and 7.5% in the non-MMF group, with no significant difference (OR 0.69, 95% CI 0.34 to 1.39). …”
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Bringing a Systems Approach to Living Donor Kidney Transplantation by Anna Horton, Katya Loban, Peter Nugus, Marie-Chantal Fortin, Lakshman Gunaratnam, Greg Knoll, Istvan Mucsi, Prosanto Chaudhury, David Landsberg, Michel R. Pâquet, Marcelo Cantarovich, Shaifali Sandal

“…Findings may be used to guide structured organizational change toward increasing LDKT and mitigating the global burden of kidney failure.…”
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The mediating role of physical activity avoidance in the association between weight stigma and physical activity by Po-Ching Huang, Hung-Ching Wu, Ji-Kang Chen, Xavier C.C. Fung, Nadia Bevan, Daniel Kwasi Ahorsu, Mark D. Griffiths, Jung-Sheng Chen, Kuo-Hsin Lee, Amir H. Pakpour, Chung-Ying Lin

“…Results: Structural equation modeling showed that weight-related self-stigma was significantly associated with PA avoidance (standardized coefficient [β] = 0.67, p < 0.01), and negatively associated with PA (β = −0.14, p < 0.01). …”
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Executive dysfunction relates to salience network desegregation in behavioural variant frontotemporal dementia by Melanie A. Matyi, Hamsanandini Radhakrishnan, Christopher A. Olm, Jeffrey S. Phillips, Philip A. Cook, Emma Rhodes, James C. Gee, David J. Irwin, Corey T. McMillan, Lauren Massimo

“…Given that behavioral variant frontotemporal degeneration (bvFTD) is a neurodegenerative disease associated with executive dysfunction and selective vulnerability of the salience network, we tested the hypotheses that bvFTD structural networks are de-differentiated compared to cognitively normal controls (CNC) and that network de-differentiation relates to worse executive function. …”
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Single-cell chromatin accessibility profiling reveals regulatory mechanisms and evolution in pig brains by Yue Xiang, Saixian Zhang, Yi Huang, Zhuqing Zheng, Jiahui Sun, Qiulin Zhao, Peng Zhou, Xiaolong Qi, Jingjin Li, Fuyang Xiong, Jing Xu, Shengquan Wang, Liangliang Fu, Xinyun Li

“…Abstract Background Pig brains serve as a valuable biomedical model for studying brain-related diseases due to their significant structural similarities to the human brain. Furthermore, the long-term domestication and artificial selection of domestic pigs have profoundly shaped their brains, making them an interesting subject for research. …”
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