Technical note: Flow cytometry assays for the detection, counting and cell sorting of polyphosphate-accumulating bacteria by C. Bouquet, H. Billard, H. Billard, C. C. Bidaud, J. Colombet, J. Colombet, Y.-T. Chang, J. Artigas, I. Batisson, K. Benzerara, F. Skouri-Panet, E. Duprat, A.-C. Lehours

“…This requires high-throughput methods to characterize their structure and dynamics in ecosystems. A promising strategy is to combine the staining of intracellular polyphosphate granules and their subsequent detection by flow cytometry, enabling rapid data acquisition. …”
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Erythritol as a Next-Generation Sweetener: properties, metabolism, cardiovascular risk and gut health implications – A literature review by Julia Skowrońska-Borsuk, Martyna Narożniak, Adrianna Ewa Pękacka, Bartłomiej Czerwiec, Adam Borsuk, Joanna Katarzyna Pergoł, Malwina Wojtas, Julia Borkowska, Zuzanna Krupa, Julia Sposób

“…However, these findings are inconclusive and require further investigation. Emerging animal data indicate erythritol may modulate gut epithelial structure via microbiota-derived metabolites. …”
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Anti-Migratory Activity of Brazilin Chemodiversification on Breast Cancer Cells by Alberto Hernández-Moreno, Dania A. Nava-Tapia, Miriam D. Zuñiga-Eulogio, Jorge Bello-Martínez, Monserrat Olea-Flores, Tadeo Hernández-Moreno, Mario Ordoñez, Ana E. Zacapala-Gómez, Miguel A. Mendoza-Catalán, Napoleón Navarro-Tito

“…In this study, we isolated brazilin from the heartwood of <i>Haematoxylum brasiletto</i>; then, we performed a semi-synthesis by adding three methyl or acetyl groups to the core structure of brazilin. We confirmed the identity of brazilin and its derivatives by spectroscopic data (¹H NMR and ¹³C NMR) and measured their purity by optical rotation. …”
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An effective fluorescence strategy based on xanthine oxidase catalysis and the carbon dots’ specific response to ·OH for aminophylline pharmacokinetic studies by Chenfang Miao, Menghan Zhang, Ying Zheng, Xiaoyan Lin, Shuangying Yang, Linlin Xu, Sitong Lu, Zhengjun Huang, Jianyong Huang, Yanjie Zheng, Shaohuang Weng

“…In this study, high quantum yield carbon dots (CA/EDA-CDs) were synthesized via a hydrothermal method using riboflavin as the carbon source, citric acid and ethylenediamine as functionalizing agents and dopants. The luminescent structure of CA/EDA-CDs, featuring a π-conjugated system (aromatic C=C and graphite N) and surface chromophores (C=N/C=O) connected via C-N, C-O, and hydrogen bonds, was controllably modulated by hydroxyl radical (·OH). …”
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ASSESSMENT OF THE PSYCHOLOGICAL STATUS OF PATIENTS WITH GYNECOLOGICAL CANCER IN THE PROCESS OF PSYCHOLOGICAL REHABILITATION by E. V. Pestereva, V. A. Chulkova, T. Y. Semiglazova, E. G. Ulrich, O. A. Pestereva

“…Disturbing component in patients at the stage of «before operation» and dysphoric component in patients at the stage of «after surgery» were dominated on the background of ergopathic reactions to the disease in the structure of attitude to the disease. The high intensity of complaints of female patients about their health, including non-specific, indicating the psychosomatic component of the patient’s suffering was revealed. …”
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Characterization of Novel WFS1 Variants in Three Diabetes Pedigrees by ChangQing Liu, HangYu Fang, Dong Wang, YiPing Cheng, Ping Shi, ChunXiao Yu, XiaoHong Li, Hui Zhao, Wei Hou, ZhenKui Guo, Chao Xu, QingBo Guan

“…A three‐dimensional wolframin protein structure was constructed to investigate the potential effects of the mutations. …”
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Efficacy and safety of cannabidiol in a single-center pediatric drug-resistant epilepsy cohort: a retrospective study by Ambra Butera, Ambra Butera, Giulia Spoto, Graziana Ceraolo, Maria Grella, Ivana Giunta, Maria Ludovica Albertini, Carla Consoli, Caterina Sferro, Maria Spanò, Gabriella Di Rosa, Antonio Gennaro Nicotera

“…Interestingly, brain magnetic resonance imaging revealed structural abnormalities in 5/15 patients, with 6/15 showing malformations of cortical development (4/6 responders, including 1 seizure-free).ConclusionCBD demonstrated a good safety and tolerability profile and appeared to be a promising therapeutic option for the management of DRE. …”
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Phenotypically distinguishable eosinophilic cells do not impact epithelial functions in a triple-culture in vitro intestinal model by Christoph Benkstein, Laurin Mosig, Daniel Vondran, Heidi Schlichting, Lea Kissing, Bente Wohlert, Ida Gensmer, Larissa Nogueira de Almeida, Peter König, Peter König, Kerstin Fibelkorn, Anna Kordowski, Stefanie Derer-Petersen, Christian Sina, Christian Sina, Yves Laumonnier, Yves Laumonnier

“…In-depth characterization by real-time PCR, flow cytometry, functional assay and electron microscopy showed that only butyrate and dibutyryl-cAMP generated phenotypically distinct eosinophilic cells with different activation statuses, marked by differential expression of surface markers CD11c and CD62L, increased expression of eosinophil specific genes, and development of eosinophilic structural features. Then, a triple-culture system encompassing the enterocytic cell line Caco-2 and the secretory cell line HT29-MTX complemented with eosinophilic differentiated cells was established. …”
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Feasibility study of single-image super-resolution scanning system based on deep learning for pathological diagnosis of oral epithelial dysplasia by Zhaochen Liu, Zhaochen Liu, Peiyan Wang, Peiyan Wang, Nian Deng, Hui Zhang, Hui Zhang, Fangjie Xin, Xiaofei Yu, Xiaofei Yu, Mujie Yuan, Mujie Yuan, Qiyue Yu, Yuhao Tang, Keke Dou, Jie Zhao, Bing He, Jing Deng, Jing Deng

“…Both the DS30R and Nikon scanners demonstrate high sensitivity and specificity for detecting structural features in OED pathological images; however, DS30R excels at identifying certain cellular features. …”
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Quality of Surface and Groundwater in the Sierra de Amula Region, Jalisco, Mexico by Oscar Raúl Mancilla-Villa, Fabiola Villafaña-Castillo, Álvaro Can-Chulim, Rubén Darío Guevara-Gutiérrez, José Luis Olguín-López, Elia Cruz-Crespo, Jonas Alan Luna-Fletes, Juan Uriel Avelar-Roblero

“…Each sample was evaluated for its pH, electrical conductivity, and ionic concentration (Ca²⁺, Mg²⁺, Na⁺, K⁺, CO₃²⁻, HCO₃⁻, CI⁻, SO₄²⁻). For data analysis, we determined the ionic concentrations and the salinity and sodicity indexes, including the electrical conductivity, pH, sodium adsorption ratio (SAR), and cationic ratio of soil structural stability (CROSS). …”
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Theoretical open-loop model of respiratory mechanics in the extremely preterm infant. by Laura Ellwein Fix, Joseph Khoury, Russell R Moores, Lauren Linkous, Matthew Brandes, Henry J Rozycki

“…However, this mode is often not successful in the extremely preterm infant in part due to their markedly increased chest wall compliance that does not provide enough structure against which the forces of inhalation can generate sufficient pressure. …”
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Creep Behavior and Long-Term Deformation Prediction of Ground Reinforced by High-Permeability Grouting by Li Chenhui, Xu Zhipeng, Zhou Pengkun, Zhang Hongbo, Zheng Yantao, Li Runguo, Liu Changwu

“…This phenomenon may lead to gradual surface settlement and structural instability, thereby compromising the long-term safety and performance of underground infrastructure. …”
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Research of indicators of activity and participation of disabled people living in inpatient social service institutions and families based on the provisions of the international cl... by I. S. Ishutina, M. D. Khodakovskii, S. V. Pavlova, A. V. Sokurov, T. V. Ermolenko

“…General scientific methods (content analysis, analytical, system-structural, comparative, statistical) and methodological methods of systematization, comparison, generalization, interpretation using the standard Excel-7.0 software package for IBM PC are applied.The object of the study. …”
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Subepicardial adipose genes contribute to the deterioration of heart failure preserved ejection fraction by Ruiying Zhang, Ruiying Zhang, Ruiying Zhang, Man Wang, Man Wang, Man Wang, Yuheng Lang, Yuheng Lang, Jiaqi Zhang, Jiaqi Zhang, Jiaqi Zhang, Yuchao Wang, Yuchao Wang, Yuchao Wang, Han Zheng, Han Zheng, Han Zheng, Yue Zheng, Yue Zheng, Yue Zheng, Yue Zheng, Bingyang Zhou, Bingyang Zhou, Bingyang Zhou

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Refractory and Volatile Species in the UV-to-IR Transmission Spectrum of Ultra-hot Jupiter WASP-178b with HST and JWST by Joshua D. Lothringer, Katherine A. Bennett, David K. Sing, Brian Kehoe-Seamons, Zafar Rustamkulov, Henrique Reggiani, Kevin C. Schlaufman, Patrick McCreery, Seti Norris, Peter Hauschildt, Ceiligh Cacho-Negrete, Amélie Gressier, Néstor Espinoza, Cyril Gapp, Thomas M. Evans-Soma, Kevin B. Stevenson, Hannah Wakeford, Neale Gibson, Jamie Wilson, Nikolay Nikolov

“…Atmospheric retrievals find a range of compositional interpretations depending on which data set is retrieved, the type of chemistry assumed, and the temperature structure parameterization used due to the combined effects of thermal dissociation, the lack of volatile spectral features besides H _2 O and CO, and the relative weakness of H _2 O and CO themselves. …”
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Emotional literacy programme in special schools for children with a learning disability in England: the ZF-SEND feasibility RCT by Biza Stenfert Kroese, Gemma Unwin, Richard Hastings, Andrew Jahoda, Rachel McNamara, David Gillespie, Jeremy Segrott, Kate Ingarfield, Myrsini Gianatsi, Elizabeth Randell, Zoe Mather, Barbara Barrett, Poushali Ganguli, John Rose, Mariam Sahle, Emily Warren, Nathan Da Cruz

“…The key aims were to assess the acceptability and feasibility of participating in the trial, data collection and the Zippy’s Friends special educational needs and disabilities intervention through quantitative and qualitative data collection. …”
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Novel Catalysts Based on Synthetic Mesoporous Silicates of the MCM-41 Type and Hydroxyapatite for Desulfurization of Model Fuel by Nadezhda O. Donskaya, Margarita A. Goldberg, Alexander S. Fomin, Anna O. Koptelova, Polina D. Domashkina, Ekaterina A. Eseva, Olga S. Antonova, Anatoliy A. Konovalov, Alexander V. Leonov, Egor A. Kudryavtsev, Fadis F. Murzakhanov, Marat R. Gafurov, Argam V. Akopyan, Sergey M. Barinov, Vladimir S. Komlev

“…The presence of aluminum and iron in the structure of the powders resulted in further amorphization. …”
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A hybrid model for refining gross primary productivity estimation by integrating multiple environmental factors by Zhilong Li, Ziti Jiao, Zheyou Tan, Chenxia Wang, Jing Guo, Sizhe Chen, Ge Gao, Fangwen Yang, Xin Dong

“…Here, we presented a hybrid model (TL-CRF) that utilizes the random forest (RF) technique to incorporate various ecological stress factors into the two-leaf LUE (TL-LUE) model, meanwhile, seasonal differences in the clumping index (CI) on a global scale are considered to adjust seasonal patterns of canopy structure. The comprehensive integration of complex environmental variables based on this RF submodule is conducive to scaling theoretical εmax to actual ε as much as possible. …”
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SMOC2 inhibits calcification of osteoprogenitor and endothelial cells. by Tine Peeters, Silvia Monteagudo, Przemko Tylzanowski, Frank P Luyten, Rik Lories, Frédéric Cailotto

“…Tissue calcification is an important physiological process required for the normal structure and function of bone. However, ectopic or excessive calcification contributes to diseases such as chondrocalcinosis, to calcium deposits in the skin or to vascular calcification. …”
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Application of the monitoring and early warning system for internal solitary waves: Take the second natural gas hydrates production test in the South China Sea as an example by Dan-yi Su, Bin-bin Guo, Qian-yong Liang, Chu-jin Liang, Fei-long Lin, Su-meng Jiang, Yi-fei Dong, Xue-min Wu

“…As suggested by the theoretical model, the full-depth structure of ISWs was a typical current profile of mode-1, and the velocities of wave-induced currents can reach 80 cm/s and 30 cm/s, respectively, in the upper ocean and near the seabed. …”
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