A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9 by Laura García-Calvo, Charlotte Kummen, Solvor Rustad, Sissel Beate Rønning, Annette Fagerlund

“…Abstract Background The yeast Komagataella phaffii (formerly known as Pichia pastoris) has been widely used for functional expression of recombinant proteins, including plant and animal food proteins. …”
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Multiple metabolic requirements for size homeostasis and initiation of division in Saccharomyces cerevisiae by Shivatheja Soma, Kailu Yang, Maria I. Morales, Michael Polymenis

“…Furthermore, we show that adenylate kinase, encoded by ADK1, is a significant determinant of the efficiency of size control mechanisms. Finally, our data argue strongly that the cell size at division is not necessarily a function of the rate cells increase in size in the G1 phase of the cell cycle. …”
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Mechanical and Civil Engineering Optimization with a Very Simple Hybrid Grey Wolf—JAYA Metaheuristic Optimizer by Chiara Furio, Luciano Lamberti, Catalin I. Pruncu

“…The proposed SHGWJA was tested very successfully in seven “real-world” engineering optimization problems taken from various fields, such as civil engineering, aeronautical engineering, mechanical engineering (included in the CEC 2020 test suite on real-world constrained optimization problems) and robotics; these problems include up to 14 optimization variables and 721 nonlinear constraints. Two representative mathematical optimization problems (i.e., Rosenbrock and Rastrigin functions) including up to 1000 variables were also solved. …”
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Simultaneous single-sample determination of NMNAT isozyme activities in mouse tissues. by Giuseppe Orsomando, Lucia Cialabrini, Adolfo Amici, Francesca Mazzola, Silverio Ruggieri, Laura Conforti, Lucie Janeckova, Michael P Coleman, Giulio Magni

“…Final assay validation was achieved in a tissue extract by comparing the activity and expression levels of individual isozymes, considering their distinctive catalytic efficiencies. Furthermore, considering the key role played by NMNAT activity in preserving axon integrity and physiological function, this assay procedure was applied to both liver and brain extracts from wild-type and Wallerian degeneration slow (Wld(S)) mouse. …”
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Dynamic multicriteria optimization of household heating and cooling system for reusing fuel-cell waste heat at optimal thermodynamic conditions while considering climatic effects by Sara Azamian

“…Energy system structures are evolving toward increasing cost benefits, efficiency, and environmental sustainability. Achieving these goals is contingent upon the utilization of renewables. …”
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Multi‐objective and multi‐stage low‐carbon planning of park integrated energy system considering random outages from superior power grid by Xunpu Jiang, Zhejing Bao, Jianwei Chen, Miao Yu

“…Then, the fuzzy membership function is used to determine the optimal compromise solution, resulting in a planning scheme that balances economic efficiency and supply reliability. …”
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Designing and implementing a Web-based real time routing service for crisis management (a case study for district 11 of Tehran) by javad sadidi, seyed hassan hosseini sajedi

“…According to the obtained results, the path’s length and traffic’s volume variables have the most important role in target function formation (travel cost) therefore the specific path will be selected as the optimal path, with the minimized distance between the destination and the traffic volume. …”
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Proposing Optimized Random Forest Models for Predicting Compressive Strength of Geopolymer Composites by Feng Bin, Shahab Hosseini, Jie Chen, Pijush Samui, Hadi Fattahi, Danial Jahed Armaghani

“…We present a comparative analysis of two hybrid models, Harris Hawks Optimization with Random Forest (HHO-RF) and Sine Cosine Algorithm with Random Forest (SCA-RF), against traditional regression methods and classical models like the Extreme Learning Machine (ELM), General Regression Neural Network (GRNN), and Radial Basis Function (RBF). Using a comprehensive dataset derived from various scientific publications, we focus on key input variables including the fine aggregate, GGBS, fly ash, sodium hydroxide (NaOH) molarity, and others. …”
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Cognitive performance classification of older patients using machine learning and electronic medical records by Monika Richter-Laskowska, Ewelina Sobotnicka, Adam Bednorz

“…Various ML techniques are evaluated to classify cognitive performance levels based on input features such as sociodemographic variables, lab results, comorbidities, Body Mass Index (BMI), and functional scales. …”
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Evaluating Sensitivity of the Ranking of Forest Fuel Treatments to Manager’s Risk Attitudes and the Importance of Treatment Objectives, Montana, USA by Tony Prato, Travis Paveglio

“…The conceptual framework involves (1) defining a utility function on an index that is a weighted average of fuel treatment objectives and incorporates a manager’s risk attitude; (2) using the utility function to calculate utility values for FTSs; (3) applying the stochastic efficiency with respect to a function method to utility values to obtain certainty equivalents (CEs); and (4) ranking FTSs based on statistically significant differences in median CEs for pairs of FTSs. …”
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Implications of thermal stratification and radiative heat flux in blood-based ternary and dihybrid nanomaterial flow through a stretchable cylinder by Muzher Saleem, Ghada A. Khouqeer, Fazal Haq, Naglaa AbdelAll, Arshad Hussain, Mohammed Sallah

“…Non-dimensional mathematical model representing the physical phenomenon is solved through NDSolve function of Mathematica. Behavior of micropolar THNF and HNF velocity and thermal field versus influential variables are investigated. …”
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Applications of mutagenesis methods on affinity maturation of antibodies in vitro by LIU Yuan, LIN Manman, ZHANG Xiao, XU Chongxin, JIAO Linxia, ZHONG Jianfeng, WU Aihua, LIU Xianjin

“…In chain shuffling, a variable heavy or light chain of a specific antibody is recombined with a complementary variable domain library. …”
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OpenConMap: A Matlab toolbox for mapping the interior of the unit circle to the exterior of simple closed curves by Kai He, Kai Wang

“…The conformal mapping function from the interior of the complex plane's unit circle to the exterior of any simple closed curve on the real plane finds widespread applications, including the use of complex variable methods in elasticity studies. …”
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Comparative Analysis of ANFIS and State-ANFIS for Forecasting Cooking Oil Prices Based on Processed Palm Oil Yield (Crude Palm Oil) by Brodjol Sutijo Suprih Ulama, Fausania Hibatullah, Mochammad Reza Habibi, null Makkulau

“…Our analysis underscores S-ANFIS’s superiority over ANFIS, particularly with Gaussian membership functions, as it reduces RMSE and MAPE values by half while requiring fewer nodes, thereby improving computational efficiency. …”
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Diversity and interdomain networks of bacterial, pico-protist and nano-protist communities in a marine ranching by Xinyi Zheng, Xin Guo, Xin Guo, Xiaoqing Lin, Cheng Huang, Lingfeng Huang

“…Although seasonal fluctuations in environmental variables generally influence microbial community dynamics, our comprehension of interdomain microbial co-occurrence patterns remains incomplete. …”
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Optimal Design Method of Integrated Energy Systems Considering Staged Planning and Equipment Replacement by Lei ZOU, Yiming TANG, Zhuping LIU, Zhengyong WU, Chao FANG, Chaoqun WANG, Fushuan WEN

“…Then, with the whole project cycle as the optimization time scale, the relationship between the equipment installation year and the current remaining life is modeled by introducing the equipment selection and operation decision variables. Finally, considering the influence of economic factors such as nominal interest rates and inflation rates, an objective function, involving investment costs, operating costs, and equipment residual value income, is constructed, with the optimal economical performance as the goal. …”
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Mass transpiration impact on effectiveness of heat transport of ternary hybrid nanofluid with velocity slip by S. Bilal, Muhammad Yasir

“…Furthermore, to raise the efficiency and to optimize the performance of the aforementioned mechanisms insertion of the composition of distinctively natured ternary nanoparticles comprising of Cu, TiO2 and Ag are induced. …”
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Neural Network and Generalized Extended State Observer Sliding Mode Control of Hydraulic Cylinder Position in the Independent Metering Control System with Digital Valves by Xiangfei Tao, Kailei Liu, Jing Yang

“…The independent metering control system is renowned for its ability to independently regulate the flow and pressure of various actuators, achieving high efficiency and energy savings in hydraulic systems. …”
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Predicting biogas production in real scale anaerobic digester under dynamic conditions with machine learning approach by M. Erdem Isenkul, Sevgi Güneş-Durak, Yasemin Poyraz Kocak, İnci Pir, Mertol Tüfekci, Güler Türkoğlu Demirkol, Selçuk Sevgen, Aslı Seyhan Çığgın, Neşe Tüfekci

“…In recent years, the use of machine learning techniques (ML) has become widespread for analysing the effects of operational factors on anaerobic digestion efficiency. Among these, Support Vector Regression (SVR) with a Radial Basis Function (RBF) kernel has been used to predict biogas yield based on diverse operating parameters. …”
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Interval Economic Dispatch for Commercial Campus Integrated Energy System with Demand Response Considering Multiple Uncertainties by Xuan Sheng, Shunjiang Lin, Weikun Liang, Yue Pan, Mingbo Liu

“…Test results from actual CCIES demonstrate that the DR can improve the economy and reduce the uncertain fluctuation range of both the objective function and state variables. The ED result can maintain an economical and secure operation under multiple uncertain fluctuations.…”
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