A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9 by Laura García-Calvo, Charlotte Kummen, Solvor Rustad, Sissel Beate Rønning, Annette Fagerlund

“…Abstract Background The yeast Komagataella phaffii (formerly known as Pichia pastoris) has been widely used for functional expression of recombinant proteins, including plant and animal food proteins. …”
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Machine learning-driven benchmarking of China's wastewater treatment plant electricity consumption by Minjian Li, Chongqiao Tang, Junhan Gu, Nianchu Li, Ahemaide Zhou, Kunlin Wu, Zhibo Zhang, Hui Huang, Hongqiang Ren

“…To overcome the complexity of wastewater compositions, an unsupervised machine learning algorithm, spectral clustering, is introduced to analyze 2,576 WWTPs across China, effectively characterizing influent quality as a single variable and contributing to robust benchmarks with 75 % of the fittings achieving coefficients of determination (R2) >0.85. …”
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Homogenization in Sobolev Spaces with Nonstandard Growth: Brief Review of Methods and Applications by Brahim Amaziane, Leonid Pankratov

“…We review recent results on the homogenization in Sobolev spaces with variable exponents. In particular, we are dealing with the Γ-convergence of variational functionals with rapidly oscillating coefficients, the homogenization of the Dirichlet and Neumann variational problems in strongly perforated domains, as well as double porosity type problems. …”
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Predictive model for the therapeutic effect of bilateral subthalamic nucleus deep brain stimulation on the freezing of gait in Parkinson’s disease by Qi Limuge, Yuqing Zhang, Xiaofei Jia, Fang Wang, Dongsheng Yu, Lixia Chen, Li Zhang, Yongqiang Jiang, Yongqiang Jiang

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A New Multi-Parametric MRI-Based Scoring System for Degenerative Cervical Myelopathy: The Severity on Imaging Myelopathy Score (SIMS) by Alexis Morgado, Julien Berthiller, Fabien Subtil, Donato Creatura, Gildas Patet, Nathalie André-Obadia, Cédric Yves Barrey

“…<b>Background/Objectives:</b> Degenerative cervical myelopathy (DCM) is the leading cause of functional disabilities of spinal origin in people over 50 years old. …”
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RAMAS-Net: a module-optimized convolutional network model for aortic valve stenosis recognition in echocardiography by Yejia Gan, Wanzhong Huang, Yan Deng, Xiaoying Xie, Yuanyuan Gu, Yaozhuang Zhou, Qian Zhang, Maosheng Zhang, Yangchun Liu

“…IntroductionAortic stenosis (AS) is a valvular heart disease that obstructs normal blood flow from the left ventricle to the aorta due to pathological changes in the valve, leading to impaired cardiac function. Echocardiography is a key diagnostic tool for AS; however, its accuracy is influenced by inter-observer variability, operator experience, and image quality, which can result in misdiagnosis. …”
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Post-thaw dimethyl sulfoxide reduction in autologous peripheral blood progenitor cell suspensions by Miroslava Jandová, Pavel Měřička, Jiří Gregor, Miriam Lánská, Aleš Bezrouk, Dana Čížková, Jakub Radocha

“…Background and objectives: Dimethyl sulfoxide has become the most common cryoprotectant used for cryopreservation of hematopoietic progenitor cells because of its efficiency, regardless of its potentially toxic side effects. …”
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The MiBlend Randomized Trial: Investigating Genetic Polymorphisms in Personalized Responses to Fruit and Vegetable Interventions for Chronic Disease Prevention by Julia N. DeBenedictis, Na Xu, Theo M. de Kok, Simone G. van Breda

“…As with most dietary intervention studies, inter-individual variability was observed in the responses, which might be the consequence of genetic differences. …”
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Computational studies reveal structural characterization and novel families of Puccinia striiformis f. sp. tritici effectors. by Raheel Asghar, Nan Wu, Noman Ali, Yulei Wang, Mahinur Akkaya

“…However, due to the lack of an efficient genetic transformation system in Pst, progress in effector function studies has been slow. …”
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Effects of shading on photosynthetic characteristics and shade-tolerance evaluation of three Ranunculaceae plants by HAN Ruiting, ZHAO Dandan, WANG Weiyi, ZHANG Gexiang*

“…The light saturation point(LSP), light compensation point(LCP)and dark respiration rate(Rd)of the three plants decreased gradually.(3)The original fluorescence(Fo)decreased first and then increased, while the maximum fluorescence(Fm), variable fluorescence(Fv), maximum photochemical efficiency of PS Ⅱ(Fv/Fm)and potential activity of PS Ⅱ(Fv/Fo)values increased first and then decreased; the quantum ratio of heat dissipation(φDo)and the energy dissipated per unit reaction center(DIo/RC)decreased first and then increased, while electron transport quantum yield(φEo), light energy absorbed per unit reaction center(ABS/RC), light energy captured per unit reaction center(TRo/RC), energy used to transfer electrons per unit reaction center(ETo/RC), photosynthetic performance index(PIabs)and comprehensive performance index(PItotal)increased first and then decreased.(4)Comprehensive analysis on 20 single indicators by using analysis methods such as principal component analysis and membership function method showed that the shade-tolerance of the three plants ranked as Thalictrum fortunei > Delphinium anthriscifolium var. savatieri > R. japonicus. …”
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REALITIES AND EFFECTIVENESS OF THE UKRAINIAN BANKING SYSTEM by Maryna Korol, Olena Bazhenova, Ihor Korol, Grygoriy Starchenko, Volodymyr Bazhenov, Oksana Banna

“…The simulation results (mainly the analysis of impulse response functions) allow concluding that interbank lending in post-crisis conditions recovers relatively rapidly (with a lag of two years). …”
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An inclusive solution based on grey water footprint for performance evaluation of industrial wastewater treatment systems by Keivan Arastou, Ali Dehnavi, Shervin Jamshidi

“…These advantages are critical for assessing an industrial WWTP treating complex wastewater, with hazardous pollutants and variable inflow. Moreover, we introduced four innovative criteria based on GWF and improved it as a multi-functional index. …”
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Strategies to support the mental health and well-being of health and care workforce: a rapid review of reviews by Cindy E. Frias, Nimesh Samarasinghe, Cecilia Cuzco, Jaseem Koorankot, Andrés de Juan, Husameldin Mohamed Ali Rudwan, Hanan F. Abdul Rahim, Adela Zabalegui, Iain Tulley, Sanaa T. Al-Harahsheh, Mona Shaheen S. T. Al-Homaiddi, Meredith Fendt-Newlin, Jim Campbell

“…The call to action for a paradigm shift must be embraced and we must strive to build resilient healthcare systems and invest in active support and sustain them, incorporating structural, non-structural and functional aspects of organizational resilience.…”
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Modelagem da produtividade e gestão da qualidade acadêmica by Annibal Parracho Sant'Anna

“…It is a hierarchical model because technical coefficients of the academic production function are modeled as function of second level explanatory variables in such a way that such production function is allowed to change coherently from one department to another. …”
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Locomotive Syndrome Digital Therapeutics Provided via a Smartphone App: Protocol for a Single-Group Trial by Tatsuru Sonobe, Yoshihiro Matsumoto

“…We evaluated the participants’ objective motor function using the timed up and go (TUG) test, and we compare the results using a 3-way ANOVA with the TUG test at the 3 evaluation time points as the dependent variable. …”
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A reduced simulation applied to viscoelastic fatigue of polymers using a time multi-scale approach based on partition of Unity method by Sebastian Rodriguez, Angelo Pasquale, Jad Mounayer, Diego Canales, Marianne Beringhier, Chady Ghnatios, Amine Ammar, Francisco Chinesta

“…In this context, the use of a space-time PGD approximation is proposed here to boost their resolution, where the temporal functions are constructed following a multi-scale strategy along with the partition of unity method, in order to catch each dynamic efficiently. …”
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Physics-augmented deep learning models for improving evapotranspiration estimation in global land regions by Binrui Liu, Xinguang He, Wenkai Lyu, Lizhi Tao

“…A Physics-Data Learning (PDL) model is first formed by adding a complementary physical variable generated by Penman–Monteith (PM) equation to a deep learning (DL) model along with driving variables to regress latent heat flux. …”
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Investigating Whether AI Will Replace Human Physicians and Understanding the Interplay of the Source of Consultation, Health-Related Stigma, and Explanations of Diagnoses on Patien... by Weiqi Guo, Yang Chen

“…The main effects and interaction effects of the variables were examined on individuals’ functional, relational, and emotional evaluations of the medical consultation. …”
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Protein expression profiling identifies a prognostic model for ovarian cancer by Luyang Xiong, Jiahong Tan, Yuchen Feng, Daoqi Wang, Xudong Liu, Yun Feng, Shusheng Li

“…The prognostic effect and the biological function of the model were evaluated using co-expression analysis and enrichment analysis. …”
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Penerapan Importance Performance Analysis (IPA) pada Evaluasi Keberlanjutan Penggunaan e-filing untuk Pelaporan Pajak Perorangan by Imelda Saluza, Dewi Sartika

“…Beberapa indikator tersebut antara lain adalah Understandability (kejelasan informasi yang diberikan oleh e-filing), Contact (kontak yang dapat dihubungi), Functionality (peningkatan kualitas dan mutu pajak), Ease of Use (e-filing sulit dipelajari), Usefulness (penghematan waktu pelaporan), Service Quality, Efficiency (pengaksesan e-filing dengan gadget lain), Fulfillment (kesulitan dalam memenuhi kebutuhan pelapor). …”
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