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    From digitized whole‐slide histology images to biomarker discovery: A protocol for handcrafted feature analysis in brain cancer pathology by Xuanjun Lu, Yawen Ying, Jing Chen, Zhiyang Chen, Yuxin Wu, Prateek Prasanna, Xin Chen, Mingli Jing, Zaiyi Liu, Cheng Lu

    Published 2025-06-01
    “…The PHBCP comprises seven main steps: (1) problem definition, (2) data quality control, (3) image preprocessing, (4) feature extraction, (5) feature filtering, (6) modeling, and (7) performance analysis. As an exemplary application, we collected pathological data of 589 patients from two cohorts and applied the PHBCP to predict the 2‐year survival of glioblastoma multiforme (GBM) patients. …”
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