Combining the RCAS/tv-a retrovirus and CRISPR/Cas9 gene editing systems to generate primary mouse models of diffuse midline glioma by Sophie R. Wu, Julianne Sharpe, Joshua Tolliver, Abigail J. Groth, Reid Chen, María E. Guerra García, Vennesa Valentine, Nerissa T. Williams, Sheeba Jacob, Zachary J. Reitman

“…Genetically engineered mouse models (GEMMs) are critical to studying tumorigenesis and tumor-immune interactions, which may inform new treatment approaches. However, current midline glioma GEMM approaches are limited in their ability to multiplex perturbations and/or target specific cell lineages in the brain for genetic manipulation. …”
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Electronic cigarette use in relation to changes in smoking status and respiratory symptoms by Linnéa Hedman<sup>+<sup>, Gustaf Lyytinen<sup>+<sup>, Helena Backman, Magnus Lundbäck, Caroline Stridsman, Anne Lindberg, Hannu Kankaanranta, Lina Rönnebjerg, Eva Rönmark, Linda Ekerljung

“…A validated postal questionnaire containing identical questions was used in OLIN and WSAS at baseline in 2006–2008 and at follow-up in 2016. …”
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The attenuated African swine fever vaccine HLJ/18-7GD provides protection against emerging prevalent genotype II variants in China by Zilong Wang, Jiwen Zhang, Fang Li, Zhenjiang Zhang, Weiye Chen, Xianfeng Zhang, Encheng Sun, Yuanmao Zhu, Renqiang Liu, Xijun He, Zhigao Bu, Dongming Zhao

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Protocol for a randomized controlled trial of patient–portal–based screening for substance use among people with HIV by Eric Roessler, Daniela Zimmer, Jon Grant, Harold Pollack, Basmattee Boodram, Jessica Schmitt, Jessica Schmitt, Eleanor Friedman, Jade Pagkas-Bather, Jade Pagkas-Bather, Russell A. Brewer, Jessica Ridgway, Neda Laiteerapong, Neda Laiteerapong

“…This will lay the groundwork for the second aim, the focus of the current manuscript, RCT to measure the effectiveness of a population health, patient portal-based substance use screening program. …”
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Intelligent beehive monitoring system based on internet of things and colony state analysis by Yiyao Zheng, Xiaoyan Cao, Shaocong Xu, Shihui Guo, Rencai Huang, Yingjiao Li, Yijie Chen, Liulin Yang, Xiaoyu Cao, Zainura Idrus, Hongting Sun

“…Moreover, our counting algorithm also achieved excellent results, with root mean square error (RMSE) of 1.3 ± 0.1, 0.2 ± 0.0, and 1.6 ± 0.1 in counting the number of bees current, entry, and out scene in an episode, respectively. …”
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An animal model study of simplified optical navigation system-assisted percutaneous lumbar transforaminal puncture by B. N. Wenxiu Chai, Yuqing Chen, Li Sheng, Yong Huang

“…The system significantly improves puncture accuracy while markedly decreasing both the number of puncture attempts and fluoroscopy exposures compared to conventional techniques.…”
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Multidisciplinary approach to the diagnosis of disorders of coagulation system in childrenwith various pathologies by O. B. Gordeeva, M. S. Karaseva, D. E. Lavretsky, M. A. Babaikina, N. D. Vashakmadze, M. A. Soloshenko, G. V. Revunenkov, E. A. Abashidze, M. V. Egorova

“…The problem of correct in time diagnostics has the socially significant nature. Currently, international consensus and national protocols for the treatment and prevention of venous thromboembolism are regularly reviewing. …”
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INTERACTION BETWEEN DENTO-MAXILLOFACIAL ANOMALIES AND GENERAL-SOMATIC HUMAN CONDITION (LITERATURE REVIEW) by L.V. Smaglyuk, H.V. Voronkova, A.Ye. Karasiunok, A.V. Liakhovska, V.I. Smaglyuk

“…Over the last decade, there has been a clear trend towards an increase in the prevalence of malocclusion, which reaches 80% according to current scientific literature and sometimes it exceeds this percentage. …”
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Synergistic Effects of Mannan Oligosaccharides and Onion Peels on In Vitro Batch Culture Fermentation of High Concentrate and Forage Diets by Lydia K. Olagunju, Oludotun O. Adelusi, Peter A. Dele, Yasmine Shaw, Rosetta M. Brice, Oluteru E. Orimaye, Jorge A. Villarreal-González, Hye Won Kang, Ahmed E. Kholif, Uchenna Y. Anele

“…The administration of MOS, OP, and MOS:2OP to the HC diet decreased methane production at 24 h of incubation. …”
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Impact of Climate Change on the Narrow Endemic Herb Psilopeganum sinense (Rutaceae) in China by Ruixiong Deng, Kaitong Xiao, Xin Chen, Beibei Huang, Haoran Li, Lin Wu, Hang Ning, Hui Chen

“…Our results not only provide a scientific basis for managing P. sinense resources in the context of climate change but also serve as an important reference for restoring wild P. sinense populations.…”
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Contribution of the TRPM4 Channel to Osteogenic Differentiation of Human Aortic Valve Interstitial Cells by Margaux Aize, Arthur Boilève, Benoit D. Roussel, Laura Brard, Harlyne Mpweme Bangando, Corentin Kerevel, Alexandre Lebrun, Hind Messaoudi, Vladimir Saplacan, Alain Manrique, Romain Guinamard, Christophe Simard

“…Mineralization and osteogenic markers (bone morphogenetic protein 2, runt‐related transcription factor 2) were decreased when pro‐calcifying media contained 9‐phenanthrol or small hairpin RNA–TRPM4. …”
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Dynamics of educational activity of students under the influence of digitalisation by D. Yu. Narkhov, E. N. Narkhova, D. V. Shkurin

“…This study is based on the use of specific approaches to industry-specific sociological knowledge (youth and education): community, system, structural-functional, institutional, temporal, thesaurus, network, panoramic (pedagogical science). The current research employs the results of a mass survey conducted among students (N = 1825, 19 universities of the Sverdlovsk region) according to the authors’ method. …”
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Complementary Strategy of Maternal Immunization with RSVpreF Vaccine and Monoclonal Antibodies for the Prevention of Respiratory Syncytial Virus Among Italian Infants: A Cost-Effec... by Barbara Polistena, Fabio Midulla, Giovanni Sotgiu, Daniela d’Angela, Roberto Di Virgilio, Federico Spandonaro

“…Methods Using a model (with a cohort framework and a Markov-type process) specifically adapted to the Italian context, the study provided cost-effectiveness and cost-utility assessment of prevention strategies adding the maternal vaccination to the existing immunization opportunities with palivizumab or nirsevimab administered to high risk and unprotected infants. …”
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Increasing day three parasitaemia is observed after treatment of patients with artemether-lumefantrine and single dose of primaquine for uncomplicated Plasmodium falciparum malaria... by Bontu Abate, Bokretsion Gidey Brhane, Tsegahun Manyazewal, Hussien Mohammed, Yonas Wuletaw, Moges Kassa, Mesay Hailu, Getachew Tollera, Geremew Tasew, Ashenafi Assefa, Eyasu Makonnen

“…These findings support the ongoing use of AL with a single dose of PQ as the primary treatment option for uncomplicated P. falciparum infections in the study area. In the context of the emergence and spread of partial artemisinin-based combination therapy(ACT) resistance in Africa, regular monitoring of the efficacy of current artemisinin-based combinations is recommended for the early detection of emerging drug resistance in the study setting and beyond.…”
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Bifidobacterium animalis ssp. lactis BX-245-fermented milk alleviates tumor burden in mice with colorectal cancer by Chengcong Yang, Musu Zha, Lu Li, Jiaqi Qiao, Lai-Yu Kwok, Dandan Wang, Yongfu Chen

“…Collectively, our data suggest that administering probiotic fermented milk containing specific functional strains such as BX245 could result in a reduction in tumor burden in CRC mice. …”
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Effects of coix seed polyphenol extract on rumen fermentation, milk production, fatty acid profile, antioxidant activity, and polyphenol content in dairy goats by X.Z. Tian, Y.Q. Xu, J.X. Qin, X. Wang, S.L. Xie, R. Chen, Q. Lu, X. Chen

“…Overall, the appropriate supplemental level of CSE was 1.5 g/kg in dairy goats under the conditions of the current experiment.…”
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STAT2/SLC27A3/PINK1-Mediated Mitophagy Remodeling Lipid Metabolism Contributes to Pazopanib Resistance in Clear Cell Renal Cell Carcinoma by Dingheng Lu, Yuxiao Li, Xinyang Niu, Jiazhu Sun, Weitao Zhan, Yuchen Shi, Kai Yu, Suyuelin Huang, Xiaoyan Liu, Liping Xie, Xueyou Ma, Ben Liu

“…While tyrosine kinase inhibitors (TKIs) are currently the first-line treatments for advanced/metastatic ccRCC, patients often develop resistance after TKI therapy. …”
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Re-use of Building Products in the Netherlands by Loriane Icibaci

“…This research departs from the desire to understand the practice of reuse of building products from a systemic standpoint, to illuminate its current condition and help to foresee future perspectives. …”
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The methyl-CpG binding domain 2 regulates peptidylarginine deiminase 4 expression and promotes neutrophil extracellular trap formation via the Janus kinase 2 signaling pathway in e... by Biao Peng, Mu-Yun Yan, Yun-Rong Chen, Fei Sun, Xu-Dong Xiang, Da Liu

“…Objective The prognosis for severe asthma is poor, and the current treatment options are limited. The methyl-CpG binding domain protein 2 (MBD2) participates in neutrophil-mediated severe asthma through epigenetic regulation. …”
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Paradigms of the agricultural system in times of COVID-19 by Luis Edgar Blanco-Capia

“…Until the end of the 20th century, world agriculture underwent a transition towards a new technological paradigm, very different from that of the green revolution, based on the current “bio” revolutions, in which human beings have transformed biodiversity by placing it at their service1. …”
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