Engineered extracellular vesicles for delivering functional Cas9/gRNA to eliminate hepatitis B virus cccDNA and integration by Wanjia Zeng, Liwei Zheng, Yukun Li, Jing Yang, Tianhao Mao, Jing Zhang, Yanna Liu, Jing Ning, Ting Zhang, Hongxin Huang, Xiangmei Chen, Fengmin Lu

“…Our results demonstrated that engineered EVs containing Cas9/gRNA and VSV-G can effectively reduce viral antigens and cccDNA levels in the HBV-replicating and infected cell models. …”
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Cytomegalovirus-Specific T Cells from Third-Party Donors Successfully Treated Refractory Cytomegalovirus Retinitis after Unrelated Umbilical Cord Blood Transplantation by Na Li, Guangyu Sun, Lihua Zhu, Kang Ding, Huilan Liu, Xiaoyu Zhu, Baolin Tang, Wen Yao, Xiang Wan, Liangquan Geng, Ping Qiang, Kaidi Song, Changcheng Zheng, Zimin Sun, Juan Tong

“…Umbilical cord blood (UCB) transplants (UCBTs) are becoming increasingly common in the treatment of a variety of hematologic and nonhematologic conditions. …”
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Evolution of taxis responses in virtual bacteria: non-adaptive dynamics. by Richard A Goldstein, Orkun S Soyer

“…We currently lack a full understanding of the different pathway structures and dynamics and how this integration is achieved. …”
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Safety of a change of specifications of phytosterols/phytostanols as a novel food pursuant to Regulation (EU) 2015/2283 by EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA), Dominique Turck, Torsten Bohn, Montaña Cámara, Jacqueline Castenmiller, Stefaan De Henauw, Karen Ildico Hirsch‐Ernst, Ángeles Jos, Alexandre Maciuk, Inge Mangelsdorf, Breige McNulty, Androniki Naska, Kristina Pentieva, Alfonso Siani, Frank Thies, Margarita Aguilera‐Gómez, Francesco Cubadda, Thomas Frenzel, Marina Heinonen, Monika Neuhäuser‐Berthold, Helle Katrine Knutsen, Morten Poulsen, Miguel Prieto Maradona, Josef Rudolf Schlatter, Alexandros Siskos, Henk vanLoveren, Wolfgang Gelbmann, Harry J. McArdle

“…Regarding the maximum limit of the specification parameter ‘other sterols/stanols’, the applicant asks for an increase from currently less than 3% to less than 7% to better reflect and cover the natural composition and proportions of sunflower‐based phytosterols/phytostanols, noting that the existing specifications have been established largely based on the NF when sourced from soy. …”
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Optimizing enzyme thermostability by combining multiple mutations using protein language model by Jiahao Bian, Pan Tan, Ting Nie, Liang Hong, Guang‐Yu Yang

“…It showed a 10.19°C increase in the melting temperature and an ~655‐fold increase in the half‐life at 58°C. …”
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Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro by E. A. Kalashnikova, R. N. Kirakosyan, A. V. Gushchin, K. G. Abubakarov, N. N. Sleptsov, S. K. Temirbekova, A. P. Glinushkin, O. V. Meleshina, N. Ya. Rebouh, M. M. Tareeva

“…Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assimilation of useful trace elements necessary for human life. Inulin is contained in plants such as jerusalem artichoke, chicory, as well as in sweet potatoes, the popularity of which is increasing every year. …”
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Clinical and laboratory assessment of the effect of hygiene products on the condition of periodontal tissues and hard tooth tissues in the treatment of caries by indirect method by I. A. Belenova, V. A. Mitronin, Z. A. Filippova, V. I. Khryachkov, M. S. Vasilyeva, N. A. Protsenko

“…According to statistics, the widespread use in modern dental practice of indirect restoration methods in the treatment of caries of the frontal group of teeth leads to an increase in the incidence of marginal periodontal disease. …”
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In Vitro Efficacy of PEI-Derived Lipopolymers in Silencing of Toxic Proteins in a Neuronal Model of Huntington’s Disease by Luis C. Morales, Luv Modi, Saba Abbasi Dezfouli, Amarnath Praphakar Rajendran, Remant Kc, Vaibhavi Kadam, Simonetta Sipione, Hasan Uludağ

“…<b>Methods:</b> This study aims to investigate the efficacy of siRNA-containing nano-engineered lipopolymers in selectively silencing the <i>HTT</i> expression in a neuronal model expressing a chimeric protein formed by the human mutated exon 1 of the <i>HTT</i> gene, tagged with GFP. …”
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Development of an easy non‐destructive particle isolation protocol for quality control of red blood cell concentrates by Marine Ghodsi, Anne‐Sophie Cloos, Anaïs Lotens, Marine De Bueger, Patrick Van Der Smissen, Patrick Henriet, Nicolas Cellier, Christophe E. Pierreux, Tomé Najdovski, Donatienne Tyteca

“…Nevertheless, there is currently no standardized isolation protocol. In a previous publication, we developed a reproducible ultracentrifugation‐based protocol (20,000 × g protocol) that allows to classify red blood cell concentrates into three cohorts according to their vesiculation level. …”
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Selective Separation of Scandium in Acidic Water Using Carboxyl Functionalized Covalent Phosphonitrile Polymers by Yu BAI, Ayinuer WUSHUER, Lei OUYANG, Lijin HUANG, Qin SHUAI

“…As the acidity of the solution increases, the adsorption efficiency of CPF-T for Sc(III) significantly decreases, and at pH<3, the adsorption rate is below 60%. …”
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Standardization of the Pharmaceutical Substance of the Drug LCS-1208 by E. V. Ignateva, I. V. Yartseva, Z. S. Shprakh, A. P. Bud'ko, L. V. Ektova, D. A. Kozin, V. Yu. Reshetnyak, O. V. Nesterova, E. A. Pankratova

“…Indolocarbazole derivatives are of increasing scientific interest for practical oncology. …”
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Optimizing Deep Learning Models with Improved BWO for TEC Prediction by Yi Chen, Haijun Liu, Weifeng Shan, Yuan Yao, Lili Xing, Haoran Wang, Kunpeng Zhang

“…Recently, deep learning models have become increasingly popular in TEC prediction. However, these deep learning models usually contain a large number of hyperparameters. …”
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Ecotoxicological effects of soft plastic fishing lures on the benthic amphipod Hyalella azteca by Beggel S, Kalis EJJ, Gilb KM, Pander J, Geist J

“…Especially soft plastic lures are increasingly used, potentially contributing to the pollution of waterbodies by the release of hazardous substances such as plasticizers. …”
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Anatomical theatre or full digitisation? Students’ preferences in field of anatomy teaching by Krzysztof Starszak, Radosław Karaś, Andrzej Skalski, Karolina Czarnecka-Chrebelska, Tomasz Lepich, Grzegorz Bajor

“…BACKGROUND: For many years, the teaching of anatomy has been based on traditional forms of imparting knowledge, but innovative solutions are currently being implemented on a large scale around the world. …”
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Twin Rooms – new experimental test cells for testing advanced facade elements by Boris Bielek, Daniel Szabó, Josip Klem, Kristína Kaniková, Alžbeta Danková

“…Reducing the energy intensity of buildings will increasingly resonate in individual political and professional circles. …”
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Inhibiting NLRP3 enhances cellular autophagy induced by outer membrane vesicles from Pseudomonas aeruginosa by Jing Ge, Min Cao, Yuyao Zhang, Tianqi Wu, Jiayi Liu, Jiang Pu, Hongye He, Zhibin Guo, Shaoqing Ju, Juan Yu

“…We demonstrated that the NLRP3 (NLR family, pyrin domain containing 3) inhibitor MCC950 can enhance autophagy induced by PA-OMVs. …”
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Preparation, mechanical, and thermal properties of CrNbO4: A novel dual-functional scale to protect RHEAs from oxidation and thermal attack by Shuang Zhang, Jian Zhang, Xiaohui Wang, Huimin Xiang, Cheng Fang, Wei Xie, Yanchun Zhou

“…Interestingly, the room temperature thermal conductivity of CrNbO4 is 1.09 W·m−1·K−1 and decreases to 0.45 W·m−1·K−1 at 1473 K, which is lower than that of most of the currently well-known thermal insulation materials. …”
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PANCAKE: A Python-based Numerical Color–Magnitude Diagram Analysis Package by Yun Zheng, Yujiao Yang, Yong-Kun Zhang, Zheng Zheng, Jing Wang, Lister Staveley-Smith, Chao-Wei Tsai, Di Li, Chao Liu, Jingjing Hu, Huaxi Chen, Donghui Quan, Yinghui Zheng, Hangyuan Li

“…The color–magnitude diagram (CMD) stands out as the most accurate tool currently available for inferring the star formation histories (SFHs) of nearby galaxies with stellar-resolved multiband data. …”
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Automatic Diagnosis of Traumatic Brain Injury Using Deep Learning with CT scan Images by Behrang Rezvani Kakhki, Hossein Zakeri, Sayyed Majid Sadrzadeh, Seyed Mohammad Mousavi, elnaz vafadar moradi, golnoush shahraki

“…Computed Tomography (CT) scans are currently the primary imaging modality for identifying intracranial injuries. …”
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Government responses to COVID-19 and impact on GBV services and programmes: comparative analysis of the situation in South Africa, Kenya, Uganda, and Nigeria by Neetu A. John, Paul Bukuluki, Sara E. Casey, Dhruvi B. Chauhan, Moriam O. Jagun, Nicoletta Mabhena, Mary Mwangi, Terry McGovern

“…As governments impose restrictive policies to contain infectious disease outbreaks, pre-existing gender-based inequalities are often exacerbated, increasing the risk of gender-based violence (GBV). …”
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