A mechanistic systems biology model of brain microvascular endothelial cell signaling reveals dynamic pathway-based therapeutic targets for brain ischemia by Geli Li, Yuchen Ma, Sujie Zhang, Wen Lin, Xinyi Yao, Yating Zhou, Yanyong Zhao, Qi Rao, Yuchen Qu, Yuan Gao, Lianmin Chen, Yu Zhang, Feng Han, Meiling Sun, Chen Zhao

“…To better understand the complex multifaceted roles of BMECs in the regulation of ischemic stroke pathophysiology and facilitate BMEC-based drug target discovery, we utilized a transcriptomics-informed systems biology modeling approach and constructed a mechanism-based computational multipathway model to systematically investigate BMEC function and its modulatory potential. …”
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Differences in the effects and action modes of gut commensals against dextran sulfate sodium-induced intestinal inflammation by Dingwu Qu, Zhennan Gu, Saisai Feng, Leilei Yu, Fengwei Tian, Hao Zhang, Wei Chen, Qixiao Zhai

“…The probiotic strains differed in regulating the intestinal microbiota, cytokines (IL-10, IL-1β and interferon (IFN)-γ), and tight junction function (Zonulin-1 and Occludin). …”
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Rhinovirus C replication is associated with the endoplasmic reticulum and triggers cytopathic effects in an in vitro model of human airway epithelium. by Talita B Gagliardi, Monty E Goldstein, Daniel Song, Kelsey M Gray, Jae W Jung, Maxinne A Ignacio, Kimberly M Stroka, Gregg A Duncan, Margaret A Scull

“…Finally, RV-C15 infection resulted in a temporary loss in epithelial barrier integrity and the translocation of tight junction proteins while a reduction in mucociliary clearance indicated cytopathic effects on epithelial function. …”
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A Novel Multi-Agent-Based Approach for Train Rescheduling in Large-Scale Railway Networks by Jin Liu, Lei Chen, Zhongbei Tian, Ning Zhao, Clive Roberts

“…While recent research has introduced intelligent solutions to railway traffic management, the tight interdependence of train timetables and the intrinsic complexity of railway networks have hindered the scalability of these approaches to large-scale systems. …”
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Lowe Syndrome protein OCRL1 supports maturation of polarized epithelial cells. by Adam G Grieve, Rachel D Daniels, Elena Sanchez-Heras, Matthew J Hayes, Stephen E Moss, Karl Matter, Martin Lowe, Timothy P Levine

“…We show that a proportion of OCRL1 targets intercellular junctions at the early stages of their formation, co-localizing both with adherens junctional components and with tight junctional components. Correlating with this distribution, OCRL1 forms complexes with junctional components α-catenin and zonula occludens (ZO)-1/2/3. …”
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Data-driven prediction and design of bZIP coiled-coil interactions. by Vladimir Potapov, Jenifer B Kaplan, Amy E Keating

“…Testing more than 132 candidate protein complexes using a fluorescence resonance energy transfer assay confirmed the formation of tight and selective heterodimers between the designed peptides and their targets. …”
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PREDICTION ENDOMETRIAL CANCER IN WOMEN WITH ENDOMETRIAL HYPERPLASTIC PROCESSES IN PREMENOPAUSAL by A. L. Unanyan, I. S. Sidorova, E. A. Kogan, D. V. Baburin

“…We have identified risk factors and determined their significance and relative risk in endometrial oncotransformation based on mathematical analysis of clinical and anamnestic data and molecular-biological indicators. The structural, functional and molecular mechanisms of hyperplastic processes of endometrium in premenopausal women were investigated based on a complex immunohistochemical studies. …”
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JMY powers dendritogenesis and is regulated by CaM revealing a general, critical principle in neuromorphogenesis by Maja Kühne, Anna-Lena Zepernick, Britta Qualmann, Michael Manfred Kessels, Maryam Izadi-Seitz

“…Here, we identify the WH2 domain-based actin nucleator JMY as target of the calcium sensor calmodulin, reveal that JMY is critical for dendritic arbor formation and unravel that JMY’s molecular mechanisms employed in dendritic arborization are depended on Arp2/3 complex interaction, Arp2/3 complex activity and functionality of JMY’s WH2 domains, i.e. on JMY’s abilities to promote actin filament formation. …”
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Structure and assembly of the MmpL5/MmpS5 efflux transporter from Mycobacterium tuberculosis by Zhiqi Xiong, Xiaolin Yang, Shule Wang, Caitlan J. Smart, Hazel M. Sisson, Zhenli Lin, Tianyu Hu, Yuting Ran, Chuyao Xu, Xiuna Yang, Yao Zhao, William J. Jowsey, Gregory M. Cook, Matthew B. McNeil, Luke W. Guddat, Zihe Rao, Bing Zhang

“…The stalk secures the tight association of the three MmpL5 subunits and exhibits intrinsic structural flexibility. …”
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The Drosophila hematopoietic niche assembles through collective cell migration controlled by neighbor tissues and Slit-Robo signaling by Kara A Nelson, Kari F Lenhart, Lauren Anllo, Stephen DiNardo

“…Consistency of niche position suggests that placement is important for niche function. However, the complexity of most niches has precluded a thorough understanding of how their proper placement is established. …”
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Integrative analysis identifies IL-6/JUN/MMP-9 pathway destroyed blood-brain-barrier in autism mice via machine learning and bioinformatic analysis by Cong Hu, Heli Li, Jinru Cui, Yunjie Li, Feiyan Zhang, Hao Li, Xiaoping Luo, Yan Hao

“…Abstract Autism Spectrum Disorder (ASD) is a complex neurodevelopmental condition characterized by social communication deficits and restricted, repetitive behaviors. …”
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Production, purification and characterization of recombinant, full-length human claudin-1. by Nicklas Bonander, Mohammed Jamshad, Dominik Oberthür, Michelle Clare, James Barwell, Ke Hu, Michelle J Farquhar, Zania Stamataki, Helen J Harris, Karsten Dierks, Timothy R Dafforn, Christian Betzel, Jane A McKeating, Roslyn M Bill

“…The transmembrane domain proteins of the claudin superfamily are the major structural components of cellular tight junctions. One family member, claudin-1, also associates with tetraspanin CD81 as part of a receptor complex that is essential for hepatitis C virus (HCV) infection of the liver. …”
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In silico evaluation of missense SNPs in cancer-associated Cystatin A protein and their potential to disrupt Cathepsin B interaction by Shafaat Hossain, Omar Hamza Bin Manjur, Mst Sharmin Sultana Shimu, Tamanna Sultana, Mustafizur Rahman Naim, Shahariar Siddique, Abdullah Al Mamun, Md Miftaur Rahman, Md Abu Saleh, Md Rakibul Hasan, Tania Rahman

“…Cystatin A (CSTA) functions as a cysteine protease inhibitor by forming tight complexes with the cathepsins. …”
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Mechanistic insights into the recognition of Fe2+ and solvent-driven selectivity of chalcone-based receptors by Ajay Shankar

“…Spectrosc. 287 (2023) 122107; 303 (2023) 123129], this study offers novel insight into the supramolecular recognition of Fe2+ by receptor R1, wherein the conventional chalcone binding strategy is extended. Density Functional Theory (DFT) is used to differentiate the complexation behavior of chalcone-based receptors (R1, R2, R3), revealing key nucleophilic centres, particularly the carbonyl and phenolic oxygen atoms, as primary coordination sites. …”
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How (Ultra‐)Rare Gene Variants Improve Our Understanding of More Common Autoimmune and Inflammatory Diseases by Alexandre Belot, Maud Tusseau, Jade Cognard, Sophie Georgin‐Lavialle, Guilaine Boursier, Christian M. Hedrich

“…Additional monogenic diseases such as ELF4, WDR1 mutations and trisomy 8 further illustrate the genetic complexity of this condition. Observations from genetic studies in SLE and Behçet syndrome highlight the complexity of systemic inflammatory diseases in which distinct molecular defects caused by single‐gene mutations can promote lupus or Behçet syndromes, often unrecognizable from their genetically complex “classical” forms. …”
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Well Performance from Numerical Methods to Machine Learning Approach: Applications in Multiple Fractured Shale Reservoirs by Kailei Liu, Boyue Xu, Changjea Kim, Jing Fu

“…Horizontal well fracturing technology is widely used in unconventional reservoirs such as tight or shale oil and gas reservoirs. Meanwhile, the potential of enhanced oil recovery (EOR) methods including huff-n-puff miscible gas injection are used to further increase oil recovery in unconventional reservoirs. …”
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Static Clathrin Assemblies at the Peripheral Vacuole-Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia. by Jon Paulin Zumthor, Lenka Cernikova, Samuel Rout, Andres Kaech, Carmen Faso, Adrian B Hehl

“…Here, we investigated the molecular consequences of these adaptations on the Giardia endocytic machinery and membrane coat complexes. Despite the absence of canonical clathrin coated vesicles in electron microscopy, Giardia possesses conserved PV-associated clathrin heavy chain (GlCHC), dynamin-related protein (GlDRP), and assembly polypeptide complex 2 (AP2) subunits, suggesting a novel function for GlCHC and its adaptors. …”
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Evaluasi Kualitas Layanan terhadap Operator Telekomunikasi: Tinjauan Perspektif Pelanggan by Suryat Handoko, Nilda Tri Putri, Jonrinaldi Jonrinaldi

“…PT Semen Padang is now actively promoting synergies and pursuing the company's performance growth to be able to cope with competition in an increasingly tight and complex cement industry. In order to face this, it is necessary to have adequate human resource management from the company. …”
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THE OPTIMIZATION METHOD OF FUZZY AUTOMATIC CLASSIFICATION IN THE PROBLEM OF COMBINING THE ASSESSMENTS OF TRAJECTOR MEASUREMENTS IN THE RADAR SYSTEM by A. V. Khizhniak

“…By a radiolocation system the author mean an automated hierarchical technical complex that combines, using communication tools, a set of asynchronously functioning radiolocation tools, as well as central and intermediate points that collect, process and issue trajectory radiolocation information. …”
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BIOLOGICAL RESPONSE OF TREATMENT WITH SAFFRON PETAL EXTRACT ON CYTOKYNE-INDUCED OXIDATIVE STRESS AND INFLAMMATION IN THE CACO-2/THP1 COCOLTURE MODEL

“…Furthermore, the results of our model suggest that SPE treatment restores the functionality of the intestinal barrier by reducing the destruction of tight junctions induced by the inflammatory stimulus. …”
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