A guidebook for DISCO tissue clearing by Muge Molbay, Zeynep Ilgin Kolabas, Mihail Ivilinov Todorov, Tzu‐Lun Ohn, Ali Ertürk

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3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications by Qiqi Liu, Zhuo Song, Simin Liu, Zirui Dong, Xi Zheng, Tak Yeung Leung, Xiaoyan Chen

Subjects: “…tissue clearing…”
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Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice by Andrew W. Buxton, Frank L. Jalufka, Margaret E. Hruska, Jack R. Kubaney, Dylan A. McCreedy, Dylan A. McCreedy

Subjects: “…optical tissue clearing…”
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Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix by Kohei Togami, Hiroaki Ozaki, Yuki Yumita, Anri Kitayama, Hitoshi Tada, Sumio Chono

“…In this study, we compared techniques of staining the extracellular matrix with optical tissue-clearing treatment for developing three-dimensional imaging methods for focus sites in pulmonary fibrosis. …”
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Post-ejaculatory inhibition of female sexual drive via heterogeneous neuronal ensembles in the medial preoptic area by Kentaro K Ishii, Koichi Hashikawa, Jane Chea, Shihan Yin, Rebecca Erin Fox, Suyang Kan, Meha Shah, Zhe Charles Zhou, Jovana Navarrete, Alexandria D Murry, Eric R Szelenyi, Sam A Golden, Garret D Stuber

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Use of bovine serum albumin might impair immunofluorescence signal in thick tissue samples by Anna Chwastowicz, Artur Wolny, Magdalena Sobień, Marcin Barański, Jacek Tomczuk, Michał Szatkowski, Aleksandra Szredzka, Jakub Gołąb, Leszek Kaczmarek, Marzena Stefaniuk, Paweł Matryba

Subjects: “…Tissue clearing…”
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Osteoclast visualization: Tartrate-resistant acid phosphatase activity staining using NewFuchsin compatible with non-aqueous mounting and tissue clearing by Takashi Nakamura, Katsuhiro Kawaai, Yukiko Kuroda, Koichi Matsuo

“…. • The red dye formed by NewFuchsin TRAP staining can be detected not only in the bright field, but also in the fluorescent field. • Combined with tissue clearing using ethyl cinnamate, osteoclasts are observed using three-dimensional imaging.…”
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Whole‐brain microscopy reveals distinct temporal and spatial efficacy of anti‐Aβ therapies by Daniel Kirschenbaum, Ehsan Dadgar‐Kiani, Francesca Catto, Fabian F Voigt, Chiara Trevisan, Oliver Bichsel, Hamid Shirani, K Peter R Nilsson, Karl J Frontzek, Paolo Paganetti, Fritjof Helmchen, Jin Hyung Lee, Adriano Aguzzi

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ClearFinder: a Python GUI for annotating cells in cleared mouse brain by Stefan Pastore, Philipp Hillenbrand, Nils Molnar, Irina Kovlyagina, Monika Chanu Chongtham, Stanislav Sys, Beat Lutz, Margarita Tevosian, Susanne Gerber

Subjects: “…Tissue clearing…”
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Three-dimensional visualization and analysis of dendritic spines in human brain tissue by Haitao Sun, Hei Ming Lai, Wutian Wu

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Whole-brain 3D imaging of dopaminergic neurons and glial cells in the mouse model of Parkinson’s disease induced by 6-OHDA by Mengqi Wang, Linglong Xiao, Yifeng Shi, Yaping Wu, Xinyuejia Huang, Yang Wu, Yangyang Xu, Lin Bai, Wei Pan, Jie Zhang, Jie Zhang, Wei Wang

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Activity-dependent refinement of axonal projections forms one-to-one connection pattern in the developing chick ciliary ganglion by Ryo Egawa, Hiromu Yawo, Hiroshi Kuba

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Volumetric atlas of the rat inner ear from microCT and iDISCO+ cleared temporal bones by Daniele Cossellu, Elisa Vivado, Laura Batti, Ivana Gantar, Roberto Pizzala, Paola Perin

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The Role of Herb-Partitioned Moxibustion in the Angiogenesis of Colitis-Associated Cancer in Rats by Li K, Wu L, Zhu L, Wang W, Chen Y, Ma Z, Zhang G, Gu M, Zhang H, Wu H

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Sensory innervation in the prostate and a role for calcitonin gene-related peptide in prostatic epithelial proliferation by Hanyu Xia, Hanyu Xia, Travis J. Jerde, Travis J. Jerde, Jill C. Fehrenbacher, Jill C. Fehrenbacher, Jill C. Fehrenbacher, Jill C. Fehrenbacher

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Morphological segmentation with tiling light sheet microscopy to quantitatively analyze the three-dimensional structures of spinal motoneurons by Huijie Hu, Dongyue Wang, Yanlu Chen, Liang Gao

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Whole‐Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy by Yuezhou Wang, Zile Jiang, Kai Zhang, Huimin Tang, Guimei Wang, Jinshan Gao, Guanghui He, Baoyue Liang, Li Li, Chaoyong Yang, Xianming Deng

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Reduction of photo bleaching and long term archiving of chemically cleared GFP-expressing mouse brains. by Klaus Becker, Christian Markus Hahn, Saiedeh Saghafi, Nina Jährling, Martina Wanis, Hans-Ulrich Dodt

“…Tissue clearing allows microscopy of large specimens as whole mouse brains or embryos. …”
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Spiner, deep learning-based automated detection of spiral ganglion neurons in intact cochleae by Yuheng Cai, Greta M. Leitz-Najarian, Reuben Rosen, Kendall A. Hutson, Adele Moatti, Chen Li, Douglas C. Fitzpatrick, Alon Greenbaum

“…Summary: Tissue clearing and light-sheet fluorescence microscopy were applied for 3D profiling of intact cochleae. …”
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The Blood-Brain Barrier in Both Humans and Rats: A Perspective From 3D Imaging by Aiwen Chen, Gavin Volpato, Alice Pong, Emma Schofield, Jun Huang, Zizhao Qiu, George Paxinos, Huazheng Liang

“…Conclusions: The present study demonstrated the 3D structure of both the human and rat BBB, which is discrepant from the 2D one. Tissue clearing and 3D imaging are promising techniques to answer more questions about the real structure of biological specimens.…”
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