Problems of creating antibody phage libraries and their solutions by V. S. Aripov, N. V. Volkova, A. A. Ilyichev, D. N. Shcherbakov

“…After a helper phage is added to the suspension of transformed E. coli cells, a bacteriophage library is formed, which is a working tool for performing the affinity selection procedure and searching for individual molecules. …”
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Antimicrobial Susceptibility Testing for Colistin: Extended Application of Novel Quantitative and Morphologic Assay Using Scanning Electron Microscopy by Omar Zmerli, Sara Bellali, Gabriel Haddad, Rim Iwaza, Akiko Hisada, Erino Matsumoto, Yusuke Ominami, Didier Raoult, Jacques Bou Khalil

“…Forty-four Gram-negative reference organisms were chosen based on their Colistin susceptibility profiles. Bacterial suspensions of ∼107 CFU/mL were exposed to Colistin at EUCAST-ECOFF, with controls not exposed, incubated at 37°C, and then sampled at 0, 15, 30, 60, and 120 minutes. …”
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Cortistatin-14 Exerts Neuroprotective Effect Against Microglial Activation, Blood-brain Barrier Disruption, and Cognitive Impairment in Sepsis-associated Encephalopathy by Qiang Wen, Qian Ding, Jinchao Wang, Yanhua Yin, Shangchen Xu, Yuanrong Ju, Hongsheng Ji, Bin Liu

“…The novel object recognition test (NORT), open field test (OFT), elevated plus maze test (EPMT), and tail suspension test (TST) were used to explore the behavioral performance of the mice. …”
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Design and Fabrication of a Car Sports Rims. by Nsimbe Laban

“…Car rims are however required to be as light as possible so as to achieve more performance advantages such as quick acceleration and stopping, increased fuel efficiency and less strain on suspension components. The density of steel is higher than that of aluminium alloys thus steel is heavier as compared to aluminium alloys. …”
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Flow Cytometry of the Side Population: Tips & Tricks by Irene Sales-Pardo, Ariadna Avendaño, Vanessa Martinez-Muñoz, Marta García-Escarp, Raquel Celis, Phil Whittle, Jordi Barquinero, Joan Carles Domingo, Pedro Marin, Jordi Petriz

“…Methods: First of all and mainly for the SP application, the laser beam paths were exhaustively checked to ensure the lowest coefficients of variation. Blood suspensions were prepared by erythrocyte lysis with ammonium chloride and hematopoietic cells were labeled with Ho342. …”
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Evaluation of a user‐friendly CSDS cage apparatus for studying depressive‐like behaviors in rodents by Hao Zhang, Dongmei Gao, Minghu Hu, Wanqing Zhou, Muxuan Han, Ya Sun, Yang Zhang, Jieqiong Wang, Mingzhou Gao

“…To evaluate the effectiveness of the newly developed tools, a variety of animal models, including the tail suspension test (TST), sucrose preference test, forced swimming test (FST), novelty‐suppressed feeding test, female urine sniffing test, and open field test (OFT), were adopted to assess depressive‐like behaviors in mice. …”
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Naked-Eye Molecular Testing for the Detection of <i>Xylella fastidiosa</i> in Mallorca (Balearic Island) Almond Orchards by Colorimetric LAMP by Amoia Serafina Serena, Ana Falcón-Piñeiro, Milica Pastar, José Manuel Garcìa-Madero, Nicoletta Contaldo, Mikael Muegge, Stéphane Compant, Pasquale Saldarelli, Angelantonio Minafra

“…The cLAMP assay was able to detect up to 100 CFU/mL of the <i>Xf</i> bacterial suspension diluted in healthy almond sap. The same crude extracts used in the cLAMP test were also tested by qPCR. …”
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Concept of producing of the Russian national system of functional food by Yu. V. Fotev, V. F. Pivovarov, A. M. Artemyeva, I. M. Kulikov, Y. K. Goncharova, А. I. Syso, N. P. Goncharov

“…One way to reduce the negative consequences is through the introduction and breeding of new for Russia species and varieties of agricultural plants and organize a mass production of functional foods (FF) that contribute to the prevention and/or suspension of the development of dangerous diseases and slowing down the aging process. …”
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Perspectives of push-pull-mooring effects on a desire for switching to alternative crops among tobacco farmers in Thailand: A qualitative study by Chakkraphan Phetphum, Artittaya Wangwonsin, Atchara Prajongjeep, Saksin Simsin

“…The fourth category focused on the support needed for a successful transition, including the suspension of tobacco debt payments, access to low-interest loans, and the development of marketing capabilities. …”
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A study on the differences in the gut microbiota and metabolism between male and female mice in different stress periods by Yajun Qiao, Yajun Qiao, Yajun Qiao, Juan Guo, Qi Xiao, Jianv Wang, Jianv Wang, Xingfang Zhang, Xingfang Zhang, Xinxin Liang, Xinxin Liang, Lixin Wei, Lixin Wei, Hongtao Bi, Tingting Gao, Tingting Gao

“…In this study, chronic restraint stress (CRS) mouse models were used to simulate chronic stress, and behavioral tests were conducted, including the open field test (OFT), tail suspension test (TST) and forced swimming test (FST). …”
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Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection by Pavlo Virych, Nadiia Shuvalova, Anton Karas, Galina Karas, Svitlana Chaika, Tetiana Kucherenko, Ganna Minina, Marina Timchenko, Oleg Melnykov, Yurii Minin

“…A previously prepared suspension of homologous mesenchymal stem cells (5 million) in 0.5 ml physiological solution was injected into the vein of the opposite ear. …”
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Phytochemical screening and neuro-pharmacological activity of Mimosa pudica flowers: Integrating in vitro, in silico and in vivo approaches by Fahmida Alam, Rashedul Alam, A.T.M. Yusuf, Joya Datta Ripa, Raktim Das Nithin, Sourav Barua, Mohammed Fazlul Kabir, Seong-Tshool Hong, Hea-Jong Chung

“…These tests included the open field test, light-dark box test, elevated plus maze test, tail suspension test, forced swim test, Y-maze test, hole cross test, and social interaction test. …”
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Suppression Efficiency for Dust from an Iron Ore Pile Using a Conventional Sprinkler and a Water Mist Generator by Yen-Yi Lee, Chung-Shin Yuan, Po-Hsuan Yen, Justus Kavita Mutuku, Chien-Er Huang, Chih-Cheng Wu, Po-Jen Huang

“…Abstract Re-suspension of iron ore dust presents a constant hazard in the working environment within steel production plants. …”
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Comparative drug release kinetics of Terminalia arjuna mediated SeNPs NanoGel and ZnONPs NanoGel – An in-vitro study by R. Vijayalakshmi, N. Ambalavanan, S. Rajeshkumar, Jaideep Mahendra, Uma Sudhakar, Devi Parameswari

“…Each sample, approximately 0.1 mg, was mixed with 10 mL of phosphate buffer saline (PBS) at various pH levels and maintained at 37 °C. The suspension was then placed in an incubated shaker at 120 rpm for 1 h. …”
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BVDV comprehensive studies and species identification in high-yielding livestock populations in the Sverdlovsk Oblast by N. A. Bezborodova, V. V. Kozhukhovskaya, E. V. Pechura, N. A. Martynov, O G. Tomskikh, A. N. Vasilyeva

“…Based on typing results, the virus isolates detected in four samples (nasopharyngeal swabs of calves, suspension prepared from aborted fetus organs and placenta) were classified as BVDV-1 virulent genotypes. …”
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Design, formulation and antimicrobial assessment of a traditional Iranian antiperspirant formula from Rosa damascena flowers and Myrtus communis leaves. by Maryam Alipanah, Gholamhossein Yousefi, Zahra Sobhani, Mohammad Ali Jahromi, Mohammad Zarshenas

“…Most of the resulting formulated suspension products showed consistency in texture, a light brown appearance, and a pH value of 5.15. …”
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Gene regulatory patterning codes in early cell fate specification of the C. elegans embryo by Alison G Cole, Tamar Hashimshony, Zhuo Du, Itai Yanai

“…Previous single-cell RNA-Seq studies in C. elegans have analyzed cells from a mixed suspension of cells from many embryos to study late differentiation stages, or individual early stage embryos to study early gene expression in the embryo. …”
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Preparation of noninfectious scRNAseq samples from SARS-CoV-2-infected epithelial cells. by Raven M Osborn, Justin Leach, Michelle Zanche, John M Ashton, ChinYi Chu, Juilee Thakar, Stephen Dewhurst, Sonia Rosenberger, Martin Pavelka, Gloria S Pryhuber, Thomas J Mariani, Christopher S Anderson

“…Briefly, we infected African green monkey kidney epithelial cells (Vero-E6) with SARS-CoV-2 for 96 hours, trypsin-dissociated the cells, and inactivated them with methanol-acetone in a single-cell suspension. Fixed cells were tested for the presence of infectious SARS-CoV-2 virions using the Tissue Culture Infectious Dose Assay (TCID50), and also tested for viability using flow cytometry. …”
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Colonic Transendoscopic Enteral Tubing: Route for a Novel, Safe, and Convenient Delivery of Washed Microbiota Transplantation in Children by Min Zhong, Heena Buch, Quan Wen, Chuyan Long, Bota Cui, Faming Zhang

“…No discomfort was reported during injection of the microbiota or medication suspension through the TET tube. During the follow-up, no severe adverse events were observed. …”
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Application Study on Active Advanced Support Technology in Deep Roadway under Mine Goaf by Pengfei Jiang, Peng Xiao, Fanbao Meng, Suolin Jing, Jingkai Zhang, Ge Wang, Peng Zhao

“…Under the condition of overlying goaf, there was a complete stress structure, which can meet the requirements of suspension support.…”
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