Synchronized Visualization and Analysis of Intracellular Trafficking and Maturation of Orthoflavivirus Subviral Particles by Kotaro Ishida, Eiji Morita

“…To visualize intracellular membrane trafficking, we combine two distinct imaging techniques: the retention using selective hooks (RUSH) system and the split green fluorescent protein (GFP) system. In this approach, GFP11, a peptide tag fused to prME, the outer coat structural protein of Japanese encephalitis virus particles, was co-expressed in HeLa cells along with two additional components: GFP1-10 fused to a streptavidin-binding peptide and a hook construct consisting of streptavidin fused to the ER retention sequence KDEL. …”
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