Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating Plant Cardamine hupingshanesis by Xinzhao Tong, Linxi Yuan, Lei Luo, Xuebin Yin

“…Phylogenetic analysis and 16S rRNA gene sequences determined the strain as a kind of Gram positive bacillus and belonged to the family Brevibacterium frigoritolerans. …”
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Decreasing of heterogeneity in black tiger prawn (Penaeus monodon) and white shrimp (Litopenaeus vannamei) broodstocks and their fry produced in hatchery by Bambang Widyo Prastowo, Rahayu Rahardianti, Evi Maftuti Nur

“…While the primers for white shrimp was based on COIL and COIH sequences, and their PCR products were digested with Nla III.  …”
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The Geometric Series Hypothesis of Leaf Area Distribution and Its Link to the Calculation of the Total Leaf Area per Shoot of <i>Sasaella kongosanensis</i> ‘Aureostriatus’ by Yong Meng, David A. Ratkowsky, Weihao Yao, Yi Heng, Peijian Shi

“…This work demonstrates that the leaf area sequence per shoot follows a GS and provides a useful tool for the calculation of total leaf area per shoot, which is helpful to assess the photosynthetic capacity of plants.…”
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Treatment failure and drug resistance among the newly reported HIV-infected patients in Taizhou City from 2020 to 2022 by LI Yan, WU Xuanhe, SHI Lu, LI Guixia, WANG Shanling, WANG Yating, CHEN Tailin, WANG Tingting, XIE Yali, SHEN Weiwei, LIN Haijiang, CHEN Xiaoxiao, HE Na

“…Among the 36 HIV-infected patients with virological failure, 27 sequences were successfully amplified, with a successful amplification rate of 75.0% (27/36). …”
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<i>shp-2</i> gene knockout upregulates CAR-driven cytotoxicity of YT NK cells by V. G. Subrakova, S. V. Kulemzin, T. N. Belovezhets, A. N. Chikaev, N. A. Chikaev, O. A. Koval, A. A. Gorchakov, A. V. Taranin

“…Here, we took advantage of the CRISPR/Cas9 system to introduce mutations in the coding sequence of the shp-2 (PTPN11) gene encoding the signaling molecule of inhibitory pathways in NK cells. …”
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Genome-Wide Analysis of the <i>PLATZ</i> Gene Family in <i>Oryza</i> Genus: Evolution, Expression During Inflorescence Development and Stress Responses by Hongwei Chen, Xiufang Ma, Guilan Lv, Zheng Wang, Lili Wang, Bowen Yan, Wenqi Shang, Xianju Wang, Zuobin Ma, Wenjing Zheng

“…Phylogenetic analysis revealed six distinct groups among these genes, with high sequence similarity among members indicating a common evolutionary origin and potential functional conservation. …”
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Diagnosis and Treatment of Irritable Bowel Syndrome: Clinical Recommendations of the Russian Gastroenterological Association and Association of Coloproctologists of Russia by V. T. Ivashkin, I. V. Maev, Yu. A. Shelygin, E. K. Baranskaya, S. S. Belous, E. A. Belousova, A. G. Beniashvili, S. V. Vasilyev, A. V. Veselov, E. G. Grigoryev, N. V. Kostenko, V. N. Kashnikov, V. F. Kulikovskiy, I. D. Loranskaya, O. S. Lyashenko, E. A. Poluektova, V. G. Rumyantsev, V. M. Timerbulatov, O. Yu. Fomenko, D. A. Khubezov, E. Yu. Chashkova, G. I. Chibisov, M. V. Shapina, A. A. Sheptulin, O. S. Shifrin, A. S. Trukhmanov, O. P. Alekseeva, S. A. Alekseenko, A. Yu. Baranovsky, O. Yu. Zolnikova, N. V. Korochanskaya, S. N. Mammayev, I. B. Khlynov, V. V. Tsukanov

“…Due to challenging differential diagnosis, IBS can be appropriately established per exclusionem, with pre-examination as follows: general and biochemical blood tests; tissue transglutaminase IgA/IgG antibody tests; thyroid hormones test; faecal occult blood test; hydrogen glucose/ lactulose breath test for bacterial overgrowth; stool test for enteric bacterial pathogens and Clostridium difficile A/B toxins; stool calprotectin test; abdominal ultrasound; OGDS, with biopsy as appropriate; colonoscopy with biopsy. The IBS sequence is typically wavelike, with alternating remissions and exacerbations often triggered by psychoemotional stress. …”
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Testing the Breakdown of the Asteroseismic Scaling Relations in Luminous Red Giants by Amanda L. Ash, Marc H. Pinsonneault, Mathieu Vrard, Joel C. Zinn

“…We test the ${\nu }_{{\rm{\max }}}$ and Δ ν scaling relations separately, by using stars of known mass and radius in star clusters and the Milky Way's high- α sequence. We find evidence that the Δ ν scaling relation contributes to the observed breakdown in luminous giants more than the ${\nu }_{{\rm{\max }}}$ relation. …”
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A hybrid setup for rodent hyperpolarized metabolic imaging using a clinical magnetic resonance scanner by Ditte Bentsen Christensen, Ingeborg Sæten Skre, Jan Henrik Ardenkjær-Larsen, Mor Mishkovsky, Mathilde H Lerche

“…We detail the experimental procedure, provide practical guidance, and showcase the capabilities of the system with example data from mouse brain imaging.This hybrid setup bridges the gap between clinical and pre-clinical research, enabling iterative testing of equipment, imaging sequences, and hypotheses across phantoms, in vivo rodent models and clinical settings. …”
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SERS Platform Based on Bimetallic Au-Ag Nanowires-Decorated Filter Paper for Rapid Detection of miR-196ain Lung Cancer Patients Serum by Ji Xia, Yifan Liu, Menglin Ran, Dan Lu, Xiaowei Cao, Youwei Wang

“…Studies on nonspecific sequence and single-base mismatch of miRNA demonstrated that SERS-based platform was highly selective, excellent uniform, and reproducible. …”
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EPIMR: Prediction of Enhancer-Promoter Interactions by Multi-Scale ResNet on Image Representation by Qiaozhen Meng, Yinuo Lyu, Xiaoqing Peng, Junhai Xu, Jijun Tang, Fei Guo

“…First, Hilbert Curve is utilized to represent sequences to images to preserve the position and spatial information. …”
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De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer <i>Azohydromonas lata</i> Strain H1 Genome and Genomic Analysis of PHB Production Machinery by Daniele Traversa, Carlo Pazzani, Pietro D’Addabbo, Lucia Trisolini, Matteo Chiara, Marta Oliva, Angelo Marzella, Camilla Mandorino, Carla Calia, Guglielmina Chimienti, Caterina Manzari, Graziano Pesole, Maria Scrascia

“…A comparative genomic analysis of the available genomes of the genus <i>Azohydromonas</i> revealed the presence of <i>phaCAB</i> and auxiliary genes in all <i>Azohydromonas</i> species investigated, suggesting that the PHB production is a common feature of the genus. Based on sequence identity, we also suggest <i>A. australica</i> as the closest species to which the <i>phaCAB</i> operon of the strain H1, reported in 1998, is similar.…”
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Machine learning and genetic algorithm-guided directed evolution for the development of antimicrobial peptides by Heqian Zhang, Yihan Wang, Yanran Zhu, Pengtao Huang, Qiandi Gao, Xiaojie Li, Zhaoying Chen, Yu Liu, Jiakun Jiang, Yuan Gao, Jiaquan Huang, Zhiwei Qin

“…Methods: LBDA-D was identified as an output of this algorithm, in which the original LBDMj sequence was the input, and the three-dimensional solution structure of LBDB was determined using nuclear magnetic resonance. …”
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Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein by Wenhui Lu, Baoli Sun, Jianyue Mo, Xiduo Zeng, Guanqun Zhang, Lianxiang Wang, Qingfeng Zhou, Ling Zhu, Zhili Li, Qingmei Xie, Yinzuo Bi, Jingyun Ma

“…Furthermore, we determined the whole genome sequences of these four intermediate viruses. The results showed that after 100 passages, compared to QY1 P5, a total of 32 amino acid mutations were found. …”
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Neural Network-Based Parameter Estimation and Compensation Control for Time-Delay Servo System of Aeroengine by Hongyi Chen, Qiuhong Li, Zhifeng Ye, Shuwei Pang

“…The estimated delay was embedded into an online sequential extreme learning machine, and a nonlinear model predictive controller was designed to obtain an optimal control sequence. The compensation control loop was designed to reduce the impact of the model and delay mismatch problems of the control system. …”
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Applying Focal Therapy to Lesions Detected via Magnetic Resonance Imaging: Delivering Cancer Ablation Beyond the Visibility Phenomenon by Clement Orczyk, Teresa Marsden, Francesco Giganti, Joseph M. Norris, Santosh Waigankar, Louise Dickinson, Shonit Punwani, Alex Kirkham, Alex Freeman, Aiman Haider, Caroline M. Moore, Arnauld Villers, Clare Allen, Mark Emberton

“…We propose a 10-step framework that incorporates the contribution of all MRI sequences, the cancer growth pattern within the zonal anatomy to establish a margin around the MRI-visible lesion, safeguards for critical anatomic structures, and guidance for energy selection on the basis of specific properties. …”
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Small open reading frames (sORFs): Driving big improvements in plant development and quality by Kui Dong, Chaofan Shan, Dongyu Wen, Zifan Cui, Jun Cao

“…Small open reading frames (sORFs) are defined as short nucleotide sequences, typically no longer than 300 base pairs, which have the potential to encode small peptides. …”
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Understanding the impacts of dual methionine oxidations in complementarity-determining regions on the structure of monoclonal antibodies by Bo Zhao, Joy Yoon, Bojie Zhang, Youmi Moon, Yue Fu, Yinyin Li, Yunlong Zhao, Hui Xiao, Ning Li

“…Moreover, the presence of multiple methionines in close proximity within the amino acid sequence increases the complexity of accurate characterization, and sophisticated analytical methods are required to detect these modifications. …”
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Cigarette Smoking Is Associated with Prolongation of the QTc Interval Duration in Patients with Type 2 Diabetes Mellitus by Petros Thomakos, Stavros Liatis, Stavroula Kalopita, Ioannis Vlahodimitris, Chryssoula Stathi, Nicholas Katsilambros, Nicholas Tentolouris, Konstantinos Makrilakis

“…In addition, baroreflex sensitivity (BRS) was evaluated using the spontaneous sequence method. The mean QTc interval was measured from the 24-hour ECG recordings. …”
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Genotypic Characterization of Azotobacteria Isolated from Argentinean Soils and Plant-Growth-Promoting Traits of Selected Strains with Prospects for Biofertilizer Production by Esteban Julián Rubio, Marcela Susana Montecchia, Micaela Tosi, Fabricio Darío Cassán, Alejandro Perticari, Olga Susana Correa

“…The genetic diversity among 31 putative Azotobacter isolates obtained from agricultural and non-agricultural soils was assessed using rep-PCR genomic fingerprinting and identified to species level by ARDRA and partial 16S rRNA gene sequence analysis. High diversity was found among the isolates, identified as A. chroococcum, A. salinestris, and A. armeniacus. …”
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