Molecular Characterization and Expression of <i>unc-13d</i> in the Sex Reversal of <i>Monopterus albus</i> by Zitong Lian, Fang Meng, Xueping Xia, Junchao Fang, Haifeng Tian, Qiaomu Hu

“…In this study, we firstly cloned and characterized the sequence and protein structure of <i>unc-13d</i> of <i>M. albus.…”
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Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia by Teguh Hari Sucipto, Tomohiro Kotaki, Kris Cahyo Mulyatno, Siti Churrotin, Amaliah Labiqah, Soegeng Soegijanto, Masanori Kameoka

“…Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method. 17 out of 359 blood samples (4.7%) were positive for the isolation of DENV. …”
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Mutation Analysis of HTRA2 Gene in Chinese Familial Essential Tremor and Familial Parkinson’s Disease by Ya-Chao He, Pei Huang, Qiong-Qiong Li, Qian Sun, Dun-Hui Li, Tian Wang, Jun-Yi Shen, Juan-Juan Du, Shi-Shuang Cui, Chao Gao, Rao Fu, Sheng-Di Chen

“…Methods. We directly sequenced all eight exons, exon-intron boundaries, and part of the introns in 101 familial essential tremor patients, 105 familial Parkinson’s disease patients, and 100 healthy controls. …”
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Advances in bioinformatics and multi-omics integration: transforming viral infectious disease research in veterinary medicine by Alyaa Elrashedy, Walid Mousa, Mohamed Nayel, Akram Salama, Ahmed Zaghawa, Ahmed Elsify, Mohamed E. Hasan

“…With significant advancements in bioinformatics and NGS, researchers can now identify contigs, which are contiguous sequences of DNA that are assembled from overlapping fragments, assemble a complete genome, perform phylogenetic analysis to diagnose, investigate the risk of viral diseases in animals, handle and share large biological datasets across various species. …”
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Prevalence and phylogenetic analysis of Mycobacterium avium subsp. paratuberculosis in cattle in Mosul city, Iraq by Sadam D. Hasan, Mohammad A. Altaliby, Amer H. Taha, Karam M. Abdulrazzaq

“…Furthermore, in this study, two sequences of Mycobacterium avium subsp. paratuberculosis were deposited in GenBank (PP976335.1 and PP976335.1); phylogenetically the study strains showed 100% identity near MAP strain in Saudi Arabia under the accession number (MN928512.1) and 99.85% identity with Spain, Germany and South Korea strain accession numbers (FJ775181.1, CP053068.1 and CP033909.1) respectively. …”
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Explore human parsing modality for action recognition by Jinfu Liu, Runwei Ding, Yuhang Wen, Nan Dai, Fanyang Meng, Fang‐Lue Zhang, Shen Zhao, Mengyuan Liu

“…Abstract Multimodal‐based action recognition methods have achieved high success using pose and RGB modality. However, skeletons sequences lack appearance depiction and RGB images suffer irrelevant noise due to modality limitations. …”
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Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis by Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Ouyang Lizhi, Parthasarathy Ranganathan, Nsoki Phambu, Alexander J. Ropelewski, Theodore W. Thannhauser

“…In previous work, we reported on the isolation and genome sequence analysis of Bacillus cereus strain tsu1 NCBI accession number JPYN00000000. …”
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Fire Resistance of Composite Beams with Restrained Superposed Slabs by Junli Lyu, Qichao Chen, Huizhong Xue, Yongyuan Cai, Jingjing Lyu, Shengnan Zhou

“…ABAQUS was employed to simulate the temperature fields and deformation behaviours of composite beam specimens based on a sequenced thermomechanical coupling analysis. The numerical results agreed well with the experiment data, which validated the developed numerical model.…”
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Engineering hydrophobicity and manufacturability for optimized biparatopic antibody–drug conjugates targeting c-MET by Andreas Evers, Simon Krah, Deniz Demir, Ramona Gaa, Desislava Elter, Christian Schroeter, Stefan Zielonka, Nicolas Rasche, Julia Dotterweich, Christine Knuehl, Achim Doerner

“…The methodologies of in silico hydrophobicity identification and sequence optimization could serve as a blueprint for rapid development of optimal biparatopic ADCs targeting further tumor-associated antigens in the future.…”
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Single dose of recombinant baculovirus vaccine expressing sigma B and sigma C genes provides good protection against novel duck reovirus challenge in ducks by Yufei Wang, Siyi Xu, Xia Chen, Yunlong Dou, Xingzhu Yang, Zenglei Hu, Shuang Wu, Xiaoquan Wang, Jiao Hu, Xiufan Liu

“…Two recombinant viruses, namely rBac-σB and rBac-σC, were successfully generated based on the consensus sequence of NDRV. Then, the σB and σC subunit vaccine candidates were prepared by directly inactivating the recombinant virus infected-Sf9 cell suspension. …”
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Case Study of Somaclonal Variation in Resistance Genes Mlo and Pme3 in Flaxseed (Linum usitatissimum L.) Induced by Nanoparticles by Inese Kokina, Ilona Mickeviča, Marija Jermaļonoka, Linda Bankovska, Vjačeslavs Gerbreders, Andrejs Ogurcovs, Inese Jahundoviča

“…In this paper, it was essential to obtain DNA material from all tissue culture development stages from one donor plant to record changes in each nucleotide sequence. Gene region specific primers were developed for resistance genes such as Mlo and Pme3 to define the genetic variability in tissue culture of L. usitatissimum. …”
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Multispanning membrane protein SIDT2 increases knockdown activity of gapmer antisense oligonucleotides by Kohshi Kusumoto, Kiyomi Sasaki, Yasunori Uchida, Ayaka Utsumi, Tokuyuki Yoshida, Satoshi Obika, Takao Inoue, Keiichiro Okuhira

“…In this study, we conducted a comparative analysis of the amino acid sequences of mammalian SIDT1 and SIDT2 to identify regions characteristic to each. …”
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A computational tool to infer enzyme activity using post-translational modification profiling data by Dehui Kong, Aijun Zhang, Ling Li, Zuo-Fei Yuan, Yingxue Fu, Long Wu, Ashutosh Mishra, Anthony A. High, Junmin Peng, Xusheng Wang

“…In addition, JUMPsem is capable of establishing novel enzyme-substrate relationships through searching motif sequences. JUMPsem outperforms widely used kinase activity tools, such as IKAP and KSEA, in terms of the number of kinases and the computational speed. …”
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PCR and ELISA vis-à-vis Microscopy for Detection of Bovine Anaplasmosis: A Study on Associated Risk of an Upcoming Problem in North India by Amrita Sharma, L. D. Singla, Paramjit Kaur, M. S. Bal

“…Five false negative samples by msp1β PCR were reconfirmed for Anaplasma spp. targeting 16S rRNA gene. The sequence analysis showed the presence for A. marginale specific restriction site, indicating variation in the local strains of the organism resulting in no amplification with msp1β gene primers. …”
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Targeting the epigenome with advanced delivery strategies for epigenetic modulators by Sonia Guha, Yogeswaran Jagadeesan, Murali Monohar Pandey, Anupama Mittal, Deepak Chitkara

“…By modulating the epigenetic status, the expression of genes can be regulated without any change in the DNA sequence itself. Epigenetic drugs exhibit promising therapeutic efficacy against several epigenetically originated diseases including several cancers, neurodegenerative diseases, metabolic disorders, cardiovascular disorders, and so forth. …”
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Smart Cognitive HMI With Automated Knowledge Extraction for Machine Tool by Jongsu Park, Jinho Son, Seongwoo Cho, Jumyung Um

“…It provides relevant manual context, machine status, current operation, and operation sequence, depending on the users&#x2019; intention. …”
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Automatic monitoring of activity intensity in a chicken flock using a computer vision-based background image subtraction technique: An experimental infection study with fowl adenov... by Hiroshi Iseki, Eri Furukawa, Tomoya Shimasaki, Shogo Higaki

“…The foreground (moving object: chicken) was extracted from the videos using background subtraction, and moving pixels were counted in 5-frame-per-second sequences. Analysis of hourly averaged moving pixel ratios (total pixels of the moving object / total pixels of the region of interest) showed that the daytime top-view video was suitable. …”
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The Chloroplastic Small Heat Shock Protein Gene KvHSP26 Is Induced by Various Abiotic Stresses in Kosteletzkya virginica by Xiaohua Liu, Lizi Zhao, Jianzhao Li, Lijun Duan, Kai Zhang, Xuqiang Qiao, Weihuan Li, Chengchao Zheng, Xiaoli Tang, Hongxia Zhang

“…Bioinformatics analyses revealed that KvHSP26 encoded a chloroplastic protein with the typical features of sHSPs. Amino acid sequence alignment and phylogenetic analysis demonstrated that KvHSP26 shared 30%-77% homology with other sHSPs from Arabidopsis, cotton, durian, salvia, and soybean. …”
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Coloring hot-dip galvanization of steel samples in industrial zinc-manganese baths by Godzsák M., Lévai G., Vad K., Csik A., Hakl J., Kulcsár T., Kaptay G.

“…The obtained colors change in the sequence blue - yellow - pink - green with increasing the Mn-content of the bath and with increasing the wall thickness of the steel samples. …”
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Numerical investigation of welding deformation diminution for double shell structure using the layered inherent strain by Cheng Li, Hua Zhai, Lianwei Zhu, Zhihong Liu, Jianguo Ma, Xiaofeng Zhu, Qiong Liu

“…Numerical simulation and experimental studies were conducted on the welding deformation of the double shell specimen, and the welding sequence and design of the welding fixture were discussed in detail. …”
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