Diverse vaginal microbiota in healthy Japanese women: a combined relative and quantitative analyses by Masahiro Ito, Misaki Kataoka, Yoichi Sato, Hideki Nachi, Koji Nomoto, Koji Nomoto, Nobuhiko Okada

“…DNA was extracted from the vaginal fluid, and the 16S ribosomal RNA gene sequences in the V3-V4 regions were analyzed to identify bacterial species. …”
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Combining Region-Guided Attention and Attribute Prediction for Thangka Image Captioning Method by Fujun Zhang, Wendong Kang, Wenjin Hu

“…Additionally, we designed a Thangka feature enhancement encoder to further refine the regional feature vectors, which are then fused with global features extracted by CLIP through multi-scale convolutional fusion, injecting richer object-related information into the Thangka image features.Furthermore, to enhance the detailed representation capability for generating long-sequence captions of Thangka images, we designed an attribute predictor. …”
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Molecular Detection of FIPV among Imported Felines through Soekarno Hatta Airport, Indonesia by Fidyah Fitrawati, Suwarno, Ira Sari Yudaniayanti, Jola Rahmahani, Muchammad Yunus, Soeharsono, Desniwaty Karo-karo, Haeriah

“…Two positive samples, namely 123v and 682v, were further sequenced. The study results indicate that the FCoV virus can be found in asymptomatic imported cats. …”
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Role of p16INK4A in Replicative Senescence and DNA Damage-Induced Premature Senescence in p53-Deficient Human Cells by Razmik Mirzayans, Bonnie Andrais, Gavin Hansen, David Murray

“…The p16INK4A (hereafter p16) tumor suppressor is encoded by the INK4A/ARF locus which is among the most commonly dysregulated sequences in human cancer. By inhibiting cyclin-dependent kinases, p16 activates the G1-S checkpoint, and this response is often considered to be critical for establishing a senescence-like growth arrest. …”
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Dual Strategy Based Improved Swarm Intelligence and Stacked LSTM With Residual Connection for Land Use Land Cover Classification by Vinaykumar Vajjanakurike Nagaraju, Ananda Babu Jayachandra, Andrzej Stateczny, Swathi Holalu Yogesh, Raviprakash Madenur Lingaraju, Balaji Prabhu Baluvaneralu Veeranna

“…By integrating residual connection with stacked LSTM, gradient flow is enabled directly among long sequences, reducing the vanishing gradient issue and fastening the convergence rate. …”
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Investigating the Lower Limit of Metallicity for the Galactic Thin Disk by Guozhen Hu, Zhengyi Shao, Erbil Gügercinoǧlu, Wenyuan Cui

“…We explore the metal-poor regime of the Galactic disk on the distribution of stars in the [ α /M]– V _ϕ plane, to identify the most metal-poor thin disk (MPTnD) stars belonging to the low- α sequence. Chemical abundances and velocities of sample stars are either taken or derived from APOGEE DR17 and Gaia DR3 catalogs. …”
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Electrochemical detection of human papillomavirus DNA based on an ultrasensitive electrochemical sensing platform using N-graphene paper by Jia Wang, Juelan Ye, Gang Li

“…This work addresses this critical need by developing an ultrasensitive electrochemical biosensing platform based on nitrogen-doped graphene paper electrodes for quantifying high-risk HPV16 DNA sequences. The N-graphene nanohybrid, fabricated via simple solvothermal treatment of biomass-derived graphene oxide, demonstrated significantly enhanced electrical and electrocatalytic properties compared to undoped graphene. …”
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Comparative Genomic In Situ Hybridization and the Possible Role of Retroelements in the Karyotypic Evolution of Three Akodontini Species by Naiara Pereira Araújo, Gustavo Campos Silva Kuhn, Flávia Nunes Vieira, Thaís Queiroz Morcatty, Adriano Pereira Paglia, Marta Svartman

“…FISH was also performed to assess the distribution of telomeric sequences, long and short interspersed repetitive elements (LINE-1 and B1 SINE) and of the endogenous retrovirus mysTR in the genomes of the three species. …”
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Isolation and Characterization of Paracoccus sp. GSM2 Capable of Degrading Textile Azo Dye Reactive Violet 5 by Mallikarjun C. Bheemaraddi, Santosh Patil, Channappa T. Shivannavar, Subhashchandra M. Gaddad

“…A potential bacterial strain GSM2, capable of degrading an azo dye Reactive Violet 5 as a sole source of carbon, was isolated from textile mill effluent from Solapur, India. The 16S rDNA sequence and phenotypic characteristics indicated an isolated organism as Paracoccus sp. …”
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Synthesis, DNA-Binding, Anticancer Evaluation, and Molecular Docking Studies of Bishomoleptic and Trisheteroleptic Ru-Diimine Complexes Bearing 2-(2-Pyridyl)-quinoxaline by Sofia Balou, Athanasios Zarkadoulas, Maria Koukouvitaki, Luciano Marchiò, Eleni K. Efthimiadou, Christiana A. Mitsopoulou

“…The experimental data were confirmed by molecular docking studies, employing two different DNA sequences. Both complexes, 1 and 2, bind with DNA via a minor groove mode of binding. …”
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Land Subsidence in the Yangtze River Delta, China Explored Using InSAR Technique From 2019 to 2021 by Hongbo Jiang, Guangcai Feng, Yuexin Wang, Zhiqiang Xiong, Hesheng Chen, Ning Li, Zeng Lin

“…To overcome these challenges, we adopted the multitemporal InSAR technique, integrating a frame data parallel processing strategy and an overall adjustment correction method, to obtain the temporal deformation sequences of the entire Yangtze River Delta (YRD) region in China from January 2019 to December 2021. …”
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Research on High-Frequency Combination Coding-Based SSVEP-BCIs and Its Signal Processing Algorithms by Feng Zhang, Chengcheng Han, Lili Li, Xin Zhang, Jun Xie, Yeping Li

“…Lastly, the differentiation combination (DC) method is proposed to select the combination coding sequence in order to increase the recognition rate; as a result, IHHT algorithm and DC method for the proposed SSVEP paradigm in this study increase recognition efficiency so as to improve ITR and increase the stability of the BCI system. …”
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Experimental and discrete element method study on the mechanical properties of hole-fracture sandstone by Peijie Lou, Peijie Lou, Peijie Lou, Peijie Lou, Lichen Sun, Cheng Pan, Penghui Ji, Huan Liu

“…The formation of micro-fractures progressively adhered to a “clusteringexpansion-coalescence” sequence along the paths of the hole-fracture structures. …”
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Development of cell lines with increased susceptibility to diverse adeno-associated viral vectors to enable in vitro potency assays by James Zengel, Emma S. Esterman, Anitha Ponnuswami, Nicholas R. Wall, Jan E. Carette

“…For the latter, we utilized guide RNAs targeting the conserved AAV2 inverted terminal repeat (ITR) sequence present in most AAV transfer vectors. Using this strategy, we engineered cell lines that showed marked increases in transduction by AAV vectors across a wide range of clinically relevant serotypes and containing cell-type-specific promoters. …”
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Deciphering TCOF1 mutations in Chinese Treacher Collins syndrome patients: insights into pathogenesis and transcriptional disruption by Zhuoyuan Jiang, Ke Mao, Bingqing Wang, Hao Zhu, Jiqiang Liu, Ruirui Lang, Baichuan Xiao, Hailin Shan, Qi Chen, Ying Li, Shouqin Zhao, Qingguo Zhang, Huisheng Liu, Yong-Biao Zhang

“…We identified 11 mutations predominantly localized to the central repeat domain (CRD) and the C-terminal domain (CTD, including the nuclear localization sequence) of TCOF1. The de novo frameshift mutation identified in the trio led to TCOF1 truncation, disrupting the central repeat domain crucial for binding transcriptional factors. …”
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Two complementing in vivo selection systems based on CCA-trimming exonucleases as a tool to monitor, select and evaluate enzymatic features of tRNA nucleotidyltransferases by Karolin Wellner, Josefine Gnauck, Dorian Bernier, Stephan H. Bernhart, Heike Betat, Mario Mörl

“…To fulfill this function, the enzyme contains a set of highly conserved motifs whose coordinated interplay is crucial for the sequence-specific CCA polymerization. In the human enzyme, alterations within these regions have been shown to lead to the manifestation of disease. …”
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A Survey of Cassini Images of Spokes in Saturn’s Rings: Unusual Spoke Types and Seasonal Trends by S. R. Callos, M. M. Hedman, D. P. Hamilton

“…In addition, we document seasonal variations in the overall spoke activity over the course of the Cassini mission using statistics derived from lit-side imaging sequences. These statistics demonstrate that while spokes can be detected over a wide range of solar elevation angles, spoke activity increases dramatically when the Sun is within 10° of the ring plane.…”
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Detection and identification of Naegleria species along with Naegleria fowleri in the tap water samples by Muhammad Aurongzeb, Syeda Zehratul Fatima, Syed Ikhlaq Hussain, Yasmeen Rashid, Tariq Aziz, Majid Alhomrani, Walaa F. Alsanie, Abdulhakeem S. Alamri

“…N. fowleri and other Naegleria species were detected in 11 out of 39 samples. Sequence analysis identified N. fowleri in tap water from the Golimar and Lyari areas of Karachi, while the other nine samples revealed different Naegleria species. …”
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Adsorption Characteristics of Phenol and Reactive Dyes from Aqueous Solution onto Ordered Mesoporous Carbons Prepared via a Template Synthesis Route by Taihong Shi, Yinghong Wen, Chunyu Ma, Shiguo Jia, Zhuochao Wang, Shujian Zou

“…The results showed that the order of the pore diameter in the mesoporous materials followed the sequence: CMK-3-100 > CMK-3-90 > CMK-3-80 > CMK-Na 2 SiO 3 -3-90. …”
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Bimodal Porous Scaffolds by Sequential Electrospinning of Poly(glycolic acid) with Sucrose Particles by B. Wulkersdorfer, K. K. Kao, V. G. Agopian, A. Ahn, J. C. Dunn, B. M. Wu, M. Stelzner

“…Poly(glycolic acid) (PGA) and a sucrose-ethanol suspension were electrospun in equal, alternating sequences at intervals of one, two, and ten minutes each. …”
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