Hepatic Iron Quantification on 3 Tesla (3 T) Magnetic Resonance (MR): Technical Challenges and Solutions by Muhammad Anwar, John Wood, Deepa Manwani, Benjamin Taragin, Suzette O. Oyeku, Qi Peng

“…We studied 22 sickle cell patients using multiecho fast gradient-echo sequence (MFGRE) 3 T MR and compared the results with serum ferritin and liver biopsy results. …”
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Serological and Molecular Characterization of the Hepatitis B Virus in Blood Donors in Maputo City, Mozambique by Olga Maquessene, Osvaldo Laurindo, Lúcia Chambal, Nalia Ismael, Nédio Mabunda

“…The median viral load of HBV-positive blood donors was 1288.5 IU/mL, and 43.8% had a viral load higher than 2000 IU/mL. Most of the sequenced samples (94.3%) belonged to subgenotype A1. …”
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Safety evaluation of the food enzyme containing endo‐polygalacturonase and β‐glucosidase from the non‐genetically modified Aspergillus tubingensis strain ARO by EFSA Panel on Food Enzymes (FEZ Panel), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Monika Sramkova, Henk Van Loveren, Laurence Vernis, Valentina Tokić, Ana Criado, Eleonora Marini, Laura Sanmartin Cabo, Yi Liu

“…A search for the homology of the amino acid sequence of the food enzymes to known allergens was made and four matches with food allergens and 22 matches with respiratory allergens were found. …”
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A next-generation Fab library platform directly yielding drug-like antibodies with high affinity, diversity, and developability by Fortunato Ferrara, Adeline Fanni, Andre A. R. Teixeira, Esteban Molina, Camila Leal-Lopes, Ashley DeAguero, Sara D’Angelo, M. Frank Erasmus, Laura Spector, Luis Antonio Rodriguez Carnero, Jianquan Li, Thomas J. Pohl, Nikolai Suslov, Klervi Desrumeaux, Conor McMahon, Sagar Kathuria, Andrew R. M. Bradbury

“…The platform design was based on the use of clinical antibodies as scaffolds into which replicated natural complementarity-determining regions purged of sequence liabilities were inserted, and the use of phage and yeast display to carry out antibody selection. …”
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Groping for Quantitative Digital 3-D Image Analysis: An Approach to Quantitative Fluorescence In Situ Hybridization in Thick Tissue Sections of Prostate Carcinoma by Karsten Rodenacker, Michaela Aubele, Peter Hutzler, P. S. Umesh Adiga

“…The gain or loss of certain base sequences in the desoxyribonucleic acid (DNA) can be estimated by counting the number of FISH signals per cell nucleus. …”
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Programmable Multiplexed Nucleic Acid Detection by Harnessing Specificity Defect of CRISPR‐Cas12a by Xin Guan, Rui Yang, Jiongyu Zhang, Jeong Moon, Chengyu Hou, Chong Guo, Lori Avery, Danielle Scarola, Daniel S. Roberts, Rocco LaSala, Changchun Liu

“…By harnessing the specificity defect of the CRISPR‐Cas12a enzyme, a dual‐mode detection strategy is designed, which includes approximate matching and precise querying of target sequences and develop a programmable multiplexed nucleic acid assay. …”
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Scavenger Receptor Class B, Type I, a CD36 Related Protein in Macrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sou... by Zhili Ding, Na Luo, Youqin Kong, Jingfen Li, Yixiang Zhang, Fang Cao, Jinyun Ye

“…The complete MnSR-BI coding sequence was 1545 bp, encoding 514 amino acid peptides. …”
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Nucleosome patterns in four plant pathogenic fungi with contrasted genome structures by Clairet, Colin, Lapalu, Nicolas, Simon, Adeline, Soyer, Jessica L., Viaud, Muriel, Zehraoui, Enric, Dalmais, Berengère, Fudal, Isabelle, Ponts, Nadia

“…Our findings support that nucleosome positioning and occupancies are subjected to evolution, in relation with underlying genome sequence modifications. Understanding genomic organization and its role in expression regulation is the next gear to understand complex cellular mechanisms and their evolution. …”
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Fast dynamical simulation combining harmonic-modal hybrid formulations with space separated representations by Claudia Germoso, Sebastian Rodriguez, Angelo Pasquale, Francisco Chinesta

“…These examples highlight the method's capability to handle high-resolution 3D problems efficiently, reducing it to a sequence of 1D or 2D problems, enabling significant computational time savings compared to the Finite Element Method (FEM).…”
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Study of Mitogenomes Provides Implications for the Phylogenetics and Evolution of the Infraorder Muscomorpha in Diptera by Huan Yuan, Wenbo Fu, Shulin He, Tingjing Li, Bin Chen

“…This study comparatively analyzed the characteristics of mitogenomes of 131 species that represent 18 superfamilies in Muscomorpha, in which mitogenomes of 16 species have been newly sequenced and annotated, demonstrating that their gene composition, order, AT bias, length variation, and codon usage are consistent with documented dipteran mitogenomes. …”
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A Bioinformatics Analysis Reveals a Group of MocR Bacterial Transcriptional Regulators Linked to a Family of Genes Coding for Membrane Proteins by Teresa Milano, Sebastiana Angelaccio, Angela Tramonti, Martino Luigi Di Salvo, Roberto Contestabile, Stefano Pascarella

“…An analysis of the multiple sequence alignments of their wHTH and AAT domains suggested the presence of specificity-determining positions (SDPs). …”
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Genomic insights into ecological adaptation of oaks revealed by phylogenomic analysis of multiple species by Tian-Rui Wang, Xin Ning, Si-Si Zheng, Yu Li, Zi-Jia Lu, Hong-Hu Meng, Bin-Jie Ge, Gregor Kozlowski, Meng-Xiao Yan, Yi-Gang Song

“…Here we used a newly sequenced genome of Quercus gilva, an evergreen oak species from East Asia, with 18 published Fagales genomes to determine how Fagaceae genomes have evolved, identify genomic footprints of ecological adaptability in oaks in general, as well as between evergreen and deciduous oaks. …”
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NO Reactions Over Ir-Based Catalysts in the Presence of O2 by Mingxin Guo, Rongshu Zhu, Minhua Dong, Feng Ouyang

“…When the loading is higher than 0.1%, the catalytic activity for NO oxidation is in order: Ir/ZSM-5>Ir/SiO2>Ir/γ -Al2O3, which is correlated with Ir dispersion on the surface of support materials and the catalytic activity for NO reduction is in sequence: Ir/γ -Al2O3>Ir/SiO2>Ir/ZSM-5, which is attributed to the adsorbed-dissociation of NO2. …”
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Optimizing transient gas network control for challenging real-world instances using MIP-based heuristics by Hennings, Felix, Hoppmann-Baum, Kai, Zittel, Janina

“…For the first task, we design multiple different heuristics based on concepts for general time-expanded optimization problems that find solutions by solving a sequence of sub-problems defined on reduced time horizons. …”
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Clinical Features and Genetic Background of the Periodic Fever Syndrome with Aphthous Stomatitis, Pharyngitis, and Adenitis: A Single Center Longitudinal Study of 81 Patients by Daša Perko, Maruša Debeljak, Nataša Toplak, Tadej Avčin

“…All four genes were PCR amplified and directly sequenced. Eighty-one patients fulfilled criteria for PFAPA syndrome, 50 (63%) boys and 31 (37%) girls, with mean age at disease onset of 2.1 ± 1.5 years. …”
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Predictive fatigue life modelling for aluminum alloys winder high temperature and shot peening interact by Allawi H. Alwin, Hatem Ksibi

“…Additionally, a mathematical model was developed, integrating the Stress – Number of cycles curve, loading sequence, temperature, and surface hardness from shot peening. …”
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Staging of Primary Abdominal Lymphomas: Comparison of Whole-Body MRI with Diffusion-Weighted Imaging and 18F-FDG-PET/CT by Alessandro Stecco, Francesco Buemi, Martina Quagliozzi, Mariangela Lombardi, Alberto Santagostino, Gian Mauro Sacchetti, Alessandro Carriero

“…The purpose of this study was to compare the accuracy of whole-body MRI with diffusion-weighted sequences (WB-DW-MRI) with that of 18F-FDG-PET/CT in the staging of patients with primary gastrointestinal lymphoma. …”
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Early Healing Events around Titanium Implant Devices with Different Surface Microtopography: A Pilot Study in an In Vivo Rabbit Model by Ester Orsini, Stefano Salgarello, Désirée Martini, Beatrice Bacchelli, Marilisa Quaranta, Luciano Pisoni, Emma Bellei, Monika Joechler, Vittoria Ottani

“…The insertion of titanium devices into the proximal tibia elicited a sequence of healing events. Newly formed bone proceeded through an early distance osteogenesis, common to both surfaces, and a delayed contact osteogenesis which seemed to follow different patterns at the two surfaces. …”
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Structural basis of Spliced Leader RNA recognition by the Trypanosoma brucei cap-binding complex by Harald Bernhard, Hana Petržílková, Barbora Popelářová, Kamil Ziemkiewicz, Karolina Bartosik, Marcin Warmiński, Laura Tengo, Henri Gröger, Luciano G. Dolce, Cameron D. Mackereth, Ronald Micura, Jacek Jemielity, Eva Kowalinski

“…During trans-splicing, a universal RNA sequence, the spliced leader RNA (SL RNA) mini-exon, is added to the 5’-end of each mRNA. …”
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Non-contrast enhanced MRI for efficiency evaluation of high-intensity focused ultrasound in adenomyosis ablation by Ma Si, Fajin Lv, Mingmei Tang, Yang Liu, Xueke Qiu, Chunmei Gong, Yan Hu, Yang Liu

“…The ablation areas on T2WI and DWI sequences were classified into different types: type I, relatively ill-defined rim or unrecognizable; subtype IIa, well-defined rim with hyperintensity; subtype IIb, well-defined rim with hypointensity. …”
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