Construction of a pathogenic microorganism detection method based on third-generation nanopore sequencing data by Guoqin Mai, Jiayi Chen, Min Zhang, Wanyue Zhang, Yuting Luo, Ying Dai

“…First, a comprehensive and penetrating reference database of nucleic acids from pathogenic microorganisms was constructed, and sequence alignment analysis was performed via nanopore sequencing of metagenomic data collected from 40 patients with lower respiratory tract infection symptoms. …”
Get full text

A Nonsynonymous Single-nucleotide Polymorphism in SLC24A5 Regulates Feather Pigment Deposition in Chinese Yellow Quail by Xiaohui Zhang, Fanghu Wu, Yanxia Qi, Yuanyuan Shang, Lingyun Fan, Yifei Wang

“…Potential single-nucleotide polymorphisms (SNPs) were screened using multiple sequence alignment. The screened nonsynonymous SNPs were genotyped across 265 Chinese yellow quails using the kompetitive allele-specific PCR method. …”
Get full text

Analysis of expression and transport activity of sugar transporter gene HpSWEET7 from red pitaya by ZHENG Qianming, YAN Shuang, XIE Pu, WANG Honglin, MA Yuhua

“…[Methods] HpSWEET7 gene was isolated from ‘Zihonglong’, and sequence alignment and phylogenetic relationship were analyzed. …”
Get full text

Surveillance of SARS-CoV-2 variants in Henan, China from 2023 to 2024 by Yun Song, Bicong Wu, Hongxia Ma, Yafei Li, Su Yan, Jingjing Pan, Haifeng Wang, Ying Ye, Xueyong Huang, Wanshen Guo

“…Genome sequence analysis was performed with CLC Genomics Workbench, and genotyping and sequence alignment were carried out using the Nextclade platform. …”
Get full text

Prevalence and Molecular Characterization of Cysticercus tenuicollis Isolated from Some Intermediate Host in Kurdistan-Iraq by Abdullah Ahmed Hama, Rostam Hama Zorab, Fatima Mohammed Ali, Awaz M. Salih, Amer Abdullah Hassan

“…The nucleotide sequence alignment of cytochrome oxidase subunit 1 (CO 1) gene revealed that the amplified DNA fragment is belong to Taenia hydatigena and Echinococcus granulosus and the nucleotide sequences of T. hydatigena deposited in GenBank under accession number (MH638348). …”
Get full text

Development of a 50K SNP array for whole-genome analysis and its application in the genetic localization of eggplant (Solanum melongena L.) fruit shape by Chuying Yu, Qihong Yang, Weiliu Li, Yaqin Jiang, Guiyun Gan, Liangyu Cai, Xinchun Li, Zhiqiang Li, Wenjia Li, Min Zou, Yang Yang, Yikui Wang

“…The BSA-seq data were located in the 78444173−84449348 interval on chromosome 3, with a size of 6 Mb, which was narrowed to 712.6 kb through fine mapping. Further sequence alignment and expression analysis revealed SmIQD14 as a candidate gene controlling eggplant fruit shape. …”
Get full text

Species classification of Tabanus (Diptera: Tabanidae) in Western Thailand: Integrating DNA barcoding and modern morphometrics by Tanasak Changbunjong, Thekhawet Weluwanarak, Sedthapong Laojun, Tanawat Chaiphongpachara

“…To enhance the reliability of species identification, DNA barcoding was employed to analyze 30 cytochrome c oxidase subunit 1 (cox1) gene sequences from 15 horse fly species, which were then compared with sequences in the GenBank and BOLD databases. Most cox1 sequences aligned with existing data, with similarity percentages ranging from 96% to 100%. …”
Get full text