A Protoplast System for CRISPR-Cas Ribonucleoprotein Delivery in <i>Pinus taeda</i> and <i>Abies fraseri</i> by Barbara M. Marques, Daniel B. Sulis, Bethany Suarez, Chenmin Yang, Carlos Cofre-Vega, Robert D. Thomas, Justin G. A. Whitehill, Ross W. Whetten, Rodolphe Barrangou, Jack P. Wang

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Current state and prospects of protoplast technology and potato somatic hybridization (review) by O. B. Polivanova, A. S. Egorova, A. B. Sivolapova, S. V. Goryunova

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Transformation of CryK13V gene into protoplasts of Trichoderma viride by LIU Shi-wang, GUO Ze-jian, JIANG Dong-hua, WANG Zheng-yi

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The isolation and regeneration of protoplasts from the mycorrhizal fungi Lactarius piperatus and Suillus variegatus by Jana Zel, Marina Dermastia, Nada Gogala

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Construction of biological control strain of Trichoderma viride and study of their ability to induce plant disease resistance by LIU Shi-wang, GUO Ze-jian

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Germplasm cryopreservation of Dendrobium candidum by vitrification by CHEN Yong, WANG Jun-hui, HUANG Chun-nong

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Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in <i>Cordyceps cicadae</i> by Haikun Qi, Haihua Ruan, Tao Wu, Hongyang Zhang, Rui Dong, Yanjun Jiang

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The Q‐Warg Pipeline: A Robust and Versatile Workflow for Quantitative Analysis of Protoplast Culture Conditions by Léa Bogdziewiez, Rik Froeling, Patricia Schöppl, Jeanne Juquel, Ioanna Antoniadi, Vladimìr Skalický, Ambroise Mathey, Jacques Fattaccioli, Joris Sprakel, Stéphane Verger

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Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage by Agnieszka Kiełkowska, Agnieszka Brąszewska

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The first single-cell sequencing of Plasmodiophora brassicae reveals genetic diversity and clonal dynamics by Afsaneh Sedaghatkish, Meik Kunz, Bruce D. Gossen, Mary Ruth McDonald

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Microfilament cytoskeletons in BY-2 protoplasts and broad bean mesophyll protoplasts and the effect of latrunculin B on their distribution by YE Lu-fei, LIU Cheng-ke, MENG Chun-mei, HONG Jian

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Phenotype microarray-based assessment of metabolic variability in plant protoplasts by Alice Checcucci, Francesca Decorosi, Giulia Alfreducci, Roberto Natale, Agnese Bellabarba, Stefano Biricolti, Donatella Paffetti, Alessio Mengoni, Carlo Viti

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Mutagenesis of Ganoderma lucidum protoplasts for high rate of biomass production and Se⁺⁴-rich isolates by LIU Shi-wang, GUO Ze-jian, KAN Yun-chao, LI Nan-yi, LI Jin

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DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection by Ryan Creeth, Andrew Thompson, Zoltan Kevei

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Unlocking male sterility in horticultural crops through gene editing technology for precision breeding applications: presentation of a case study in tomato by Silvia Farinati, Adriana Fernanda Soria Garcia, Samela Draga, Alessandro Vannozzi, Fabio Palumbo, Francesco Scariolo, Giovanni Gabelli, Gianni Barcaccia

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Protoplast Isolation in Lupin Lupinus spp by Necmi Beşer, Andrew Wetten

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Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Glycine max [L.] Merr.) by Kihwan Kim, Kihwan Kim, Junseop Shin, Jeong-Dong Lee, Jeong-Dong Lee, Jeong-Dong Lee, Won-Chan Kim, Won-Chan Kim

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Development of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L. by Katarzyna Stelmach-Wityk, Kamil Szymonik, Ewa Grzebelus, Agnieszka Kiełkowska

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Optimization of Protoplast Preparation Conditions in <i>Lyophyllum decastes</i> and Transcriptomic Analysis Throughout the Process by Xiaobin Li, Ying Qin, Yufei Kong, Samantha Chandranath Karunarathna, Yunjiang Liang, Jize Xu

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Technology and optimization of preparation and regeneration of protoplasts from rice sheath blight fungus Rhizoctonia solani by KONG Dandan, QUE Yawei, YAN Xia, LI Ya, CHEN Weiliang, WANG Zhengyi

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