Executioner caspases degrade essential mediators of pathogen-host interactions to inhibit growth of intracellular Listeria monocytogenes by Marilyne Lavergne, Raffael Schaerer, Sara De Grandis, Safaa Bouheraoua, Oluwadamilola Adenuga, Tanja Muralt, Tiffany Schaerer, Léa Chèvre, Alessandro Failla, Patricia Matthey, Michael Stumpe, Dieter Kressler, Pierre-Yves Mantel, Michael Walch

“…Here, by means of chemical and genetical modifications, we show that the executioner caspases-3 and -7 efficiently inhibit growth of intracellular Listeria monocytogenes in host cells. Comprehensive proteomics revealed multiple caspase-3 substrates in the Listeria secretome, including LLO, Iap and various other proteins crucially involved in pathogen-host interactions. …”
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Enantioselective OTUD7B fragment discovery through chemoproteomics screening and high-throughput optimisation by Aini Vuorinen, Cassandra R. Kennedy, Katherine A. McPhie, William McCarthy, Jonathan Pettinger, J. Mark Skehel, David House, Jacob T. Bush, Katrin Rittinger

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Widespread release of translational repression across Plasmodium's host-to-vector transmission event. by Kelly T Rios, James P McGee, Aswathy Sebastian, Sanjaya Aththawala Gedara, Robert L Moritz, Marina Feric, Sabrina Absalon, Kristian E Swearingen, Scott E Lindner

“…Therefore, we have conducted the first quantitative, comparative transcriptomics and DIA-MS proteomics of Plasmodium parasites across the host-to-vector transmission event to document the global release of translational repression. …”
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Protocol for generating protein profiles and distance-based network analysis of murine tissue slices by Luisa Schmidt, Philipp Antczak, Marcus Krüger

“…Summary: We introduce a protocol for spatial proteomics using thin cryotome sections of mouse skeletal muscle tissue. …”
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The Role of Organelle Stresses in Diabetes Mellitus and Obesity: Implication for Treatment by Yi-Cheng Chang, Siow-Wey Hee, Meng-Lun Hsieh, Yung-Ming Jeng, Lee-Ming Chuang

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The Mycobacterium ulcerans toxin mycolactone causes destructive Sec61-dependent loss of the endothelial glycocalyx and vessel basement membrane to drive skin necrosis by Louise Tzung-Harn Hsieh, Belinda S Hall, Jane Newcombe, Tom A Mendum, Sonia Santana Varela, Yagnesh Umrania, Michael J Deery, Wei Q Shi, Josué Diaz-Delgado, Francisco J Salguero, Rachel E Simmonds

“…Unbiased quantitative proteomics identified a profound effect on proteoglycans, driven by rapid loss of type II transmembrane proteins of the Golgi, including enzymes required for glycosaminoglycan (GAG) synthesis, combined with a reduction in the core proteins themselves. …”
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Inhibition of tumour necrosis factor alpha by Etanercept attenuates Shiga toxin-induced brain pathology by Robin Christ, Devon Siemes, Shuo Zhao, Lars Widera, Philippa Spangenberg, Julia Lill, Stephanie Thiebes, Jenny Bottek, Lars Borgards, Andreia G. Pinho, Nuno A. Silva, Susana Monteiro, Selina K. Jorch, Matthias Gunzer, Bente Siebels, Hannah Voss, Hartmut Schlüter, Olga Shevchuk, Jianxu Chen, Daniel R. Engel

“…In this study, we investigated the role of tumour necrosis factor alpha (TNF-α) for microglial activation and brain pathology using a preclinical mouse model of EHEC infection. LC–MS/MS proteomics of mice injected with a combination of Shiga toxin (Stx) and lipopolysaccharide (LPS) revealed extensive alterations of the brain proteome, in particular enrichment of pathways involved in complement activation and coagulation cascades. …”
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Toward measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime by Debjit Roy, Xavier Michalet, Evan W. Miller, Kiran Bharadwaj, Shimon Weiss

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High-Density Lipoprotein Reduction Differentially Modulates to Classical and Nonclassical Monocyte Subpopulations in Metabolic Syndrome Patients and in LPS-Stimulated Primary Human... by Johanna L. Grün, Aaron N. Manjarrez-Reyna, Angélica Y. Gómez-Arauz, Sonia Leon-Cabrera, Felix Rückert, José M. Fragoso, Nallely Bueno-Hernández, Sergio Islas-Andrade, Guillermo Meléndez-Mier, Galileo Escobedo

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Nonclinical immunogenicity risk assessment for knobs-into-holes bispecific IgG1 antibodies by Wen-Ting K. Tsai, Yinyin Li, Zhaojun Yin, Peter Tran, Qui Phung, Zhenru Zhou, Kun Peng, Dan Qin, Sien Tam, Christoph Spiess, Jochen Brumm, Manda Wong, Zhengmao Ye, Patrick Wu, Sivan Cohen, Paul J. Carter

“…Assay methods used include dendritic cell internalization, T cell proliferation, and T cell epitope identification by in silico prediction and MHC-associated peptide proteomics. Data from each method were considered independently and then together for an overall integrated immunogenicity risk assessment. …”
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Interaction Between Malat1 and miR-499-5p Regulates Meis1 Expression and Function with a Net Impact on Cell Proliferation by Salma A. Fahim, Manon Ragheb, Ibrahim Hassan Fayed, Aya Osama, Ahmed Karam, Sameh Magdeldin, Rana Metwale, Mohamed Dief Allah Abdalmoneam Elsayed, Ahmed Abdellatif, Hesham A. Sadek, Shereen Ahmed El Sobky, Nada El-Ekiaby, Injie Omar Fawzy, Ahmed Ihab Abdelaziz

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Discovery of a sushi domain-containing protein 2-positive phenotype in circulating tumor cells of metastatic breast cancer patients by Kai Bartkowiak, Parinaz Mossahebi Mohammadi, Paula Nissen, Stefan Werner, David Agorku, Antje Andreas, Maria Geffken, Sven Peine, Karl Verpoort, Thomas M. Deutsch, Laura L. Michel, Andreas Schneeweiss, Verena Thewes, Andreas Trumpp, Olaf Hardt, Volkmar Müller, Sabine Riethdorf, Hartmut Schlüter, Klaus Pantel

“…After a LC-MC/MS based proteomics analysis of CTC-ITB-01, we found extraordinary high levels of the poorly characterized protein SUSD2 (sushi domain-containing protein 2) in CTC-ITB-01. …”
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Contribution of hypoxia-inducible factor 1alpha to pathogenesis of sarcomeric hypertrophic cardiomyopathy by Sarala Raj Murthi, Andreas Petry, Bachuki Shashikadze, Jan B. Stöckl, Manuel Schmid, Gianluca Santamaria, Karin Klingel, Damir Kračun, Xinpei Chen, Sabine Bauer, Joachim P. Schmitt, Florian Flenkenthaler, Josh Gorham, Christopher N. Toepfer, David Potěšil, Pavel Hruška, Zbyněk Zdráhal, Zsuzsanna Mayer, Mathieu Klop, Luisa Lehmann, Yishi Qin, Laura Papanakli, Nadine Spielmann, Alessandra Moretti, Thomas Fröhlich, Peter Ewert, Stefan Holdenrieder, Jonathan G. Seidman, Christine E. Seidman, Agnes Görlach, Cordula M. Wolf

“…Cardiomyocyte-specific abolishment of Hif-1a blunted the disease phenotype, as evidenced by decreased left ventricular wall thickness, reduced myocardial fibrosis, disordered SRX/DRX state and ROS production. cHif1aKO induced normalization of pro-hypertrophic and pro-fibrotic left ventricular remodeling signaling evidenced on whole transcriptome and proteomics analysis in α-MHC719/+ mice. Proteomics of serum samples from patients with early onset HCM revealed significant modulation of HIF. …”
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Usefulness of C-Reactive Protein as a Marker for Prediction of Future Coronary Events in the Asian Indian Population: Indian Atherosclerosis Research Study by Veena S. Rao, Natesha B. Kadarinarasimhiah, Shibu John, Sridhara Hebbagodi, Jayashree Shanker, Vijay V. Kakkar

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Cryptic transmission of a SARS-CoV-2 variant detected by wastewater surveillance in Panama by Melissa Gaitán, Yamitzel Zaldivar, Michelle Hernandez, Jessica Góndola, Oris Chavarría, Brechla Moreno, Danilo Franco, Rodrigo DeAntonio, Santiago Mirazo, Florencia Cancela, Maria Eugenia Barnett, Alexander A. Martinez, Juan Miguel Pascale, Sandra López-Vergès

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Neuron-Specific Glycine Metabolism Links Transfer RNA Epitranscriptomic Regulation to Complex Behaviors by Jennifer Blaze, Viviana Dolores Evans, Jessica Abigail Feria Pliego, Petr Unichenko, Behnam Javidfar, Soeren Heissel, Hanan Alwaseem, Zachary Pennington, Denise Cai, Henrik Molina, Christian Henneberger, Schahram Akbarian

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Acquired vulnerability against EGF receptor inhibition in gastric cancer promoted by class I histone deacetylase inhibitor entinostat by Tamara Zenz, Robert Jenke, Denys Oliinyk, Sandra Noske, René Thieme, Tim Kahl, Ines Gockel, Florian Meier-Rosar, Achim Aigner, Thomas RH Büch

“…Based on these results, a combined entinostat plus EGFR inhibitor erlotinib treatment in vitro and in vivo was studied. Results: Proteomics analyses in gastric cancer cells treated with entinostat revealed a marked upregulation of EGFR in the majority of cell lines and an even more robust induction of the EGFR ligand AREG. …”
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The developmental lipidome of Nippostrongylus brasiliensis by Tao Wang, Michael G. Leeming, Nicholas A. Williamson, Tiffany Bouchery, Rory Doolan, Graham Le Gros, Gavin E. Reid, Nicola L. Harris, Robin B. Gasser

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Identification of KIFC1 as an independent prognostic marker in renal clear cell carcinoma correlates with tumor proliferation and immune infiltration by Bin Du, Jia Wang, Jinping Zheng, Jing Huo, Pu Wang

“…We analyzed the prognostic value, associated signaling pathways, and regulatory mechanisms of KIFC1 in ccRCC through bioinformatics and proteomics. Concretely, both mRNA and protein expression levels of KIFC1 were dramatically upregulated. …”
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Corrigendum to “Depletion of ß1,6-N-acetylglucosaminyltransferase reduces E-selectin binding capacity and migratory potential of human gastrointestinal adenocarcinoma cells” [Neopl... by Lisa Staffeldt, Hanna Maar, Julia Beimdiek, Samuel Chambers, Kristoffer Riecken, Mark von Itzstein, Falk FR Buettner, Arun Everest-Dass, Tobias Lange

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