Nonclinical immunogenicity risk assessment for knobs-into-holes bispecific IgG1 antibodies by Wen-Ting K. Tsai, Yinyin Li, Zhaojun Yin, Peter Tran, Qui Phung, Zhenru Zhou, Kun Peng, Dan Qin, Sien Tam, Christoph Spiess, Jochen Brumm, Manda Wong, Zhengmao Ye, Patrick Wu, Sivan Cohen, Paul J. Carter

“…Assay methods used include dendritic cell internalization, T cell proliferation, and T cell epitope identification by in silico prediction and MHC-associated peptide proteomics. Data from each method were considered independently and then together for an overall integrated immunogenicity risk assessment. …”
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Interaction Between Malat1 and miR-499-5p Regulates Meis1 Expression and Function with a Net Impact on Cell Proliferation by Salma A. Fahim, Manon Ragheb, Ibrahim Hassan Fayed, Aya Osama, Ahmed Karam, Sameh Magdeldin, Rana Metwale, Mohamed Dief Allah Abdalmoneam Elsayed, Ahmed Abdellatif, Hesham A. Sadek, Shereen Ahmed El Sobky, Nada El-Ekiaby, Injie Omar Fawzy, Ahmed Ihab Abdelaziz

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Discovery of a sushi domain-containing protein 2-positive phenotype in circulating tumor cells of metastatic breast cancer patients by Kai Bartkowiak, Parinaz Mossahebi Mohammadi, Paula Nissen, Stefan Werner, David Agorku, Antje Andreas, Maria Geffken, Sven Peine, Karl Verpoort, Thomas M. Deutsch, Laura L. Michel, Andreas Schneeweiss, Verena Thewes, Andreas Trumpp, Olaf Hardt, Volkmar Müller, Sabine Riethdorf, Hartmut Schlüter, Klaus Pantel

“…After a LC-MC/MS based proteomics analysis of CTC-ITB-01, we found extraordinary high levels of the poorly characterized protein SUSD2 (sushi domain-containing protein 2) in CTC-ITB-01. …”
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Contribution of hypoxia-inducible factor 1alpha to pathogenesis of sarcomeric hypertrophic cardiomyopathy by Sarala Raj Murthi, Andreas Petry, Bachuki Shashikadze, Jan B. Stöckl, Manuel Schmid, Gianluca Santamaria, Karin Klingel, Damir Kračun, Xinpei Chen, Sabine Bauer, Joachim P. Schmitt, Florian Flenkenthaler, Josh Gorham, Christopher N. Toepfer, David Potěšil, Pavel Hruška, Zbyněk Zdráhal, Zsuzsanna Mayer, Mathieu Klop, Luisa Lehmann, Yishi Qin, Laura Papanakli, Nadine Spielmann, Alessandra Moretti, Thomas Fröhlich, Peter Ewert, Stefan Holdenrieder, Jonathan G. Seidman, Christine E. Seidman, Agnes Görlach, Cordula M. Wolf

“…Cardiomyocyte-specific abolishment of Hif-1a blunted the disease phenotype, as evidenced by decreased left ventricular wall thickness, reduced myocardial fibrosis, disordered SRX/DRX state and ROS production. cHif1aKO induced normalization of pro-hypertrophic and pro-fibrotic left ventricular remodeling signaling evidenced on whole transcriptome and proteomics analysis in α-MHC719/+ mice. Proteomics of serum samples from patients with early onset HCM revealed significant modulation of HIF. …”
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Inflammation-Related Mechanisms in Chronic Kidney Disease Prediction, Progression, and Outcome by Simona Mihai, Elena Codrici, Ionela Daniela Popescu, Ana-Maria Enciu, Lucian Albulescu, Laura Georgiana Necula, Cristina Mambet, Gabriela Anton, Cristiana Tanase

“…Recent omics advances could provide novel insights in deciphering the disease pathophysiology; thus, identification of circulating biomarker panels using state-of-the-art proteomic technologies could improve CKD early diagnosis, monitoring, and prognostics. …”
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Characterization of the procoagulant phenotype of amniotic fluid across gestation in rhesus macaques and humans by Chih Jen Yang, Lyndsey E. Shorey-Kendrick, Cristina Puy, Ashley E. Benson, Phillip A. Wilmarth, Ashok P. Reddy, Keith D. Zientek, Kilsun Kim, Adam Crosland, Chaevien S. Clendinen, Lisa M. Bramer, Olivia L. Hagen, Helen H. Vu, Joseph E. Aslan, Owen J.T. McCarty, Joseph J. Shatzel, Brian P. Scottoline, Jamie O. Lo

“…Methods: We analyzed the proteomes, lipidomes, and procoagulant properties of AF obtained by amniocentesis from rhesus macaque and human pregnancies at gestational age-matched time points. …”
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Impact of High Glucose on Bone Collagenous Matrix Composition, Structure, and Organization: An Integrative Analysis Using an Ex Vivo Model by Rita Araújo, Ricardo N. M. J. Páscoa, Raquel Bernardino, Pedro S. Gomes

“…A comprehensive approach integrating histological evaluation, histomorphometry, ATR-FTIR spectroscopy, and proteomics was adopted to unravel structural, biochemical, and molecular changes in the ECM. …”
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Functional heterogeneity of mesenchymal stem cells and their therapeutic potential in the K18-hACE2 mouse model of SARS-CoV-2 infection by Kátia Nunes da Silva, Fernanda Martins Marim, Gisele Vieira Rocha, Zaquer Suzana Munhoz Costa-Ferro, Luciana Souza de Aragão França, Carolina Kymie Vasques Nonaka, Bruno Diaz Paredes, Erik Aranha Rossi, Erick Correia Loiola, Corynne Stephanie Ahouefa Adanho, Rachel Santana Cunha, Mayck Medeiros Amaral da Silva, Fernanda Ferreira Cruz, Vivian Vasconcelos Costa, Dalila Lucíola Zanette, Clarissa Araújo Gurgel Rocha, Renato Santana Aguiar, Patricia Rieken Macedo Rocco, Bruno Solano de Freitas Souza

“…The cells were assessed for their proliferative capacity, immunophenotype, trilineage differentiation, proteomic profile, and in vitro immunomodulatory potential by co-culture with activated lymphocytes. …”
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Sex differences in epigenetic ageing for older people living with HIVResearch in context by Carrie D. Johnston, Alina P.S. Pang, Eugenia L. Siegler, Charlene Thomas, Chelsie O. Burchett, Mia Crowley, Rochelle O'Brien, Lishomwa C. Ndhlovu, Marshall J. Glesby, Michael J. Corley

“…We profiled plasma biomarkers with Olink high-throughput proteomics to test associations with epigenetic age acceleration. …”
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Propionate metabolism in Desulfurella acetivorans by Eugenio Pettinato, Thomas M. Steiner, Eric A. Cassens, Thomas Geisberger, Christian Seitz, Simone König, Wolfgang Eisenreich, Ivan A. Berg

“…However, activities of the methylcitrate cycle enzymes could not be detected in extracts of propionate-grown cells, and experiments with [U-13C3]propionate and comparative proteomic analysis of acetate- and propionate-grown cells suggested that the methylcitrate cycle is not active during propionate growth. …”
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Usefulness of C-Reactive Protein as a Marker for Prediction of Future Coronary Events in the Asian Indian Population: Indian Atherosclerosis Research Study by Veena S. Rao, Natesha B. Kadarinarasimhiah, Shibu John, Sridhara Hebbagodi, Jayashree Shanker, Vijay V. Kakkar

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Cryptic transmission of a SARS-CoV-2 variant detected by wastewater surveillance in Panama by Melissa Gaitán, Yamitzel Zaldivar, Michelle Hernandez, Jessica Góndola, Oris Chavarría, Brechla Moreno, Danilo Franco, Rodrigo DeAntonio, Santiago Mirazo, Florencia Cancela, Maria Eugenia Barnett, Alexander A. Martinez, Juan Miguel Pascale, Sandra López-Vergès

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Neuron-Specific Glycine Metabolism Links Transfer RNA Epitranscriptomic Regulation to Complex Behaviors by Jennifer Blaze, Viviana Dolores Evans, Jessica Abigail Feria Pliego, Petr Unichenko, Behnam Javidfar, Soeren Heissel, Hanan Alwaseem, Zachary Pennington, Denise Cai, Henrik Molina, Christian Henneberger, Schahram Akbarian

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The cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum... by Magdalena Zaranek, Artur Pinski, Bozena Skupien-Rabian, Urszula Jankowska, Kamila Godel-Jedrychowska, Katarzyna Sala-Cholewa, Katarzyna Nowak, Ewa Kurczyńska, Ewa Grzebelus, Alexander Betekhtin

“…To gain deeper insights into the stages of cell development from protoplasts—such as cell division, cell colony formation, and microcalli development—we focused on analyzing proteomes, cell wall composition, and changes in the expression profiles of selected genes in Fagopyrum protoplast cultures. …”
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Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection by Niharika Bala, Ramish H. Rafay, Sarah C. Glover, Abdel A. Alli

“…Urinary extracellular vesicles (uEVs) were isolated from urine samples from the two groups and used for proteomic analysis and subsequent pathway analyses. …”
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EMC2 suppresses ferroptosis via regulating TFRC in nasopharyngeal carcinoma by Xianghui Chen, Xiaoyan Wang, Yuxia Zou, Yan Wang, Tingting Duan, Zijie Zhou, Yi Huang, Qing Ye

“…Method: We conducted quantitative proteomics to detect dysregulated proteins in NPC tissues. …”
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Anaerobic metabolism promotes breast cancer survival via Histone-3 Lysine-18 lactylation mediating PPARD axis by Ying Xu, Weiwei Meng, Yingqi Dai, Lin Xu, Ning Ding, Jinqing Zhang, Xuewei Zhuang

“…Additionally, we confirmed that HDAC2 and HDAC3 act as “erasers” for H3 lysine lactylation. During the proteomics analysis, AKT-phosphorylation in the aerobic respiration inhibitor group exhibited an apparent disparity and activity. …”
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Acquired vulnerability against EGF receptor inhibition in gastric cancer promoted by class I histone deacetylase inhibitor entinostat by Tamara Zenz, Robert Jenke, Denys Oliinyk, Sandra Noske, René Thieme, Tim Kahl, Ines Gockel, Florian Meier-Rosar, Achim Aigner, Thomas RH Büch

“…Based on these results, a combined entinostat plus EGFR inhibitor erlotinib treatment in vitro and in vivo was studied. Results: Proteomics analyses in gastric cancer cells treated with entinostat revealed a marked upregulation of EGFR in the majority of cell lines and an even more robust induction of the EGFR ligand AREG. …”
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The developmental lipidome of Nippostrongylus brasiliensis by Tao Wang, Michael G. Leeming, Nicholas A. Williamson, Tiffany Bouchery, Rory Doolan, Graham Le Gros, Gavin E. Reid, Nicola L. Harris, Robin B. Gasser

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Properties of Actinobacillus pleuropneumoniae isolates by V. А. Evgrafova, О. V. Pruntova, N. B. Shadrova, А. М. Timina

“…Results of tests of six Actinobacillus pleuropneumoniae isolates recovered from the diseased pigs kept in animal holdings located in the Russian Federation for their biological properties (biochemical, proteomic, antigenic and pathogenic ones) are presented in the paper. …”
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