FDFT1 inhibits macrophage M1 polarization and promotes colorectal cancer progression by GAO Yuan, HUANG Yulan, ZHAO Kun

“…RT-qPCR was applied to detect the mRNA expression of FDFT1, and Western blotting was conducted to measure the protein level of FDFT1. C57BL/6 mice were subjected randomly to construct a subcutaneous tumor-bearing model and a model of intraperitoneal metastatic tumor (n=5) respectively. …”
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Quantitative N-glycoproteomic analysis reveals glycosylation signatures of plasma immunoglobulin G in systemic sclerosis by Lu Cheng, Lu Cheng, Yanhong Li, Yu Zhou, Yingying Ling, Tong Wu, Zongan Liang, Yinlan Wu, Chunyu Tan, Yi Liu, Yong Zhang

“…These changes in the levels of intact N-glycopeptides are associated with various aspects of SSc, including diffuse SSc (dSSc), interstitial lung disease (ILD), disease progression, cardiovascular involvement and C-reactive protein in the peripheral blood. In summary, this study offers a detailed overview of the intact N-glycopeptide profile in the peripheral blood of patients with SSc, providing valuable insights that could propel further research into SSc.…”
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Combination gemcitabine and PD-L1xCD3 bispecific T cell engager (BiTE) enhances T lymphocyte cytotoxicity against cholangiocarcinoma cells by Methi Wathikthinnakon, Piriya Luangwattananun, Nunghathai Sawasdee, Chutipa Chiawpanit, Vannajan Sanghiran Lee, Piyarat Nimmanpipug, Yingmanee Tragoolpua, Siriphorn Rotarayanont, Thanich Sangsuwannukul, Nattaporn Phanthaphol, Yupanun Wutti-in, Chalermchai Somboonpatarakun, Thaweesak Chieochansin, Mutita Junking, Jatuporn Sujjitjoon, Pa-thai Yenchitsomanus, Aussara Panya

“…However, gemcitabine treatment of CCA cells upregulates the expression of an immune checkpoint protein (programmed death-ligand 1 [PD-L1]) that consequently inhibits the cytotoxicity of T lymphocytes. …”
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Ibrutinib disrupts blood-tumor barrier integrity and prolongs survival in rodent glioma model by Sanghee Lim, Minhye Kwak, Jeonghan Kang, Melissa Cesaire, Kayen Tang, Robert W. Robey, William J. E. Frye, Baktiar Karim, Donna Butcher, Martin J. Lizak, Mahalia Dalmage, Brandon Foster, Nicholas Nuechterlein, Charles Eberhart, Patrick J. Cimino, Michael M. Gottesman, Sadhana Jackson

“…Expression of tight junction gene and protein expression was decreased maximally 4 h after administration, along with inhibition of efflux transporter, ABCB1, activity. …”
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Meteorin-like alleviates hepatic steatosis by regulating hepatic triglyceride secretion and fatty acid oxidation by Lingyu Song, Yali Huang, Lu Liu, Xuebing Chang, Laying Hu, Guifang Wang, Lifen Xu, Tian Zhang, Yuanyuan Wang, Ying Xiao, Hong Yang, Suye Ran, Qing Shi, Tuanlao Wang, Mingjun Shi, Yuxia Zhou, Bing Guo

“…Mechanistically, Metrnl exerted dual effects by promoting triglyceride (TG) transportation by the phosphatidylinositol 3-kinase (PI3K)/Akt/Sp1/cytidylyltransferase α (CCTα) axis, thereby increasing the biosynthesis of phosphatidylcholine (PC) to facilitate TG secretion from the liver while facilitating AMP-activated protein kinase (AMPK)-dependent fatty acid oxidation (FAO). …”
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Genetic interaction between oxidative stress and body mass index in a Spanish population by Francisco Lara-Hernández, Rebeca Melero, María Elena Quiroz-Rodríguez, Celeste Moya-Valera, Mariana de Jesús Gallardo-Espinoza, Luis Álvarez, Ingrid Lizeth Valarezo-Torres, Laisa Briongos-Figuero, Jessica Abadía-Otero, Francisco Javier Mena-Martin, Guillermo Saez, Josep Redon, Juan-Carlos Martín-Escudero, Ana-Bárbara García-García, Guillermo Ayala, Felipe Javier Chaves

“…Oxidative stress has the potential to modulate genetic activity at various levels, including gene transcription and protein function regulation. Nevertheless, the interplay between genetic variants and oxidative stress in relation to BMI remains to be elucidated. …”
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CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris by Kaidi Chen, Gulikezi Maimaitirexiati, Qiannan Zhang, Yi Li, Xiangjian Liu, Hongting Tang, Xiang Gao, Bo Wang, Tao Yu, Shuyuan Guo

“…The important methylotrophic yeast Pichia pastoris has been utilized for the production of a variety of heterologous recombinant proteins and has great potential for use in the production of value-added compounds using methanol as a substrate. …”
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Perfluorooctane sulfonate (PFOS) induced bone loss by inhibiting FoxO1-mediated defense against oxidative stress in osteoblast by Liming Xue, Jiale Xu, Ping Xiao, Yiping Jiang, Yuanjie Lin, Chao Feng, Yu’e Jin, Zhijun Zhou, Guoquan Wang, Dasheng Lu

“…Vation of important genes and protein expression was accomplished using RT-PCR and Western blot methods, respectively. …”
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Reduction of intestinal RIPK1 ameliorates HFD-induced metabolic disorders in female mice by Ye Yuan, Xiaomin Hu, Chunguang Guo, Yihua Xu, Shihan Li, Wei Wen, Xinli Hu, Fanxin Zeng, Weiyi Cui, Wenli Chen, Xueting Sun, Ning Hou, Jue Wang, Rui-Ping Xiao, Xiuqin Zhang

“…In this study, using receptor-interacting protein kinase 1 (Ripk1) intestine-specific heterozygous knockout mice (Ripk1IEC+/−) and high-fat diet (HFD)-feeding mouse model, we report that HFD-induced shift in the transcriptional profile of the ileum toward that of the jejunum, characterized by increased expression of jejunal feature genes in the ileum, are attenuated in Ripk1IEC+/− female mice, but not in males. …”
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Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions by Batyrbek I. Aslanov, Daniil V. Azarov, Maria A. Makarova, Elizaveta G. Marysheva, Lyudmila A. Kraeva, Aleksey S. Mokhov, Ekaterina A. Lebedeva, Nikita E. Goncharov, Natalya V. Lebedeva, Dmitry A. Starikov, Victoria V. Kolodzhieva, Dmitry E. Polev, Artemy E. Goncharov

“…Among the pathogenicity factors identified were haemolysins A, E, F, siderophores, including the yersiniabactin gene cluster, a number of adhesion, colonization and invasion factors, as well as the thermostable enterotoxin EAST-1 and genes that characterize enteroaggregative strains of E. coli (the virulence regulator gene eilA and enteroaggregative protein (air)). One of the Arctic strains (33-1) had determinants of antibiotic resistance, in particular the extended-spectrum beta-lactamase gene TEM-1b and the Tn1721 transposon, including tetracycline resistance genes (tetA-TetR), were detected in its genome. …”
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A novel high-throughput screen identifies phenazine-1-carboxylic acid as an inhibitor of African swine fever virus replication in primary porcine alveolar macrophages by Jing Lan, Rui Luo, Di Liu, Changxing Qi, Xin Song, Zhanhao Lu, Ruojia Huang, Yuying Yang, Yuan Sun, Yonghui Zhang, Tao Wang, Hua-Ji Qiu

“…The enhanced green fluorescent protein (EGFP)- and Gaussia luciferase (Gluc)-encoding genes were incorporated downstream of the ASFV MGF300-4L gene without disrupting viral genes. …”
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Visceral adipose tissue area and proportion provide distinct reflections of cardiometabolic outcomes in weight loss; pooled analysis of MRI-assessed CENTRAL and DIRECT PLUS dietary... by Hadar Klein, Hila Zelicha, Anat Yaskolka Meir, Ehud Rinott, Gal Tsaban, Alon Kaplan, Yoash Chassidim, Yftach Gepner, Matthias Blüher, Uta Ceglarek, Berend Isermann, Michael Stumvoll, Ilan Shelef, Lu Qi, Jun Li, Frank B. Hu, Meir J. Stampfer, Iris Shai

“…Conversely, higher VATcm2 was associated with elevated high-sensitivity C-reactive protein (hsCRP), while VAT% was not. After 18 months of lifestyle intervention, both VATcm2 and VAT% loss were significantly associated with decreased triglycerides, HbA1c, ferritin, and liver enzymes, and increased HDL-c levels beyond weight loss (FDR < 0.05). …”
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Golgi-restored vesicular replenishment retards bone aging and empowers aging bone regeneration by Peisheng Liu, Hao Guo, Xiaoyao Huang, Anqi Liu, Ting Zhu, Chenxi Zheng, Fei Fu, Kaichao Zhang, Shijie Li, Xinyan Luo, Jiongyi Tian, Yan Jin, Kun Xuan, Bingdong Sui

“…Interestingly, replenishment of cell aggregates-derived extracellular vesicles (CA-EVs) rescues Golgi dysfunction and empowers senescent MSCs through the Golgi regulatory protein Syntaxin 5. Importantly, in vivo administration of CA-EVs significantly enhanced the bone defect repair rate and improved bone mass in aging mice, suggesting their therapeutic value for treating age-related osteoporosis and promoting bone regeneration. …”
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Biogenic silver nanoparticles produced by Trichoderma reesei inhibit SARS-CoV-2 infection, reduce lung viral load and ameliorate acute pulmonary inflammation by Marcus V.M.V. Amaral, Cláudia B. Carraro, Amanda C.C. Antoniêto, Mariana N. Costa, Thais F.C. Fraga-Silva, Ualter G. Cipriano, Rodrigo P.F. Abuná, Tamara S. Rodrigues, Ronaldo B. Martins, Andreia M. Luzenti, Glaucia R. Caruso, Priscyla D. Marcato, Vania L.D. Bonato, Dario S. Zamboni, Bergman M. Ribeiro, Sônia N. Báo, Joao S. da Silva, Flávio P. Veras, Roberto N. Silva

“…In silico studies showed that AgNPs, ranging from 7 nm to 50 nm, have high affinity for spike protein from different variant of SARS-CoV-2. Our findings show that AgNPs effectively do not affect cell viability in Calu-3 cells, inhibit viral infection in Vero-E6 cells and progression of infection in vitro. …”
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Maternal exposure to deltamethrin during pregnancy and lactation impairs hippocampal learning and memory function of male offspring by ferroptosis by Shasha Huang, Wanqi Li, Dengke Wang, Huiwen Feng, Bo Wang, Xinyu Dong, Wei Zhao, Dan Liu, Yuan Wang

“…In order to further confirm the regulation mechanisms of DM, we detected ferrous ion, oxidative stress, ferroptosis related proteins, phospholipase C (PL-C)/inositol triphosphate 3 receptor (IP3R) signaling pathway, intracellular Ca2+ and calcineurin (CaN) content in vivo. …”
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One-step diagnosis of infection and lung cancer using metagenomic sequencing by Shaoqiang Li, Yangqing Zhan, Yan Wang, Weilong Li, Xidong Wang, Haoru Wang, Wenjun Sun, Xuefang Cao, Zhengtu Li, Feng Ye

“…These species exhibited significant correlations with C reaction protein (CRP) and CA153 values. Furthermore, Haemophilus influenzae was enriched in the early-stage SCC group and significantly associated with CRP values. …”
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GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma by Meng Li, Yuan Gao, Wei Zhang, Zhijun Tan, Kuo Zhang, Zhaowei Wang, Lei He, Weina Li, Chuanyang Xu, Jieyu Zhang, Desheng Wang, Kefeng Dou, Ran Zhuang, Boquan Jin, Qiang Hao, Wangqian Zhang, Shuning Wang, Jintao Gu, Lei Shang, Haichuan Su, Yingqi Zhang, Cun Zhang

“…GDF15 promotes the generation of peripherally derived inducible Treg (iTreg) cells and enhances the suppressive function of natural Treg (nTreg) cells by interacting with a previously unrecognized receptor CD48 on T cells and thus downregulates STUB1, an E3 ligase that mediates forkhead box P3 (FOXP3) protein degradation. GDF15 neutralizing antibody effectively eradicates HCC and augments the antitumor immunity in mouse.Conclusions Our results reveal the generation and function enhancement of Treg cells induced by GDF15 is a new mechanism for HCC-related immunosuppression. …”
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Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy by Xuehui Fan, Guoqiang Yang, Yinuo Wang, Haojie Shi, Katja Nitschke, Katherine Sattler, Mohammad Abumayyaleh, Lukas Cyganek, Philipp Nuhn, Thomas Worst, Bin Liao, Gergana Dobreva, Daniel Duerschmied, Xiaobo Zhou, Ibrahim El-Battrawy, Ibrahim Akin

“…Dual luciferase reporter assay coupled with gene mutation techniques identified that miR-126-3p was found to target the regulator of G-protein signaling 3 (RGS3) gene. Western blot and qPCR analyses confirmed that miR-126-3p-mimic reduced RGS3 expression in both HCMECs and hiPSC-CMs, indicating miR-126-3p inhibits RGS3 signaling. …”
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Enhancing reproductive capacity in hybrid Red Tilapia (Oreochromis niloticus ×‎ O. mossambicus) via dietary administration of date palm pollen (Phoenix dactylifera L.)‎ by Nashi K. Alqahtani, Hesham S. Ghazzawy, Roshmon Thomas Mathew, Yousef‎ Ahmed Alkhamis, Rashid Saleh Alngada, Moaheda E.H. Eissa, Sameh A. Abdelnour, Sara F. Ghanem, Layla A. Almutairi, El-Sayed Hemdan Eissa

“…The results demonstrated that fish fed diets supplemented with 20 g or 40 g of DPP exhibited significantly higher levels of total protein, albumin, and globulin compared to the control group (P < 0.01). …”
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HINT1 suppression protects against age-related cardiac dysfunction by enhancing mitochondrial biogenesis by Michio Sato, Tsuyoshi Kadomatsu, Jun Morinaga, Yuya Kinoshita, Daisuke Torigoe, Haruki Horiguchi, Sumio Ohtsuki, Shuji Yamamura, Ryoko Kusaba, Takanori Yamaguchi, Goro Yoshioka, Kimi Araki, Tomohiko Wakayama, Keishi Miyata, Koichi Node, Yuichi Oike

“…Interestingly, CR not only suppressed age-related declines in cardiac function and mitochondrial biogenesis but blocked concomitant increases in cardiac HINT1 protein levels and maintained Tfam transcription. …”
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