Passive infusion of an S2-Stem broadly neutralizing antibody protects against SARS-CoV-2 infection and lower airway inflammation in rhesus macaques. by Christopher T Edwards, Kirti A Karunakaran, Elijah Garcia, Nathan Beutler, Matthew Gagne, Nadia Golden, Hadj Aoued, Kathryn L Pellegrini, Matthew R Burnett, Christopher Cole Honeycutt, Stacey A Lapp, Thang Ton, Mark C Lin, Amanda Metz, Andrei Bombin, Kelly Goff, Sarah E Scheuermann, Amelia Wilkes, Jennifer S Wood, Stephanie Ehnert, Stacey Weissman, Elizabeth H Curran, Melissa Roy, Evan Dessasau, Mirko Paiardini, Amit A Upadhyay, Ian N Moore, Nicholas J Maness, Daniel C Douek, Anne Piantadosi, Raiees Andrabi, Thomas R Rogers, Dennis R Burton, Steven E Bosinger

“…Many of these mAbs target the conserved S2 stem region of the SARS-CoV-2 spike protein, rather than the receptor binding domain contained within S1 primarily targeted by current SARS-CoV-2 vaccines. …”
Get full text

Evaluation of cellular and physiological alterations of cells from Mytilus galloprovincialis exposed to benzisothiazolinone by Federica Impellitteri, Kristian Riolo, Giorgia Zicarelli, Miriam Porretti, Cristiana Roberta Multisanti, Giuseppe Piccione, Alessia Giannetto, Caterina Faggio

“…The most significant results obtained from these tests were the reduction of viability of haemocytes at T2 of exposure to B2, a decrease of vitality in DG cells exposed to B1 during the T1, the significant alteration in the phagocytosis activity, the upregulation of Cu/ZnSOD gene, the inhibition of the CYPY1 gene expression and upregulation of the Heat shock protein 70 (Hsp70) gene following the BIT exposure. …”
Get full text

The demographic history of the wild crop relative Brachypodium distachyon is shaped by distinct past and present ecological niches by Minadakis, Nikolaos, Williams, Hefin, Horvath, Robert, Caković, Danka, Stritt, Christoph, Thieme, Michael, Bourgeois, Yann, Roulin, Anne C.

“…Given that grasses also supply about a fifth of the world's dietary protein as cereal grains, unlocking the sources of phenotypic variation in B. distachyon is hence of prime interest in fundamental and applied research in agronomy, ecology and evolution. …”
Get full text

Meat quality and fatty acid composition of Nero d’Aspromonte native pigs: effect of rearing system and carcase weight by Meng Peng, Siria Tavaniello, Mengjun Wu, Sanije Zejnelhoxha, Marisa Palazzo, Giulia Grassi, Yongqing Hou, Giuseppe Maiorano

Get full text

CircHIPK2 recruits SRSF1 to increase TXNIP mRNA stability and promotes autophagy-dependent ferroptosis and apoptosis in myocardial ischemia-reperfusion injury by Zhu Zhang, Jinqi Hao, Qinghong Qiao, Junting Song, Yanqin Yu

“…Expression of circHIPK2, SRSF1 and TXNIP was assessed using RT-qPCR. Protein levels of autophagy markers (LC3II/LC3I, Beclin1, p62) and ferroptosis markers (GPX4, FTH1, ACSL4) were detected by Western blot. …”
Get full text

Perbedaan Status Gizi, Pola Makan, Riwayat Diare, dan Pengetahuan Gizi Seimbang pada Anak Autisme dan Non-Autisme di Jakarta by Andini Rizky Aulia, Sintha Fransiske Simanungkalit, Firlia Ayu Arini

“…Hasil: Hasil menunjukkan perbedaan signifikan pada status gizi TB/U (p-value=0,006), frekuensi konsumsi makanan pokok (p-value=0,018), protein hewani (p-value=0,014), produk olahan susu (p-value=0,001), jumlah konsumsi makanan pokok (p-value=0,016), produk olahan susu (p-value=0,003), asupan lemak (p-value=0,037), asupan serat (p-value=0,033), frekuensi diare (p-value=0,042), dan durasi diare (p-value=0,042). …”
Get full text

Stryphnodendron adstringens have a modulatory effect on inflammatory cytokines markers of in vitro activated macrophages by Ivana Beatrice Mânica da Cruz, Cibele Ferreira Teixeira, Neida Luiza Pellenz, Moisés Henrique Mastella, Verônica Farina Azzolin, Euler Esteves Ribeiro, Fernanda Barbisan

“…Extracts at concentrations of 0.49 mg/mL and 0.99 mg/mL decreased the protein and gene expression of pro-inflammatory cytokines, exhibiting the opposite effect concerning IL-10. …”
Get full text

Development of High Dietary Fiber Cereal Bar as Emergency Food Product and The Chemical-Microbiological Properties and Nutritional Content by Ani Radiati, Sumarto Sumarto, Fahmi Hafid, Emma Kamelia, Lia Nurcahyani, Dini Mariani, Siti Badriah, Tjahja Muhandri

“…Chemical analysis included dietary fiber, carbohydrates, protein, fat, minerals, and vitamins while the microbiological analysis covered total plate count (TPC) and detection of pathogenic bacteria such as Escherichia coli, Salmonella, and Staphylococcus aureus in accordance with food safety standards from the Indonesian Food and Drug Authority (BPOM). …”
Get full text

Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting by Rachel D. Altshuler, Megan A. M. Burke, Kristine T. Garcia, Kenneth Class, Raffaello Cimbro, Xuan Li, Xuan Li

“…On abstinence day 28, we found increased mRNA expression for Grm3, Opcml, and Usp9x in all sorted DS neurons, but not DS homogenate.DiscussionTogether, these data demonstrated that incubation of Meth craving was associated with time-dependent, circuit-specific, and cell type-specific gene alterations in DS involved in glutamatergic, GABAergic, opioidergic, and protein degradation signaling.…”
Get full text

WNT3A promotes the cementogenic differentiation of dental pulp stem cells through the FOXO1 signaling pathway by Dongmei Cheng, Yang Bao, Xue Wang, Haidong Xiang, Tianyuan Guo, Yong Du, Zhiyong Zhang, Han Guo

“…Subsequently, cell experiments were conducted to validate the core targets. Changes in protein expression related to the FOXO1 signaling pathway, cell cycle, and apoptosis were evaluated using western blotting (WB). …”
Get full text

Microplastic exposure induces HSP90α secretion and aggravates asthmatic airway remodeling via PI3K-Akt-mTOR pathway by Mingming Xu, Jiyuan Chen, Lin Gao, Shaoxi Cai, Hangming Dong

“…There are several cytokines involved in the inflammatory response of asthma. Heat shock protein 90α(HSP90α) is one of cytokines involving in inflammation which is a member of the HSPs family. …”
Get full text

Enteric-Coated Aspirin Induces Small Intestinal Injury via the Nrf2/Gpx4 Pathway: A Promising Model for Chronic Enteropathy by Zhang M, Xia S, Feng L, Han X, Zhang Y, Huang Y, Liu Y, Zhao K, Guan J, Tian D, Liao J, Yu Y

“…The oxidative stress levels of the small intestine were detected by immunofluorescence and immunohistochemistry. The mRNA and protein levels of inflammatory factors, tight junctions, and antioxidant defense-related genes were measured by qRT-PCR and Western Blot. …”
Get full text

Discovery of the bacterial HslV protease activators as lead molecules with novel mode of action by Aurangzeb Sana, Aurongzeb Muhammad, Shamim Shahbaz, Rashid Yasmeen, Khan Khalid Mohammed, Aziz Tariq, Alharbi Metab, Alasmari Abdullah F.

“…., the HslV protease and the HslU ATPase. These proteins come together to form a functional enzyme complex, where the C-terminal helix of each HslU subunit is inserted into the binding pocket of each HslV dimer. …”
Get full text

Optimization of the Solid-state Fermentation Process for Morchella esculenta Fermented Wheat and Analysis of Its Nutritional Components, Physicochemical Properties and Antioxidant... by Dandan GU, Xue DONG, Jinxiu ZHANG, Xiaoru WANG, Zongshuo ZHAO, Li'an WANG

“…Compared to unfermented wheat, the surface of FFW developed holes, increased grooves, and deepened wrinkles. The protein content of FFW increased by 78.79%, while the carbohydrate content decreased by 17.79%. …”
Get full text

High quality genome assembly and annotation (v1) of the eukaryotic terrestrial microalga Coccomyxa viridis SAG 216-4 by Kraege, Anton, Chavarro-Carrero, Edgar A., Guiglielmoni, Nadège, Schnell, Eva, Kirangwa, Joseph, Heilmann-Heimbach, Stefanie, Becker, Kerstin, Köhrer, Karl, Schiffer, Philipp, Thomma, Bart P. H. J., Rovenich, Hanna

“…Transcriptome-guided gene annotation resulted in the identification of 13,557 protein-coding genes, of which 68% have annotated PFAM domains and 962 are predicted to be secreted.…”
Get full text

Insight into the role of antioxidant in microbial lignin degradation: Ascorbic acid as a fortifier of lignin-degrading enzymes by Aipeng Li, Weimin Wang, Shuqi Guo, Changzhi Li, Xinying Wang, Qiang Fei

“…Proteomics analysis suggested that the enhancement in enzyme activity was more likely to attributed to the reinforcement of AA on oxidative protein folding and transportation, rather than changes in enzyme expression. …”
Get full text

Impairment of cocaine-mediated behaviours in mice by clinically relevant Ras-ERK inhibitors by Alessandro Papale, Ilaria Maria Morella, Marzia Tina Indrigo, Rick Eugene Bernardi, Livia Marrone, Francesca Marchisella, Andrea Brancale, Rainer Spanagel, Riccardo Brambilla, Stefania Fasano

“…We designed two new cell-penetrating peptides - RB1 and RB3 - that penetrate the brain and, in the micromolar range, inhibit phosphorylation of ERK, histone H3 and S6 ribosomal protein in striatal slices. Furthermore, a screening of small therapeutics currently in clinical trials for cancer therapy revealed PD325901 as a brain-penetrating drug that blocks ERK signalling in the nanomolar range. …”
Get full text

Regulatory mechanism of exogenous myo-inositol improving high temperature stress tolerance of tomato seedlings by CHEN Yan, HAN Liyuan, DU Zilong, ZHU Dongxing, ZHU Weimin, ZHANG Yingying

“…Exogenous myo-inositol increased the expression of heat response genes, antioxidant-related genes, ascorbic acid synthesis genes, and decreased the expression of heat shock protein genes. In addition, exogenous myo-inositol responded to high temperature stress by regulating abscisic acid synthesis and signaling pathways to regulate the level of ABA in tomato seedlings. …”
Get full text

Factor V H1299r (Hr2) Heterozygosity: A Risk Factor For Recurrent Implantation Failure Particularly In Non-Carriers For Factor V Leiden Mutation-A Case-Control Study by A. Seval Ozgu-Erdinc, Pınar Gulsen Coban, Nafiye Yilmaz, Zuhal Candemir, Saynur Yilmaz, Salim Erkaya

“…Study Design: A total of 279 women were recruited in this case-control study. 229 women with a history of recurrent implantation failure and 50 fertile control with no history of pregnancy losses were screened for eight specific gene mutations, regarding factor V G1691A gene (FV Leiden), FV HR2, factor II prothrombin G20210A, factor XIII V34L, PAI-1 4G/5G, MTHFR C677T, MTHFR A1298C and A3 haplotype of the endothelial cell protein C receptor gene. Results: Recurrent implantation failure group displayed a significantly higher prevalence of FV HR2 heterozygosity than fertile controls while the frequency of FV Leiden mutation was comparable between groups (p=0.011; p=0.619). …”
Get full text

Rational design of novel phenol ether derivatives as non-covalent proteasome inhibitors through 3D-QSAR, molecular docking and ADMET prediction by Miao Yuan, Hanwen Ji, Fengxin Sun, Qiang Chen, Ping Cheng

“…While the binding models between proteasome [protein data bank (PDB) code: 3MG6] and three representative compounds (15, 20, and D24) were also analyzed by using the molecular docking method. …”
Get full text