Low-Dose Decitabine Assists Human Umbilical Cord-Derived Mesenchymal Stem Cells in Protecting β Cells via the Modulation of the Macrophage Phenotype in Type 2 Diabetic Mice by Jing Xue, Yu Cheng, Haojie Hao, Jieqing Gao, Yaqi Yin, Songyan Yu, Junyan Zou, Jiejie Liu, Qi Zhang, Yiming Mu

“…An in vitro study demonstrated that DAC together with MSCs further polarized macrophages from the M1 to M2 phenotype via the PI3K/AKT pathway. Conclusion. These data unveiled that DAC prolonged the antidiabetic effect of MSCs and promoted sustainable β-cell restoration, possibly by modulating the macrophage phenotype. …”
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Epidermal stem cell derived exosomes-induced dedifferentiation of myofibroblasts inhibits scarring via the miR-203a-3p/PIK3CA axis by Shixin Zhao, Haoran Kong, Dahu Qi, Yushuang Qiao, Yu Li, Zhiming Cao, Hanwen Wang, Xuefeng He, Hengdeng Liu, Hao Yang, Suyue Gao, Tao Liu, Julin Xie

“…Mechanistically, miR-203a-3p in EpiSC-EVs plays an essential role in inhibiting PIK3CA expression and PI3K/AKT/mTOR pathway hyperactivation, thereby reducing scar formation. …”
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Inhibition of p70 Ribosomal S6K1 Protects the Myocardium against Ischemia/Reperfusion-Induced Necrosis through Downregulation of RIP3 by Hui Shang, Jinjin Shi, Jun Zhu, Yunfeng Guo, Xiaoyan Wang

“…Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining and propidium iodide (PI) staining were used to examine the apoptosis and necrosis of myocardial tissues. …”
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Cost Savings in Chronic Pain Patients Initiating Peripheral Nerve Stimulation (PNS) with a 60-Day PNS Treatment by David M. Dickerson, Hemant Kalia, Kevin E. Vorenkamp, Konstantin V. Slavin, Jonathan M. Hagedorn, Candace Gunnarsson, Eric L. Keuffel, Andrew J. Epstein, Mark Stultz, Nathan D. Crosby

“…Abstract Introduction This study evaluates the financial impact on healthcare payers when chronic pain patients initiate peripheral nerve stimulation (PNS) with a 60-day percutaneous PNS (60-Day PNS) treatment versus a conventional brief PNS trial (PNS-BT) with possible follow-on of a permanently implanted PNS system (PNS-PI). Methods Centers for Medicare & Medicaid Services (CMS) fee-for-service (FFS) data were analyzed to identify patients with at least 12 months of follow-up (median 26.4 months) who initiated PNS treatment with: (1) 60-Day PNS or (2) PNS-BT. …”
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Deciphering the role of miRNA-mRNA interactions in cerebral vasospasm post intracranial hemorrhage by Xiang Chu, Xiang Chu, Xiyan Zhu, Honghao Xu, Wenbing Zhao, Debin Guo, Xing Chen, Jinze Wu, Lei Li, Hao Wang, Jun Fei

“…Further functional enrichment and KEGG analysis revealed that these DEGs were enriched in several terms and pathways, including the PI3K/AKT/mTOR signaling pathway, oxidative phosphorylation pathway, RNA degradation, and folate biosynthesis signaling pathway. …”
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Virulence changes in Vibrio parahaemolyticus during the freezing of Penaeus chinensis by Zenghui Gao, Heer Ding, Ke Li, Qiang Zhang, Xubo Zhao, Liu Bin, Tianli Yue

“…Bacterial quantities were significantly reduced during drain and sprinkling phases, but caused by different factors. By SYTO9 and PI staining showed that washing was the main reason for the bacterial reduction at the drain phase, while the strain entering VBNC state was another reason at sprinkling phase. …”
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Salivary peptidomic profiling of chronic gingivostomatitis in cats by matrix‐assisted laser desorption/ionization‐time‐of‐flight mass spectrometry and nanoscale liquid chromatograp... by Sekkarin Ploypetch, Apisit Pornthummawat, Sittiruk Roytrakul, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sabrina Wahyu Wardhani, Sitthichok Lacharoje, Somporn Techangamsuwan

“…The 9 novel salivary peptide markers were associated with the JAK/STAT and PI3K/Akt pathways and immune responses. These potentially noninvasive biomarkers may facilitate understanding of FCGS pathophysiology and guide future therapeutic research.…”
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Taohong Siwu Decoction Exerts a Beneficial Effect on Cardiac Function by Possibly Improving the Microenvironment and Decreasing Mitochondrial Fission after Myocardial Infarction by Zhi-rong Luo, Han Li, Zhong-xin Xiao, Shui-jin Shao, Tian-tian Zhao, Yue Zhao, Fang-fang Mou, Bo Yu, Hai-dong Guo

“…Compared with the model group, THSWD significantly increased the expression of IGF-1 and bFGF, reduced collagen deposition, promoted angiogenesis, reduced cell apoptosis, and activated the PI3K/Akt signaling pathway. Notably, THSWD significantly decreased mitochondrial ROS production and Fis1 expression. …”
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Groundwater for drinking and sustainable agriculture and public health hazards of nitrate: Developmental and sustainability implications for an arid aquifer system by Boualem Bouselsal, Adel Satouh, Johnbosco C. Egbueri, Mofreh A. Hashim, Ahmed A. Arafat, Prabhu Paramasivam, Ali Alzaed, Enas E. Hussein

“…Agricultural suitability was assessed using metrics such as Sodium Adsorption Ratio (SAR), Sodium Percentage (Na%), and Permeability Index (PI), revealing a range from permissible to doubtful irrigation quality. …”
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Trade-Off Between Enzymatic Antioxidant Defense and Accumulation of Organic Metabolite Affects Salt Tolerance of White Clover Associated with Redox, Water, and Metabolic Homeostase... by Min Zhou, Yuting Wu, Yuchen Yang, Yan Yuan, Junnan Lin, Long Lin, Zhou Li

“…This study aims to investigate the difference in the mechanism of salt tolerance in relation to osmotic adjustment, enzymatic and nonenzymatic antioxidant defenses, and organic metabolites remodeling between salt-tolerant PI237292 (Trp004) and salt-sensitive Korla (KL). …”
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ITGA4 as a potential prognostic and immunotherapeutic biomarker in human cancer and its clinical significance in gastric cancer: an integrated analysis and validation by Jiaxing Zhang, Jiaxing Zhang, Gang Wang, Jie Liu, Futian Tang, Song Wang, Yumin Li, Yumin Li

“…ITGA4 played roles in cell adhesion, migration, immune regulation, and pathways like PI3K-Akt and TSC-mTOR. It showed alterations in 22 cancer types, with methylation at 9 sites inhibiting its expression. …”
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Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α by Runjie Guo, Ping Chen, Tiantian Fu, Ren Zhang, Yuan Zhu, Nange Jin, Hong Xu, Yong Xia, Xuesong Tian

“…Calcein acetoxymethylester/propidium iodide (Calcein-AM/PI) and Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were used to detect necroptotic and apoptotic ARPE19 cells, respectively. …”
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Construction of a radiogenomic signature based on endoplasmic reticulum stress for predicting prognosis and systemic combination therapy response in hepatocellular carcinoma by Huai-Yu Wu, Shu-Ya Cao, Zheng-Gang Xu, Tian- Wang, Gu-Wei Ji, Ke Wang

“…Drug sensitivity analysis found that most sensitive drugs target the phosphatidylinositol 3-kinase/ mechanistic target of rapamycin (PI3K/mTOR) signaling pathway. Further research revealed the expression of STC2 in the endothelial cells (ECs), particularly plasmalemma vesicle associated protein (PLVAP) + ECs, and may regulate the reprogramming and function of macrophages. …”
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Lipopolysaccharide (LPS) Aggravates High Glucose- and Hypoxia/Reoxygenation-Induced Injury through Activating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis in H9C2 Cardiomyo... by Zhen Qiu, Yuhong He, Hao Ming, Shaoqing Lei, Yan Leng, Zhong-yuan Xia

“…The incidence of pyroptosis was detected by calcein-AM/propidium iodide (PI) double staining kit. The concentrations of IL-1β and IL-18 in the supernatants were assessed by ELISA. …”
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The Role of Autophagy in Copper-Induced Apoptosis and Developmental Neurotoxicity in SH-SY5Y Cells by Lu Lu, Ying Zhang, Wei Shi, Qian Zhou, Zhuoqi Lai, Yuepu Pu, Lihong Yin

“…Exposure to Cu²⁺ increased autophagosome accumulation and autophagic flux blockage in terms of increased sequestosome 1 (p62/SQSTM1) and microtubule-associated protein 1 light chain 3B (LC3B) II/LC3BI expressions and inhibition of the phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR pathway. Furthermore, copper induced apoptosis, characterized by increased expressions of Bcl2 X protein (Bax), caspase 3, and Poly (ADP-ribose) polymerase (PARP) and decreased expression of B-cell lymphoma 2 (Bcl2). …”
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Predicting breast cancer prognosis using PR and PIK3CA biomarkers: a comparative analysis of diagnostic groups by Yuting Feng, Qingzhen Song, Lei Yan, Ruoqi Li, Mengqin Yang, Peng Bu, Jing Lian

“…Abstract Purpose To evaluate the prognostic significance of progesterone receptor (PR) expression and the PIK3CA mutation status in HR+/HER2 − breast cancer patients, with the goal of screening patients who may derive the greatest benefit from PI3K-targeted therapy. Methods A retrospective analysis was conducted on 152 HR+/HER2 − breast cancer patients stratified by PR expression levels and PIK3CA mutation status. …”
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Ginsenosides Rg1, Rb1 and rare ginsenosides: Promising candidate agents for Parkinson's disease and Alzheimer's disease and network pharmacology analysis by Mingchun Jiang, Jiaxin Chi, Yifan Qiao, Jinpeng Wang, Zhixin Zhang, Jia Liu, Xinhao Sheng, Liangjie Yuan

“…By analysing the existing research advances and network pharmacology results, we found that the neuroprotective effects of ginsenosides, primarily mediated through anti-inflammation, anti-apoptosis and anti-oxidative stress, etc, may be associated with the PI3K/Akt, BDNF/TrkB, MAPKs, NF-κB, Nrf2 and Wnt/β-catenin signalling pathways. …”
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Mechanistic investigation and the optimal dose based on baicalin in the treatment of ulcerative colitis–A preclinical systematic review and meta-analysis by Jinchen Chong, Zepeng Chen, Jiaze Ma, Linhai He, Yijia Zhu, Zhihua Lu, Zhengxi Qiu, Chen Chen, Yugen Chen, Feng Jiang

“…Baicalin’s actions may involve the PI3K/AKT, TLR4/NF-κB, IKK/IKB, Bcl-2/Bax, Th17/Treg, and TLRs/MyD88 pathways. …”
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Chronic NaAsO2 exposure promotes migration and invasion of prostate cancer cells by Akt/GSK-3β/β-catenin/TCF4 axis-mediated epithelial-mesenchymal transition by Zhi-Hui Zhang, Hai-Xin Yan, Ming-Dong Liu, Feng-Wen Niu, Kai Yao, Shi-Yao Feng, Xi Li, Yuan-Hua Chen, Dong-Dong Xie

“…Ly294002, a selective phosphatidylinositol 3-kinase (PI3K)/Akt inhibitor, suppressed the β-catenin/TCF4 complex activation and EMT through blocking Akt-mediated GSK-3β inactivation in the ''disruption complex'' in chronic NaAsO2 exposed DU145 and PC-3 cells. …”
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Dietary supplementation of Astragalus polysaccharide or its nanoparticles enhances testicular hemodynamics, echotexture, scrotal circumference, concentration of testosterone, estra... by Eman Fayez, Ali Salama, Mohamed Ahmed Ismail, Fady Sayed Youssef, Zaher Mohamed Rawash, MR Oshba, Haney Samir

“…Results revealed decreases (P < 0.05) in the Doppler indices (resistive index; RI, pulsatility index; PI, and systolic/diastolic; S/D) of the testicular arteries at most time points of the study in the nano APS and APS groups. …”
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