Detection of I491F and V170F rpoB mutations associated with misdiagnosis of rifampicin resistance among patients with drug-susceptible tuberculosis treatment failure, Myanmar, 2022 by Phyu Win Ei, Mi Mi Htwe, Myat Htut Nyunt, Aye Su Mon, Zaw Myint, Wint Wint Nyunt, Su Mon Win, Sandar Aung, Wai Myat Thwe, Wah Wah Aung

“…Methods: Sputum specimens from first-line TB treatment failed patients attending Tuberculosis Centers in Yangon Region during 2022 were cultured in solid media. Phenotypic drug susceptibility testing was conducted using Mycobacterial Growth Indicator Tube method. …”
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Isolation, Characterization, and Safety Evaluation of Human Skin-Derived Precursors from an Adherent Monolayer Culture System by Ru Dai, Wei Hua, Wei Chen, Lidan Xiong, Li Li, Yiming Li

“…To distinguish tSKPs from FBs, we compared their phenotypes and transcriptomes. The tumorigenicity of tSKPs and FBs was also assessed in our study. …”
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Genetics of Stay-Green Trait and Its Association with Leaf Spot Tolerance and Pod Yield in Groundnut by Rukiya Danful, Yussif Baba Kassim, Doris Kanvenaa Puozaa, Richard Oteng-Frimpong, Masawudu Abdul Rasheed, Alexander Wireko-Kena, Richard Akromah

“…Meanwhile, advances in phenotyping for disease tolerance in other crops have established a strong relationship between stay-green trait and foliar disease tolerance. …”
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Skeletal progenitor LRP1 deficiency causes severe and persistent skeletal defects with Wnt pathway dysregulation by Mohammad Alhashmi, Abdulrahman M. E. Gremida, Santosh K. Maharana, Marco Antonaci, Amy Kerr, Shijian Fu, Sharna Lunn, David A. Turner, Noor A. Al-Maslamani, Ke Liu, Maria M. Meschis, Hazel Sutherland, Peter Wilson, Peter Clegg, Grant N. Wheeler, Robert J. van ‘t Hof, George Bou-Gharios, Kazuhiro Yamamoto

“…These abnormalities, which resemble phenotypes associated with Wnt signalling pathways, result in severe and persistent skeletal defects including a severe deficit in hip joint and patella, and markedly deformed and low-density long bones leading to dwarfism and impaired mobility. …”
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Congenital muscular dystrophies and myopathies: the leading cause of genetic muscular disorders in eleven Chinese families by Bin Mao, Xiaoling Cai, Na Lin, Yulin Jiang, Na Hao, Yifang Dai, Danhua Guo, Deqin He, Huili Xue, Lingji Chen, Qianqian He, Min Zhang, Meihuan Chen, Hailong Huang, Liangpu Xu

“…Methods Essential clinical tests, such as biomedical examination, electromyography and muscle biopsy, were applied to evaluate patient phenotypes. Whole exome sequencing was used to screen for prospective variants responsible for muscular diseases, followed by co-segregation analysis in the families and prenatal diagnosis via Sanger sequencing, if appropriate. …”
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Avolition Characterizes the Chronic Fatigue Experienced in Quiescent Inflammatory Bowel Disease by Tristan Gabriel-Segard, Margherita Boltri, Mathilde Barrau, Catherine Massoubre, Stéphane Paul, Xavier Roblin

“…<b>Methods:</b> Patients presenting IBD (n = 110) in a quiescent stage of Crohn’s disease (CD) (n = 60) and ulcerative colitis (UC) (n = 50) were enrolled and classified following their declared experience of fatigue (n = 58) or not (n = 52). Patients were phenotyped using self-administered scales for fatigue experience, bowel disease disability, quality of life and mental health symptoms. …”
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Development of Speech and Communication in Polish Children with 22q11.2 Deletion Syndrome: A Cross-Sectional Study by Natalia Moćko, Marcin Rudzki, Zuzanna Miodońska, Julia Olesiak, Katarzyna Jochymczyk-Woźniak, Michał Kręcichwost

“…Background/Objectives: 22q11.2 microdeletion syndrome (22q11DS) is a genetic disease caused by aberration of chromosome 22 that results in some phenotypic features and developmental disorders. This paper presents a cross-sectional study on speech and communication of Polish children with 22q11DS. …”
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Pharmacometabolomics Enables Real-World Drug Metabolism Sciences by Fleur B. Nijdam, Marieke A. J. Hof, Hans Blokzijl, Stephan J. L. Bakker, Eelko Hak, Gérard Hopfgartner, Frank Klont, on behalf of the TransplantLines Investigators

“…To address these limitations, a more phenotypic approach would be desirable, for which pharmacometabolomics (PMx) could be useful by studying and elucidating drug metabolism in patient samples, such as blood and urine. …”
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Characterization of Probiotic Abilities of Lactobacilli Isolated from Iranian Koozeh Traditional Cheese by Tavakoli Mahmoud, Hamidi-Esfahani Zohreh, Hejazi Mohammad Amin, Azizi Mohammad Hossein, Abbasi Soleiman

“…Eight lactic isolates including Lactobacillus plantarum (MT.ZH193, MT.ZH293, MT.ZH393 and MT.ZH593), L. casei (MT.ZH493), L. pentosus (MT.ZH693), and L. fermentum (MT.ZH893 and MT.ZH993) were identified from an Iranian traditional cheese “Koozeh Paneer” using the morphological, phenotypical, biochemical and molecular characterization and then their probiotic characteristics were compared. …”
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Breg Cells in Celiac Disease Isolated or Associated to Hashimoto’s Thyroiditis by Maria Giulia Santaguida, Ilenia Gatto, Giorgio Mangino, Camilla Virili, Ilaria Stramazzo, Poupak Fallahi, Alessandro Antonelli, Patrizia Gargiulo, Giovanna Romeo, Marco Centanni

“…On the contrary, Breg memory phenotypes (CD24hiCD38− and CD24hiCD27+) significantly decreased in patients with HT + CD as compared with the isolated disorders. …”
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Inhibition of the invasive plant Ambrosia trifida by Sigesbeckia glabrescens extracts by Lijuan Yin, Guangzhong Zhang, Haoyu Zhao, Yuxin Zhang, Jiaozi Wangchen, Fanghao Wan, Bo Liu, Wanqiang Qian

“…Furthermore, we identified three main allelochemicals, coumarin, ferulic acid, and 5-aminolevulinic acid (5-ALA), in S. glabrescens extracts and revealed that the combination of these three compounds could suppress the growth of A. trifida seedlings. The phenotypes and transcriptome profiles of the seedlings treated with these chemicals were the same as those of the seedlings treated with the S. glabrescens extracts. …”
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CRISPR/Cas9-editing of PRNP in Alpine goats by Aurélie Allais-Bonnet, Christophe Richard, Marjolaine André, Valérie Gelin, Marie-Christine Deloche, Aurore Lamadon, Gwendoline Morin, Béatrice Mandon-Pépin, Eugénie Canon, Dominique Thépot, Johann Laubier, Katayoun Moazami-Goudarzi, Ludivine Laffont, Olivier Dubois, Thierry Fassier, Patrice Congar, Olivier Lasserre, Tiphaine Aguirre-Lavin, Jean-Luc Vilotte, Eric Pailhoux

“…Three founders carried only genome-edited alleles and were phenotypically indistinguishable from their wild-type counterparts. …”
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Molecular characterization of putative antibiotic resistance determinant and virulence factors genes of Acinetobacter baumannii strains isolated from intensive care unit patients i... by Faris A. Alanazi, Khalid S. Almaary, Turki M. Dawoud, Mohamed A. El-Tayeb, Yahya B. Elbadawi, Ayman S. Mubarak, Ali M. Somily

“…Method: The A. baumannii isolates were confirmed by the VITEK-2 automated system and 16S rRNA phylogenetic relatedness. The phenotypic and genotypic resistance patterns of these isolates were also analyzed using conventional and molecular methods. …”
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Intrahepatic levels of microbiome-derived hippurate associates with improved metabolic dysfunction-associated steatotic liver disease by Maxime Deslande, Francesc Puig-Castellvi, Inés Castro-Dionicio, Romina Pacheco-Tapia, Violeta Raverdy, Robert Caiazzo, Guillaume Lassailly, Audrey Leloire, Petros Andrikopoulos, Yasmina Kahoul, Nawel Zaïbi, Bénédicte Toussaint, Frédérik Oger, Nicolas Gambardella, Philippe Lefebvre, Mehdi Derhourhi, Souhila Amanzougarene, Bart Staels, François Pattou, Philippe Froguel, Amélie Bonnefond, Marc-Emmanuel Dumas

“…Methods: We characterised liver biospies from 318 patients with obesity using RNAseq and metabolomics in liver and plasma to derive associations among hepatic hippurate, hepatic gene expression and MASLD and phenotypes. To test a potential beneficial role for hippurate in hepatic insulin resistance, we profile the metabolome of (IHH) using ultra-high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (UHPLC-MS/MS), and characterised intracellular triglyceride accumulation and glucose internalisation after a 24 h insulin exposure. …”
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Genotype-environment interactions on growth and inner shell color variation in Hyriopsis cumingii cultured in different iron-level substrates by Xuefeng Lv, Zhiyan Wang, Quanzhi Bao, Changzhi Li, Tingting Lu, Yuyang Wu, Chuntong Liu, Zhiyi Bai

“…The anal angle radial rib length (AARRL) displayed stronger genetic and phenotypic correlations with body weight (BW) and shell width (SW) compared to shell height (SH) and total shell height (TSH). …”
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Sotorasib resistance triggers epithelial-mesenchymal transition and activates AKT and P38-mediated signaling by Raquel Arantes Megid, Guilherme Gomes Ribeiro, Izabela Natalia Faria Gomes, Ana Carolina Laus, Letícia Ferro Leal, Letícia Ferro Leal, Luciane Sussuchi da Silva, Abu-Bakr Adetayo Ariwoola, Josiane Mourão Dias, Rui Manuel Reis, Rui Manuel Reis, Renato Jose da Silva-Oliveira, Renato Jose da Silva-Oliveira

“…The aim of this study was to generate a sotorasib-resistant cell line via selective pressure and systematically examine the molecular and phenotypic alterations caused by resistance.MethodsMutant NCI-H358 (KRASG12C) were exposed to incremental doses (2–512 nM) of sotorasib. …”
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A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans by Fruzsina Kovács, Ágnes Jakab, Noémi Balla, Zoltán Tóth, Dávid Balázsi, Lajos Forgács, Andrea Harmath, Aliz Bozó, Ágota Ragyák, László Majoros, Renátó Kovács

“…To gain deeper insights into this relationship, phenotypic and transcriptomic analyses were conducted in the presence of the primary P. aeruginosa quorum-sensing molecule, N-(3-oxododecanoyl)-l-homoserine lactone (HSL), against C. auris, with the results compared to those of C. albicans. …”
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Increasing trend of antimicrobial resistance among methicillin-resistant Staphylococcus aureus strains in Southwest Finland, 2007–2016: An analysis of shifting strain dynamics and... by Jaakko Silvola, Kirsi Gröndahl-Yli-Hannuksela, Tiina Hirvioja, Kaisu Rantakokko-Jalava, Mari Kanerva, Kari Auranen, Harri Marttila, Jenna Junnila, Jaana Vuopio

“…AMR trends and risk factors were identified by associating resistant phenotypes with patient demographics. Results: A total of 983 new MRSA cases were identified between 2007 and 2016. …”
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Influence of sacroiliac joint variation on clinical features of axial spondyloarthritis: a comparative analysis by Denis Poddubnyy, Torsten Diekhoff, Sevtap Tugce Ulas, Fabian Proft, Valeria Rios Rodriguez, Judith Rademacher, Juliane Greese, Katharina Ziegeler, Carolina Dominguez Aleixo, Maximilian Lindholz

“…Sex-stratified analysis revealed no statistically significant results.Conclusion Our analysis suggests that clinical phenotypes do not significantly differ between patients with axSpA with and without atypical joints.…”
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Molecular insight into reproductive toxicity and transgenerational effects of Cadmium exposure on Drosophila melanogaster by Yi Pan, Ke Fan, Linhao Zong, Yantao Luo, Xin Ni, Dong Chen, Jinjun Qian, Fei Ma, Jie Cheng, Miao Guan

“…At the epigenetic level, Cd could perturb fecundity across generations by modulating Dnmt2 expression, a pivotal regulator of methylation processes. Moreover, despite phenotypic recovery in F4, persistent molecular changes indicate enduring toxicity, highlighting the need for vigilance against environmental Cd contamination and its long-term effects. …”
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