Conserved LIR-specific interaction of Sigma-1 receptor and GABARAP by Marius Wilhelm Baeken, Maximilian Christ, Daniel Schmitt, Wencke Trein, Heike Nagel, Albrecht Martin Clement, Hagen Körschgen, Christian Behl

“…Biochemically, we uncovered a GABARAP-σ1R interaction depending on this motif via peptide array analysis and confirmed this via immunoprecipitation, co-localization, and proximity ligation assays. …”
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Elucidation of short linear motif-based interactions of the MIT and rhodanese domains of the ubiquitin-specific protease 8 by Aimiliani Konstantinou, Julia K. Varga, Alicia Córdova-Pérez, Leandro Simonetti, Lidia Gomez-Lucas, Ora Schueler-Furman, Norman E. Davey, Yogesh Kulathu, Ylva Ivarsson

“…Using the motif information, we predict binding sites within known USP8 interactors and substrates and validate interactions through peptide array analysis. Our findings demonstrate that both the USP8 MIT and rhodanese domains are peptide-binding domains that can be bound by degenerate and distinct binding motifs. …”
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EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation by Bin Shi, Ping Yang, Huaijin Qiao, Jinchen He, Bin Song, Hao Bai, Fengdi Jiang, Yining Zhang, Qian Li, Tao Yan, Wenlin Tu, Daojiang Yu, Shuyu Zhang

“…Notably, VPS41 overexpression promotes KAI1 lysosomal degradation, protecting against radiation‐induced apoptotic cell death. Peptide array analysis pinpoints the VPS41‐KAI1 interaction through the K263 residue, consistent with AlphaFold prediction. …”
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