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A comprehensive review of biological and genetic control approaches for leishmaniasis vector sand flies; emphasis towards promoting tools for integrated vector management.
Published 2025-01-01“…This current review identified several promising methods, including genetically modified sand flies, using transgenic approaches by taking advanced gene editing tools like Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) and genetic modification of symbiotic microorganisms for controlling sand fly populations, which appeared to be proven under laboratory and field settings.…”
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22
ParSite is a multicolor DNA labeling system that allows for simultaneous imaging of triple genomic loci in living cells.
Published 2025-01-01“…The insertions of 16 base-pair palindromic ParSc and ParSm into genomic loci allow dual-color DNA imaging in living cells. …”
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Distribution and Characteristics of Bacteria Isolated from Cystic Fibrosis Patients with Pulmonary Exacerbation
Published 2022-01-01“…Molecular typing of S. aureus and P. aeruginosa isolates was carried out by spa typing and repetitive extragenic palindromic element PCR. Results. In a total of 129 isolates, the most prevalent organisms were S. aureus (55.3%) and P. aeruginosa (41.7%). …”
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The Potential Use of the CRISPR-Cas System for HIV-1 Gene Therapy
Published 2019-01-01“…The CRISPR- (clustered regularly interspaced short palindromic repeat-) Cas system is a genome-editing method which uses a guide RNA, a complementary sequence to the interested site, recruiting a nuclease that can break the viral or the host cell genetic material. …”
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CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens
Published 2022-01-01“…Recently, clustered regularly interspaced short palindromic repeats (CRISPR)-based programmable technology has emerged to challenge the unmet criteria of conventional methods. …”
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Halothermophilic Cas9 and dCas9 Interaction with Variety sgRNA In-silico
Published 2025-01-01“…Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a highly effective method for genetic editing; however, its use in halothermophilic bacteria remains limited. …”
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Detection of CRISPR cassettes and cas genes in the Arabidopsis thaliana genome
Published 2019-11-01“…The discovery of CRISPR-Cas systems (clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins), which implement the adaptive immunity function in prokaryotes, raises the question whether such a mechanism of antiviral protection could be caught up by evolution and used by representatives of eukaryotes (in particular, plants). …”
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Complete chloroplast genomes of 13 species of the Impatiens genus for genomic features and phylogenetic relationships studies
Published 2025-02-01“…SSRs mainly consist of A/T repeats and AT/AT repeats, while INEs mainly consist of positive repeats and palindromic repeats. The frequency of codon usage was essentially the same, with a total of 31 high-frequency codons detected, the vast majority ending in A/U. …”
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CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects
Published 2025-02-01“…Genome editing technology, specifically with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) tools, has opened new possibilities for trait development in cotton. …”
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An electrolyte-gated transistor for the monitoring of a CRISPR/Cas activity
Published 2025-05-01“…Reverse transcriptase-polymerase chain reaction (RT-PCR) remains the gold standard, but clustered regularly interspaced short palindromic repeats linked to a Cas endoribonuclease particle (CRISPR/Cas) have recently revolutionized the recognition step of two RNAs, i.e. the CRISPR-RNA (crRNA) and the target, providing a much better selectivity compared to the naked hybridization on which RT-PCR is based. …”
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31
Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2
Published 2025-01-01“…In this study, a recombinant Herpesvirus of Turkeys (HVT) vaccine expressing H9-HA was constructed using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology. In the construction of HVT-EGFP-HA recombinant virus, nonhomologous end joining (NHEJ) is a much more efficient strategy compare to Homology-directed recombination (HDR). …”
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Staphylococcus aureus nt5 gene mutation through CRISPR RNA-guided base editing weakens bacterial virulence and immune evasion
Published 2025-12-01“…Herein, to reveal nt5 gene role in drug resistance and infection ability of S. aureus, we performed nt5C166T gene mutation using a clustered regulatory interspaced short palindromic repeat ribonucleic acid (RNA)-guided base editing system to investigate the lose-of-function of NT5 protein. …”
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From bench to bedside: Developing CRISPR/Cas-based therapy for ocular diseases
Published 2025-03-01“…The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) has emerged as a promising tool for treating ocular diseases through gene editing and expression regulation. …”
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Impact of predictive selection of LbCas12a CRISPR RNAs upon on‐ and off‐target editing rates in soybean
Published 2024-08-01“…Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. …”
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Effects of information on consumer attitudes towards gene-edited foods: a comparison between livestock and vegetables
Published 2021-03-01“…Japanese food producers and researchers are optimistic about the application of the clustered regularly interspaced short palindromic repeats (CRISPR) approach to food. Due to the strict regulations regarding genetically modified (GM) food, GM crops are not commercially cultivated in Japan. …”
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HP1 Promotes the Centromeric Localization of ATRX and Protects Cohesion by Interfering Wapl Activity in Mitosis
Published 2025-01-01“…Methods: The clustered regularly interspaced short palindromic repeats with CRISPR-associated protein 9 (CRISPR-Cas9) system and overexpression approaches were employed alongside immunofluorescence to investigate the mechanism of ATRX localization at the centromere. …”
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Detection of CRISPR‒Cas and type I R–M systems in Klebsiella pneumoniae of human and animal origins and their relationship to antibiotic resistance and virulence
Published 2025-02-01“…ABSTRACT The clustered regularly interspaced short palindromic repeats (CRISPR)‒CRISPR-associated protein (Cas) and restriction‒modification (R–M) systems are important immune systems in bacteria. …”
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Regulation of human papillomavirus E6 oncoprotein function via a novel ubiquitin ligase FBXO4
Published 2025-02-01“…Therefore, to identify these novel ubiquitin ligases, we performed high-throughput human siRNA library screen against ubiquitin ligases in clustered regularly interspaced palindromic repeat (CRISPR)-edited E6AP-knockout human embryonic kidney (HEK) 293 cells, stably expressing green fluorescent protein (GFP)-tagged HPV-18E6. …”
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