Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer by Xiaowei Cao, Zhenyu Wang, Liyan Bi, Jie Zheng

“…The difference spectra among different types of NSCLC were compared, and analysis result revealed their intrinsic difference in types and contents of nucleic acids, proteins, carbohydrates, amino acids, and lipids. …”
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Fourier Transform Infrared Spectroscopy Analysis as a Tool to Address Aβ Impact on Extracellular Vesicles by Margarida Vaz, Tânia Soares Martins, Kevin Leandro, Luís Pereira de Almeida, Odete A. B. da Cruz e Silva, Alexandra Nunes, Ana Gabriela Henriques

“…Specifically, Aβ seems to cause alterations in protein phosphorylation and in the nucleic acids content of extracellular vesicles. These findings support that Aβ’s role in Alzheimer’s disease pathology may be mediated by extracellular vesicles and highlight FTIR’s potential for advancing Alzheimer’s disease research and clinical applications.…”
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Non-viral systems for intracellular delivery of genome editing tools by I. H. Shaikhutdinov, P. V. Ilyasov, O. V. Gribkova, L.  V. Limareva

“…Therefore, the search for novel approaches to delivering proteins and nucleic acids into cells is a relevant priority. This work reviews abiotic vectors and systems for delivering genome editing tools into target cells, including liposomes and solid lipid particles, other membrane-based vesicles, cell-penetrating peptides, micelles, dendrimers, carbon nanotubes, inorganic, polymer, metal and other nanoparticles. …”
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Characterization of Malignant Melanoma Using Vibrational Spectroscopy by Ziad Hammody, Ranjit Kumar Sahu, Shaul Mordechai, Emanuela Cagnano, Shmuel Argov

“…The comparative analysis of various parameters calculated from the spectral data of the normal epidermis and the abnormal regions showed that the changes in the nucleic acids was a significant indicator of the abnormal nature of the tissues. …”
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Electroporation of mouse embryonic stem cells with Neon device by I. P. Savchenkova, A. A. Savchenkova

“…Electroporation of mouse embryonic stem cells with the new-generation device can bean effective method for in vitro insertion of nucleic acids into the cells of interest to the researcher.…”
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CLARITY and Light-Sheet microscopy sample preparation in application to human cerebral organoids by T. A. Shnaider, I. E. Pristyazhnyuk

“…This method is based on a special sample preparation, during which lipids are removed from cells and replaced with hydrogel compounds such as acrylamide, while proteins and nucleic acids remain intact. CLARITY provides researchers with a unique opportunity to study three-dimensional biological structures while preserving their internal organization, including whole animals or embryos, individual organs and artificially grown organoids, in particular cerebral organoids. …”
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Construction of a pathogenic microorganism detection method based on third-generation nanopore sequencing data by Guoqin Mai, Jiayi Chen, Min Zhang, Wanyue Zhang, Yuting Luo, Ying Dai

“…First, a comprehensive and penetrating reference database of nucleic acids from pathogenic microorganisms was constructed, and sequence alignment analysis was performed via nanopore sequencing of metagenomic data collected from 40 patients with lower respiratory tract infection symptoms. …”
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Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes by SUN Jinyue, PAN Jianeng, CHEN Yiying, LIU Rencheng, ZHOU Wenwen

“…Additionally, the Fe + SP + L treatment led to increased lipid peroxidation and ROS production and decreased proportion of GSH, thereby damaging the integrity of the cell membrane, increasing the permeability of the cell membrane, decreasing the membrane potential, increasing the leakage of intracellular nucleic acids and proteins, and ultimately resulting in the death of bacteria. …”
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Regenerative activity of stomach epithelial cells at small-intestinal obstruction by V. Ye. Milyukov, K. K. Nguyen

“…Metabolic processes in cells of epithelium and stomach mucosal glands were studied by Einarson method of total nucleic acids detection. Test results were estimated by quantitative microspectrophotometry and computer software allowing to define the number of the area units of the study object occupied by cytochemical reaction products. …”
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Use of ascorbic acid (vitamin C) and alpha tocopherol (vitamin E) as adjuvants in the treatment of neuropathic pain by Fernando Antonio Silva de Azevedo-Filho, Yasmim Machado Chaves de Castro, Marcos Pinho Cerqueira, Thiago Arruda Rodrigues, Yussef Ali-Abdouni, Patrícia Maria de Moraes Barros Fucs

“…BACKGROUND AND OBJECTIVES: High concentrations of free radicals can damage nucleic acids, proteins and lipids and their accumulation may be involved in hypersensitivity to pain. …”
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Comparison of clinical metagenomics with 16S rDNA Sanger sequencing for the bacteriological diagnosis of culture-negative samples by Camille d’Humières, Skerdi Haviari, Marie Petitjean, Laurène Deconinck, Signara Gueye, Nathan Peiffer-Smadja, Lynda Chalal, Naima Beldjoudi, Geoffrey Rossi, Yann Nguyen, Charles Burdet, Ségolène Perrineau, Diane Le Pluart, Roza Rahli, Michael Thy, Piotr Szychowiak, Xavier Lescure, Véronique Leflon-Guibout, Victoire de Lastours, Etienne Ruppé

“…Clinical metagenomics (CMg), i.e. the sequencing of a sample’s entire nucleic acids, may allow for the identification of bacteria not detected by conventional methods. …”
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Efficiency of Automated Viral RNA Purification for Pediatric Studies of Dengue and Zika in Hyperendemic Areas by Sandra L. Delgado, Piedad M. Perilla, Doris M. Salgado, María Clemencia Rojas, Carlos F. Narváez

“…The isolation of nucleic acids is a critical and limiting step for molecular assays, which prompted the arrival in Colombia of automated purification instruments during the SARS-CoV-2 pandemic. …”
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Active surveillance of avian influenza in the southwestern Poyang Lake area, China: Analyzing changes in wholesale and frozen fresh retail markets post-policy implementation by Wentao Song, Zhiqiang Deng, Fenglan He, Kang Fang, Lintao Sheng, Jingwen Wu, Junling Tu, Kun Zhou, Xi Wang, Wei Wang, Liu Yi, Kangguo Li, Buasiyamu Abudunaibi, Ping Zhang, Hui Li, Tianmu Chen

“…The type A avian influenza virus nucleic acids were detected using real-time reverse transcription polymerase chain reaction (real-time RT-PCR), and a triplex real-time RT-PCR assay kit specific for H5/H7/H9 RNA was utilized on the influenza A-positive samples to differentiate among the avian influenza virus subtypes. …”
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Positive Pneumocystis jirovecii Sputum PCR Results with Negative Bronchoscopic PCR Results in Suspected Pneumocystis Pneumonia by Kelly Pennington, John Wilson, Andrew H. Limper, Patricio Escalante

“…The diagnostic standard for Pneumocystis jirovecii pneumonia (PCP) is direct microscopic identification; however, in recent years, polymerase chain reaction (PCR) from bronchoalveolar lavage (BAL) samples to detect Pneumocystis nucleic acids has proven to be more sensitive and specific. …”
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Application of the weighted histogram method for calculating the thermodynamic parameters of the formation of oligodeoxyribonucleotide duplexes by I. I. Yushin, V. M. Golyshev, D. V. Pyshnyi, A. A. Lomzov

“…To date, many derivatives and analogs of nucleic acids (NAs) have been developed. Some of them have found uses in scientific research and biomedical applications. …”
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Development and validation of highly sensitive multiplex real-time RT-PCR assay for detection of classical swine fever virus genome by A. S. Sadchikova, A. S. Igolkin, R. S. Chernyshev, A. A. Kozlov, I. S. Kolbin, A. V. Sprygin, D. A. Biryuchenkov, I. A. Chvala, A. Mazloum

“…Modified primers (locked nucleic acids containing conformationally blocked nucleosides) providing a higher affinity to the DNA matrix and physico-chemical stability and a FAM-labeled TaqMan probe were selected for 5’-untranslatedregion of the genome. …”
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Decoding oral cancer: insights from miRNA expression profiles and their regulatory targets by Xin Wang, Xin Wang, Shuang Zhang, Shuang Zhang, Shuyi Wang, Tao Cao, Tao Cao, Hong Fan, Hong Fan

“…Functional annotation and pathway enrichment analyses highlighted significant involvement of DEmiRs and their target genes in cell cycle-related processes, including enrichment in the nucleus, transcription factor activity, regulation of nucleosides, nucleotide and nucleic acids, cell growth and/or maintenance, mitotic cell cycle, mitotic M-M/G1 phases an DNA replication. …”
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Inactivation of the TIM complex components leads to a decrease in the level of DNA import into Arabidopsis mitochondria by T. A. Tarasenko, K. D. Elizova, V. I. Tarasenko, M. V. Koulintchenko, Yu. M. Konstantinov

“…It has been known that nucleic acids enter plant organelles through alternative channels, depending on the size of the imported molecules. …”
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Metabolic profile of blood serum in experimental arterial hypertension by A. A. Seryapina, A. A. Malyavko, Yu. K. Polityko, L. V. Yanshole, Yu. P. Tsentalovich, A. L. Markel

“…To determine specific markers of diseases, various omics technologies are applied, including metabolomics, which makes it possible to evaluate the content of low-molecular compounds – amino acids, lipids, carbohydrates, nucleic acids fragments – in biological samples available for clinical analysis (blood and urine). …”
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Circulating miRNAs as Biomarkers for Prostate Cancer Diagnosis in Subjects with Benign Prostatic Hyperplasia by Wei Jin, Xiang Fei, Xia Wang, Fangjie Chen, Yan Song

“…Body fluids often contain freely circulating nucleic acids, many of which can be exploited as noninvasive tools for the diagnosis of cancer as well as for clinical prognostication. …”
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