Pathway Analysis of Metabolic Syndrome Using a Genome-Wide Association Study of Korea Associated Resource (KARE) Cohorts by Unjin Shim, Han-Na Kim, Yeon-Ah Sung, Hyung-Lae Kim

“…Through pathway analysis of MetS, pathways related with PDGF, mitogen-activated protein kinase, and PPAR signaling, as well as nucleic acid binding, protein secretion, and DNA repair, were identified. …”
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Cutting-edge: bionanomaterial solutions in the battle against Severe Acute Respiratory Syndrome Coronavirus 2 by U. Saeed, R. Uppal, M. H. Najmi, I. Fazal, A. A. Khan, Z. Z. Piracha, M. R. Uppal, H. N. Ijaz, D. U. Ozsahin, B. Uzun, I. Ozsahin

“…Their role in automating nucleic acid extraction and purification has proven indispensable, particularly in the diagnostic milieu of SARS-CoV-2. …”
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Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia by Babu Kanti Nath, Suman Das Gupta, Saranika talukder, Nasrin Sultana Tonu, Shane R. Raidal, Jade K. Forwood, Subir Sarker

“…Virus enrichment and nucleic acid extraction were performed on the faecal samples, followed by high‐throughput sequencing at the Australian Genome Research Facility (AGRF). …”
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Optimization of Fermentation Conditions for Increasing Erucamide Content in <i>Bacillus megaterium</i> Using Several Accelerants by Hao Zhao, Yudan Xie, Zhu Li, Longfeng Wei, Renli Ai

“…The mechanism of membrane metabolism in the L2 strain was further investigated, and our data suggested that the conductivity, nucleic acid and protein content, and β-galactosidase activity of L2 were increased significantly after treatment with accelerants, indicating that the accelerants changed the cell membrane permeability of the L2 strain but did not harm or kill the bacteria. …”
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Characterizations of cytokines and viral genomes in serum of patients with Dabie bandavirus infection by Zefeng Dong, Man Yuan, Yueping Xing, Hongkai Zhang, Qiang Shen

“…We detected and analyzed the cytokine concentrations and viral genomes in the serum samples of these patients to identify the possible causes of the patients’ deaths and to analyze the viral genetic characteristics.MethodsBlood serum specimens were obtained from the 10 individuals with Dabie bandavirus infection in Suzhou City. The specific nucleic acid of Dabie bandavirus was detected using a real-time reverse transcription polymerase chain reaction assay (RT-qPCR). …”
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The quest for environmental analytical microbiology: absolute quantitative microbiome using cellular internal standards by Chunxiao Wang, Yu Yang, Xiaoqing Xu, Dou Wang, Xianghui Shi, Lei Liu, Yu Deng, Liguan Li, Tong Zhang

“…Abstract Background High-throughput sequencing has revolutionized environmental microbiome research, providing both quantitative and qualitative insights into nucleic acid targets in the environment. The resulting microbial composition (community structure) data are essential for environmental analytical microbiology, enabling characterization of community dynamics and assessing microbial pollutants for the development of intervention strategies. …”
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Pathogenetic therapeutic approaches for endocrine diseases based on antisense oligonucleotides and RNA-interference by Olga Golounina, Ildar Minniakhmetov, Ramil Salakhov, Rita Khusainova, Ekaterina Zakharova, Igor Bychkov, Natalia Mokrysheva

“…Molecular therapy uses nucleic acid-based therapeutics agents and becomes a promising alternative for disease conditions unresponsive to traditional pharmaceutical approaches. …”
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Treatment of Hepatitis C Post-Liver Transplantation Could Mitigate Discard Rates of Hepatitis C-Positive Deceased Donor Livers and Expand the Donor Pool by Jennifer Keller, Gary Marklin, Obi Okoye, Roshani Desai, Tej Sura, Ajay Jain, Chintalapati Varma, Mustafa Nazzal

“…All deceased liver donors with HCV antibody or nucleic acid positive results were evaluated. Results. …”
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Tuberculous Otitis Media: A Mysterious Ailment by Saravana Selvan Velmurugan, Vivek Mariappan, Mohamed Siddique Samsudeen, Suresh Kumar Narayanan, Rhea Mathai, Tany James Maria

“…The granulation tissue from all the patients sent for Histopathology examination (HPE) and Cartridge Based Nucleic Acid Amplification Test (CBNAAT) examination. …”
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Antibacterial, Antifungal, and Antidiabetic Effects of Leaf Extracts from Persea americana Mill. (Lauraceae) by Mercy Makopa, Benjamin Mangiza, Benjamin Banda, Winnie Mozirandi, Molly Mombeshora, Stanley Mukanganyama

“…Only the acetone extract effected nucleic acid leakage. Screening of extracts’ potential to inhibit the activity of α-glucosidase was carried out spectrophotometrically following the production of p-nitrophenol from p-nitrophenol-glucopyranoside (substrate) at a wavelength of 405 nm. …”
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Allele-specific PCR with fluorescently labeled probes: criteria for selecting primers for genotyping by V. A. Devyatkin, A. A. Shklyar, A. Zh. Fursova, Yu. V. Rumyantseva, O. S. Kozhevnikova

“…In this article, we discuss in detail basic principles of the method, the processes that influence the result of genotyping, criteria for selecting optimal primers and probes, and the use of locked nucleic acid modifications in oligonucleotides as well as provide a protocol for the selection of primers and probes and for PCR by means of rs11121704 as an example. …”
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Occult Hepatitis B Infection among Hemodialysis Patients in Tabriz, Northwest of Iran: Prevalence and Mutations within the S Region by Narges Eslami, Vahdat Poortahmasebi, Javid Sadeghi, Reza Ghotaslou, Bahram Niknafs, Hossein Bannazadeh Baghi, Mahin Ahangar Oskouee

“…HBV serologic screening approaches need to be renewed to cover nucleic acid testing in the setting of hemodialysis and all the other high-risk groups associated with it (i.e., blood and organ donors).…”
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Antipseudomonal β-Lactams Resistance in Iran by Mohammad Mahdi Rabiei, Keivan Asadi, Shervin Shokouhi, Mohammad Javad Nasiri, Ilad Alavi Darazam

“…However, the widespread usage of these approaches undoubtedly needs reliable molecular and nucleic acid-based devices, which are both affordable and available.…”
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Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital by Funda Şahin, Nida Özcan, Erdal Özbek, Selahattin Atmaca, Hakan Temiz

“…The Biospeedy viral nucleic acid isolation kit (Bioeksen, Türkiye) was used for the isolation of genetic material. …”
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Magnetic Nanoparticle Based Nonviral MicroRNA Delivery into Freshly Isolated CD105+ hMSCs by Anna Schade, Paula Müller, Evgenya Delyagina, Natalia Voronina, Anna Skorska, Cornelia Lux, Gustav Steinhoff, Robert David

“…Our work may become crucial for virus-free introduction of therapeutic miRs as well as other nucleic acids in vivo. Moreover, in the field of targeted stem cell therapy nucleic acid delivery prior to transplantation may allowfor initial cell modulation in vitro.…”
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An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-Reactivity with SARS Coronavirus by David M Patrick, Martin Petric, Danuta M Skowronski, Roland Guasparini, Timothy F Booth, Mel Krajden, Patrick McGeer, Nathalie Bastien, Larry Gustafson, Janet Dubord, Diane MacDonald, Samara T David, Leila F Srour, Robert Parker, Anton Andonov, Judith Isaac-Renton, Nadine Loewen, Gail McNabb, Alan McNabb, Swee-Han Goh, Scott Henwick, Caroline Astell, Jian Ping Guo, Michael Drebot, Raymond Tellier, Francis Plummer, Robert C Brunham

“…BACKGROUND: In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV).…”
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Naked-Eye Molecular Testing for the Detection of <i>Xylella fastidiosa</i> in Mallorca (Balearic Island) Almond Orchards by Colorimetric LAMP by Amoia Serafina Serena, Ana Falcón-Piñeiro, Milica Pastar, José Manuel Garcìa-Madero, Nicoletta Contaldo, Mikael Muegge, Stéphane Compant, Pasquale Saldarelli, Angelantonio Minafra

“…Molecular detection through isothermal amplification reactions enables simplified instrumentation and the use of raw nucleic acid extracts. Colorimetric loop-mediated isothermal amplification (cLAMP) was applied to rapidly detect <i>Xf</i> in naturally infected almonds on Mallorca Island (Spain), using a quick crude sap extraction without DNA purification. …”
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Ticks and Tick-Borne Pathogens in Wild Animals and Birds from Two Rehabilitation Facilities in Greece by Dimitrios Vourvidis, Georgia Tzouganatou, Sokratis Perdikaris, Evangelia Kofidou, Beatriz Martinez-Gonzalez, Mary Emmanouil, Emmanouil Papadogiannakis, Anastasia Komnenou, Emmanouil Angelakis

“…., <i>Bartonella</i> spp., or viral nucleic acid was found. Phylogenetic analysis revealed close genetic relationships between the detected <i>Rickettsia</i> species and those previously reported in neighboring regions. …”
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Molecular Epidemiology of SARS-CoV-2 within Accra Metropolis Postlockdown by Frank T. Aboagye, Lawrence Annison, Henry Kwadwo Hackman, Maame E. Acquah, Yvonne Ashong, Isaac Owusu-Frimpong, Bill C. Egyam, Sharon Annison, George Osei-Adjei, Samuel Antwi-Baffour

“…Samples were subjected to nucleic acid extraction and followed by real-time polymerase chain reaction for the detection and quantification of SARS-CoV-2 RNA. …”
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Prognostic importance of human leukocyte antigen DRβ1 gene and protein tyrosine phosphatase nonreceptor Type 22 gene polymorphism in rheumatoid arthritis by Pramod Kumar Verma, Usha Singh, Shyam Kumar Saraf, Narendra Kumar Singh

“…HLA DRβ1 typing was done by low-resolution sequence-specific primer polymerase chain reaction and auto-antibodies (Abs) (rheumatoid factor [RF], anti-cyclic citrullinated peptide 2 (CCP2) Ab, anti-nuclear Ab, double-stranded deoxyribose nucleic acid, anticardiolipin Ab) were done by enzyme-linked immunosorbent assay. …”
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