Phytochrome-mediated shade avoidance responses impact the structure and composition of the bacterial phyllosphere microbiome of Arabidopsis by James A. O’Rourke, Stacey A. Vincent, Isabel E. I. Williams, Eleanor L. Gascoyne, Paul F. Devlin

“…A comparison of responses in both wild-type plants and phytochrome mutants, which inherently exhibit a shade-avoidance phenotype, revealed both indirect responses to host plant physiology and direct responses to light among the microbiota. …”
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Engineering a promiscuous prenyltransferase for selective biosynthesis of an undescribed bioactive cannabinoid analog by Qin Yan, Yue-Gui Chen, Xiao-Wen Yang, An Wang, Xiao-Ping He, Xue Tang, Hong Hu, Kai Guo, Zong-Hua Xiao, Yan Liu, Sheng-Hong Li

“…An efficient construct is established by incorporating an additional copy of AscC, resulting in a yield of 14.85 ± 0.91 mg/L of compound 4. Two mutants, L180Y and L180F, are engineered to selectively produce compound 4. …”
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The N-Terminal Domain of Y-Box Binding Protein-1 Induces Cell Cycle Arrest in G2/M Phase by Binding to Cyclin D1 by Payal Khandelwal, Mythili K. Padala, John Cox, Ramareddy V. Guntaka

“…The abnormalities seen in heterozygous mutants could be attributed to a dominant negative effect exerted by the disrupted YB-1 allele product. …”
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Establishment of a Breeding Approach Combined with Gamma Ray Irradiation and Tissue Regeneration for Highbush Blueberry by Xuan Yu, Haidi Yuan, Yihong Jin, Chuizheng Xia, Jiani Zhu, Jiali Che, Jiao Yang, Xiaofei Wang, Bingsong Zheng, Shufang Yang, Cristian Silvestri, Fuqiang Cui, Jianfang Zuo

“…The height of M1 plants quantitatively decreased with increasing irradiation doses. To evaluate the mutants induced by each irradiation dose, whole-genome resequencing was conducted on individuals from each dose group, revealing significant genomic alterations at the 80 Gy dose. …”
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Codeine 3-O-demethylase catalyzed biotransformation of morphinan alkaloids in Escherichia coli: site directed mutagenesis of terminal residues improves enzyme expression, stability... by Garrick W. K. Spencer, Xu Li, Kenny W. L. Lam, George Mutch, Fiona H. Fry, Sally L. Gras

“…Whole cell biotransformation performance was significantly improved in Escherichia coli expressing codeine 3-O-demethylase mutants, with a ~ 2.8-fold increase in the production of oripavine from thebaine and ~ 1.3-fold increase in the production of morphine from codeine. …”
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MicroRNA-3145 as a potential therapeutic target for hepatitis B virus: inhibition of viral replication via downregulation of HBS and HBX by Amrizal Muchtar, Amrizal Muchtar, Daichi Onomura, Daichi Onomura, Dan Ding, Dan Ding, Hironori Nishitsuji, Kunitada Shimotohno, Shunpei Okada, Keiji Ueda, Koichi Watashi, Takaji Wakita, Kei Iida, Kei Iida, Hironori Yoshiyama, Hisashi Iizasa

“…Current treatments for hepatitis B virus (HBV), such as interferons and nucleic acid analogs, have limitations due to side effects like depression and the development of drug-resistant mutants, highlighting the need for new therapeutic approaches. …”
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Replication-IDentifier links epigenetic and metabolic pathways to the replication stress response by Sophie C. van der Horst, Leonie Kollenstart, Amandine Batté, Sander Keizer, Kees Vreeken, Praveen Pandey, Andrei Chabes, Haico van Attikum

“…During Repli-ID, the replicative polymerase epsilon (Pol ε) is tracked at a barcoded origin of replication by chromatin immunoprecipitation (ChIP) coupled to next-generation sequencing of the barcode in thousands of hydroxyurea-treated yeast mutants. Using this approach, 423 genes that promote Pol ε binding at replication forks were uncovered, including LGE1 and ROX1. …”
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Development of a Model Mutagenesis System for Snapdragon by Zhaoyuan Lian, Heqiang Huo, Sandra B. Wilson, Jianjun Chen

“…For example, snapdragon pigmentation mutants produced by transposon (a type of mobile DNAs) mutagenesis have provided researchers a good way to study anthocyanin biosynthesis and subsequently aid plant breeders in developing new varieties with novel flower colors (Jackson et al. 1992). …”
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Development of a Model Mutagenesis System for Snapdragon by Zhaoyuan Lian, Heqiang Huo, Sandra B. Wilson, Jianjun Chen

“…For example, snapdragon pigmentation mutants produced by transposon (a type of mobile DNAs) mutagenesis have provided researchers a good way to study anthocyanin biosynthesis and subsequently aid plant breeders in developing new varieties with novel flower colors (Jackson et al. 1992). …”
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ATP-Binding Pocket-Targeted Suppression of Src and Syk by Luteolin Contributes to Its Anti-Inflammatory Action by Jeong-Oog Lee, Deok Jeong, Mi-Yeon Kim, Jae Youl Cho

“…Overexpression of wild type Src and point mutants thereof, and molecular modelling studies, suggest that the ATP-binding pocket may be the luteolin-binding site in Src. …”
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Antibacterial action of penicillin against Mycobacterium avium complex by D. Deshpande, G. Magombedze, S. Srivastava, T. Gumbo

“…Data were analyzed using the inhibitory sigmoid maximal effect (E max) model for each sampling day, while two ordinary differential equations (ODEs) were used for the wild-type and penicillin-resistant mutants. RESULTS: Benzylpenicillin killed >2.1 log 10 colony-forming unit (CFU)/mL below Day 0, better than azithromycin, ethambutol, and rifabutin. …”
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Cereal inflorescence: features of morphology, development and genetic regulation of morphogenesis by O. B. Dobrovolskaya, A. E. Dresvyannikova

“…The genes involved in the control of the cereal inflorescence development have been identified using mutants (mainly maize and rice) with altered inflorescence and floret morphology; most of these genes regulate the initiation and fate of meristems. …”
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Bem-estar subjetivo e comprometimento com a carreira: examinando suas relações entre professores de ensino superior by Rayana Santedicola Andrade, Sônia Regina Pereira Fernandes, Antônio Virgílio Bittencourt Bastos

“…Os estudos sobre comprometimento no trabalho destacam a relação com a carreira como um foco cada vez mais significativo na vida das pessoas, cujos ambientes de trabalho estão cada vez mais incertos e mutantes. Esta pesquisa buscou examinar as associações entre o comprometimento com a carreira e o bem-estar subjetivo, em professores universitários. …”
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Deubiquitinase Ubp5 is essential for pulmonary immune evasion and hematogenous dissemination of Cryptococcus neoformans by Guizhen Wang, Yan Liu, Qianyu Li, Changhui Wang, Wei Fang

“…Under host-associated conditions, ubp5Δ mutants exhibited morphological changes, including distorted shapes and cell wall heterogeneity, alongside defects in key virulence factors such as the polysaccharide capsule and melanin. …”
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Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7- and pH-dependent manner by Qi-Zhang Liang, Chun-Miao Ji, Bin Wang, Wei Chen, Feng Cong, Yu Huang, Yao-Wei Huang

“…Pseudovirus entry was suppressed by means of pharmacological inhibitors, dominant-negative mutants, and siRNAs targeting various cellular proteins and signalling molecules, suggesting that PDCoV and avian DCoV pseudovirus entry into DF-1 cells depends on clathrin, dynamin-2, cathepsins and a low-pH environment but is independent of caveolae and macropinocytosis. …”
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Research Progress on the Structure and Function, Immune Escape Mechanism, Antiviral Drug Development Methods, and Clinical Use of SARS-CoV-2 M^pro by Jiayi Ren, Zhengfu Zhang, Yi Xia, Daqun Zhao, Dingqin Li, Shujun Zhang

“…There is an urgent need to identify a relatively stable target for the development of universal vaccines and drugs that can effectively combat both SARS-CoV-2 strains and their mutants. Currently, the main focus in treating SARS-CoV-2 lies in disrupting the virus’s life cycle. …”
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β-Glucosidase VmGlu1 is required for toxin production and pathogenicity of Valsa mali by Xinyue Cui, Dewan Zhang, Liyong Gao, Na Liu, Sen Lian, Weichao Ren, Baohua Li, Caixia Wang

“…Furthermore, we verified that VmGlu1 and VmGlu2 exhibit functional redundancy in pycnidia production, toxin-related β-glucosidase activity, and pathogenicity by generating double-deletion mutants. The results of this study provide valuable insights into the roles of VmGlus in the interaction of V. mali and apple during the infection process.…”
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“Sichuanvirus”, a novel bacteriophage viral genus, able to lyse carbapenem-resistant Klebsiella pneumoniae by Juan Li, Qingqing Fang, Huan Luo, Yan Feng, Yu Feng, Zhiyong Zong

“…We also obtained three bacterial mutants resistant to the phage and performed genome sequencing for them. …”
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Aportes a la Técnica de Insecto Estéril: Una cepa de Anastrepha fraterculus (Diptera: Tephritidae) con maduración sexual temprana by P.A. Peralta, F.H. Milla, J.L. Cladera

“…Asimismo, se estudió el efecto del tratamiento tópico con hormona juvenil (HJ) sobre la madura- ción sexual en estos machos mutantes. El tiempo que tardan los individuos en alcanzar la madurez sexual fue determinado mediante observaciones del comportamiento sexual, donde machos de distintas edades fueron confrontados con hembras vírgenes sexualmente maduras. …”
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Palmitic Acid Accelerates Endothelial Cell Injury and Cardiovascular Dysfunction via Palmitoylation of PKM2 by Yu He, Senlin Li, Lujing Jiang, Kejue Wu, Shanshan Chen, Linjie Su, Cui Liu, Peiqing Liu, Wenwei Luo, Shilong Zhong, Zhuoming Li

“…Palmitoyl‐proteomics analysis identifies pyruvate kinase isozyme type M2 (PKM2) as the palmitoylated protein responsible for PA‐induced endothelial injury, and Cys31 as the predominant palmitoylated site. PKM2‐C31S mutants (cysteine replaced by serine) prevents PA‐induced endothelial injury. …”
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